Labshake search
Citations for BioLegend :
1051 - 1100 of 2009 citations for Rat Heat Shock Protein HSP 90 Beta HSP90AB1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Neuroscience 2023Quote: ... For further experiments viable CD45lowSiglec-H+ microglia were labeled with rat anti-ICAM1 BV711 (1:100, catalog no. 116143; BioLegend), rat anti- Ly6A/E FITC (1:100 ...
-
bioRxiv - Physiology 2023Quote: ... single cell suspension from mouse esophagi was incubated at 37°C for 30 minutes with Integrin β1 antibody (PE anti-mouse/rat CD29; 102207; Biolegend) at 1:200 dilution ...
-
bioRxiv - Immunology 2024Quote: ... and a secondary antibody goat anti-mouse antibody conjugated with APC or rat anti-mouse antibody conjugated with Alexa Fluro 700 (BioLegend). These stained cells were fixed with fixation buffer (BioLegend ...
-
bioRxiv - Physiology 2023Quote: ... the resulting single cell suspension was incubated at 37°C for 30 minutes with Integrin β1 antibody (APC anti-mouse/rat CD29; 102216; Biolegend) at 1:100 dilution ...
-
bioRxiv - Immunology 2021Quote: ... Protein tyrosine phosphorylation was detected by PY20 (Biolegend) or 4G10 (Sigma) ...
-
bioRxiv - Neuroscience 2019Quote: ... myelin basic protein (mouse monoclonal, BioLegend, Dedham, MA), PDGFRα (goat polyclonal ...
-
bioRxiv - Immunology 2022Quote: ... Biotinylated SARS-CoV-2-S1 Protein (Biolegend 793804) Anti-Biotin (409003) ...
-
bioRxiv - Immunology 2023Quote: ... RBD protein conjugated with PE-streptavidin (BioLegend, 405204) and APC-streptavidin (BioLegend ...
-
bioRxiv - Immunology 2022Quote: The following human cytokines were assessed by ELISA as previously described (10): human IFN-γ (capture MD-1 mAb; revealing biotinylated 4S.B3 mAb, Biolegend Cat#507502 and 502504, respectively), human IL-13 (capture JES10-5A2 mAb ...
-
bioRxiv - Developmental Biology 2021Quote: ... The following primary antibodies were used for immunostaining: rat anti-EpCAM-AF647 (Biolegend, clone G8.8, 1 µg ml−1, 1:250); rabbit anti-K5 (Covance ...
-
bioRxiv - Immunology 2019Quote: ... cells were permeabilized and stained with IL-10 (clone JES5-16E3, eBioscience) or isotype control (Rat IgG2b Κ Isotype Control, Biolegend). Fluorescence intensities were measured on a FACSCalibur and at least 25,000 events for lung tissue were analyzed using FlowJo.
-
bioRxiv - Immunology 2019Quote: ... from BD Sciences (San Jose, CA); Alexa Fluor® 647-conjugated anti-rat Thy1 (OX-7, #202508; RRID:AB_492884) from BioLegend (San Diego, CA). Ghost Dye™ Violet 450 was from Tonbo Biosciences (#13-0863-T100 ...
-
bioRxiv - Cell Biology 2021Quote: ... and the secondary Alexa Fluor donkey 488-conjugated anti-mouse IgG pAb (Thermo Fischer Scientific, USA) and the Alexa Fluor 667-conjugated rat anti-mouse CD31 mAb (clone MEC133, Biolegend, USA).
-
bioRxiv - Cell Biology 2020Quote: ... day-17 organoids were dissociated as described above and incubated with Alex Fluor 647-conjugated monoclonal rat anti-CD49f (BioLegend, CA) and FITC-conjugated monoclonal mouse anti-CD26 (BioLegend ...
-
bioRxiv - Animal Behavior and Cognition 2019Quote: Approximately 1×106 cells were incubated with the following antibodies in order to characterize spleen immune subpopulations: Alexa Fluor® 488-conjugated-anti rat CD3 (IF4, Biolegend), PE Cy5-conjugated-anti rat CD4 (biolegend) ...
-
bioRxiv - Genetics 2020Quote: Immunoprecipitation and immunoblotting were carried out according to standard protocols with the following antibodies: rat anti-Flag L5 antibody (BioLegend; 637301), rabbit anti-GFP antibody (Novus ...
-
bioRxiv - Cell Biology 2022Quote: ... 45-5981-82), hamster anti-mouse CD48 APC (eBioscience, 17-0481-82) and rat anti-mouse CD150 PE-Cy7 (Biolegend, 115914). To examine macrophages ...
-
bioRxiv - Cancer Biology 2022Quote: ... spun at 700 g for 3 minutes at 4°C and washed in MACS Buffer 3× before being incubated with the following antibodies: APC-conjugated rat anti-CD3 (5 μL/10×106 cells; Biolegend, 100408), PE-conjugated rat anti-CD8b (2.5 μL/10×106 cells ...
-
bioRxiv - Neuroscience 2023Quote: ... Cells were centrifuged at 1000 rpm and resuspended in flow buffer containing the primary antibodies: rat GFP (Biolegend, 338001, 1:200), guinea pig MAP2 (Synaptic Systems ...
-
bioRxiv - Immunology 2023Quote: ... PE-anti-PD-1 (clone RMP1-30, 1:20) and its isotype control PE-Rat IgG2b,κ (clone RTK4530, 1:20) were purchased from Biolegend. PE-anti-PD-L1 (clone MIH5 ...
-
bioRxiv - Immunology 2023Quote: ... Alexa Fluor 488 or PE–conjugated rat anti-mouse CD31 [platelet endothelial cell adhesion molecule 1 (PECAM1)] antibody (1:200; BioLegend, MEC13.3), Alexa Fluor 647 or FITC–conjugated rat anti-mouse CD326 (Ep-CAM ...
-
bioRxiv - Cancer Biology 2023Quote: The antibody mix for detection of MDSCs contained a rat anti-mouse CD45 (30-F11) antibody conjugated to PE (Biolegend; 103106), a rat anti-mouse Ly-6G/Ly-6C (Gr1 ...
-
bioRxiv - Cancer Biology 2023Quote: The antibody mix for CTC detection of human CTCs in animal models contained a rat anti-mouse CD45 (30-F11) antibody conjugated to PE (Biolegend; 103106) and mouse anti-human HLA conjugated to AlexaFluor 647 (Biolegend ...
-
bioRxiv - Bioengineering 2023Quote: ... Cells pre-treated with anti-mouse TfR-1 antibodies (human constant regions) were incubated with 50 nM of a second TfR-1 antibody for detection (R17, rat anti-mouse antibody; BioLegend, 113802). Cells pre-treated with anti-mouse CD98hc antibodies (human constant regions ...
-
bioRxiv - Bioengineering 2023Quote: ... Cells pre-treated with anti-mouse CD98hc antibodies (human constant regions) were incubated with 10 nM of a second CD98hc antibody for detection (RL388, rat anti-mouse antibody; BioLegend, 128202). Non-treated cells were also incubated with the TfR-1 (R17 ...
-
bioRxiv - Cancer Biology 2023Quote: The antibodies used for flow staining in this study are: rat anti-mouse/human CD11b conjugated to BV605 (clone M1/70, Biolegend, 101257), hamster anti-mouse CD3e conjugated to BV510 (clone 145-2C11 ...
-
bioRxiv - Cell Biology 2024Quote: ... all input solutions were supplemented with 1 μg/mL of PE-conjugated rat anti-mouse TNFα antibody (MP6-XT22, BioLegend, UK).
-
bioRxiv - Immunology 2024Quote: Naïve or PR8 infected B6 mice were administered daily 20µg anti-CCR2 depleting antibodies (MC-21 generously provided by Prof. Matthias Mack, Universität Regensburg) or rat IgG2b,κ isotype control (Biolegend, #400644) intraperitoneally (i.p.) ...
-
bioRxiv - Molecular Biology 2020Quote: ... or proteins (APC; Biolegend #103012 and PE; Biolegend #100206) prepared as 2 μg/ mL solutions in black-bottom 96-well plates (Greiner) ...
-
bioRxiv - Molecular Biology 2020Quote: ... or proteins (APC; Biolegend #103012 and PE; Biolegend #100206) prepared as 2 μg/ mL solutions in black-bottom 96-well plates (Greiner) ...
-
bioRxiv - Immunology 2021Quote: ... Peridinin-Chlorophyll-Protein (PerCP) anti-mouse CD19 (115531, BioLegend). SIINFEKL epitope-specific T cells were studied using APC-labelled H-2Kb/SIINFEKL pentamer (F093-84B-E ...
-
bioRxiv - Cancer Biology 2021Quote: ... Peridinin-Chlorophyll-Protein (PerCP) anti-mouse CD19 (115531, BioLegend) and PE-Cyanine 7 anti-mouse CD4 (25-0041-82 eBioscience) ...
-
bioRxiv - Immunology 2023Quote: ... All recombinant proteins and compounds were purchased from Biolegend, USA ...
-
bioRxiv - Bioengineering 2024Quote: ... Magnetic beads displaying recombinant IL-6 protein (BioLegend #570806) were prepared by washing 70 uL of Tosyl-activated M-280 magnetic dynabeads (ThermoFisher #14203) ...
-
bioRxiv - Neuroscience 2021Quote: ... The exception is that secondary labeling was accomplished using anti-rat DyLight 405 (1:5000, Novus, NBPI-72983) or anti-mouse DyLight 405 (1:1000, BioLegend, Poly-24091). Fixed cells were then imaged on a Nikon Eclipse TE2000 inverted microscope using a 60x 1.49NA oil-immersion TIRF objective and 405nm and 560nm solid lasers using a CoolSNAP HQ2 CCD camera ...
-
bioRxiv - Immunology 2020Quote: ... For investigating the formation of iBALT B cell and T cells were stained in lungs using primary antibody cocktail containing rat anti-mouse CD45R/B220 (BioLegend, Cat#103202) and Hamster anti mouse CD3 (BD ...
-
bioRxiv - Cell Biology 2023Quote: ... the pellet was resuspended in PBS and incubated for 30 min at 4 °C with rat FITC-conjugated mAb against CD11b (1:200, BioLegend, CA, USA). After incubation ...
-
bioRxiv - Immunology 2023Quote: ... PE rat IgG2a-isotype control antibody and PE mouse IgG2b-d κ isotype control antibody were purchased from BioLegend (Sandiego, CA, USA) and PE mouse IgG1-κ Isotype control antibody from BD Biosciences ...
-
bioRxiv - Immunology 2023Quote: ... mice were injected twice with 250 µg of either the isotype control IgG (Ultra-LEAFPurified Rat IgG1, λ Isotype Ctrl Antibody no. 401916, Biolegend, CA) or PC61 anti-CD25 monoclonal antibody (Ultra-LEAF Purified anti-mouse CD25 Antibody no ...
-
bioRxiv - Bioengineering 2023Quote: ... The slides were then incubated in the primary antibodies (rat anti-PECAM-1, clone 390, EMD Millipore; rabbit anti-NG-2 chondroitin sulfate, EMD Millipore; rabbit anti-Keratin-14, BioLegend) diluted 1:100 in the blocking solution overnight at 4 °C ...
-
bioRxiv - Molecular Biology 2022Quote: ... Cultures collected at different time points were subjected to TCA protein extraction (see Protein extraction by TCA) and immunoblotted with anti-Rpb1 (BioLegend 664912 clone 8WG16) or anti-Pgk1 (Abcam ab113687 clone 22C5D8 ...
-
bioRxiv - Evolutionary Biology 2022Quote: ... PE-Cy7 conjugated anti-rat CD11b/c (clone OX-42, Cat#201818), APC-Cy7 conjugated anti-rat CD4 (clone W3/25, Cat#201518) from Biolegend (San Diego, USA), and AF647-conjugated anti-rat RT1B (clone OX-6 ...
-
bioRxiv - Immunology 2019Quote: ... Cell suspensions were incubated for 10 minutes at 4°C with 10μg/ml Fc Block (purified rat anti-mouse CD16/CD32, Biolegend TruStain FcX anti-mouse) to block non-specific binding of antibodies to Fc Receptors and then incubated at 4°C for 30 minutes with anti-CD45-PE (1:300 ...
-
bioRxiv - Immunology 2019Quote: Cells were incubated for 10 minutes at 4°C with 10μg/ml of purified rat anti-mouse CD16/CD32 (Biolegend TruStain FcX anti-mouse) to block non-specific binding of antibodies to Fc Receptors and then incubated at 4°C for 30 minutes with Fixable Viability Staining e780 and fluorochrome/biotin conjugated antibodies (purchased from BD Bioscience ...
-
bioRxiv - Cell Biology 2022Quote: ... Control antibodies were biotin-conjugated rat immunoglobulin (Ig) G1 (5 μg/mL; eBioscience, San Diego, CA) and PE/Cy7-conjugated rat IgG1 (5 μg/mL; BioLegend, San Diego, CA). Flow cytometry data were collected using Fortessa LSRII (Becton Dickinson ...
-
bioRxiv - Bioengineering 2024Quote: ... Sections were then stained overnight at 4 °C in a humidified chamber with rat monoclonal anti-mouse CD68 (1:400, BioLegend, clone FA-11) and rabbit polyclonal anti-mouse IL-10 (1:25 ...
-
bioRxiv - Microbiology 2020Quote: ... in the presence of protein transport inhibitor (monensin 1000x, Biolegend). For antigen-specific study ...
-
bioRxiv - Immunology 2022Quote: ... recombinant Siglec 5-Fc chimeric protein (Biolegend, San Diego, CA) was added and incubated for 2hrs at RT ...
-
bioRxiv - Immunology 2022Quote: ... Peridinin chlorophyll protein complex (PerCP) Cy5.5-conjugated: CD4 (Biolegend, 344608), CD26 (Biolegend ...
-
bioRxiv - Immunology 2023Quote: ... in the presence of protein transport inhibitor (monensin 1000x, Biolegend). Cells were blocked with anti-CD16/CD32 antibody (TruStain FcX™ PLUS (anti-mouse CD16/32 ...