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Citations for BioLegend :
1101 - 1150 of 1164 citations for Caspase 3 p12 Rabbit Recombinant mAb since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2019Quote: ... clones that survived after 3-4 months of culture were washed with PBS and stained with FITC anti-HLA-DQ (Biolegend, Clone: HLADQ1) for 30 minutes on ice and cell surface expression assessed by flow cytometry ...
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bioRxiv - Immunology 2021Quote: ... plates were coated at least 5 hours prior to use with 3 ug/ml plate-bound anti-CD3 antibody (clone OKT3; BioLegend, LEAF grade), 1 ug/ml soluble anti-CD28 antibody (BioLegend ...
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bioRxiv - Immunology 2022Quote: ... Cytokine staining of splenocytes after attLm-OVA infection was carried out after re-stimulation of CD8 T cell enriched splenocytes with 50 nM SIINFEKL peptide for 3 hours with Brefeldin A (Biolegend, cat#:420601).
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bioRxiv - Neuroscience 2022Quote: ... and 0.3% PBST for 3 days at 4°C on rotating incubator (NFH, 1:500, Abcam, ab4680; Tubulin β-III, 1:200, Biolegend, 801201). Whole muscles were washed in 0.3% PBST for 5 hours then incubated in secondary antibodies for 48h at 4°C on rotating incubator (AlexaFluor 568 ...
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bioRxiv - Immunology 2023Quote: ... Supernatants were collected 3 days after coculture initiation and used to quantify T cell activation levels with ELISA (BioLegend, Cat no. 430801) following the manufacturer’s protocols.
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bioRxiv - Immunology 2023Quote: ... then were resuspended in FACS (PBS + 3% FBS + 1mM EDTA + 10mM HEPES) supplemented with TruStain FcX (1:50; BioLegend; San Diego, CA) and TruStain Monocyte Blocker (1:20 ...
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bioRxiv - Immunology 2024Quote: ... Primary lymphocytes isolated from skin draining lymph nodes from adult C57BL/6 mice were cultured on plates precoated with anti-mouse α-CD3 (3 μg/ml, Biolegend, #100340) and anti-mouse α-CD28 (3 μg/ml ...
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bioRxiv - Cell Biology 2020Quote: ... Rabbit polyclonal antibody directed against Myosin-IIA (#909801, IF dilution 1:100) was from Biolegend (Princeton, NJ, USA). Mouse monoclonal antibody directed against α1-actinin (#TA500072S ...
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bioRxiv - Immunology 2020Quote: ... Cells were then incubated with an anti-rabbit DyLight™649-conjugated donkey secondary Ab [clone Poly4064 (BioLegend)] for 30 minutes at room temperature (1:500 dilution) ...
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bioRxiv - Biophysics 2022Quote: ... the NMMIIA rods were labeled using 909801 anti-non-muscle-myosin IIA heavy chain primary antibody (Biolegend, rabbit) and Alexa 561 secondary (goat anti-rabbit).
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bioRxiv - Developmental Biology 2021Quote: The following antibodies and dilutions were used: rabbit anti-transformation related protein 63 (P63, 1:250, 619002, Biolegend), rabbit anti-red fluorescent protein (tdT ...
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bioRxiv - Cancer Biology 2023Quote: ... The primary antibodies that were used in this study included rabbit anti- human CD271 (1:100, HPA004765, Biolegend), mice anti-human CXCL12 (1:50 ...
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bioRxiv - Bioengineering 2021Quote: ... T cells were similarly isolated and stained with Ghost Dye and lineage/differentiation markers (without CD19) and with TIM-3 BV711 (Biolegend clone F38-2E2), LAG-3 BV421 (Biolegend clone 11C3C65) ...
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bioRxiv - Cancer Biology 2024Quote: ... Spleenocytes were resuspended in 3 ml MojoSort™ buffer and enriched for CD8+ T cells using the MojoSort™ Mouse CD8 T Cell Isolation Kit (BioLegend, 480035), following manufactureŕs instructions.
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bioRxiv - Cell Biology 2020Quote: ... containing 1% BSA and incubated with rabbit monoclonal CTR1 antibody (ab129067) or an isotype control antibody (Biolegend, clone Poly29108) for 1 hour on ice ...
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bioRxiv - Neuroscience 2021Quote: ... Cells overexpressing Rab35 were detected using an anti-HA primary antibody (data not shown; Rabbit, Cell Signaling; Mouse, Biolegend) + Alexa Fluor-405 secondary antibody ...
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bioRxiv - Neuroscience 2022Quote: ... Cochlea sections were also immunolabeled with rabbit anti-β3 tubulin (1:1000; clone TUJ1, Biolegend, San Diego, CA, USA) overnight at 4 °C followed by goat anti-rabbit Alexa-488 (1:200 ...
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bioRxiv - Immunology 2023Quote: ... Cells were incubated with phospho-EZH2 (S21) (Affinity Biosciences, Cat# AF3822) or rabbit IgG isotype control (BioLegend, Cat# 910801) followed by PE-conjugated anti-rabbit antibody (BioLegend ...
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bioRxiv - Cell Biology 2023Quote: ... The slides were incubated with a primary rabbit anti-MMP2 antibody (cat# 87809, BioLegend, San Diego, CA; 1:100) overnight ...
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bioRxiv - Cell Biology 2023Quote: ... an RBD - HC2.RBD.rFC (Metafora – 1:5) was used in conjunction with Alexa 647 donkey anti rabbit Poly4064 (1:50 Biolegend).
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The spindle protein CKAP2 regulates microtubule dynamics and ensures faithful chromosome segregationbioRxiv - Cell Biology 2023Quote: ... Primary incubation was done overnight at 4 °C with rabbit anti-CKAP2 1:2000 or mouse anti-GAPDH (BioLegend, 649201 ...
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bioRxiv - Biophysics 2021Quote: Flow channels were constructed as previously described but anti-TAMRA antibody was replaced with Anti-ß3 tubulin antibody (BioLegend Poly18020 anti-Tubulin Beta 3). Microtubule nucleation was achieved by introducing 5-50 μM tubulin (1:17 molar ratio of Atto-633 labelled:unlabelled ...
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bioRxiv - Genomics 2019Quote: ... The following antibodies were used for these studies: anti-human CD47-APC (1µl/106 cells in 100µl; clone CC2C6, BioLegend, Cat. #323123/4, RRID: AB_2716202/3), anti-human CD47-FITC (5µl/106 cells in 100µl ...
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bioRxiv - Cell Biology 2022Quote: ... half a million cells per sample were incubated for 10 min in 50 μl of PBS with 3% FSB and 5 μl of blocking buffer (BioLegend, San Diego, CA, USA) and then stained with 50x diluted TotalSeq-B cell feature barcode antibody (BioLegend ...
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bioRxiv - Cell Biology 2024Quote: ... The following primary antibodies were used in this study: anti-tubulin beta-3 conjugated to Alexa Fluor 488 (BioLegend; AB_2562669; mouse IgG2a, 1:200), Alexa Fluor 555 (BD PharMingen ...
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bioRxiv - Cancer Biology 2023Quote: Other conjugated antibodies used in this study include the following: Alexa Fluor® 647 anti-Tubulin Beta 3 (1:1000, Biolegend, #657406, clone AA10), Alexa Fluor® 488 anti-Vimentin (1:500 ...
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bioRxiv - Neuroscience 2020Quote: ... the neurons were fixed and stained as previously described38 using primary antibodies for β-3-Tubulin (TUJ1; 1:200; BioLegend, San Diego, CA, USA; 801201) and TH (1:300 ...
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bioRxiv - Microbiology 2021Quote: ... the cells were incubated in PBS-5% FCS on ice for 30 min with rabbit anti-K10 (1:1000) (Biolegend), and K10 was visualized with anti-rabbit AF488 (Life Technologies ...
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bioRxiv - Neuroscience 2020Quote: Free floating 30 μm sagittal brain sections were treated for antigen retrieval using sodium citrate buffer and subsequently stained using primary antibodies for Iba1 (1:250, rabbit, Wako) and anti-6E10 (1:1000, Biolegend), and the corresponding secondary antibodies and nuclear counterstain using DAPI ...
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Safety and efficacy of C9ORF72-repeat RNA nuclear export inhibition in amyotrophic lateral sclerosisbioRxiv - Systems Biology 2021Quote: ... cell cultures were incubated with the appropriate primary antibodies (rabbit anti-Nestin, 1:500 [Biolegend #841901]; mouse anti-Pax6, 1:200 [Millipore #MAB5552]; mouse anti-Tuj1, 1:1000 [Biolegend #801201] ...
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bioRxiv - Developmental Biology 2022Quote: ... MGs were localized using the mT/mG reporter or stained using a rabbit anti-K14 polyclonal antibody (1:500, 905301, BioLegend). Nuclei were counterstained with DAPI ...
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bioRxiv - Immunology 2022Quote: ... at a 1:200 dilution for 30 mins at room temperature and was detected with anti-rabbit BV421 (Dnk, BioLegend). Flow was performed on Cytek Aurora (Cytek ...
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bioRxiv - Microbiology 2019Quote: ... The membrane was incubated with anti-ShyA polyclonal antibody (1:5,000, produced by Pocono Rabbit Farm & Laboratory, PA) or monoclonal anti-αRpol antibody (1:15,000, BioLegend cat# 663104) for two hours and then washed with TBST (20 mM Tris-HCl (pH7.8) ...
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bioRxiv - Microbiology 2022Quote: ... using 1:1000 of polyclonal rabbit anti-human MCT1 IgG (Cell Signalling) and 1:1000 mouse anti-human CD147 IgG (Biolegend) followed by HRP-conjugated secondary IgG antibody of specific host type ...
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bioRxiv - Immunology 2023Quote: ... and goat anti-rabbit Alexa Fluor Plus 647-coupled secondary antibody (1:500) in Intracellular Staining Permeabilization Wash Buffer (Biolegend). The fraction of cells positive for cleaved caspase-3 was measured with a BD LSRFortessa SORP flow cytometer ...
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bioRxiv - Immunology 2023Quote: ... Mouse monoclonal anti-V5 (BLD-680605, clone 7/4) and rabbit polyclonal anti-V5 (BLD-903801) were obtained from BioLegend. Mouse monoclonal anti-ICP36 (anti-UL44 ...
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bioRxiv - Cancer Biology 2023Quote: ... mice were injected intraperitoneally with three hundred micrograms of Ultra-LEAF purified anti-Asialo GM1 rabbit polyclonal antibody (clone Poly21460, BioLegend) every four days for the duration of the experiment ...
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bioRxiv - Neuroscience 2023Quote: ... Primary antibodies, rabbit anti-phospho-cofilin (CST, #3311, 1:100) and mouse anti-SMI-32 (BioLegend, # SMI-32R, 1:250), were applied in blocking buffer at 4°C overnight ...
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bioRxiv - Genomics 2019Quote: ... cells were washed once in PBS and once in Flow buffer (PBS with 2% BSA) and stained with anti-human CD47-APC antibody (clone CC2C6, BioLegend, Cat. #323123/4, RRID: AB_2716202/3) in Flow buffer (20µl antibody/40×106 cells/ml ...
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bioRxiv - Bioengineering 2020Quote: ... anti-Pax7 (mouse monoclonal, DHSB, diluted 1:4) and anti-Neurofilament H phospho (rabbit polyclonal, BioLegend # PRB-573C, diluted 1:400). As secondary antibodies ...
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bioRxiv - Neuroscience 2022Quote: ... Primary immunoreaction solution consisted of blocking buffer that contained 1:1000 rabbit anti-melanopsin (ATS Bio AB N38) and 1:250 mouse anti-SMI32 (Biolegend 801701). After incubation in the primary immunoreaction solution ...
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bioRxiv - Cancer Biology 2022Quote: The following primary antibodies with indicated dilution in blocking buffer were used: Rabbit anti-Tuj1 (Covance, BioLegend, MRB-435P, 1:1,000), Mouse anti-Tuj1 (Covance ...
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bioRxiv - Neuroscience 2023Quote: ... Primary antibodies were using 1:500 rabbit anti Abeta 1:500 (IBL, CAT # 18584) and 1:500 mouse anti SMI312 (Biolegend 837904).
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bioRxiv - Developmental Biology 2023Quote: ... anti-Neurod1 (1/500, mouse monoclonal, hybridoma) (Kani et al., 2010), anti-paired box 2 [Pax2] (1/700, rabbit polyclonal) (BioLegend, 901001), anti-Foxp1b ...
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bioRxiv - Microbiology 2023Quote: ... The membranes were then incubated in 1×PBST with either rabbit anti-flagellin (kindly given by Dr Monica Rolando) or mouse anti-RNAPα (BioLegend #663104) diluted 1/5,000 for 1h at room temperature with agitation ...
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bioRxiv - Bioengineering 2024Quote: ... cells were washed and stained with an Allophycocyanin (APC) labeled secondary antibody (Donkey anti-rabbit IgG DyLight™ 649, Biolegend 406406) for 30-45 minutes at room temperature in 2% normal donkey serum ...
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bioRxiv - Immunology 2024Quote: ... then treated with fluorophore-conjugated secondary antibody (donkey anti-rabbit IgG Alexa Fluor 488, Invitrogen A32790TR; goat anti-hamster IgG Alexa Fluor 594, BioLegend B388503) diluted 1:1000 in blocking buffer for 1hr at RT ...
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bioRxiv - Microbiology 2020Quote: ... Cells were immunostained with the following antibodies: rabbit anti-IFITM3 (Cat. nr. AP1153a, Abcepta, 1:500) and mouse anti-HA (clone 16B12, BioLegend, 1:1400). Secondary antibodies conjugated to Alexa® Fluor 488 or 647 (Invitrogen ...
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bioRxiv - Bioengineering 2022Quote: ... The next day samples were washed and incubated for 1h on shaker with DyLight™ 488 Donkey anti-rabbit IgG (minimal x-reactivity) Antibody (BioLegend 406404). After the incubation period ...
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bioRxiv - Developmental Biology 2019Quote: ... Cells were then incubated overnight at 4°C with primary antibody diluted in blocking solution (rabbit anti-FLAG, Sigma, 1:250 or mouse anti-Tuj1, Biolegend 1:500). After washing in PBS and incubation with secondary antibody (Alexa 647 anti-goat IgG 1:500 ...