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Citations for BioLegend :
1101 - 1150 of 8306 citations for HLA A*0201 NY ESO 1 complex Protein Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: Supernatants from stimulated T cells were harvested on day 2 and day 6 depending on peak of cytokine production and analyzed using the LEGENDplexTM human Thelper 1/2/9/17 multiplex kit (BioLegend). Beads were acquired on a BD Canto II (BD Bioscience ...
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bioRxiv - Immunology 2023Quote: ... stained with a mixture of fluorochrome-conjugated antibodies (1:200 FITC anti-mouse/human CD11b M1/70, BioLegend, California, USA), (1:200 APC/Cyanine7 anti-mouse F4/80 ...
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bioRxiv - Cancer Biology 2023Quote: ... and then incubated for one hour in PE anti-human CD71 Antibody in the dark at 4°C (1:100; Biolegend). Cells were washed twice with PBS and then and then analysed using a flow cytometer (FACsFortessa ...
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bioRxiv - Bioengineering 2023Quote: ... followed by staining with Brilliant Violet (BV) 785 conjugated anti-human CD69 (10 μgꞏml−1) (Biolegend, Cat # 310932, clone FN50) for 30 min on ice ...
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bioRxiv - Genomics 2023Quote: ... and all the samples were treated with sybr green (1x) and mouse anti-human IgG conjugated with Alexflour 647 (1:100 dilution, from BioLegend) for 30 mins at room temperature ...
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bioRxiv - Immunology 2023Quote: ... were resuspended in labeling buffer (1% BSA in PBS) and underwent Fc blocking for 10 minutes on ice using Human TruStain FcX (BioLegend). Next ...
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bioRxiv - Microbiology 2023Quote: ... 100,000 cells were plated in 96-well round bottom plate and blocked with 1:1000 Human TruStain FcX™ (Biolegend) diluted in washing buffer for 10 minutes ...
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bioRxiv - Immunology 2023Quote: ... the following antibodies were used at a 1:200 ratio: epithelial cells (324216, Biotin anti-human Epcam, clone:9C4, BioLegend), endothelial cells (13-0319-82 ...
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bioRxiv - Immunology 2023Quote: ... B cells were gated for using 1:20 dilution commercial mouse anti-human CD19-Alexa488 (BioLegend, San Diego, CA, USA) and 1:20 dilution mouse anti-human CD27-PE (stock 50 ug/mL ...
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bioRxiv - Immunology 2023Quote: ... were derived from a cell sort that included an additional cell hashing step (TotalSeq-C0251/C0252/C0253/C0254/C0255 anti-human Hashtag 1/2/3/4/5 antibodies, cat # 394661/394663/394665/394667/394669, Biolegend) prior to B cell enrichment and staining ...
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bioRxiv - Genomics 2023Quote: ... A subset of the hiPSC lines were evaluated by flow cytometry for expression of two pluripotent markers: Tra-1-81 (Alexa Fluor 488 anti-human, Biolegend) and SSEA-4 (PE anti-human ...
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bioRxiv - Genomics 2023Quote: ... Many of the iPSC lines were evaluated by flow cytometry for expression of two pluripotent markers: Tra-1-81 (Alexa Fluor 488 anti-human, Biolegend) and SSEA-4 (PE anti-human ...
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bioRxiv - Genomics 2024Quote: ... A subset of the iPSC lines was evaluated by flow cytometry for expression of two pluripotent markers: Tra-1-81 (Alexa Fluor 488 anti-human, Biolegend) and SSEA-4 (PE anti-human ...
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bioRxiv - Immunology 2023Quote: ... 200 μl for 96-well plates) supplemented with 1 μg/ml of recombinant human B cell-activating factor (BAFF, BioLegend). 1 µg/ml rHA from H1N1 (PR8 ...
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bioRxiv - Cell Biology 2024Quote: ... collected in PBS + 1% FBS and stained for ICAM-1 expression on the endothelial cell surface using APC anti-human CD54 antibody (353111, Biolegend). Flow cytometry analysis was performed and confirmed that ICAM-1 expression was upregulated following overnight stimulation with TNF in each of the co-culture experiments.
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bioRxiv - Immunology 2019Quote: ... 0.2% BSA in PBS with 0.02% sodium azide), blocked with human (humans, NHPs) or mouse (mice) TruStain FcX block (Biolegend) for 10 minutes at room temperature with shaking ...
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bioRxiv - Immunology 2021Quote: ... IL-10 in human cell culture supernatants were measured using the LEGEND Max Human IL-10 ELISA kit (BioLegend). Technical duplicates were averaged and then normalized to the total number of live cells in each sample at the time of collection.
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bioRxiv - Cancer Biology 2022Quote: ... or TFH mimicking signals (including 0.02 μg/mL recombinant human IL-4, Peprotech; 0.05 μg/ml recombinant human IL-21, Peprotech, and 5 μg/mL anti-human CD40, Biolegend). Complete RPMI medium contained RPMI 1640 (Gibco ...
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bioRxiv - Immunology 2020Quote: ... Monoclonal mouse IgG1 anti-human CD64 FITC-conjugated (clone 10.1) and monoclonal mouse IgG2a anti-human CD59 PE-conjugated were from BioLegend, San Diego ...
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bioRxiv - Bioengineering 2022Quote: ... 100 IU/mL recombinant human IL-2 (Proleukin, Prometheus Laboratories) and 5 ng/mL recombinant human IL-7 (BioLegend) at 37C ...
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bioRxiv - Immunology 2023Quote: ... Human Th17 differentiation assays were performed similarly except the concentrations of recombinant human IL-6 (30 ng/ml, BioLegend), TGF- β1 (2.25ng/mL) ...
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bioRxiv - Immunology 2023Quote: Activation consisted of 10μg/mL plate-bound anti-human CD3 antibody (300437) and 1μg/mL soluble anti-human CD28 antibody (302902) purchased from BioLegend, USA ...
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bioRxiv - Neuroscience 2022Quote: ... mouse anti-myelin-associated glycoprotein (MAG, 1:100, SigmaAldrich MAB1567),chicken anti-glial fibrillary acidic protein (GFAP, 1:500, BioLegend PCK-591P), chicken anti-GFAP (1:500 ...
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bioRxiv - Immunology 2021Quote: ... Bound ligand was detected with a horseradish peroxidase (HRP)-conjugated anti-His antibody (652504, BioLegend, San Diego, CA, USA). After incubation for 1 hour at room temperature and washing ...
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bioRxiv - Bioengineering 2022Quote: ... were coated with 100ng per well of recombinant Spike RBD protein in 1×coating buffer (Biolegend) overnight at 4C° ...
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bioRxiv - Cell Biology 2023Quote: ... proteins were labeled with primary antibodies against CD9 (1:1,000; clone HI9, Biolegend, San Diego, USA), CD44 (1:400 ...
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bioRxiv - Immunology 2023Quote: ... BA.1 or BA.2 biotinylated RBD protein conjugated with TotalSeq™-C0971 Streptavidin (BioLegend, 405271) and TotalSeq™-C0972 Streptavidin (BioLegend ...
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bioRxiv - Neuroscience 2020Quote: For analysis of LILRB2 expression on marmoset and human monocyte derived macrophages were first blocked with human TruStain FcX Fc blocking solution (Biolegend), then labelled with and without anti-human LILRB2 (5 µg/mL ...
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bioRxiv - Bioengineering 2021Quote: ... Naïve T cell marker staining was performed with APC/Cy7 mouse anti-human CD45RA (#304128) and BV510 mouse anti-human CD45RO (#304232) antibodies from Biolegend.
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bioRxiv - Immunology 2021Quote: ... Tonsillar IgD+ B cells were isolated from human tonsil cells by positive selection using biotin-anti-human IgD mAb (clone IA6-2; 348212, Biolegend) and MagniSort™ Streptavidin Positive Selection Beads (MSPB-6003-74 ...
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bioRxiv - Immunology 2022Quote: ... PBMCs were surface stained with series of antibodies (anti-human CD158 [KIR2DL1/S1/S3/S5]-FITC [BioLegend, Cat#339504], anti-human CD158b [KIR2DL2/L3, NKAT2]-FITC [BioLegend, Cat#312604] ...
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bioRxiv - Immunology 2022Quote: ... PBMC derived T cells were stimulated for five days at 37°C with 1μg/mL anti-CD3 and anti-CD28 and 10ng/mL of recombinant human IL-2 and recombinant human IL-12p70 (BioLegend). On day four of stimulation ...
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bioRxiv - Biophysics 2022Quote: ... Monoclonal antibodies against human Glycophorin AB (CD235ab) (HIR2; catalog number: 306602) and human CD47 (CC2C6; catalog number: 323102) were purchased from BioLegend.
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bioRxiv - Immunology 2019Quote: ... Supernatants were analyzed by using either the Legendplex Multi-Analyte Flow Assay Kit for human CD8/NK cells or the human Th cytokine panel (Biolegend).
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bioRxiv - Immunology 2021Quote: ... containing anti-CD9 (human HI9a, mouse MZ3), anti-CD63 (human H5C6, mouse NVG-2), anti-CD81 antibodies (human 5a6, mouse Eat-2) from Biolegend, and anti-human ACE2 (R&D Biosystems cat no ...
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bioRxiv - Microbiology 2021Quote: ... Mouse IgG and human IgG were detected with separate secondary antibodies (goat anti-human IgG Fc, goat anti-mouse IgG Fc, BioLegend) at a dilution of 1:4,000 in blocking buffer and PNPP as described above.
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bioRxiv - Immunology 2020Quote: ... For human cytokine IL-4 in the supernatant of human ILC2 cell cultures were measured by ELISA kit from BioLegend. All final reactions were developed with TMB substrate (Thermo scientific ...
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bioRxiv - Immunology 2021Quote: ... fluorochrome-conjugated antibodies (anti-mouse CD45-BioLegend Cat. No. 103126, anti-human CD45-BioLegend Cat. No. 304014, anti-human CD3-BioLegend Cat ...
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bioRxiv - Immunology 2020Quote: ... human Interlukin-6 (IL-6) (100 ng/ml) and human IL-6Rα (100 ng/ml) (BioLegend, San Diego, CA, USA).
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bioRxiv - Cell Biology 2022Quote: ... Cells were incubated with antibodies directly conjugated to fluorescent dyes (anti-human BAFF-R conjugated to FITC, BioLegend, Cat. #: 316904; anti-human BCMA conjugated to APC, BioLegend, Cat ...
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bioRxiv - Immunology 2022Quote: The untouched resting B cells were isolated from the human tonsils by depletion of other cells using MojoSort Human B Cell (CD43-) Isolation Kit (BioLegend), whereas the naïve B cells were isolated using the EasySep Human Naïve B cell isolation kit (Stem Cell Technologies) ...
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bioRxiv - Immunology 2023Quote: ... FITC anti-human CD18 antibody and FITC anti-human CD11c antibody (clone Bul15) were purchased from BioLegend (San Diego, USA). Rabbit anti-human MERTK antibody (D21F11 ...
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bioRxiv - Immunology 2023Quote: ... THP-1 and SKOV-3 cell lines and primary human T cells were labeled with fluorescent antibody against human CD70 or isotype control (Biolegend) to verify antigen expression as described before35.
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bioRxiv - Immunology 2023Quote: ... Cells were analyzed and sorted for transfection efficiency via flow cytometry analysis using anti-human CD3 APC/Cy7-conjugated antibody and anti-human CD34 PE-conjugated antibody for TCR-expressing cells (BioLegend, 300426 at 10 μg/mL and BioLegend ...
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bioRxiv - Microbiology 2024Quote: CD14+ monocytes were isolated from PMBCs of healthy human blood using the MojosortTM Human CD14 Selection Kit (BioLegend, Cat# 480026) To generate monocyte derived dendritic cells (moDCs) ...
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bioRxiv - Microbiology 2020Quote: ... stained with anti-human CD14 (Clone 63D3; Biolegend) and quantified using Attune NxT (Life Technologies) ...
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bioRxiv - Cell Biology 2020Quote: ... BV421 anti-human CD14 (325627, clone HCD14, BioLegend), PE-Cy7 anti-human CD123 (560826 ...
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bioRxiv - Cell Biology 2020Quote: ... FITC anti-human Fas/CD95 (Clone DX2, Biolegend), APC anti-human Nectin-2/CD112 (Clone TX31 ...
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bioRxiv - Immunology 2022Quote: ... and the MojoSort Human CD8 T cell (BioLegend) isolation kit ...
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bioRxiv - Cancer Biology 2019Quote: ... anti-human CD133 and anti-ICAM1 antibodies (BioLegend). A549 and H460 cells pretreated with DMSO ...