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Citations for BioLegend :
951 - 1000 of 9012 citations for 6H Pyrazolo 3 4 b pyridin 6 one 3 cyclobutyl 1 2 4 5 tetrahydro 4 methyl 9CI since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2019Quote: ... Three µl (3 µg) anti-HA.11 epitope tag antibody (BioLegend, 901501) was added to each of the extracts and all samples were incubated on a rotor at 4°C for 4 hrs ...
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bioRxiv - Immunology 2019Quote: ... anti-mouse CD206 (cat. # 141708, clone C068C2, APC, 3 μl, Biolegend, CA), anti-human CD80 (cat ...
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bioRxiv - Physiology 2019Quote: ... and anti-LAG-3 (C9B7W) (all from Biolegend, San Diego, CA, USA). Anti-IFN-γ (XMG1.2) ...
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bioRxiv - Immunology 2021Quote: ... Cells were washed every three days and supplemented with rmIL-3 (BioLegend), and BME (Gibco) ...
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bioRxiv - Pathology 2022Quote: ... Cells were then washed 3 times with cell staining buffer (#420201, BioLegend) followed by resuspension of the cells in DAPI containing FACS buffer ...
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bioRxiv - Cancer Biology 2022Quote: ... 10 ng/mL human recombinant IL-3 (BioLegend, San Diego, CA, USA) and antibiotics ...
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bioRxiv - Immunology 2021Quote: ... Bcl-6 FITC (Biolegend 358513, 1:100), and Glut-1 AlexaFluor 647 (Abcam ab115730 ...
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bioRxiv - Microbiology 2021Quote: ... 1 U/ml penicillin/streptomycin) with 3 ng/ml recombinant mouse GM-CSF (rGM-CSF; R&D Systems or BioLegend)) at a concentration of 2.5 x 105 cells/ml in 150 x 15 mm petri plates at 37°C with 5% CO2 ...
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bioRxiv - Microbiology 2019Quote: ... The cells were then washed with ice-cold FACS Buffer (1x PBS + 3% FBS) before staining with mouse anti-HA (diluted 1:200, Biolegend) for 30 minutes on ice ...
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bioRxiv - Neuroscience 2020Quote: ... Cells were blocked in 10% BSA in PBS for 1 h at room temperature and then incubated with a MAP2 antibody diluted in 3% BSA (MAP2 chicken, 1:10000, Biolegend) for 60 min at room temperature ...
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bioRxiv - Neuroscience 2022Quote: ... rabbit) at 1:100 dilution (for immunodepletion) or 3 μg/ml of each 4G8 and 6E10 anti-Aβ antibodies (Biolegend) (for brain-derived Aβ enrichment ...
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bioRxiv - Immunology 2023Quote: ... Sip-T or single cell suspensions of tumor-infiltrating lymphocytes (TILs) were resuspended in PBS as 1-3×106 cells/ml and stained with fixable Zombie Aqua viability dye (BioLegend) as per manufacturer’s recommendation ...
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bioRxiv - Cell Biology 2023Quote: ... samples were stained for proliferation marker Ki-67 Alexa Fluor 647 (1:250 dilution in 3% BSA, Cat. No. 652407; BioLegend) for 1 hour and nuclei counterstain bisbenzimide (1:1000 dilution in 1X dPBS ...
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bioRxiv - Immunology 2023Quote: ... naïve CD8+ T cells were cultured in RPMI (10% FCS) and stimulated with plate-bound mCD3 mAbs (3 µg ml-1, clone 145-2C11, Biolegend), soluble mCD28 (0.5 µg ml-1 ...
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bioRxiv - Cancer Biology 2021Quote: ... HLA-A,B,C (FITC, Biolegend, #311404, 1:100 dilution), mouse IFNGR1 (PE ...
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bioRxiv - Cancer Biology 2024Quote: ... and Granzyme-B-PE/Dazzle 594 (372215, Biolegend, 1:100) were used to stain healthy donor T cells in suppression assays ...
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bioRxiv - Cancer Biology 2022Quote: ... TGFß (1 ng/ml; cat#763102) and IL-2 (5 ng/ml, cat# 575402; All Biolegend) were added along with T cell media (RPMI ...
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bioRxiv - Immunology 2022Quote: ... and 5 ng/mL IL-2 (Biolegend 575404) at 1×107 cells/mL for 72 hrs ...
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bioRxiv - Immunology 2022Quote: ... Every 10^6 B cells in 100 μL were then stained with 2.5 μL FITC anti-human CD19 antibody (BioLegend, 392508), 2.5 μL FITC anti-human CD20 antibody (BioLegend ...
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bioRxiv - Immunology 2023Quote: ... from uninfected or MCMV-infected WT C57BL/6 mice were sorted as described and stained with barcoded antibodies (Total-Seq B, BioLegend). After hash-staining ...
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bioRxiv - Neuroscience 2023Quote: The single-cell suspension was stained at 4°C for 15 minutes with antibodies recognizing lymphocytes and endothelial cells (anti-CD45/CD31; BioLegend Way, San Diego, CA, USA) and labeling live cells (CellTrace calcein green ...
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bioRxiv - Genomics 2024Quote: ... Antibodies were added to stain surface markers for 30 min at 4°C (CD3 PE, Life Technologies; CD8 FITC, Life Technologies; CD11b AF700, Life Technologies CD19 PerCP-Cy5.5, BioLegend; C33 BV605, BioLegend; CD56 BV711, BD). After 30min ...
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bioRxiv - Cancer Biology 2020Quote: ... VDR overexpressing SKOV-3 and ECC-1 cells were processed and stained with PDL1-BV605 antibody (Biolegend Inc, Catalog number:124321). VDR expressing SKOV-3 and ECC-1 cells ...
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bioRxiv - Biochemistry 2020Quote: ... Cells were blocked with PBS containing 3% BSA and stained with either APC-conjugate anti-human CD99 antibody clone 3B2/TA8 (1:20 dilution, BioLegend 341307), PE anti-human CD166 clone 3A6 (1:20 dilution ...
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bioRxiv - Genetics 2023Quote: ... Recombinant Anti-AKT1 + AKT2 + AKT3 (phospho S472 + S473 + S474, Purified anti-Tubulin β-3 (TUBB3) Antibody 1:1000 (Biolegend, # PRB-435P), anti-GAPDH antibody 1:1000 (Sigma Aldrich ...
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bioRxiv - Neuroscience 2024Quote: ... 0.3% triton in PBS) for 3 hours then incubated with Alexa Fluor 594 anti-β-amyloid (Clone 6E10; 1:1,000; BioLegend; 803019), Olig2 (1:500 ...
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bioRxiv - Immunology 2019Quote: ... anti-mouse CD107b (Mac-3) and anti-mouse TNF (MP6-XT22) (all Biolegend). Live-Dead Fixable Green and Live-Dead Fixable Aqua Dead Cell stains was purchased from Thermofisher (Boston ...
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bioRxiv - Bioengineering 2021Quote: ... and 20 ng/mL recombinant human IL-3 (BioLegend; San Diego, CA, USA) to determine cellular distribution ...
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bioRxiv - Immunology 2020Quote: ... and anti-IFNγ Alexa 647 (clone 4S.B3; 3 μL; cat# 502516) from Biolegend; anti-CD3 BV605 (clone SP34-2 ...
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bioRxiv - Immunology 2022Quote: ... the sera were applied to 3 ug/ml goat anti-rat IgG (BioLegend), or 1% BSA (ThermoFisher ...
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bioRxiv - Immunology 2019Quote: ... anti-mouse CD80 (cat. # 104707, clone 16-10A1, PE, 3 μl, Biolegend, CA), anti-mouse CD206 (cat ...
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bioRxiv - Immunology 2021Quote: ... mice were injected with 3 µg anti-CD45-BV510 (clone 30-F11, Biolegend) intravenously and were euthanized three minutes later with an overdose of inhaled isoflurane ...
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bioRxiv - Bioengineering 2022Quote: CPCs and MSCs at passage 3 were suspended in cell staining buffer (Biolegend) (106 cells/100μl) ...
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bioRxiv - Immunology 2022Quote: ... and a soluble mAb anti-CD3 (clone OKT-3, 140 ng/mL; BioLegend), were added to the medium at day 0 ...
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bioRxiv - Microbiology 2024Quote: ... Staining was done with 3-amino-9-ethylcarbazole (AEC) (BioLegend, Amsterdam, The Netherlands) as HRP substrate ...
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bioRxiv - Immunology 2024Quote: ... and 3 IFNγRKO tumours were labelled with TotalSeq(TM)-C0302 (Biolegend, Cat. 155863). Live cells stained with Zombie NIR and CD45 antibody were sorted based on expression of CD45 using a BD FACSAria™ II ...
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bioRxiv - Genetics 2023Quote: ... cells were stained by Anti-TUBB3 (tubline beta 3; Biolegend Inc, Cat# 801202) in a blocking solution overnight at 4° ...
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bioRxiv - Immunology 2020Quote: Supernatants of stimulated T cells were harvested on day 6 of culture and analyzed using the LEGENDplex™ human Thelper 1/2/9/17 multiplex kit (BioLegend). Beads were acquired on a BD Canto II (BD Bioscience ...
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bioRxiv - Cell Biology 2022Quote: ... and IL-6 (MP5-20F3, Biolegend; 1:100) antibody ...
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bioRxiv - Bioengineering 2023Quote: ... CD66b-BV421 (1:50, clone 6/40c, Biolegend), CD127-PerCP-Cy5.5 (1:20 ...
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bioRxiv - Developmental Biology 2021Quote: ... The cells were then incubated for one hour at 4°C in the dark with a mix of rat anti-mouse CD41 (labelled with Phycoerythrin, PE) (BioLegend – 133906; 0.25 µg per million cells), rat anti-mouse CD31 (labelled with AF647 ...
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bioRxiv - Developmental Biology 2021Quote: ... then stained for one hour at 4°C in the dark with a mix of rat anti-mouse CD117/c-kit (labelled with PE) (BioLegend – 105808; 0.4 µg per million cells), rat anti-mouse CD34 (labelled with AF700 ...
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bioRxiv - Neuroscience 2023Quote: ... ectodermal paired box 6 and SRY-box transcription factor 2 (PAX6/SOX2, Biolegend, San Diego ...
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bioRxiv - Developmental Biology 2020Quote: ... anti-human CD172a/b (SIRPα/β) (Biolegend; clone SE5A5; 1:200) anti-human CD13 (Biolegend ...
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bioRxiv - Cell Biology 2021Quote: PE-Cy7 anti-human CD172a/b (SIRPalpha/beta, Biolegend, 1:200), PE anti-human CD90 (Biolegend ...
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bioRxiv - Bioengineering 2023Quote: ... HLA-A/B/C-BV605 (1:20, clone W6/32, Biolegend). We used the following anti-mouse antibodies ...
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bioRxiv - Immunology 2023Quote: Antigen-specific B cell subsets were detected using biotinylated SARS-CoV-2 RBD protein (793906; Biolegend) in combination with streptavidin (SAv)-fluorophore conjugates as described [7] ...
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The T-cell niche tunes immune function through modulation of the cytoskeleton and TCR-antigen forcesbioRxiv - Bioengineering 2024Quote: ... and granzyme B (anti-Granzyme B Pacific Blue, Biolegend, # 515407) production using flow cytometry (% positive T-cells) ...
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bioRxiv - Microbiology 2023Quote: ... Tissue was rinsed 3 times in 1X PBS and then incubated with 1:5000 dilution rabbit polyclonal anti-Keratin-14 antibody (Biolegend, Clone Poly19053) for 1 hour and then detected with 1:10000 dilution goat anti-Rabbit IgG Secondary Antibody conjugated with Alexa Fluor™ 488 (ThermoFisher Scientific ...
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bioRxiv - Immunology 2023Quote: ... then were resuspended in FACS (PBS + 3% FBS + 1mM EDTA + 10mM HEPES) supplemented with TruStain FcX (1:50; BioLegend; San Diego, CA) and TruStain Monocyte Blocker (1:20 ...