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Citations for BioLegend :
1101 - 1150 of 6900 citations for Nuclear pore complex protein Nup93 dye Antibody Biotin since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... The pelleted cells were resuspended in FACS buffer (PBS + 1% bovine serum albumin + 2 mM EDTA + 0.1% sodium azide) containing 1:100 dilution of Fc Block and a 1:250 dilution of Zombie Violet dye (423113; Biolegend). After incubation at 4°C for 15 minutes ...
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bioRxiv - Microbiology 2024Quote: ... vaginal cells were first stained for cell viability using Fixable Viability Dye eFluor506 (Thermo-Fisher) and then subsequently stained with anti-CD45-BV421 (clone 30-F11, BioLegend), anti-CD4-PE-Cy5 (clone RM4-5 ...
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bioRxiv - Immunology 2024Quote: Single cell suspensions were washed with PBS and resuspended in 100 μL Zombie aqua cell viability dye solution (1:400 prepared in PBS, Cat. No. 423101, BioLegend) and incubated at room temperature for 15-30 minutes ...
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bioRxiv - Immunology 2024Quote: Aliquots of 5-10 x 106 cells were first assessed for viability staining using eFlour780 (eBioscience) fixable viability dye (1:1000) or 7AAD (Biolegend) viability dye (1:100) ...
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bioRxiv - Immunology 2024Quote: ... cells were stained with the Fixable Viability Dye eFluor 506 (65-0866-14; eBioscience) followed by a staining with FoxP3 AF647 (320113; Biolegend) and IL-34 PE (IC5265P ...
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bioRxiv - Bioengineering 2024Quote: ... The cells were then washed twice with PBS before being incubated with Zombie NIR Fixable Viability dye (1:1000, BioLegend) for 15 min at RT ...
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bioRxiv - Bioengineering 2024Quote: Lung cell suspensions prepared above were resuspended in 1 mL of PBS containing Zombie Green Viability Dye (0.1% Biolegend, 423112) and incubated at 4°C for 15 min ...
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bioRxiv - Cancer Biology 2024Quote: ... We stained cells for 15 minutes on ice with Fixable Viability Dye eFluor 780 (eBioscience) or Fixable Viability Dye eFluor 506 (eBioscience) to differentiate between live and dead populations and ɑCD16/CD32 (BioLegend) to prevent non-specific antibody binding ...
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bioRxiv - Neuroscience 2022Quote: ... Antibodies (BioLegend) used were as follows:
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bioRxiv - Cancer Biology 2021Quote: ... The samples were blocked with 0.5 % BSA for 10 min on ice and incubated with the primary mAbs biotin-anti-human CD274 (B7-H1, PD-L1) (clone 29E.2A3, BioLegend, San Diego, CA, USA) or biotin anti-mouse CD274 (clone 10F.9G2 ...
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bioRxiv - Cell Biology 2022Quote: ... Antibodies used were pericentrin (PCNT) antibody (PRB-432C-Biolegend, Covance Cat# PRB-432C ...
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bioRxiv - Genomics 2020Quote: ... oligonucleotide-conjugated antibodies and fluorescently labeled antibodies (Biolegend, USA) were both added at a 1:100 dilution in the same buffer as in the initial permeabilization step and incubated for 1h ...
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bioRxiv - Plant Biology 2023Quote: ... Then 4 µg of primary antibodies (αHA antibody: Biolegend, #901502 ...
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bioRxiv - Molecular Biology 2020Quote: ... and LCL A382T TDP-43 mutated patients (LCL-TDP382) were incubated with a vitality dye for 15 minutes (Zombie Violet™ Fixable Viability Kit, BioLegend). Then ...
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bioRxiv - Cell Biology 2021Quote: Live single-cell suspensions were labelled with Zombie NIR™ fixable viability dye (Cat# 423105, BioLegend®, San Diego, CA, USA) to eliminate dead cells during the analysis stage ...
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bioRxiv - Cancer Biology 2022Quote: ... and Zombie Dye Violet Blue (Biolgend; 423114) for 15 minutes at 4degC followed by surface labeling with PE anti-human CD45 (Biolegend; 304008) for 30 minutes at 4degC ...
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bioRxiv - Molecular Biology 2022Quote: ... an equal number of cells from each group were incubated at room temperature for 20 minutes with Zombie Aqua™ Fixable Viability dye (BioLegend) and blocked on ice for 20 minutes with FcBlock anti-CD16/CD32 (Thermo Fisher) ...
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bioRxiv - Immunology 2020Quote: ... Cells were subject to FSc and SSc doublet discrimination and dead cells were excluded from analysis using Zombie NIR viability dye (Biolegend #423106).
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bioRxiv - Microbiology 2021Quote: The lung cells were washed and resuspended in 100 μL PBS and stained with a fixable viability dye (Zombie Aqua™, 423101; BioLegend) for 10 min at room temperature protected from light ...
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bioRxiv - Immunology 2022Quote: Cryopreserved PBMCs were thawed and washed twice with 10 ml of FACS buffer (1 x PBS containing 2% FBS and 1 mM EDTA) and resuspended in 100 μl of 1x PBS containing Zombie UV live/dead dye at 1:200 dilution (BioLegend, 423108) and incubate at room temperature for 15 minutes ...
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bioRxiv - Immunology 2021Quote: ... Cells were stained for 10 minutes at room temperature with Ghost Dye Violet 510 and Fc receptor blocking solution (Human TruStain FcX™, BioLegend) and washed once in FACS buffer ...
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bioRxiv - Genetics 2021Quote: ... Cells were then washed twice with cold PBSx1 and the cells were incubated with live/dead Zombie Violet Dye (Biolegend, 423114) for 15 min at RT as suggested by the manufacturer ...
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bioRxiv - Microbiology 2022Quote: ... bacteria were labeled with 5 µg ml−1 carboxylfluorescein diacetate succinimidyl ester (CFSE) or Tag-IT Violet™ proliferation and cell tracking dye (Biolegend) in PBS containing 5 mM MgSO4 ...
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bioRxiv - Cancer Biology 2022Quote: Primary mouse and human cells were isolated as described above and stained with Zombie fixable viability dye for 20 min at room temperature (#423102, BioLegend, USA) and pre-incubated with anti-mouse Fc block at 10 μg mL-1 (#101320 ...
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bioRxiv - Developmental Biology 2024Quote: Single-cell suspensions were obtained at day 2.5 of differentiation Cells were first labeled with Zombie NIR fixable viability dye (BioLegend, 1:500) for the exclusion of dead cells ...
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bioRxiv - Immunology 2023Quote: ... Cells were incubated in 200 μl FACS buffer containing 1 μl Zombie NIR fixable dye for 30 min to determine cell viability (Zombie Green™ Fixable Viability Kit, BioLegend). Cells were fixed for 15 min in 200 μl PFA 2 % ...
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bioRxiv - Immunology 2023Quote: Lung cells prepared as described above were counted and washed with cold PBS twice before staining with 1:200 Zombie Aqua Fixable Viability Dye (BioLegend, 423101) for 15 min at 4 °C ...
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bioRxiv - Immunology 2023Quote: ... The sample pool was washed once with 10 mL of PBS-BSA and resuspended in 150 µL of Zombie NIR viability dye (1:500 in PBS; BioLegend 423105). After 15 minutes on ice ...
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bioRxiv - Bioengineering 2023Quote: ... Samples were subsequently stained with Zombie Violet (1:400) viability dye and APC-Cy7 conjugated human anti-CD45 (BioLegend, clone: 2D1) for 45 minutes at 4°C protected from light ...
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bioRxiv - Immunology 2023Quote: ... stained for 10 minutes at room temperature with Ghost Dye Violet 510 and Fc receptor blocking solution (Human TruStain FcX™, BioLegend), and washed once in FACS buffer ...
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Fine tuning of CpG spatial distribution with DNA origami for improved therapeutic cancer vaccinationbioRxiv - Synthetic Biology 2023Quote: BMDC or T cell suspensions collected as described above were washed with PBS and stained with Zombie UV viability dye (BioLegend, #423108) and then washed with cell staining buffer (BioLegend ...
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bioRxiv - Microbiology 2023Quote: ... 1.5 million cells were washed twice with PBS prior to 15 min of staining with the Zombie NIRTM Fixable Viability dye (BioLegend – 423105), followed by 2 washes with FACS Buffer (PBS ...
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bioRxiv - Neuroscience 2023Quote: Endothelial cells isolated from brain from SARS-CoV-2 infected mice were stained with fixable viability dye (Zombie Green, Biolegend 423111) and blocked with Fc receptor blockade (anti-mouse CD16/32 ...
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bioRxiv - Synthetic Biology 2024Quote: ... washed once with 1X PBS and incubated with Zombie NIR viability dye (1:750, Biolegend, San Diego, CA, USA; cat#423105) in PBS for 20 min ...
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bioRxiv - Immunology 2024Quote: ... Cells were stained with Zombie NIR viability dye for 10-15 minutes at room temperature (Biolegend, Cat. 423106, 1:1000 dilution), followed by quenching and washing with FACS buffer ...
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bioRxiv - Immunology 2024Quote: Intratumoral CD8+ T cell sample isolated from tumor from each mouse was stained with Zombie Aqua Fixable Viability Dye (Biolegend, #423101) in 1x PBS on ice for 15 min ...
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bioRxiv - Immunology 2023Quote: Biotinylated S1 protein was obtained commercially (BioLegend). A 4X molar excess was incubated with streptavidin-Alexa Fluor 647 (0.5 mg/mL ...
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bioRxiv - Neuroscience 2023Quote: ... the following primary antibodies and secondary antibodies were used: mouse anti-HA antibody (1:100) (16B12, BioLegend), rabbit anti-HA antibody (1:200 ...
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bioRxiv - Cell Biology 2024Quote: ... the following antibodies were used (all antibodies were from BioLegend): 1:200 FITC-conjugated anti-CD45 ...
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bioRxiv - Cell Biology 2024Quote: ... the following antibodies were used (all antibodies were from BioLegend): 1:100 AF488-conjugated anti-F4/80 ...
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bioRxiv - Neuroscience 2023Quote: ... At the antibody labelling stage oligonucleotide tagged antibodies (Hashtags, Biolegend) were added with the other cell type specific antibodies ...
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bioRxiv - Immunology 2024Quote: Antibody staining mix contained antibodies against CD38-AF700 (Biolegend, 102742), CD138-BB515 (BD ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... antibodies after blocking by anti-CD16/32 antibodies (BioLegend 101302). Dead cells were excluded via 7-aminoactinomycin D (Wako ...
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bioRxiv - Immunology 2021Quote: Single-cell suspensions prepared from lungs were washed twice with PBS prior to incubating with Zombie UV™ Fixable Viability Dye and TruStain FcX™ (clone 93; both from BioLegend) for 15 minutes at room temperature ...
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bioRxiv - Cancer Biology 2022Quote: ... Approximately 5×105 cells were stained for 30 min using the Zombie Aqua fixable viability dye (BioLegend Cat# 423101, 1:500 dilution), then fixed for 1 h in Foxp3/Transcription Factor Fixation/Permeabilisation Solution (ThermoFisher Cat# 00552300) ...
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bioRxiv - Immunology 2023Quote: Single-cell suspensions prepared from lungs were washed twice with PBS prior to incubating with Zombie UV™ Fixable Viability Dye and TruStain FcX™ (clone 93; both from BioLegend) for 15 minutes at room temperature ...
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bioRxiv - Immunology 2023Quote: ... Cells were resuspended in PBS and stained at 4°C with 100 µL 1:500 Zombie Aqua viability dye (BioLegend, Cat# 423102) and 1:200 mouse FC block (TruStain FcX ...
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bioRxiv - Cancer Biology 2024Quote: ... for 2 minutes at room temperature and then cells were washed in PBS and incubated for 15 minutes at room temperature with a PBS solution containing the fixable viability dye Zombie VioletTM (Biolegend, 1:100) and CD16/CD32 Fc Block (BD Biosciences ...
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bioRxiv - Immunology 2024Quote: ... Cryopreserved PBMC were thawed on ice and incubated with 100 μL of Zombie Live/Dead dye (diluted 1:400 in PBS) (Biolegend, London, UK) for every 5×106 cells at room temperature for 20 minutes ...
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bioRxiv - Microbiology 2020Quote: ... coli antibody (BioLegend). Densitometry analysis was performed on an AlphaImager HP system using the manufacturer’s supplied AlphaView software ...