Labshake search
Citations for BioLegend :
951 - 992 of 992 citations for Lifeink 200 Collagen Bioink since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2021Quote: ... and then resuspended in 10% FCS in PBS (100μl/well) containing TruStain FcX™ (anti-mouse CD16/32) antibody (Biolegend; 1:200) and incubated on ice for 20mins ...
-
bioRxiv - Immunology 2020Quote: ... RAW 264.7 cells were seeded into 6-well plates at a density of 7.5 x 105 cells/well and incubated overnight with 200 U/mL of recombinant murine IFNγ (Biolegend, San Diego, CA) to promote M1-polarization and then infected with opsonized Cn for 2 h at 3:1 MOI or mock infected with PBS ...
-
Cholesterol deprivation induces TGFβ signaling to promote basal differentiation in pancreatic cancerbioRxiv - Cancer Biology 2019Quote: In order to separate cells of different origin we further stained the cell suspension with antibodies against CD45 (Biolegend #103107; 1:200), FAP (Abcam #ab28244 ...
-
bioRxiv - Cell Biology 2021Quote: ... The fixed cells were permeabilized with 0.1% Triton-X 100 for 10 min at RT and then incubated with chicken anti-vimentin (1:200, 919101, Biolegend, CA, USA) for 30 min in phosphate buffered saline (PBS ...
-
bioRxiv - Developmental Biology 2021Quote: ... Human pluripotent stem cell-derived cardiomyocytes used for IF-FISH were sorted by gating for SIRPA+ (PE-Cy7 anti-human CD172a/b, Biolegend, 1:200) and CD90- (APC anti-human CD90 (Thy1 ...
-
bioRxiv - Cell Biology 2020Quote: ... a 6-colour flow cytometry panel was performed on a Bio-Rad ZE5 Cell Analyzer using antibodies for CD56 (Clone HCD56, Brilliant Violet 605, BioLegend, 1:200), CD3 (Clone SK7 ...
-
bioRxiv - Neuroscience 2022Quote: ... and 0.3% PBST for 3 days at 4°C on rotating incubator (NFH, 1:500, Abcam, ab4680; Tubulin β-III, 1:200, Biolegend, 801201). Whole muscles were washed in 0.3% PBST for 5 hours then incubated in secondary antibodies for 48h at 4°C on rotating incubator (AlexaFluor 568 ...
-
bioRxiv - Cell Biology 2023Quote: ... the pellet was resuspended in PBS and incubated for 30 min at 4 °C with rat FITC-conjugated mAb against CD11b (1:200, BioLegend, CA, USA). After incubation ...
-
bioRxiv - Cancer Biology 2023Quote: ... Primary antibodies used and the dilutions were as follows: for neurons: anti-Tubulin-βIII Rabbit mAb (1:200, BioLegend, 802001, clone TUJ1); anti-Synapsin-1 Mouse mAb (1:200 ...
-
bioRxiv - Immunology 2023Quote: ... Cells were pelleted down at 1,500 rpm at 4°C for 5 min and cell pellets were resuspended in 100 uL FACS buffer (PBS + 4% FBS +0.5 mM EDTA) containing Fc block (BioLegend, 101302, 1:200). After 15 min incubation at 4°C ...
-
bioRxiv - Biochemistry 2023Quote: ... The fixed cells were permeabilized with 0.1% Triton-X 100 for 10 min at RT and then incubated with chicken anti-vimentin (1:200, 919101, Biolegend, CA, USA) for 30 min in phosphate buffered saline (PBS ...
-
bioRxiv - Immunology 2022Quote: ... CD3ε clone 145-2C11; Ly-6G/Ly-6C clone RB6-8C5; CD11b clone M1/70; CD45R/B220 clone RA3-6B2; TER-119, Biolegend, 1/200). The c-kit− fraction was stained with antibodies against CD11b (PercPCy5.5 ...
-
bioRxiv - Immunology 2021Quote: ... cells were stained in PBS at 4°C for 30 minutes with the fixable viability dye eFluor 780 (1:1000, 65-0865-14 eBioscience) and a combination of the following antibodies (1:200, all from BioLegend unless stated otherwise): anti-mouse CD45 BV650 (30-F11 ...
-
bioRxiv - Immunology 2019Quote: ... The stain solution contained a 1:200 dilution of an antibody against H-2Kb bound to SIINFEKL conjugated to PE (#141603, Biolegend, San Diego, CA) to detect SIINFEKL peptide being presented at the cell surface ...
-
bioRxiv - Cancer Biology 2021Quote: ... Cells were stained for 30 min at +4°C with APC or PE-conjugated anti-CD133 antibodies (diluted at 1/200 or 1/400 respectively; Biolegend, San Diego, USA) or corresponding control Immunoglobulin G1 (IgG1 ...
-
bioRxiv - Immunology 2022Quote: ... extracellular staining was done in ice-cold buffer (phenol red–free DMEM, 2% FBS) for 30 min using antibodies against CD45 (30-F11; BioLegend, dilution 1:200), CD4 (RM4-5 ...
-
bioRxiv - Immunology 2022Quote: ... CD11bhi, F4/80hi) were isolated by FACS using antibodies against CD11b (eBioscience, #11-0112, 1:1600) and F4/80 (Biolegend, #123114, 1:200), and total RNA was extracted using RNeasy Plus Mini Kit (Qiagen) ...
-
bioRxiv - Immunology 2019Quote: ... cells were washed with 200 μl FACS Buffer and blocked in 100 μl Fc block (cat. no. 101319, TruStain FcX, clone 93, BioLegend, diluted 1:100) in FACS Buffer for 10 min on ice and washed ...
-
bioRxiv - Neuroscience 2019Quote: ... The triangularis sterni muscle was dissected and incubated overnight (4°C) in anti-βIII-tubulin antibody conjugated to Alexa488 (BioLegend 801203; 1:200) and Alexa594-conjugated α-bungarotoxin (Invitrogen B13423 ...
-
bioRxiv - Immunology 2021Quote: ... Cells with successful PD-L1 deletion were isolated by two rounds of FACS sorting after O/N stimulation with 100 ng/ml IFNγ and staining with PD-L1-PE/Cy7 (clone 10F.9G2, Biolegend 124314, 1:200). Cells retaining PD-L1 expression were isolated simultaneously and served as controls.
-
bioRxiv - Cell Biology 2022Quote: ... and the cells were resuspended in 200 μL biding buffer with 2μL 7-AAD and 2μL annexin V-FITC (Biolegend Inc, San Diego, CA, USA). The plate was incubated for 15 min in darkness and measured in the flow cytometer FC 500 (Beckman-Coulter ...
-
bioRxiv - Cancer Biology 2022Quote: ... Cells were then resuspended in 50 µl of flow buffer (1x PBS, 2% FBS, 10mM EDTA, 25mM HEPES) containing anti-CD16/CD32 antibody for Fc receptor blockade (Biolegend, 101320, 1:200) and incubated on ice for 5 minutes ...
-
bioRxiv - Immunology 2022Quote: ... CD44 – PE-Cyanine7 (eBiosciences, # 25-0441-81; 1:600), CD4 – APC (eBiosciences, #17-0042-83; 1:200) and CD8a – APC-Cyanine7 (BioLegend, #100714; 1:400) combined with AZDye 488 alkyne/azide ...
-
bioRxiv - Microbiology 2023Quote: 2 × 106 single cell suspensions prepared from the lung or spleen were resuspended in 200 ul of PBS with 2% fetal bovine serum and blocked with TruStain fcX (BioLegend, San Diego, CA). T cell subsets were identified with antibodies against CD45 (clone 30-F11 ...
-
bioRxiv - Cell Biology 2020Quote: ... Surface marker staining was performed for 30 min on ice using CD34-eFluor 660 (eBioscience, clone RAM34, 1:100) and ITGA6-pacific blue (Biolegend, clone GoH3, 1:200). Cells were washed with PBS and stained with annexin V-AF488 (ThermoFisher Scientific ...
-
bioRxiv - Microbiology 2019Quote: ... Cells were permeabilized with ice-cold methanol at −20°C for at least 20 min and incubated with anti-Pol II antibody (Biolegend, 8WG16 at 1:200), anti-PABPC antibody (Abcam ab21060 at 1:200 for mouse cells and Santa Cruz sc32318 at 1:25 for human cells ...
-
bioRxiv - Cell Biology 2021Quote: ... The supernatant was discarded and the cell pellets were resuspended in PBS containing Zombie Violet dye (Biolegend; 1:200 in PBS; 100μl per well) and incubated at room temperature ...
-
bioRxiv - Genomics 2020Quote: ... cells were resuspended in PBS complemented with 2% fetal bovine serum and stained with FITC anti-human CD45 antibody (BioLegend #304006CD45, 1:200 dilution) and PE anti-human EPCAM antibody (Miltenyi Biotech #130-113-264 ...
-
bioRxiv - Neuroscience 2022Quote: ... APC-Cy7 rat anti-mCD45 (1:200, Becton Dickinson, Franklin Lakes, NJ, USA) and APC-Cy7 anti-mTer119 (1:100, Biolegend, San Diego, CA, USA). In order to sort neural stem and precursor cells ...
-
bioRxiv - Immunology 2022Quote: ... 4 °C) cells were stained with the following conjugated antibodies for 20 min at RT in the dark: CD3-APC (Biolegend, clone REA613, 1:200), CD11b-APC-Cy7 (Biolegend ...
-
bioRxiv - Cancer Biology 2023Quote: ... IV PBS + IP anti-mouse PD-L1 mAb (200 μg/mouse; Ultra-LEAF™ purified anti-mouse PD-L1 Ab, clone 10F.9G2; BioLegend, San Diego, CA), IV iRGD + IP anti-mouse PD-L1 mAb ...
-
bioRxiv - Neuroscience 2024Quote: ... flow cytometry staining master mix was prepared containing 1:200 dilutions of PE anti-mouse/human CD11b Antibody (BioLegend, Cat#:101208, clone M1/70), APC anti-mouse CX3CR1 (Antibody BioLegend ...
-
bioRxiv - Cell Biology 2024Quote: ... The following primary antibodies were used in this study: anti-tubulin beta-3 conjugated to Alexa Fluor 488 (BioLegend; AB_2562669; mouse IgG2a, 1:200), Alexa Fluor 555 (BD PharMingen ...
-
bioRxiv - Immunology 2023Quote: ... pDCs were gated for flow cytometry with 1:20 dilution commercial mouse anti-human BDCA-2-Alexa488 (stock 200 ug/mL, BioLegend, San Diego, CA, USA) and 1:20 dilution mouse anti-human CD123 (IL-3)-PE (stock 200 ug/mL ...
-
bioRxiv - Cancer Biology 2023Quote: ... peripheral blood and spleens were harvested from each mouse and the infiltrated primary cells were identified using flow cytometry by staining for human CD45 antibody (BioLegend; BLG-368522; 1:200).
-
bioRxiv - Cancer Biology 2021Quote: ... Cells were fixed with 2% neutral-buffered formalin diluted in FACS buffer following addition of Zombie Yellow fixable viability dye (BioLegend #423103, 1:200 in PBS).
-
bioRxiv - Neuroscience 2020Quote: ... the neurons were fixed and stained as previously described38 using primary antibodies for β-3-Tubulin (TUJ1; 1:200; BioLegend, San Diego, CA, USA; 801201) and TH (1:300 ...
-
bioRxiv - Cell Biology 2021Quote: ... The supernatant was discarded and the cell pellets were resuspended in PBS containing Zombie NIR™ dye (Biolegend; 1:200 in PBS; 100μl per well) and incubated at room temperature ...
-
bioRxiv - Cell Biology 2021Quote: ... The supernatant was discarded and the cell pellets were resuspended in PBS containing Zombie NIR™ dye (Biolegend; 1:200 in PBS; 100μl per well) and incubated at room temperature ...
-
bioRxiv - Cancer Biology 2022Quote: Epithelial crypt cells from the ileum of Ki67-RFP ApccKO/cKO Villin-CreERT2 mice and microadenomas from Rosa26-tdTomato ApccKO/cKO Lgr5-EGFP-CreERT2 mice were stained with Pacific Blue™ (PB) anti-CD45 antibody (#103126, BioLegend, USA; dilution 1:200), PB-conjugated anti-CD31 (#102422 ...
-
bioRxiv - Cancer Biology 2023Quote: ... The bone marrow was harvested from each mouse and the infiltrated CCRF cells were identified by staining for a human CD45 antibody (Biolegend; AB_2687375; BLG-368522; 1:200).
-
bioRxiv - Pharmacology and Toxicology 2019Quote: Immunofluorescence staining was performed in 96 well plates on seminiferous tubules with primary antibodies major histocompatibility complex class II [MHC Class II RT1D (BioLegend, Clone OX-17, 205401, 1:200)] ...