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Citations for BioLegend :
51 - 100 of 5195 citations for Spleen Focus Forming Virus Proviral Integration Oncogene SPI1 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: CD4+ T cells were harvested from the lymph nodes and spleens of naive NR1 mice and enriched with a mouse naïve CD4 negative isolation kit (BioLegend) following the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2020Quote: ... T cells were isolated from spleen using MojoSort™ Mouse CD3 T Cell Isolation Kit (Biolegend Cat no; 480024) as per the manufacturers protocol ...
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bioRxiv - Immunology 2022Quote: ... CD8+ OT-I splenic T cells were isolated from crushed spleens of naïve OT-I mice using the Mouse CD8 T cell Isolation Kit (BioLegend). Sorted tumor APC populations were pulsed with 0.01 ng/mL SIINFEKL peptide (Invivogen ...
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bioRxiv - Immunology 2022Quote: ... Naïve CD4+ T cells were isolated from the spleen by using the MojoSort Mouse Naïve CD4+ T Cell Isolation Kit (#480040, BioLegend). CD4+ T cells isolated from the OT-II spleen were cocultured with BMDCs ...
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bioRxiv - Immunology 2023Quote: ... CD8+ T cells were isolated from the spleen using Mojosort Magnetic Separation System CD8 Naïve T cell Isolation Kit (BioLegend). Anti-CD3ε Ab (clone 145-2111C ...
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bioRxiv - Immunology 2023Quote: ... congenic CD45.1 mouse spleen was isolated and red blood cells were lysed with RBC lysis buffer (BioLegend, CA, USA). Leukocytes were then resuspended in complete RPMI with 1µg/mL high molecular weight Poly(I:C) ...
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bioRxiv - Immunology 2023Quote: ... CD4 T cells were purified from spleens and lymph nodes using MojoSort™ CD4 isolation kit and magnet (Biolegend). Purities of cells were determined for each experiment using flow cytometry ...
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bioRxiv - Immunology 2023Quote: ... Spleen standard cell suspensions were stained in the same conditions but used anti-CD45-FITC (Biolegend 109806, clone 104) to distinguish it from the other samples once pooled ...
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bioRxiv - Immunology 2024Quote: ... Naïve CD4+ T cells isolated from the mouse spleen using the MojoSort Mouse Naïve CD4 T Cell Isolation Kit (#480040, BioLegend) were stimulated in a plate coated with LEAF purified anti-mouse CD3ε antibody (clone 145-2111C ...
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bioRxiv - Immunology 2024Quote: ... Stained spleen or LN samples were fixed with 1-4% phosphate-buffered paraformaldehyde solution (FluoroFix or Fixation Buffer, Biolegend). To detect transcription factors and certain intracellular proteins ...
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bioRxiv - Immunology 2020Quote: ... Intracellular staining for Foxp3 was performed on single cell suspensions from spleen and LNs specifically using a FOXP3 Fix/Perm Kit (BioLegend).
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bioRxiv - Immunology 2020Quote: Spleens were harvested and dissociated using a cell mesh following which blood cells were removed using RBC Lysis Buffer (BioLegend). 2.5×106 splenocytes were stimulated with S or N peptide libraries (GenScript ...
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bioRxiv - Immunology 2021Quote: Mouse cytokines and chemokines were quantitated in graft and spleen tissue using the LEGENDplex Mouse Inflammation and custom plex panel (Biolegend) according to manufacturer’s instruction 22 ...
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bioRxiv - Immunology 2022Quote: Naïve CD4+ T cells were enriched from mouse spleen and lymph nodes by negative selection using MojoSort Mouse CD4 Naive T Cell Isolation Kit (MojoSort, Biolegend) following the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2022Quote: Viable mouse splenocytes were isolated by homogenizing spleen tissues through a 70 µm mesh followed by erythrocyte lysis using the RBC Lysis Buffer (Biolegend) according to the manufacturers’ instructions ...
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bioRxiv - Cell Biology 2022Quote: One million fixed BM and spleen cells were immunolabelled for one hour at room temperature with Ter119-PE (1:200, Biolegend) and 1ug/ml Hoechst 33342 (Thermofisher ...
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bioRxiv - Neuroscience 2022Quote: ... CD4+ T cells were isolated from the spleens and draining lymph nodes of 2D2 mice using the CD4+ isolation kit (BioLegend) and co-cultured with irradiated wild-type splenocytes at a ratio of 1:5 ...
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bioRxiv - Immunology 2021Quote: ... CD8+ T cells were then isolated from the spleens using the MojoSort™ Mouse CD8 T Cell Isolation Kit (BioLegend), following the manufacturer’s guidelines ...
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bioRxiv - Immunology 2023Quote: CD4+ and CD8+ T cells were isolated from the spleen lymphocytes of healthy mice using MojoSort mouse CD4 nanobeads and a MojoSort mouse CD8α selection kit (BioLegend), according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2023Quote: ... were isolated from single cell suspension of lymph nodes and spleen by negative selection using the MojoSort Mouse CD8 T cell isolation kit (BioLegend). Alternatively ...
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Mice generated with induced pluripotent stem cells derived from mucosal-associated invariant T cellsbioRxiv - Immunology 2023Quote: ... CD3+ T cells were purified from C57BL/6 spleen cells with MojoSort mouse CD3 T cell isolation kit (Biolegend, USA), while CD8+T cells were isolated by adding biotin-labeled anti-CD4 antibody into MojoSort mouse CD3 T cell isolation kit ...
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bioRxiv - Immunology 2023Quote: ... CD45.1+ GFP+ OT-II CD4+ T cells were purified from mouse spleens using magnetic beads (CD4 T cell MojoSort isolation kit, Biolegend) and activated with 10μg/ml recombinant mouse IL-2 IS (Miltenyi ...
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bioRxiv - Immunology 2024Quote: ... CD4+ T-cells were enriched from the spleen of OT-II mice using the MojoSort mouse CD4+ T-cell isolation kit (BioLegend) and labelled with CellTrace Violet (5 μM ...
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bioRxiv - Immunology 2023Quote: ... CD8+ T cells were enriched from the spleen of OT- I mice using the MojosortTM Mouse CD8+ T cell isolation kit (Biolegend) and stained with 5µM CellTrace Violet (Thermo Fisher ...
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bioRxiv - Microbiology 2021Quote: ... or Lassa virus (LASV) GPC peptide pool together with 5μg/ml Brefeldin A (Biolegend). Following surface staining with Live/Dead-APC/Cy7 ...
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bioRxiv - Microbiology 2021Quote: ... or Lassa virus (LASV) GP peptide pool together with 5μg/ml Brefeldin A (Biolegend). Following surface staining with Live/Dead-APCCy7 ...
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bioRxiv - Immunology 2021Quote: Naïve CD8+ T cells (OT-1 or Pmel-1) were isolated from pooled spleen and lymph nodes using MojoSort™ mouse CD8 T cell isolation kit (Biolegend) following manufacturer’s instruction ...
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bioRxiv - Immunology 2023Quote: ... CD3+ T cells were isolated from spleens of 6-to-8-week-old OT-II mice by MojoSort mouse CD3+ T cell isolation kit (Biolegend 480024). Stimulated FLS were washed with PBS and co-cultured with CD3+ T cells at a concentration of 5-10×105 cells/mL in 10% RPMI for 5 days with either the cognate OVA323-339 antigen (2μg/mL ...
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bioRxiv - Cancer Biology 2020Quote: ... Other cytokines were assayed using the LEGENDPlex multiplex assay (Human Anti-Virus Response Panel, BioLegend). Fluorescence was analyzed using a FACS Canto II (BD Biosciences).
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bioRxiv - Immunology 2022Quote: ... Samples were thawed and used undiluted in LEGENDplex™ Human Anti-Virus Response Panel (Biolegend) according to manufacturer’s instructions to measure different cytokines ...
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bioRxiv - Microbiology 2022Quote: ... a LegendPlex Human Anti-Virus Response Panel were used according to the manufacturer’s directions (BioLegend). Briefly ...
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bioRxiv - Immunology 2021Quote: ... five groups of cells were pre-labelled with mouse HashTag oligos (HTO) on cell surface: spleen T cell control group (SPT ctrl) with hashtag 1 (BioLegend, Cat# 155801); spleen T cell IL-1β/IL-23 stimulation group (SPT stim ...
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bioRxiv - Immunology 2020Quote: ... CD4T cells were negatively isolated from the digested spleen and LNs of indicated donor mice with Mouse CD4T Cell Isolation Kit (STEMCELL Technologies 19852 or BioLegend 480033) according to the manufacturer’s protocol and labeled with 1.0 μM CFSE (Thermo Fisher ...
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bioRxiv - Cancer Biology 2023Quote: ... CD8+ T cells were isolated from the spleens of C57BL/6 mice using the MojoSort™ Mouse CD8+ T Cell Isolation Kit (Biolegend, #480035), followed by 1X Red Blood Cell lysis buffer (eBioscience ...
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bioRxiv - Microbiology 2023Quote: Cytokine levels in the serum and spleen of mice infected with SPN were measured using ELISA MAX™ Standard Set Mouse IL-6 (BioLegend 431301) and ELISA MAX™ Standard Set Mouse IL-1β (BioLegend 432601 ...
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bioRxiv - Microbiology 2022Quote: ... single cell suspensions from mouse spleens were stained with fluorescein isothiocyanate (FITC)-conjugated anti-Ly-6A/E (Sca-1) mAb (Biolegend; San Diego, CA). To analyze erythrocyte maturation ...
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bioRxiv - Immunology 2020Quote: ... DCs were isolated from spleens and lymph nodes of C57BL/6 mice using a MojoSort™ Mouse Pan Dendritic Cell Isolation Kit (BioLegend cat. 480097). DCs were cultured with supernatants of necrotic keratinocytes generated by an overnight culture of UV-irradiated (2 J/m2/s for 40 min ...
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bioRxiv - Microbiology 2023Quote: 2 × 106 single cell suspensions prepared from the lung or spleen were resuspended in 200 ul of PBS with 2% fetal bovine serum and blocked with TruStain fcX (BioLegend, San Diego, CA). T cell subsets were identified with antibodies against CD45 (clone 30-F11 ...
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bioRxiv - Immunology 2023Quote: 1x106 cells/mL of Naïve CD4+ T cells were isolated from murine spleen and lymph-nodes via magnetic cell sorting (MojoSort, BioLegend, San Diego, CA, USA). Post isolation ...
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bioRxiv - Immunology 2023Quote: CD3+ T cells were purified from BALB/c mouse spleen cells with the MojoSort mouse CD3 T cell isolation kit (Biolegend, San Diego, CA) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... The upper phase of plasma was saved and subjected to LEGENDplex Anti-Virus Response Panel (740446) (BioLegend) or LEGENDplex Mouse Inflammation Panel (740446 ...
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bioRxiv - Cancer Biology 2023Quote: Flow cytometry viability staining was conducted by suspending murine spleen and tumor single cell suspensions in Zombie Violet fixable viability kit (Cat # 423114, BioLegend, San Diego, CA, USA) according to manufacturer instructions for 30 minutes at room temperature ...
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bioRxiv - Immunology 2023Quote: ... Cytokines were quantified by a fluorescence-encoded bead-based multiplex assay (LEGENDplex Mouse Anti-Virus Response Panel, BioLegend), per manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... Samples were analyzed using the LEGENDplex™ Human Anti-Virus Response Panel multiplex assay (BioLegend, San Diego, CA, USA) following the manufacturer’s protocol ...
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bioRxiv - Immunology 2020Quote: ... Negative control ‘decoy’ tetramers were similarly assembled using biotinylated Nipah virus soluble glycoprotein 65 and streptavidin-PerCP Cy5.5 (BioLegend). PBMC were washed in cold PBS and seeded at 1 × 106 cells/well in 96-well round bottom plates and with combinations of RBD and decoy tetramers by incubation for 30 min on ice ...
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bioRxiv - Immunology 2020Quote: ... Negative control ‘decoy’ tetramers were similarly assembled using biotinylated Nipah virus soluble glycoprotein 65 and streptavidin-PerCP Cy5.5 (BioLegend).
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bioRxiv - Immunology 2021Quote: Paired maternal and infant plasma were thawed and tested in the 13-plex LegendPlex Human Anti-Virus Response Panel kit (Biolegend), to quantify levels of IL-1β ...
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bioRxiv - Immunology 2022Quote: ... a multiplex cytokine quantification (LEGENDplex HU Anti-Virus Response Panel (13-plex) and LEGENDplex HU Th Cytokine Panel (13-plex) (cat# 740390 and cat# 740722, BioLegend) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... and was analyzed with the DropArray (Curiox Biosystems) and the MAGPIX System (Luminex) or with LEGENDPlex Human Anti-Virus Response Panel (13-plex, BioLegend) and was analyzed with LSRII (BD ...
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bioRxiv - Biochemistry 2019Quote: ... After transfection cell supernatants were collected and subjected to cytokine production analysis using LEGENDplex™ Mouse Anti-Virus Response Panel (13-plex) (BioLegend) on BD FACS Canto II flow cytometer using BD FACSDiva 8.0 software for acquisition and BioLegend’s LEGENDplex™ Data Analysis Software for data analysis.