Labshake search
Citations for BioLegend :
51 - 100 of 2804 citations for Cyclohexyl 2 3 4 5 trifluorophenyl ethyl ketone since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2020Quote: ... PBS supplemented with 0.1 µg/ml antifade-4 6-diamidino-2-phenylindole (DAPI) (BioLegend, USA) was added to the cells for at least 30 min before imaging ...
-
bioRxiv - Bioengineering 2024Quote: ... Blocking occurred using 5% BSA in TBST and antibody probing using 2% BSA in TBST at the indicated dilutions: α-pERK1/2 (Biolegend 369502) 5000x ...
-
bioRxiv - Cell Biology 2022Quote: ... cells were incubated with Alexa Fluor 488 anti-mouse/human Mac-2 (Galectin-3) antibody (125410, Biolegend) or alternatively with Brilliant Violet 421 anti-mouse/human Galectin-3 antibody (125416 ...
-
bioRxiv - Immunology 2024Quote: ... about 3-4 million CD8+ T cells were blocked with Fc receptor blocking solution (Human TruStain FcX, BioLegend, #422302) for 10 minutes on ice and stained with BEAM-T assemblies for 15 minutes on ice ...
-
bioRxiv - Immunology 2021Quote: ... 2×106 cells were incubated for 5 hours with Brefeldin A (BioLegend, San Diego, California) and Monensin (BioLegend ...
-
bioRxiv - Immunology 2022Quote: A 5-plex SARS-CoV-2 Variants Neutralizing Antibody Panel (BioLegend LEGENDplex, Cat. No. #741174) was used to measure the neutralization antibody concentration ...
-
bioRxiv - Immunology 2022Quote: ... Omicron RBD (BA.2 or BA.5) were labeled with TotalSeq-C0971 Streptavidin (Biolegend, 405271) and TotalSeq-C0972 Streptavidin (Biolegend ...
-
bioRxiv - Immunology 2024Quote: ... Cells were fixed for 5 to 10 min at room temperature in 4% paraformaldehyde fixation buffer (BioLegend). Cells were permeabilized for 30 min at 4°C in PhosFlow Perm Buffer III (BD Biosciences) ...
-
bioRxiv - Immunology 2023Quote: ... the cells were stained for 5 min at 4°C with biotinylated anti-CD19 antibody (1D3; BioLegend) to exclude CD19-positive B cells in the cell suspension using anti-biotin microbeads and LS columns ...
-
bioRxiv - Cell Biology 2023Quote: ... the samples were incubated with 4’,6-diamidino-2-phenylindole (DAPI) (422801, BioLegend, San Diego, CA) for 2 minutes at room temperature ...
-
bioRxiv - Immunology 2021Quote: ... and seeded in the antibody-coated flask at 3–5×105 cells/cm2 together with soluble 1 µg/mL anti-CD28 antibodies (clone 37.51; 102116, BioLegend). Cells were cultured for 48 hr at 37°C in a humidified 5% CO2 atmosphere ...
-
bioRxiv - Immunology 2024Quote: ... which is RPMI-1640-based and supplemented with 5 μg/mL recombinant mouse IL-3 (#575506 or #575508, BioLegend) as described in previous studies (6 ...
-
bioRxiv - Immunology 2020Quote: ... Tim-3 clone RMT 3-23 (BioLegend) and clone FAB1529 (R&D) ...
-
bioRxiv - Neuroscience 2024Quote: ... the cells were fixed with cold methanol for 3 min at room temperature and incubated overnight at 4 °C with an anti-HA antibody (diluted 1:2000) (Biolegend,). Cells were then rinsed three times with PBS and incubated for 1 h at room temperature with an Alexa647-conjugated goat anti-mouse antibody (diluted 1:1000 ...
-
bioRxiv - Cancer Biology 2024Quote: ... Residual red blood cells (RBC) were lysed incubating cell suspension for 3 minutes at 4°C with 1X RBC Lysis Buffer (BioLegend). Cell suspensions were washed with PBS ...
-
bioRxiv - Cell Biology 2022Quote: ... Both cell fractions were stained with cell hashing antibodies (1:1000, #2 for heart, #3 for blood, Biolegend) and Propidium iodide (1:100 ...
-
bioRxiv - Immunology 2024Quote: ... Cells were washed 3 times with PBS containing 2% FBS before 7-AAD viability staining solution (BioLegend, #420404) was added and cells were subjected to FACS (BD FACSAria 4-laser digital flow cytometer with FACSDiva v8 software) ...
-
bioRxiv - Cell Biology 2023Quote: ... Between 50,000 to 100,000 cells were resuspended in 10 uL of PBS supplemented with 2% FBS and 2 mM EDTA (hereafter named “FACS buffer”) together with 5 uL of TruStain FcX (BioLegend, San Diego, CA, USA). Cells were incubated with the FcX block for 10 minutes at RT ...
-
bioRxiv - Immunology 2021Quote: ... Naive P14 Thy1.1+ Il2ra−/− cells were isolated from 4-5 week old mice by depleting CD44hi cells (Biolegend biotin anti-mouse/human CD44 ...
-
bioRxiv - Cancer Biology 2024Quote: Pre-coat a 12-well plate overnight at 4 °C using CD3 antibody (5 μg/ml, 317326, Biolegend), and before inoculation ...
-
bioRxiv - Cancer Biology 2022Quote: ... TGFß (1 ng/ml; cat#763102) and IL-2 (5 ng/ml, cat# 575402; All Biolegend) were added along with T cell media (RPMI ...
-
bioRxiv - Immunology 2021Quote: ... and cells were instead stained with 4′,6-diamidino-2-phenylindole (DAPI; BioLegend, San Diego, CA, USA) and gated for live (DAPI- ...
-
bioRxiv - Cancer Biology 2020Quote: ... we incubated the nanowell array with 4 µg/ml PE anti-CD63 antibody (BioLegend, clone NVG-2) for 45 minutes at 37°C ...
-
bioRxiv - Immunology 2021Quote: ... CD8 BUV496 or BV650 (2:100 or 4:100; BD Biosciences Cat# 612942 or Biolegend Cat# 301042), and Live/Dead eFluor506 (5:1000 ...
-
bioRxiv - Bioengineering 2024Quote: ... they were incubated overnight at 4 °C with mouse anti-βIII tubulin (2 µg/mL, TUBB3, Biolegend). After the primary antibody incubation ...
-
bioRxiv - Systems Biology 2021Quote: ... deeply anesthetized mice (5% isoflurane) were retro-orbitally injected with 3 μg of anti-CD45 antibody (PerCP, clone 30-F11, Biolegend) 3 min prior to euthanasia [46,81] ...
-
bioRxiv - Neuroscience 2021Quote: ... The sections were washed again 3 × 5 minutes in PBS and incubated overnight with rat anti-phospho-TDP primary antibody (1:100, 829901, BioLegend), in a dilution buffer (0.05% Tween ...
-
bioRxiv - Immunology 2023Quote: ... Pooled cells from n = 3 - 5 mice/cohort were stained with PE- or APC-conjugated antibodies (from either BD or BioLegend) against murine F4/80 (clone ...
-
bioRxiv - Immunology 2023Quote: ... 106 of the CTV labeled T cells were stimulated in vitro for 3 or 5 days in cultures containing 100ng/ml IL-15 (BioLegend) or a combination of 100ng/ml IL-7 (BioLegend ...
-
bioRxiv - Immunology 2023Quote: BMMCs were generated from the BM cells of C57BL/6 mice (Japan SLC, Hamamatsu, Japan) by cultivation in the presence of 5 ng/mL of mouse IL-3 (BioLegend) as previously described 18 ...
-
bioRxiv - Cell Biology 2024Quote: ... the sperm smears were fixed for 10 min with 3.2 % paraformaldehyde and permeabilized with the Intracellular Staining Perm Wash Buffer for 3 x 5 min (421002, BioLegend, USA). Following permeabilization ...
-
bioRxiv - Microbiology 2021Quote: ... Sections were blocked with 10% sheep serum and 1% BSA in TBS for 30 min then incubated at 4°C overnight with 1 μg ml−1 rabbit polyclonal anti-tubulin β-3 IgG (Biolegend) and 1% BSA in TBS ...
-
bioRxiv - Cancer Biology 2022Quote: ... or TFH mimicking signals (including 0.02 μg/mL recombinant human IL-4, Peprotech; 0.05 μg/ml recombinant human IL-21, Peprotech, and 5 μg/mL anti-human CD40, Biolegend). Complete RPMI medium contained RPMI 1640 (Gibco ...
-
bioRxiv - Immunology 2020Quote: ... Th1 cells were differentiated for 4 days in the presence of plate-bound αCD3 (5 μg/ml; Biolegend, #100339), rIL-12 (25 ng/ml ...
-
bioRxiv - Microbiology 2021Quote: ... Primary CD4+ T cells were activated for 4-5 days on T25 flasks coated with 5μg anti-CD3 antibody (clone OKT3, Biolegend) in the presence of 2μg/ml soluble anti-CD28 antibody (clone CD28.2 ...
-
bioRxiv - Immunology 2020Quote: The following antibodies and reagents were used for flow cytometry: CD4 clone RM-4-5 (BD Biosciences, BioLegend, Tonbo), CD8α clone53-6.7 (BD ...
-
bioRxiv - Bioengineering 2024Quote: ... anti-mouse IgG2a/b-FITC (5 g/ml) and anti-mouse CD138-PE (4 ug/ml; BioLegend cat# 142504); ii ...
-
bioRxiv - Molecular Biology 2021Quote: ... or day 5 IL-2 cultured NK cells isolated using the MojoSort NK isolation kit from Biolegend, but instead of analysis by flow cytometry ...
-
bioRxiv - Immunology 2023Quote: ... rhIL-2 (50 Uml-1, Novartis) and anti-mIFN-γ (5 µg ml-1, clone XMG1.2, Biolegend) in the presence or absence of 30 mM NaCl ...
-
bioRxiv - Cancer Biology 2023Quote: ... supplemented with 5 % human AB serum (Gemini Bio) and 30 U / mL recombinant human IL-2 (BioLegend) [9] ...
-
bioRxiv - Cancer Biology 2023Quote: ... supplemented with 5 % human AB serum (Gemini Bio) and 30 U / mL recombinant human IL-2 (BioLegend). The viability of NALM6-GL cells was then assessed by adding 150 ug / mL D-Luciferin (PerkinElmer ...
-
bioRxiv - Cancer Biology 2022Quote: ... spun at 700 g for 3 minutes at 4°C and washed in MACS Buffer 3× before being incubated with the following antibodies: APC-conjugated rat anti-CD3 (5 μL/10×106 cells; Biolegend, 100408), PE-conjugated rat anti-CD8b (2.5 μL/10×106 cells ...
-
bioRxiv - Neuroscience 2022Quote: ... and 0.3% PBST for 3 days at 4°C on rotating incubator (NFH, 1:500, Abcam, ab4680; Tubulin β-III, 1:200, Biolegend, 801201). Whole muscles were washed in 0.3% PBST for 5 hours then incubated in secondary antibodies for 48h at 4°C on rotating incubator (AlexaFluor 568 ...
-
bioRxiv - Neuroscience 2020Quote: ... Permeabilization was followed with blocking step by incubating the samples for 2 hr in PBS containing 3% normal goat serum (NGS, 927503, BioLegend) and 3% BSA and then stained overnight at 4°C with the primary monoclonal antibody (mouse anti-radixin ...
-
bioRxiv - Immunology 2020Quote: ... Cocktail 2 was used to identify DE cells and contained 3 μl of anti-human CD5-APCeF780 antibody (clone L17F12, Biolegend), 2 μl of anti-human CD19-PECy7 antibody (clone HIB19 ...
-
bioRxiv - Immunology 2023Quote: ... Panels 2 and 3 incorporated anti-Digoxygenin (RD System, #611621), anti-FITC (Standard BioTools, #3174006B) and anti-Biotin (Biolegend, #409002) metal-tagged antibodies to detect the RNAscope staining output.
-
bioRxiv - Cancer Biology 2022Quote: ... protein expression (ADT) and hashtag (HTO) libraries were generated using Chromium Single Cell 3’ version 2 reagents (10x genomics and Biolegend) per manufacturers’ protocols ...
-
bioRxiv - Immunology 2022Quote: ... resuspended in 50 μl FACS-buffer containing the respective fluorochrome-coupled Abs (anti-TACI/CD267 PE, clone eBio8F10-3, eBioscience, anti CD138 PECy7, clone 281-2, Biolegend, anti CD19 BV510 ...
-
bioRxiv - Evolutionary Biology 2022Quote: ... Cells were centrifuged at 500× g at 4 °C and resuspended in 1/3 strength artificial seawater containing 2 μg/mL calcein AM (Enzo) and 100 nM sytox blue (BioLegend) to monitor viability ...
-
bioRxiv - Immunology 2024Quote: ... and APC anti-ligand (B7-H6: R&D, clone 875001; CD48: Biolegend, clone; BJ40; Nectin-2: Biolegend, clone TX31; LFA-3: Biolegend, cloneTS2/9 ...