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Citations for BioLegend :
1051 - 1100 of 8128 citations for 3 Methyl 1 6 naphthyridine 2 carboximidamide hydrochloride since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2020Quote: All reagents for detection of IL-2 were purchased from Biolegend. Supernatants were collected after 16 hours of coculture of 5MCAR 58α−β− cells and M12 target cells ...
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bioRxiv - Immunology 2020Quote: ... CD19 Pacific Blue (clone H1B19, BioLegend cat#302224, 2:100 dilution); CD3 AF700 (clone OKT3 ...
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bioRxiv - Immunology 2020Quote: ... CD4 PerCP-Cy5.5 (clone OKT4, BioLegend, cat# 317428, 2:100 dilution); CD127 AF488 (clone A019D5 ...
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bioRxiv - Immunology 2021Quote: ... or 5 μg/ml anti-human TRAIL antibody (RIK-2, Biolegend). After 2 h of preincubation effector cells were added to target cells.
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bioRxiv - Systems Biology 2020Quote: ... supplemented with 2 μg/ml of anti-CD28 (clone CD28.2, Biolegend) and with 10 ng/ml of recombinant IL-2 (Peprotech ...
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bioRxiv - Immunology 2023Quote: ... followed by the addition of biotinylated anti-mouse IL-2 (BioLegend), and then streptavidin-horseradish peroxidase conjugate (SA-HRP ...
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bioRxiv - Immunology 2023Quote: ... and soluble anti-CD28 antibody (2 ug/mL, Clone: CD28.2, Biolegend) in the culture medium containing 1×Brefeldin A (Biolegend ...
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bioRxiv - Immunology 2023Quote: ... cRPMI media was supplemented with 50 ng / mL IL-2 (Biolegend), 50 ng / mL IL-15 (Biolegend).
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bioRxiv - Immunology 2023Quote: ... together with CD137 APC antibody (2:100; Biolegend, San Diego, CA). Cells were then stained on their surface for 30 min at 4°C in the dark ...
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bioRxiv - Immunology 2023Quote: ... and stained with CD63 (NVG-2) and CD206 (C068C2) from BioLegend and pro-IL-1b (NJTEN3) ...
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bioRxiv - Microbiology 2023Quote: ... PE/Cyanine5 anti-mouse IL-2 antibody (BioLegend, catalog no. 503824), PE/Cyanine7 anti-mouse IL-4 antibody (BioLegend ...
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bioRxiv - Bioengineering 2023Quote: ... and 500 IU/ml of recombinant human IL-2 (Biolegend, 589104). Stimulated T cells were co-transduced via spinoculation at 931xg ...
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bioRxiv - Immunology 2023Quote: ... cells were incubated with CD206 Ab AF647 (clone 15–2, BioLegend) and dead cell marker Zombie Violet (BioLegend) ...
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bioRxiv - Bioengineering 2023Quote: All antibodies described in Supp Table 2 were purchased from Biolegend
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bioRxiv - Immunology 2023Quote: ... as well as 2 µL of Monensin (Biolegend, San Diego, CA). Cells were cultured for 6 hours at 37°C ...
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bioRxiv - Immunology 2023Quote: ... naïve or effector CFSE-labelled OT-I (2 µM CFSE, BioLegend) were added to coverslips in complete RPMI media for 30 minutes at 37°C ...
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bioRxiv - Immunology 2024Quote: ... was purchased from Bioxcell and recombinant mouse IL-2 (carrier free, cat # 575408 from Biolegend). IL-2/anti-IL-2 complexes were prepared by mixing both reagents ...
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bioRxiv - Cancer Biology 2024Quote: ... Media were supplemented with 2 ng/ml GM-CSF (BioLegend 572902) for OCI-AML5 ...
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bioRxiv - Immunology 2023Quote: ... and 2 μl PE/Cyanine7 anti-human IgM antibody (BioLegend, 314532). Additionally ...
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bioRxiv - Developmental Biology 2022Quote: ... 2 μl Zombie Aqua dye (423101, BioLegend, San Diego, CA, USA), and 5 μl DNase I and subjected to flow cytometry ...
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bioRxiv - Genomics 2024Quote: ... 2 × 105 cells per condition were stained with CD46 antibody (BioLegend 352405 ...
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bioRxiv - Immunology 2024Quote: ... and incubated with 2 μl MojoSort Streptavidin Nanobeads (BioLegend catalog # 480015) on ice for 15 minutes ...
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bioRxiv - Immunology 2024Quote: ... followed by 2 µL/mL Zombie Aqua live/dead dye (Biolegend) for 5 min ...
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bioRxiv - Immunology 2024Quote: ... and 2 µg/ml anti-CD28 antibody (37.51) (all from BioLegend) in RPMI1640 medium (Fujifilm Wako ...
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bioRxiv - Immunology 2024Quote: ... and IL-2 secretion was measured by ELISA (Biolegend Cat # 431804).
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bioRxiv - Immunology 2024Quote: ... and mouse IL-2 ELISA kit (#431001) were purchased from Biolegend. Snap-Cell505 star (SC505 ...
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bioRxiv - Immunology 2024Quote: ... and stained with an anti-CD63-APC antibody (NVG-2, Biolegend). Fluorescence images were taken using a Nikon A1 HD25 confocal microscope.
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Intrinsically Disordered Regions Define Unique Protein Interaction Networks in CHD Family RemodelersbioRxiv - Biochemistry 2024Quote: ... and mouse erythropoietin (EPO, carrier-free, 2 U/mL, #587608, BioLegend). Cells were maintained in a humidified incubator with 5% CO2 at 37 °C ...
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bioRxiv - Bioengineering 2024Quote: Anti-human beta-2-microglobulin-FITC (BioLegend #316304, RRID:AB_492837 Clone 2M2)
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bioRxiv - Bioengineering 2024Quote: Anti-human beta-2-microglobulin-APC (BioLegend #316312, RRID:AB_10641281 Clone 2M2)
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bioRxiv - Bioengineering 2024Quote: Anti-human beta-2-microglobulin-FITC (BioLegend #316304, RRID:AB_492837 Clone 2M2)
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bioRxiv - Immunology 2022Quote: ... α-CCR10-APC (clone 6588-5) and α-TIM-3-PE-Cy7 (clone F38-2E2) from BioLegend. α-ULPB2/5/6 (mouse ...
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bioRxiv - Cancer Biology 2021Quote: ... and supplemented with murine SCF (10 ng/ml) and IL-3 (10 ng/ml) (Peprotech or BioLegend). The cells were serially passaged for ∼1 month ...
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bioRxiv - Biochemistry 2020Quote: ... Cells were blocked with PBS containing 3% BSA and stained with Alexa Fluor 647-anti-DYKDDDDK (BioLegend) at 0.5 μg/mL or with Alexa Fluor 488-streptavidin (Life Technologies ...
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bioRxiv - Molecular Biology 2021Quote: ... incubated with secondary antibody diluted in 3% BSA (Alexa Fluor 594 Goat anti-Rat IgG, BioLegend, #405422) in the dark for 1 hr at room temperature ...
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bioRxiv - Immunology 2023Quote: ... we used Ultra-LEAF™ purified anti-human TIM-3 antibody (10μg/ml, clone F38-2E2, Biolegend) or its respective isotype Ultra-LEAF™ purified mouse IgG1 antibody (10μg/ml ...
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bioRxiv - Cancer Biology 2023Quote: ... one million cells were harvested 3 days after transduction and washed twice with cell staining buffer (Biolegend). Cells were resuspended in 100μL of Cell Staining Buffer (Biolegend ...
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bioRxiv - Cell Biology 2024Quote: ... samples were permeabilized with the Intracellular Staining Perm Wash Buffer for 3 x 5 min (421002, BioLegend), followed by blocking in Superblock solution (Invitrogen ...
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bioRxiv - Immunology 2024Quote: ... ELISA plates were coated with purified antibody (3 µg/ml) overnight at 4°C (BioLegend, catalog 519202). The next day ...
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bioRxiv - Cancer Biology 2024Quote: ... Cells were allowed to proliferate for 3 days before collecting and staining with CD3e-PE (BioLegend 100308) and CD11b- PECy7 (BioLegend 101216 ...
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bioRxiv - Immunology 2024Quote: 3−5×107 PBMC were stained using a cocktail of antibodies that included CD3 (clone UCHT1, Biolegend), CD4 (clone OKT4 ...
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bioRxiv - Immunology 2022Quote: Whole PBMCs were seeded at 1-2×10^6 cells per well in 48-well plates in the presence of 200ng/ml soluble anti-CD3 (OKT3 clone, Biolegend, San Diego, CA) and 10ng/ml IL-2 (Peprotech ...
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bioRxiv - Immunology 2020Quote: ... DCs were isolated from spleens and lymph nodes of C57BL/6 mice using a MojoSort™ Mouse Pan Dendritic Cell Isolation Kit (BioLegend cat. 480097). DCs were cultured with supernatants of necrotic keratinocytes generated by an overnight culture of UV-irradiated (2 J/m2/s for 40 min ...
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bioRxiv - Immunology 2020Quote: Concentrations of IL-1β and IL-6 in cell culture supernatants or blood serum were measured using cytokine-specific ELISAs (BioLegend ELISA Max kits), according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... unpulsed splenocytes and the S-peptide-pulsed splenocytes were stained with 0.5 μM and 5 μM (respectively) of 5-(and 6-) carboxyfluorescein diacetate succinimidyl ester (CFSE; BioLegend, San Diego, CA, USA). The unpulsed and peptide-pulsed splenocytes were mixed at a 1:1 ratio (5 × 106 cells each) ...
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bioRxiv - Immunology 2021Quote: Serum samples were collected three hours after the first STING agonist injection and analyzed for inflammatory cytokines (IFN-α, IFN-ß, TNF-α and IL-6) using a LEGENDplex Mouse Inflammation Panel (BioLegend). For cell culture supernatants ...
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bioRxiv - Immunology 2023Quote: Purified and biotinylated IL-6 (504502 and 504601, respectively) and IL-10 (505002 and 504906, respectively) antibodies were purchased from Biolegend (San Diego, CA). Purified antibodies were used to coat an ELISA plate ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: Determination of the cytokine levels in the supernatants was carried out using commercial ELISA kits for human IL-6 and TNF (BioLegend, San Diego, US). ELISA was carried out according to the manufacturer’s instructions.
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bioRxiv - Immunology 2024Quote: NK cells were enriched from splenocytes from WT or Pvrig KO C57BL/6 mice by using the MojoSort™ Mouse NK Cell Isolation Kit (Biolegend, cat. 480049). Enriched NK cells were cultured and stimulated in vitro in RPMI-1640 Medium (ATCC ...
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bioRxiv - Cell Biology 2020Quote: ... Sca-1 (1:200; BioLegend), and Ter119 (1:200 ...