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Citations for BioLegend :
51 - 100 of 3269 citations for VEGF C Human 196a.a HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... Phycoerythrin (PE) anti-His tag antibody (BioLegend 362603) was added to the cell suspension at a dilution of 1:20 and incubated for 30 minutes on ice in the dark ...
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bioRxiv - Molecular Biology 2023Quote: ... and mouse anti-His (1:2500, 362601, BioLegend) were diluted in 5% BSA in 1× PBS + 0.2% Tween20 for blotting ...
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bioRxiv - Cell Biology 2019Quote: ... cells were then incubated for 10 minutes at 4°C in human Fc Blocking reagent (FcX, BioLegend). Cells were incubated at 4°C for a further 30 minutes with 0.5µg of TotalSeq™-A (Biolegend ...
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bioRxiv - Cell Biology 2021Quote: ... cells were then incubated for 10 minutes at 4°C in human Fc Blocking reagent (FcX, BioLegend). Cells were incubated at 4°C for a further 30 minutes with 0.5μg of TotalSeq-A (Biolegend ...
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bioRxiv - Cell Biology 2021Quote: ... were blocked with 5ul Human TruStain FcX™ Blocking Reagent for 10 minutes at 4°C (BioLegend). 1ug of each TotalSeq™-A antibody was added to the cell suspension ...
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bioRxiv - Microbiology 2020Quote: ... cells were stained for 30 min at 4°C with FITC-conjugated anti-human CD3 antibody (Biolegend), followed by fixation and permeabilization of cells with BD Cytofix/Cytoperm™ (BD Bioscience) ...
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bioRxiv - Immunology 2021Quote: ... PE anti-human HLA-A,B,C (clone W6/32; dilution 1:30; Biolegend; Cat no 311406), PerCP/Cyanine5.5 anti-human CD3 (clone SK7 ...
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bioRxiv - Neuroscience 2024Quote: ... the iAstrocytes were stained with 1:50 PE anti-human HLA-A,B,C Antibody (BioLegend, 311406) and DAPI for 30 minutes at 4°C ...
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bioRxiv - Genomics 2024Quote: ... for 10 minutes at 4 °C and then stained with FITC - anti-human EPCAM (BioLegend CAT #32404) and PE - anti-CD45 (BioLegend ...
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bioRxiv - Immunology 2021Quote: ... for 1 hour and subsequently stained overnight at 4°C with mouse anti-human TCR Vα7.2-PE (Clone 3C10, Biolegend), mouse anti-human CD161-AF488 (Clone NKR-P1A ...
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bioRxiv - Immunology 2023Quote: ... P008_108)52 for 1h at 4°C followed by PE-rat anti-human IgG Fc (1:200, Biolegend) for 45 min at 4°C ...
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bioRxiv - Biochemistry 2022Quote: ... Two microliters of mouse anti-hexa His antibody (BioLegend) were added to the 10 mL sample and incubated for 1 h at room temperature ...
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bioRxiv - Microbiology 2021Quote: ... 100μl of HRP anti-His Tag Antibody (BioLegend, USA) (diluted 1:20000 ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... and a HRP anti-His tag antibody (Biolegend, #652504) diluted 1:1000 in Superblock (Thermo #37515 ...
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bioRxiv - Bioengineering 2020Quote: ... HRP conjugated anti-His tag secondary antibody (BioLegend, 652503) diluted 1:2000 in blocking buffer was then added to the plates and incubated at RT for 1 hour ...
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bioRxiv - Bioengineering 2024Quote: ... we used HRP anti-His tag antibody (BioLegend 652503).
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bioRxiv - Immunology 2022Quote: Mouse anti-His Tag antibody (clone J099B12, BioLegend #652502), goat anti-mouse IgG ...
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bioRxiv - Cancer Biology 2021Quote: ... then resuspended in FACS buffer containing 2 ug/ml APC/Cy7 anti-human HLA-A,B,C (BioLegend, #311425). Cells were incubated with the antibody on ice for 20 minutes in the dark ...
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bioRxiv - Immunology 2020Quote: ... Bacteria were opsonised in heat-inactivated human serum for 30 minutes at 37°C and labelled via incubation with 10μM carboxyfluorescein succinimidyl ester (CFSE) (Biolegend) for 30 minutes at 37°C ...
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bioRxiv - Immunology 2023Quote: ... Supernatant was discarded and cells were stained for 10 min at 4 °C with 2.5 μl Human TruStain FcX block (BioLegend) and 22.5 μl FACS buffer ...
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bioRxiv - Immunology 2024Quote: Up to 20,000 cells per sample (Table S1) were stained with the TotalSeq-C Human Universal Cocktail v1 (BioLegend) as per manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: Primary antibodies used in each sample were: mouse anti-HA 1:2000 (Biolegend, 901513; HEK293), rabbit anti Cav2.3 N-terminus 1:250 (Covalab ...
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bioRxiv - Molecular Biology 2020Quote: ... Purified anti-His tag mouse antibody was from BioLegend (USA). Secondary antibodies Alexa Fluor® 680 AffiniPure Donkey anti-mouse IgG and Alexa Fluor® 790 AffiniPure Donkey Anti-human IgG (H+L ...
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bioRxiv - Bioengineering 2023Quote: ... incubating the cells with APC anti-His Tag Antibody (BioLegend) for 20 minutes on ice ...
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bioRxiv - Biochemistry 2024Quote: ... Direct-Blot HRP anti-6×His Epitope Tag antibody (BioLegend) at 1:10,000 ...
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bioRxiv - Immunology 2023Quote: Human: Anti-human CD141 (clone M80, BioLegend), anti-human CD45RA (clone HI100 ...
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bioRxiv - Cell Biology 2020Quote: ... Primary antibodies targeting the various surface ligands and receptors as well as the corresponding control include: FITC anti-human HLA-A,B,C (Clone W6/32, Biolegend), FITC anti-human ICAM-1/CD54 (Clone DX2 ...
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bioRxiv - Systems Biology 2020Quote: ... The cells were incubated with each antibody for 45 min at −22–25°C after FcR blocking using Fc blocker (Human TruStain FcX, Biolegend) or anti-mouse CD16/CD32 antibody ...
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bioRxiv - Bioengineering 2020Quote: ... The following antibodies and dyes were used: anti-human HLA-A,B,C Pacific Blue (clone W6/32, BioLegend®), anti-HA Alexa Fluor 647 (clone HA.11 ...
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bioRxiv - Immunology 2021Quote: ... followed by a 30 minute incubation with the TotalSeq-C antibody pool and 5 μl of a PE/Cyanine7 anti-human CD8 antibody (BioLegend) at 4°C ...
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bioRxiv - Cancer Biology 2022Quote: ... and supernatants used for HLA-I IP in which purified anti-human HLA-A,B,C antibody (W6/32, BioLegend) was crosslinked to Protein G Dynabeads (Thermo Scientific ...
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bioRxiv - Systems Biology 2024Quote: ... Ultra-LEAF anti-human HLA-A,B,C antibody (clone W6/32, cat#: 311448) for immunopeptidomics was purchased from BioLegend. Polyethylenimine (PEI ...
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bioRxiv - Biochemistry 2024Quote: ... following an overnight incubation at 4°C with the primary antibody against residues 103-108 of human α-synuclein (BioLegend, 807801 [4B12] 1:1000 ...
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bioRxiv - Cancer Biology 2023Quote: ... and then incubated for one hour in PE anti-human CD71 Antibody in the dark at 4°C (1:100; Biolegend). Cells were washed twice with PBS and then and then analysed using a flow cytometer (FACsFortessa ...
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bioRxiv - Cancer Biology 2023Quote: ... and cell surface proteins were stained with the following antibodies (5 μl per test in 100 μl flow buffer) at 4 °C for 20 minutes: FITC anti-human CD45 (368508; BioLegend), FITC anti-human CD14 (325604 ...
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bioRxiv - Cancer Biology 2024Quote: ... and incubated for 30 min at 4°C with 1 µL of PE anti-human FasL antibody (clone NOK-1) from BioLegend. Nonspecific background signals were measured by incubating cells with the appropriate isotype-matched antibody ...
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bioRxiv - Immunology 2024Quote: ... cells were incubated for 25 minutes at 4°C with the following antibodies: BV711 anti-mouse/human CD11b (Cat# 101242; BioLegend), PE anti-mouse CX3CR1 (Cat# FAB5825P ...
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bioRxiv - Synthetic Biology 2024Quote: ... anti- human CD4 and anti-human CD8 (Biolegend), then assayed using a BD LSRFortessa flow cytometer (BD Biosciences).
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bioRxiv - Immunology 2021Quote: Leukocytes from whole blood and ETA samples were incubated with oligo-conjugated Ig-A/D/G/M for 10 min at 4°C followed by the addition of Human TruStain FcX™ (BioLegend) and 10 min incubation at RT ...
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bioRxiv - Cell Biology 2023Quote: ... cell suspensions were incubated for 20 min on ice with TotalSeq™-C anti-human Hashtag oligos (HTOs) (1:500, Biolegend, 394661 ...
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bioRxiv - Immunology 2024Quote: ... These cells were stained with a cocktail of mAb conjugated with DNA-barcoded oligonucleotide that are specific for immune cell lineages (TotalSeq™-C Human TBNK Cocktail, BioLegend) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2019Quote: HEK293 cells stably expressing EPO-eGFP and A1At-mCherry were incubated with 1ug/mL Brefeldin A (Biolegend) for 12 hr ...
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bioRxiv - Immunology 2022Quote: ... cells were incubated overnight at 4°C with purified monoclonal mouse anti-human ASC antibodies (TMS-1) (clone HASC-71) (Biolegend, California, USA) at a final concentration of 10 µg/ml diluted in 10% NGS + 0.5% Triton X-100 in PBS ...
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bioRxiv - Immunology 2021Quote: ... P815 cells were used as target cells for human CTLs and pulsed for 1h at 37°C with 1µg/ml anti-CD3 antibody (clone UCHT1, Biolegend, Cat no. 300438). They were washed and resuspended at 1x105 cells/ml in killing assay media (RPMI without phenol red + 2% FCS ...
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bioRxiv - Immunology 2020Quote: ... Then these PBMCs was incubated with mixed antibodies cocktail at 4 °C for 30 min (the antibodies cocktail including FITC-anti-human CD19 antibody (Biolegend, clone: SJ25C1), BV421-anti-human IgD antibody (Biolegend ...
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bioRxiv - Immunology 2021Quote: ... Slides were incubated ON at 4°C with mouse anti-human CD66b antibody (BioLegend, 305102, 1:100 for both IHC and IF), anti-human CD8 (Invitrogen ...
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bioRxiv - Immunology 2023Quote: Freshly sorted CD8+ T lymphocytes of four healthy volunteers were labelled with anti-human Hashtag antibodies TotalSeq-C (Biolegend, San Diego, CA) and were pooled together ...
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bioRxiv - Immunology 2023Quote: ... and then stained for 30 minutes at 4 °C using antigen probe (1.5 μL/well) and the following conjugated antibodies: APC anti-human CD3 (300412, BioLegend; 1 μL/well), PerCP/Cyanine 5.5 anti-human CD19 (302230 ...
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bioRxiv - Immunology 2023Quote: ... Human Fc receptors were blocked using Human TruStain FcX (Biolegend).
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bioRxiv - Immunology 2023Quote: ... Human Fc receptors were blocked using Human TruStain FcX (Biolegend). Phenotyping was performed on a NovoCyte Advanteon flow cytometer and FACS was performed on a BD LSRII.