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Citations for BioLegend :
1051 - 1100 of 1595 citations for Alpha Cyclodextrin Solution 5% w v since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... single cell suspensions were incubated for 5 hours in 2μM monensin (BioLegend) to inhibit intracellular protein trafficking ...
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bioRxiv - Cell Biology 2022Quote: ... anti-CD64-PE/Dazzle594 (1:50, clone X54-5/7.1, Biolegend, 139320), anti-CD11b-PE (1:100 ...
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bioRxiv - Immunology 2023Quote: ... for 5-7h in the presence of Brefeldin A (Biolegend, Cat#420601) in a 96-well plate ...
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bioRxiv - Molecular Biology 2024Quote: ... and anti-mouse CD40 antibody (5 µg ml-1; HM40-3, Biolegend), or anti-IgM F(ab’)₂ fragment (5 µg ml-1 ...
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bioRxiv - Bioengineering 2024Quote: ... NPC pellet was resuspended in 5 mL RBC lysis buffer (BioLegend, 420301) and incubated for 5 min at 4 ℃ ...
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bioRxiv - Bioengineering 2024Quote: ... anti-mouse Ly6A/E-PE/Cy7 (5 μg/ml; BioLegend cat# 108113), anti-mouse IgG1 (20 ug/ml ...
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bioRxiv - Cancer Biology 2024Quote: ... and incubated for 5 min at room temperature with FcX block (Biolegend,) to block non-specific antibody binding ...
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bioRxiv - Cancer Biology 2024Quote: ... and Rat anti-CD4-PE (1:100, clone RM4-5, 100512, Biolegend). After staining ...
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bioRxiv - Cell Biology 2020Quote: 100µl bone marrow were blocked with 2µl Fc Receptor Blocking Solution (Human TruStain FcX™, (422301, Biolegend) for 10 minutes at room temperature ...
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bioRxiv - Neuroscience 2020Quote: ... resuspended them in staining solution containing 1:200 PE goat anti-mouse IgG secondary antibody (#405307, BioLegend) and 1:1000 Hoechst 33342 (Thermo Fisher ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells were stained with 7-Amino Actinomycin D viability staining solution (7-AAD, BioLegend, San Diego, CA). The suspensions were incubated for 45 min on ice in the dark ...
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bioRxiv - Cancer Biology 2021Quote: ... organoids were harvested in PBS and digested to a single cell solution in 1:1 Accutase (Biolegend) and TrypLE (Gibco ...
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bioRxiv - Pathology 2022Quote: ... blood was harvested at the indicated time post infection and treated with 1x RBC lysis solution (BioLegend). Murine Fc receptors were blocked using anti-mouse CD16/CD32 (FcBlock clone 2.4G2 ...
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bioRxiv - Biochemistry 2021Quote: ... Cells were resuspended in 25 μL block solution (wash plus 1 in 40 Human TruStain FcX – Biolegend) and incubated for 5 min ...
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bioRxiv - Neuroscience 2022Quote: ... Pre-centrifuged 50 μL of hashtag antibody solution containing 2 uL of TotalSeq-A Hashtag antibody (BioLegend), with a unique barcode sequence for each iPS cell line ...
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bioRxiv - Immunology 2021Quote: ... Substrate conversion was stopped after 20 minutes with 100 μL stop solution (2N H2SO4) purchased from BioLegend. Plates were washed with PBS plus 0.05% Tween20 in-between incubations ...
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bioRxiv - Cancer Biology 2022Quote: ... and cells were incubated with a blocking solution containing mouse TruStain FcX (Biolegend, San Diego, CA; #101319) before antibody staining ...
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bioRxiv - Immunology 2023Quote: ... serum samples were diluted 2-fold with assay buffer and standards were mixed with matrix solution (Biolegend) to account for additional components in the serum samples ...
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bioRxiv - Cancer Biology 2024Quote: ... Cells were first incubated with Human TruStain FcX™ (Fc Receptor Blocking Solution) (BioLegend, California, CA, USA) and TruStain FcX™ PLUS (anti-mouse CD16/32 ...
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bioRxiv - Immunology 2024Quote: ... The cells were incubated with TruStain FcX Fc Receptor Blocking Solution (anti-mouse CD16/32 antibody; BioLegend) to block nonspecific binding to Fc receptors for 10 min on ice ...
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bioRxiv - Cancer Biology 2024Quote: ... a single-cell solution of lung cells was incubated with Human TruStain FcX (Biolegend, #422302, 1:20) for 10 minutes ...
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bioRxiv - Immunology 2024Quote: ... 24 h after transfection 0.2×106 cells were fixed with Cyto-Fast Fix/Perm Solution (#750000133, BioLegend) in the dark for 15 min at room temperature and labelled with anti-GFP and anti-RFP primary antibodies in Cyto-Fast Perm/Wash solution 1X (#750000135 ...
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bioRxiv - Immunology 2021Quote: Freshly prepared cardiac single-cell suspensions from healthy and myocarditic hearts were subjected to surface staining with Annexin V (Biolegend, San Diego, CA) and propidium iodide (PI ...
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bioRxiv - Physiology 2022Quote: Apoptotic cells were analyzed by flow cytometry using the FITC Annexin V Apoptosis Detection Kit with propidium iodide (BioLegend, San Diego, CA, USA) following the manufacturer’s instructions ...
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bioRxiv - Physiology 2022Quote: ... and proceeded to Annexin V-fluorescein isothiocyanate (FITC)/PI staining according to the manufacturer’s instructions (FITC Annexin V Apoptosis Detection Kit with PI; BioLegend, San Diego, CA, USA). Cells stained with Annexin V (both Annexin V+- and Annexin V+/PI+-cells ...
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bioRxiv - Cell Biology 2022Quote: ... and the cells were resuspended in 200 μL biding buffer with 2μL 7-AAD and 2μL annexin V-FITC (Biolegend Inc, San Diego, CA, USA). The plate was incubated for 15 min in darkness and measured in the flow cytometer FC 500 (Beckman-Coulter ...
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bioRxiv - Immunology 2024Quote: ... and Propidium Iodide (PI) (1 μg/ml) according to the manufacturer’s instructions (FITC Annexin V Apoptosis Detection Kit with PI, BioLegend, San Diego, CA, USA). The staining of Annexin V requires the usage of a binding buffer for Annexin V as a staining buffer ...
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bioRxiv - Cell Biology 2021Quote: ... and the cell pellet was resuspended in 1 volume (V) CnT-PR-MSC-XF-HC before adding 3V of 1x Red Blood Cell Lysis Buffer (Biolegend, San Diego, United States). After 10 minutes of incubation at room temperature another 10V of CnT-PR-MSC-XF-HC were added and the cell suspension was centrifuged ...
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bioRxiv - Cancer Biology 2021Quote: ... Further preparation for FACS measurement was following the protocol of the BioLegend APC Annexin V Apoptosis Detection Kit and DNA-staining with DAPI Reagent (25 μg/mL) (Biolegend, San Diego, CA, USA). 20 000 cells were assayed using a flow cytometer FACSCantoII (Becton Dickinson ...
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bioRxiv - Immunology 2023Quote: Levels of murine cytokines in transplanted mice were assessed using the LEGENDplex™ Murine Inflammation panel (13-plex) in a V-bottom plate per manufacturer’s instructions (Cat. No: 740446, BioLegend, San Diego, CA, USA). LEGENDPlex data were acquired on a BD Fortessa analyzer (BD Biosciences ...
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bioRxiv - Immunology 2022Quote: Supernatants were assessed for cytokine production using the LEGENDplex™ Human Inflammation Panel 1 (13-plex) in a V-bottom plate per the manufacturer’s instructions (BioLegend, San Diego, CA, USA). Flow cytometry data were acquired on a BD Fortessa analyzer (BD Biosciences ...
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bioRxiv - Immunology 2021Quote: ... Binding of Abs was detected by developing with 50µl/well 3,3’,5,5’-tetramethylbenzidine (TMB) high sensitivity substrate solution (Biolegend, UK) and stopping with 50µl 1M sulphuric acid ...
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bioRxiv - Immunology 2022Quote: ... Cells were then incubated in 2% FCS/PBS solution containing anti-mouse CD16/32 antibody (BioLegend clone 93) for 15 minutes and subsequently stained with a combination of fluorophore-conjugated primary antibodies against mouse CD45 (BioLegend clone 30-F11) ...
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bioRxiv - Physiology 2022Quote: ... 3 106 cells were then incubated with Fc receptor blocking solution (Human Trustain FcX, Biolegend, San Diego, USA) for 5 minutes at 4°C ...
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bioRxiv - Systems Biology 2023Quote: ... before permeabilisation and nuclear staining using PBS supplemented with 2ug/mL DAPI (Biolegend, #422801, stock solution 10mg/ml), 5%FBS (Gibco ...
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bioRxiv - Immunology 2023Quote: ... was added to the wells for 1 hour at RT prior to developing with 3,3’,5,5’-tetramethylbenzidine (TMB) substrate solution (BioLegend). The reaction was stopped by adding 0.1M sulfuric acid and the optical density was determined at 450 nm using a SpectraMax iD5 spectrophotometer.
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bioRxiv - Cancer Biology 2024Quote: ... The pooled barcoded sample was stained for 10 minutes at room temperature with Fc receptor blocking solution (BioLegend) followed by 1h incubation with the antibody staining cocktail at room temprature ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were stained with goat anti parvalbumin antibodies and mounted with Ce3D™ Tissue Clearing Solution (Biolegend, 427704).
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bioRxiv - Immunology 2024Quote: Splenocytes and peripheral lymph node cells were cultured for 5 h at 37°C in complete medium (1640 RPMI + 10% FBS) plus 0.5 μM OVA257-264 and Monensin solution (1,000×; Biolegend) for cytokines detection ...
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bioRxiv - Immunology 2024Quote: ... Cells were blocked for 15 min with 10 µL of human Fc receptor blocking solution (TruStain FcXTM; BioLegend). To minimize the interaction of polymeric dyes and improve discrete fluorochrome readout ...
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bioRxiv - Immunology 2024Quote: ... Erythrocytes (RBC) were lysed for 10 min at room temperature (RT) using 1x RBC Lysis/Fixation solution (Biolegend). Samples were washed with phosphate buffered saline (PBS ...
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bioRxiv - Bioengineering 2022Quote: ... Sections were rinsed three times in IF solution and incubated with 300 nM DAPI (BioLegend, San Diego, CA) in deionized water for 15 minutes at room temperature protected from light ...
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bioRxiv - Bioengineering 2022Quote: ... The hybridoma sample was stained with a 12 μg/mL solution of TotalSeqC conjugated anti IgG1 antibody (Biolegend) and incubated for 45 minutes on Ice ...
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bioRxiv - Immunology 2022Quote: ... Cells were then incubated in 2% FCS/PBS solution containing anti-mouse CD16/32 antibody (BioLegend clone 93) for 15 minutes and subsequently stained with a combination of fluorophore-conjugated primary antibodies against mouse CD45 (BioLegend clone 30-F11) ...
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bioRxiv - Neuroscience 2022Quote: ... and incubated O/N in 50% block solution with anti-Prox1 1:500 (Biolegend, San Diego, CA; Rb), anti-ChAT 1:1K (Chemicon ...
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bioRxiv - Cancer Biology 2023Quote: ... Resuspended cells were then pooled into a single tube and incubated with Tru-Stain Fc blocking solution (Biolegend) for 10 mins at RT ...
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bioRxiv - Immunology 2023Quote: ... Cells were next treated with human 5µl/tube TruStain FcX™ (Fc receptor blocking solution, Cat# 422302, BioLegend) and incubated for 30 min at RT ...
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bioRxiv - Developmental Biology 2024Quote: ... Stained cells were washed with HBH (Hank Balanced Salt Solution (HBSS) with 0.1% bovine serum albumin (BSA) and 10mM HEPES) and stained with streptavidin-PerCP/Cy5.5 (BioLegend). Stained cells were washed ...
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bioRxiv - Cancer Biology 2024Quote: ... Then blocking solution was aspirated and the cells were incubated with phycoerythrin (PE) anti-FOLR1 antibody (908304, Biolegend) at room temperature for 1h ...
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bioRxiv - Immunology 2024Quote: ... Cells were washed 3 times with PBS containing 2% FBS before 7-AAD viability staining solution (BioLegend, #420404) was added and cells were subjected to FACS (BD FACSAria 4-laser digital flow cytometer with FACSDiva v8 software) ...