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Citations for BioLegend :
951 - 1000 of 8186 citations for 1 2 4 4 4 Pentachloropentafluorobutane since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... 2 % FCS (1 to 5 x107 cells/500 μl) and incubated with the following mAbs: CD45 AF700 (Biolegend, clone HI30), CD34 PB (Biolegend ...
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bioRxiv - Immunology 2023Quote: Supernatants from stimulated T cells were harvested on day 2 and day 6 depending on peak of cytokine production and analyzed using the LEGENDplexTM human Thelper 1/2/9/17 multiplex kit (BioLegend). Beads were acquired on a BD Canto II (BD Bioscience ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were then diluted with 50 μL PBS + 2% FBS and FITC anti-CD45 (BioLegend, 304005; 1:1,000 final dilution) and incubated for 20 min on ice in the dark ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Cells were centrifuged at 500× g at 4 °C and resuspended in 1/3 strength artificial seawater containing 2 μg/mL calcein AM (Enzo) and 100 nM sytox blue (BioLegend) to monitor viability ...
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bioRxiv - Immunology 2023Quote: ... 0.5-1×106 bone marrow cells were stained with the following surface antibodies (2 panels) against: CD14 (Biolegend, M5E2, AF700), HLA-DR (Biolegend ...
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bioRxiv - Cancer Biology 2024Quote: ... Surface staining of the Macrophage panel consisted of the following primary antibodies diluted in FACS buffer (2% FBS in DPBS) with anti-mouse CD45-Brilliant Violet 650 (1:200, 103151, Biolegend), anti-mouse F4/80-Brilliant Violet 785 (1:200 ...
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bioRxiv - Microbiology 2024Quote: ... LAMP-1 was stained as described previously for LAMP-2 but using a rat-IgG anti-mouse LAMP-1 and anti-rat-IgG-AlexaFluor647 (Biolegend) [56] ...
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bioRxiv - Immunology 2024Quote: ... and fluorochrome-conjugated anti-CD86 and anti-CD32 antibodies in FACS buffer (1%BSA and 2 mM EDTA in PBS) supplemented with True Stain Monocyte Blocker solution (Biolegend) to prevent aspecific binding of fluorochrome-conjugated antibodies to myeloid cells ...
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bioRxiv - Molecular Biology 2024Quote: ... The cells were washed with PBS containing 2 mM EDTA and stained with antibodies against CD59 (1:100, anti-human CD59 PE, Biolegend) and C3c (1:25 ...
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bioRxiv - Immunology 2023Quote: ... IL-2 secretion was evaluated with the IL-2 assay ELISA kit (Cat#431801, Biolegend). The absorbance value at 450 nm/570 nm was detected by the microplate reader ...
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bioRxiv - Immunology 2024Quote: ... the amount of secreted IL-2 was quantified using Human IL-2 ELISA kit (Biolegend).
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bioRxiv - Immunology 2020Quote: ... were added to the tumor cells and incubated for 2 hours with CD20-TCB (200 ng/ml) and +/- αLFA1 antibody (Clone: TS 1/18, 10 μg/ml, BioLegend, 302116). Cells were then gently washed ...
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bioRxiv - Immunology 2020Quote: Supernatants of stimulated T cells were harvested on day 6 of culture and analyzed using the LEGENDplex™ human Thelper 1/2/9/17 multiplex kit (BioLegend). Beads were acquired on a BD Canto II (BD Bioscience ...
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bioRxiv - Immunology 2021Quote: ... Approximately 2 million cells per experimental condition were stained with 1 μg of TotalSeq™ B0301-B0308 anti-mouse Hashtag antibodies (BioLegend) according to the manufacturer’s instructions and pooled together at equal cell numbers ...
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bioRxiv - Immunology 2024Quote: ... Cells were then washed with FACS buffer (PBS+ 2% (v/v) FBS with 1mM EDTA) and incubated with a 1:500 dilution of TruStain FCX (Biolegend, 101320) for 5 minutes ...
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bioRxiv - Cell Biology 2024Quote: ... and the resulting suspension was plated at 1-2×106 cells/ml in complete medium containing 20% FCS and 20 ng/ml M-CSF (BioLegend, 574804). Cells were incubated for 6 days with a medium replacement at day 3 ...
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bioRxiv - Bioengineering 2023Quote: THP-1 cells were incubated with 2 µg/ml of anti-CD44 antibodies (clone: C44Mab-5; Biolegend, San Diego, CA, USA) for 20 minutes under standard cell culture conditions (95% humidity and 5% CO2 at 37°C ...
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bioRxiv - Immunology 2023Quote: ... we incubated sequential de-paraffinized sections with anti-2’,3’-cyclic-nucleotide 3’-phosphodiesterase (CNPase) (mouse CNPase: 1:200 dilution; Biolegend, Inc.), anti-CD3 (rabbit ...
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bioRxiv - Immunology 2023Quote: ... For the memory B cell EliSpot, PBMC were stimulated with 1 µg/ml R848 (SigmaAldrich, SML0196) and 10 ng/ml recombinant human IL-2 (Biolegend, 791904) for 5 days before harvesting and plating of 50-100.000 cells/well ...
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bioRxiv - Neuroscience 2024Quote: ... We then blocked the brains in 5 % goat serum for 2 h and stained with mouse anti-Aβ42 (6E10) (1:500, BioLegend, CA, USA) in PBST on a shaker at 4 °C overnight ...
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bioRxiv - Immunology 2024Quote: ... and one of the hashtag antibodies (TotalSeq™-C0251 anti-human Hashtag 1 Antibody, LNH-94 2M2, BioLegend #394661; TotalSeq™-C0252 anti-human Hashtag 2 Antibody, LNH-94 2M2, BioLegend #394663 ...
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bioRxiv - Immunology 2024Quote: ... lymphocytes or purified NK cells were resuspended in FACS buffer (PBS supplemented with 2 % FBS) and anti-CD3-PE (1:200, BioLegend, #300308) and anti-CD56-BV421 (1:100 ...
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bioRxiv - Immunology 2024Quote: ... labeled RMA-S cells at a 2:1 Effector to Target ratio in RPMI supplemented with 10ng/mL rIL-15 (Biolegend 566302). Co-cultures were incubated at 37° degrees for 5 hours ...
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bioRxiv - Cancer Biology 2024Quote: ... cells were incubated for 2 h with Alexa Fluor® 647 anti-Tubulin-α antibody (1:250 dilution, clone 10D8, BioLegend) prepared in the permeabilization/blocking buffer ...
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bioRxiv - Developmental Biology 2024Quote: ... Cell pellets were collected by centrifugation and resuspended in 100µl PBS/2% serum supplemented with Alexa Fluor 647 anti-CD15 (SSEA-1) (Biolegend, cat. 125608) and Phycoerythrin (PE ...
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bioRxiv - Immunology 2021Quote: ... anti-CD138 (clone 281-2, Biolegend). Specific conjugates are indicated within Figures ...
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bioRxiv - Immunology 2021Quote: ... and Thy1/2 (Biolegend, 30-H12) conjugated to APC-Cy7 each at a concentration of 0.25 μg per 1 x 106 cells to remove dendritic cells ...
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bioRxiv - Immunology 2021Quote: ... IL-2 (20 ng/ml; BioLegend), IL-4 (20 ng/ml ...
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bioRxiv - Immunology 2022Quote: ... IL-2 (clone: JES6-5H4; BioLegend) and IL-17A (clone ...
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bioRxiv - Immunology 2022Quote: ... and Monensin solution (2 μM) (BioLegend). Cells were then stained with one of four antibody panels (Supplementary Table 1 ...
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bioRxiv - Immunology 2020Quote: ... anti-IL-2-PE-Cy7 (Biolegend) and anti-IL-4-PE (Biolegend) ...
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bioRxiv - Bioengineering 2021Quote: ... αCD4 mAb (clone RM4-2, Biolegend), anti-Camelid VHH antibody (clone 96A3F5 ...
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bioRxiv - Bioengineering 2021Quote: ... αCD4 mAb (clone RM4-2, Biolegend), αCD11b mAb (clone M1/70 ...
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bioRxiv - Immunology 2020Quote: ... α-BCL-2 (BCL/10C4, 633510, Biolegend), α-BIM (K.912.7 ...
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bioRxiv - Immunology 2020Quote: Panel 2: CD11b-BV421 (Biolegend, 301323), CD274-PE (Biolegend ...
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bioRxiv - Immunology 2020Quote: ... anti-SDC1-PE (281-2, BioLegend), and anti-FR4-PE Cy7 (ebio12A5 ...
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bioRxiv - Immunology 2021Quote: ... IgD PE-Cy7 (IA6-2, BioLegend) Zombie NIR (BioLegend ...
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bioRxiv - Immunology 2021Quote: ... IgD PE-Cy5 (IA6-2, BioLegend), CD14 PerCP (HCD14 ...
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bioRxiv - Immunology 2022Quote: ... IL-2 BV421 (Biolegend, cat.503826), IL-4 PE (Biolegend ...
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bioRxiv - Immunology 2022Quote: ... α-CD8b.2 APC (53-5.8, BioLegend) and α-FOXO1 (C29H4 ...
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bioRxiv - Immunology 2024Quote: ... CD138-BV650 (Biolegend, Clone: 281-2), CD31-APCCy7 (Biolegend ...
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bioRxiv - Immunology 2024Quote: ... and monensin (2 mM) (Biolegend, 420701) in iron free media at 37°C/5% CO2 for 5h prior to staining.
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bioRxiv - Bioengineering 2024Quote: ... α-total ERK1/2 (Biolegend 686902) 5000x ...
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bioRxiv - Immunology 2023Quote: ... IL-2-PE (JES6-5H4; Biolegend), TNFα-Fitc (MP6-XT22) ...
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bioRxiv - Neuroscience 2024Quote: ... CD202b (TIE-2) (BioLegend, Cat#334204), CD146 (BioLeg-end ...
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bioRxiv - Immunology 2024Quote: ... IL-2-Alexa700 [all from BioLegend] ...
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bioRxiv - Cancer Biology 2024Quote: ... anti-mouse H-2 (Biolegend, 125506), anti-mouse I-A/I-E (Biolegend ...
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bioRxiv - Cancer Biology 2023Quote: ... IL-2 and TNFα kits (BioLegend) and the experiments were done according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2023Quote: ... 200 IU/ml IL-2 (Biolegend), 12.5% FBS ...
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bioRxiv - Immunology 2023Quote: ... anti-CD49d (Biolegend, clone: R1-2), anti-NK1.1 (Biolegend ...