Labshake search
Citations for BioLegend :
51 - 100 of 6001 citations for SpectraDye Antibody Labeling Kit 550 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Genomics 2021Quote: ... for RNA library construction while modifications for CITE-seq ADT and cell hashing HTO library preparation were performed according to the TotalSeq™-A Antibodies and Cell Hashing with 10x Single Cell 3’ Reagent Kit v3 or v3.1 single index protocol (BioLegend). The modifications include the addition of ADT and HTO additive primers to a final concentration of 0.2 uM in the cDNA amplification reaction (step 2.2) ...
-
bioRxiv - Cell Biology 2021Quote: ... Unfractionated or c-Kit-enriched BM cells were then incubated with purified rat anti-mouse lineage antibodies (CD3, BioLegend, 100202 ...
-
bioRxiv - Immunology 2024Quote: ... coupled with the protocol TotalSeq™-A Antibodies and Cell Hashing with 10x Single Cell 3’ Reagent Kit v3 or v3.1 (Single Index) from BioLegend.
-
bioRxiv - Neuroscience 2022Quote: ... Antibodies (BioLegend) used were as follows:
-
bioRxiv - Molecular Biology 2020Quote: ... and c-Kit on mast cells were analyzed by Flow cytometry using fluorochrome labelled antibodies (PE-CY7–conjugated anti-FcεRIα, MAR-1 Biolegend #134317; allophycocyanin-CY7–conjugated anti–c-Kit, 2B8, Biolegend, #105825). The YFP+ cells were FACS-sorted and deletion of the Gata2 gene was determined by PCR to be greater than 99%.
-
bioRxiv - Cancer Biology 2020Quote: ... All other antibodies used for immune fluorescence and flow cytometric analysis and human IL-1beta ELISA kit were acquired from Biolegend. Human was obtained from In vitro experiments were conducted using complete culture media consisting of RPMI 1640 medium supplemented with 20 mM HEPES ...
-
bioRxiv - Physiology 2022Quote: ... Samples were incubated with anti-c-kit rat monoclonal antibody (1:500 v/v, ACK2, BioLegend, San Diego, CA, USA) overnight (4ºC) ...
-
bioRxiv - Genomics 2020Quote: Cell and nuclei labelling were performed according to an adapted Biolegend cell hashing protocol (TotalSeq™-A Antibodies and Cell Hashing with 10x Single Cell 3’ Reagent Kit v3 3.1 Protocol, Biolegend). Briefly ...
-
bioRxiv - Cell Biology 2022Quote: ... or P14 bone marrow cells from 2-4 mice per genotype were c-kit selected and stained with tagged Total-Seq B antibodies (Biolegend) to CD150 ...
-
bioRxiv - Cancer Biology 2023Quote: ... Comprehensive surface immunophenotyping was performed using LEGENDScreen Human PE Kit containing 371 pre-titrated antibodies and 354 unique specificities (BioLegend). The experimental run was multiplexed using GFP+ NCI-H358 cells resistant to sotorasib combined with non-fluorescent ...
-
bioRxiv - Cancer Biology 2023Quote: ... were performed as we described previously48 and according to the Mission Bio protocol using the custom-designed 37-gene myeloid panel kit and 48 oligo-conjugated antibodies against all major BM cell types (Biolegend). Briefly ...
-
bioRxiv - Cell Biology 2022Quote: ... Antibodies used were pericentrin (PCNT) antibody (PRB-432C-Biolegend, Covance Cat# PRB-432C ...
-
bioRxiv - Genomics 2020Quote: ... oligonucleotide-conjugated antibodies and fluorescently labeled antibodies (Biolegend, USA) were both added at a 1:100 dilution in the same buffer as in the initial permeabilization step and incubated for 1h ...
-
bioRxiv - Plant Biology 2023Quote: ... Then 4 µg of primary antibodies (αHA antibody: Biolegend, #901502 ...
-
bioRxiv - Microbiology 2022Quote: Lung tissue leukocyte samples were subjected to viability staining and blocking of Fc-receptors using LIVE/DEAD™ Fixable Blue Dead cell stain kit (life technologies) and an anti-CD16/32 antibody (clone 93, BioLegend). Cells were then washed and incubated with a staining mix containing antibodies against the following murine antigens ...
-
bioRxiv - Immunology 2022Quote: ... Primary antibodies, anti-PDGFRa (AB Online, # ABIN726620), anti-Kitl (R&D, #AB- 455-NA) and anti-c-Kit (Biolegend, clone 2B8) were then stained at a 1:200 dilution for 72hrs ...
-
bioRxiv - Cancer Biology 2022Quote: Oligo-labeled antibody staining was done on both melanoblasts and melanom cells using the TotalSeq™-A Antibodies and Cell Hashing with 10× Single Cell 3′ Reagent Kit (Biolegend). The HashTag Oligonucleotidess used for the analysis are mHTO01 ...
-
bioRxiv - Neuroscience 2023Quote: ... libraries were prepared according to the Chromium Single Cell 3ʹ with Feature Barcoding technology for Cell Surface Protein Reagent Kit v3 protocol (10X Genomics) with modifications for incorporation of TotalSeq™-A HTO antibodies (clones M1/42; 30-F11, BioLegend). Briefly ...
-
bioRxiv - Immunology 2024Quote: Cells were stained with 20 µL of surface antibody cocktail with Zombie NIR fixable viability kit (1:400-1000, Biolegend, 423105) in PBS for 20 minutes on ice ...
-
bioRxiv - Neuroscience 2023Quote: ... the following primary antibodies and secondary antibodies were used: mouse anti-HA antibody (1:100) (16B12, BioLegend), rabbit anti-HA antibody (1:200 ...
-
bioRxiv - Pharmacology and Toxicology 2023Quote: ... antibodies after blocking by anti-CD16/32 antibodies (BioLegend 101302). Dead cells were excluded via 7-aminoactinomycin D staining.
-
bioRxiv - Neuroscience 2023Quote: ... At the antibody labelling stage oligonucleotide tagged antibodies (Hashtags, Biolegend) were added with the other cell type specific antibodies ...
-
bioRxiv - Cell Biology 2024Quote: ... the following antibodies were used (all antibodies were from BioLegend): 1:200 FITC-conjugated anti-CD45 ...
-
bioRxiv - Cell Biology 2024Quote: ... the following antibodies were used (all antibodies were from BioLegend): 1:100 AF488-conjugated anti-F4/80 ...
-
bioRxiv - Microbiology 2020Quote: ... coli antibody (BioLegend). Densitometry analysis was performed on an AlphaImager HP system using the manufacturer’s supplied AlphaView software ...
-
bioRxiv - Immunology 2022Quote: ... Antibodies from Biolegend include CD16 BV570 (Clone 3G8 ...
-
bioRxiv - Immunology 2020Quote: ... Antibodies from Biolegend include ...
-
bioRxiv - Immunology 2022Quote: ... Hashing antibodies (Biolegend, TotalSeq™-C0251 anti-human Hashtag 1-10 Antibodies ...
-
bioRxiv - Immunology 2023Quote: ... Hashtag antibodies (Biolegend) were added to samples (Supplementary Table 11 ...
-
bioRxiv - Cell Biology 2024Quote: ... Antibodies from BioLegend: Galectin-3 (1:1000 for WB ...
-
bioRxiv - Immunology 2022Quote: ... The following reagents and anti-mouse antibodies were used for flow cytometry: Zombie Violet™ fixable viability kit (BioLegend, San Diego, CA), CD45 (clone 30-F11 ...
-
bioRxiv - Cell Biology 2023Quote: ... density centrifugation or 1X RBC Lysis Buffer (eBioscience) and stained with a combination of fluorochrome-conjugated antibodies: c-Kit (BD Biosciences, BioLegend clone 2B8), Sca-1 (BioLegend clone D7) ...
-
bioRxiv - Genetics 2023Quote: ... were isolated by 1X Red Blood Cell lysis Buffer (eBioscience) and stained with a combination of fluorochrome-conjugated antibodies: c-Kit (BD Biosciences, BioLegend clone 2B8), Sca-1 (BioLegend clone D7) ...
-
bioRxiv - Bioengineering 2024Quote: ... were first stained with Zombie Aqua™ fixable viability kit (1:500 dilution in PBS) and Fc-blocked with TruStain FcXTM (anti-mouse CD16/32) antibody (BioLegend, CA, U.S.A.) (1:20 dilution in PBS (1% BSA) ...
-
bioRxiv - Immunology 2021Quote: ... Preconjugated antibodies were obtained from Fluidigm or purified antibodies from Biolegend were conjugated in house using Maxpar Conjugation kits (Fluidigm) ...
-
bioRxiv - Neuroscience 2020Quote: Primary antibody incubation was performed using mouse 6E10 monoclonal antibody (Biolegend) against human Aβ diluted (1:200 ...
-
bioRxiv - Microbiology 2020Quote: ... 50 μg/mL anti-TLR4 antibody or isotype control antibody (Biolegend), or 100 ng/mL E ...
-
bioRxiv - Genomics 2021Quote: ... the antibody solution of 52 TotalSeqC antibodies (BioLegend; Supplementary Table S1) was washed on a pre-wet Amicon Ultra-0.5 Centrifugal Filter to remove sodium azide ...
-
bioRxiv - Cancer Biology 2022Quote: ... Secondary antibody used was anti-human IgG Fc antibody (Biolegend, 410720). All the respective isotype antibodies used were procured and used as per the vendor’s instructions.
-
bioRxiv - Cell Biology 2023Quote: ... TotalSeq™-A0304 anti-mouse Hashtag 4 Antibody) (BioLegend, TotalseqA antibodies). The 4 biological replicates of each time point were stained with one of the 4 unique hash antibodies ...
-
bioRxiv - Neuroscience 2023Quote: ... The primary antibodies used include anti-Aβ antibodies 6E10 (Biolegend 803002), anti-GFAP (Dako Z0334) ...
-
bioRxiv - Immunology 2023Quote: ... or isotype control antibodies (the above antibodies were all from Biolegend) at room temperature for 15 min.
-
bioRxiv - Cancer Biology 2020Quote: ... and 200 μl of primary antibody (1:200 anti-αSMA antibody, eBioscience, clone 1A4, efluor 570; 1:200 anti-CD31 antibody, Biolegend, clone MEC13.3 ...
-
bioRxiv - Immunology 2024Quote: ... and a secondary antibody goat anti-mouse antibody conjugated with APC or rat anti-mouse antibody conjugated with Alexa Fluro 700 (BioLegend). These stained cells were fixed with fixation buffer (BioLegend ...
-
bioRxiv - Cancer Biology 2021Quote: ... antibody and the isotype control antibody (PE Mouse IgG2b, Biolegend, catalog#:400314) has a maximum excitation at 575 nm ...
-
bioRxiv - Immunology 2021Quote: ... hashed antibody and 0.5 tests of anti-CD8 hashed antibody (Biolegend 344753) were also used.
-
bioRxiv - Physiology 2021Quote: ... antibody and APC anti-mouse CD326 (EpCAM) antibody (clone G8.8, 118214, Biolegend) were used in a 1:50 and 1:100 dilution ratio ...
-
bioRxiv - Microbiology 2022Quote: ... Following antibodies were used: PE/Cyanine7 anti-human CD40 antibody (5C3, BioLegend); PE/Cyanine7 Mouse IgG1 ...
-
bioRxiv - Microbiology 2022Quote: ... All antibodies and isotype matched control antibodies are commercially available (BioLegend, Invitrogen).
-
bioRxiv - Cancer Biology 2023Quote: ... Flow Cytometry primary antibodies and working dilutions: BV421-CD19 antibody (BioLegend, #115537); PE-CD276 (1:100 ...