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Citations for BioLegend :
51 - 100 of 1920 citations for 3 4 Fluorophenyl 5 fluorobenzoic acid since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... Th1 cells were differentiated for 4 days in the presence of plate-bound αCD3 (5 μg/ml; Biolegend, #100339), rIL-12 (25 ng/ml ...
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bioRxiv - Microbiology 2021Quote: ... Primary CD4+ T cells were activated for 4-5 days on T25 flasks coated with 5μg anti-CD3 antibody (clone OKT3, Biolegend) in the presence of 2μg/ml soluble anti-CD28 antibody (clone CD28.2 ...
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bioRxiv - Immunology 2020Quote: The following antibodies and reagents were used for flow cytometry: CD4 clone RM-4-5 (BD Biosciences, BioLegend, Tonbo), CD8α clone53-6.7 (BD ...
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bioRxiv - Bioengineering 2024Quote: ... anti-mouse IgG2a/b-FITC (5 g/ml) and anti-mouse CD138-PE (4 ug/ml; BioLegend cat# 142504); ii ...
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bioRxiv - Cancer Biology 2022Quote: ... spun at 700 g for 3 minutes at 4°C and washed in MACS Buffer 3× before being incubated with the following antibodies: APC-conjugated rat anti-CD3 (5 μL/10×106 cells; Biolegend, 100408), PE-conjugated rat anti-CD8b (2.5 μL/10×106 cells ...
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bioRxiv - Neuroscience 2022Quote: ... and 0.3% PBST for 3 days at 4°C on rotating incubator (NFH, 1:500, Abcam, ab4680; Tubulin β-III, 1:200, Biolegend, 801201). Whole muscles were washed in 0.3% PBST for 5 hours then incubated in secondary antibodies for 48h at 4°C on rotating incubator (AlexaFluor 568 ...
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bioRxiv - Immunology 2020Quote: 96-well flat-bottomed plates were pre-coated overnight at 4°C with 5 µg/ml anti-NKp30 (clone P30-15, Biolegend) and -CD18 (clone IB4 ...
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bioRxiv - Immunology 2021Quote: ... Splenocytes from uninfected or infected mice were incubated at 37C° in 5% CO2 for 4 h in medium containing Monensin and Brefeldin A solutions (Biolegend). Cells were then stained for surface antigens (TCRβ-PacificBlue and NK1.1-PE) ...
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bioRxiv - Immunology 2021Quote: Cell samples went through a series of centrifugations (400g, 5 minutes, at 4°C) and were stained with PE anti-CD45 (30-F11, BioLegend) in staining buffer (see flow cytometry section ...
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bioRxiv - Molecular Biology 2024Quote: ... IP was performed overnight at 4° C using 5 μg antibody (anti-H3K9me3 Diagenode, ref. C15410056; anti-HA.11 BioLegend ref ...
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bioRxiv - Immunology 2024Quote: ... isolated naïve splenic B cells were cultured at a density of 0,5 x 106 cells/ml in the presence of 25 μg/ml LPS and 5 ng/ml IL-4 and stained with anti-CD138 (BioLegend). B cell proliferation was assessed by CellTrace Violet (Thermofisher ...
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bioRxiv - Pathology 2023Quote: ... Ldlr−/− mice fed a chow diet or HC diet for 4 days were anesthetized using Isoflurane and injected intravenously with 5 μg anti-mouse TIM4-APC antibody (RMT4-54, BioLegend). They were then euthanized 5 minutes after injection by cervical dislocation and perfused through the portal vein with PBS and then with 4% ice-cold PFA ...
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bioRxiv - Immunology 2023Quote: Single-cell suspensions were washed with cell staining buffer twice (300g, 5 min, 4⁰C) and incubated in 100 μl of Cell Staining Buffer (10 ml; 420201, Biolegend) with TruStain FcX™ (1:100 ...
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bioRxiv - Cancer Biology 2023Quote: ... and bone marrow were washed with cell staining buffer twice (300g, 5 min, 4⁰C) and incubated in 100 μl of Cell Staining Buffer (10 ml; 420201, Biolegend) with TruStain FcX™ (1:100 ...
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bioRxiv - Immunology 2024Quote: ... cells were centrifuged at 400 g for 5 minutes at 4°C and resuspended in cell staining buffer (CSB; Biolegend). The number of cells in the suspension was established using the CountessTM II cell counter (Invitrogen).
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bioRxiv - Neuroscience 2024Quote: ... centrifuged at 1500 rpm for 5 min at 4°C and resuspended in 500µl Cell Staining buffer (BioLegend, Cat#: 420201) containing 1:4000 dilution of SYTOX Blue (ThermoFisher ...
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bioRxiv - Neuroscience 2024Quote: ... centrifuged at 1500 rpm for 5 min at 4°C and resuspended in 500µl Cell Staining buffer (BioLegend, Cat#: 420201) containing 1:4000 dilution of SYTOX Blue (ThermoFisher ...
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bioRxiv - Neuroscience 2024Quote: ... centrifuged at 1500 rpm for 5 min at 4°C and resuspended in 500µl Cell Staining buffer (BioLegend, Cat#: 420201) containing 1:4000 dilution of SYTOX Blue (ThermoFisher ...
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bioRxiv - Immunology 2021Quote: ... plates were coated at least 5 hours prior to use with 3 ug/ml plate-bound anti-CD3 antibody (clone OKT3; BioLegend, LEAF grade), 1 ug/ml soluble anti-CD28 antibody (BioLegend ...
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bioRxiv - Immunology 2024Quote: ... rmIL-3 (Biolegend) was added to media (1 ng/ml) ...
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bioRxiv - Microbiology 2020Quote: ... 5 × 105 cells/mL of CD14+ enriched cells were stimulated using recombinant human (rh) GM-CSF and rhIL-4 (BioLegend, USA) at a concentration of each 500 IU/mL in RPMI1640 media supplemented with 10% FCS at 37°C in 5% CO2 ...
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bioRxiv - Cancer Biology 2022Quote: ... then incubated with their respective primary antibodies diluted in 5% skim milk in PBST at 4 °C overnight: purified mouse anti-βIII-tubulin (1:1,000) (BioLegend, cat. 801202), monoclonal mouse anti-GAPDH (1:50,000 ...
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bioRxiv - Immunology 2023Quote: ... cells were washed three times (300g, 5 min, 4⁰C) in cell staining buffer and kept in FluoroFix™ Buffer (422101, Biolegend) prior to run in a BD FACSymphony™ A5 Cell Analyzer (BD Biosciences) ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were washed three times (300g, 5 min, 4⁰C) in cell staining buffer and kept in FluoroFix™ Buffer (422101, Biolegend) before run in a BD FACSymphony™ A5 Cell Analyzer (BD Biosciences) ...
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bioRxiv - Immunology 2022Quote: ... samples were pre-incubated for 5 minutes at 4°C with TruStain FcX™ (anti-mouse CD16/32) Fc block (clone 93; BioLegend). Samples were then stained for extracellular markers in the presence of Fc block for 30 minutes at 4°C protected from light using the following antibodies ...
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bioRxiv - Immunology 2024Quote: ... The fluorochrome-conjugated antibodies were as follows for flow cytometry: BV605 Rat Anti-Mouse CD4 (RM4-5/RM4-4; Biolegend #116027), PE-Cy™7 Rat Anti-Mouse CD8a (53-6.7 ...
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bioRxiv - Cancer Biology 2024Quote: ... or after a 4-day incubation period at 37°C with 5% CO2 (Day 4) following 30 min antibody staining and additional 15 min Annexin V APC binding (Biolegend, 640920). The antibodies used were as follow ...
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bioRxiv - Immunology 2020Quote: ... Unbound antibodies were removed by washing with PBS were added at 5 × 104 cells/well in the presence of 4 μg/mL brefeldin A (Biolegend, Cat # 420601), 5 μg/mL GolgiStop (BD Biosciences ...
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bioRxiv - Cell Biology 2024Quote: S6K phosphorylation was measured by intracellular staining in murine CD8+ T cells activated for 24 h with plate-bound anti-CD3 (coated at 5 µg / ml for 14 h at 4 °C in PBS; BioLegend, Cat. # 100331) and 1 µg / ml soluble anti-CD28 (BioLegend ...
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bioRxiv - Immunology 2023Quote: ... IL-4, IL-2, IL-6, IL-10, IL-17A, IL-17F, IL-5, IL-9, IL-13, IL-22; BioLegend Cat# 741044) and Mouse Custom696 Panel (CXCL1(KC) ...
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bioRxiv - Neuroscience 2022Quote: ... Cell suspensions were treated with anti-mouse-CD16/32 to block Fc receptors (BioLegend, 5 μg/mL, 20 min at 4 °C) before washing and transferring to PBS and treating with Zombie NIR (BioLegend ...
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bioRxiv - Immunology 2024Quote: ... PBMCs were stimulated as a single-cell suspension at a concentration of 4-5 x 106 cells/mL with the Cell Activation Cocktail (Biolegend, Cat # 423301) and incubated at 37°C with 5% CO2 ...
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bioRxiv - Bioengineering 2021Quote: ... or 25 ng/ml Interleukin-4 (IL-4) (Biolegend) were used to stimulate M1 or M2 polarization ...
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bioRxiv - Immunology 2021Quote: ... 5 ng/ml IL-5 (Biolegend; 581504), and 20 ng/ml IL-2 (Biolegend ...
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bioRxiv - Immunology 2023Quote: ... streptavidin cyanine 5 (5 μg/mL, BioLegend) for 1 h ...
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bioRxiv - Immunology 2020Quote: ... CD40 (3/23, BioLegend), CD115 (CSF-1R ...
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bioRxiv - Immunology 2022Quote: ... CD40 (3/23) (Biolegend), CD11b (M1/70) ...
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bioRxiv - Microbiology 2024Quote: ... caspase-3 (W20054B; Biolegend), cleaved caspase-3 (Asp175 ...
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bioRxiv - Immunology 2023Quote: ... TIM-3-BV711 (Biolegend), LAG-3-APC-eFluor 780 (Invitrogen) ...
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bioRxiv - Immunology 2023Quote: ... TIM-3-BV711 (Biolegend), LAG-3-APC-eFluor 780 (Invitrogen) ...
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bioRxiv - Cancer Biology 2022Quote: ... LAG-3 (BioLegend, 369212) or LFA-1 (BD ...
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bioRxiv - Cancer Biology 2023Quote: ... Tim-3 (BV510, BioLegend), LAG-3 (BV605) ...
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bioRxiv - Bioengineering 2023Quote: ... Histone 3 (BioLegend #819411), YAP/TAZ (Santa Cruz #sc-101199) ...
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bioRxiv - Cell Biology 2022Quote: ... half a million cells per sample were incubated for 10 min in 50 μl of PBS with 3% FSB and 5 μl of blocking buffer (BioLegend, San Diego, CA, USA) and then stained with 50x diluted TotalSeq-B cell feature barcode antibody (BioLegend ...
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bioRxiv - Immunology 2021Quote: ... Nonspecific binding was blocked by incubation for 10 min at 4 °C with anti-CD16/CD32 antibody (Biolegend, clone 93, 5 ng/ μL) antibody and stained with the appropriate antibodies for 15 min at 4 °C ...
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bioRxiv - Immunology 2022Quote: ... Cells were centrifuged for 5 min at 500 x g and 4 °C and stained with Fc receptor blocker (TruStain fcX™, BioLegend, 1:100) and viability stain (Zombie Aqua™ Fixable Viability Kit ...
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bioRxiv - Genomics 2021Quote: ... 5% normal goat serum in PBS for 1 hour and incubated at 4°C overnight with primary antibody (Biolegend, cat. 613402, dilution 1:500), then at room temperature for 3 hours with secondary antibody (Santa Cruz Biotechnology ...
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bioRxiv - Biochemistry 2020Quote: ... Recombinant galectin-3 (Biolegend # 599706) was then incubated either for 1-2 hrs at RT or overnight at 4°C ...
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bioRxiv - Immunology 2020Quote: ... 3) CD62L APC-Cy7 (BioLegend), huCD45 PE (BioLegend) ...
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bioRxiv - Immunology 2022Quote: ... LAG-3-BV711 (11C3C65, Biolegend) and TIGIT-BV605 (A15153G ...