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• Novel spirocyclic dimer, SpiD3, targets chronic lymphocytic leukemia survival pathways with potent preclinical effects

  Alexandria P Eiken, et al., bioRxiv - Cancer Biology 2024

  Quote: ... and then lysed using RBC Lysis Buffer (BioLegend) per manufacturer protocol before flow cytometry analysis ...
• Novel spirocyclic dimer, SpiD3, targets chronic lymphocytic leukemia survival pathways with potent preclinical effects

  Alexandria P Eiken, et al., bioRxiv - Cancer Biology 2024

  Quote: ... anti-CD5-FITC (BioLegend) and anti-CD19-PE (BD Biosciences ...
• Transgenic αβ TCR tonic signaling is leukemogenic while strong stimulation is leukemia-suppressive

  Telmo A. Catarino, et al., bioRxiv - Cancer Biology 2024

  Quote: ... plates were coated with 10 μg/ml anti-CD3 (145-2C11, Biolegend) for 2 h at 37 °C ...
• Transgenic αβ TCR tonic signaling is leukemogenic while strong stimulation is leukemia-suppressive

  Telmo A. Catarino, et al., bioRxiv - Cancer Biology 2024

  Quote: ... Sections were incubated overnight with rat anti-B220 (1:200, clone RA3-6B2, Biolegend), anti-CD3 (1:200 ...
• Transgenic αβ TCR tonic signaling is leukemogenic while strong stimulation is leukemia-suppressive

  Telmo A. Catarino, et al., bioRxiv - Cancer Biology 2024

  Quote: ... slides were incubated sequentially with avidin/biotin blocking system (Biolegend), goat anti-rabbit secondary antibody (Biovision) ...
• Increased interaction between connexin43 and microtubules is critical for glioblastoma stem-like cell maintenance and tumorigenicity

  James W. Smyth, et al., bioRxiv - Cancer Biology 2024

  Quote: ... antibodies against HA tag (Biolegend), or V5 tag (Cell Signaling Technology ...
• Hybrid cancer stem cells utilise vascular tracks for collective streaming invasion in a metastasis-on-a-chip device

  Alice Scemama, et al., bioRxiv - Cancer Biology 2024

  Quote: ... CD24 (APC, 1/200 dilution, Biolegend 311117) and E-cadherin (Alexa Fluor 647 ...
• HAF Prevents Hepatocyte Apoptosis and Hepatocellular Carcinoma through Transcriptional Regulation of the NF-κB pathway

  Karen Acuña Pilarte, et al., bioRxiv - Cancer Biology 2024

  Quote: ... Human recombinant TNF was purchased from BioLegend Inc (San Diego ...
• A high throughput CRISPR perturbation screen identifies epigenetic regulators impacting primordial germ cell development

  Liangdao Li, et al., bioRxiv - Developmental Biology 2024

  Quote: ... and Alexa Fluor® 647 mouse/human anti-SSEA1 antibodies (1:200, Biolegend; 125608) at 4°C for 1 hour ...
• Stereotyped cell lineage trees support robust development

  Xiaoyu Zhang, et al., bioRxiv - Developmental Biology 2024

  Quote: ... Cells were subsequently stained with CD47-PerCPCy5.5 and CD26-PE antibodies (Anti-human CD47 PerCP/Cy5.5 conjugate, Biolegend, Cat#323110 ...
• MAIT Cells Modulate Innate Immune Cells and Inhibit Colon Cancer Growth

  Olivia J. Cheng, et al., bioRxiv - Cancer Biology 2024

  Quote: ... anti-MR1 (Clone 26.5, Biolegend, San Diego, CA, USA) or an isotype control was used at 10 µg/ml.
• Novel spirocyclic dimer, SpiD3, targets chronic lymphocytic leukemia survival pathways with potent preclinical effects

  Alexandria P Eiken, et al., bioRxiv - Cancer Biology 2024

  Quote: ... Helix NP™ NIR (BioLegend) was added for live/dead discrimination prior to flow cytometry analysis.
• Novel spirocyclic dimer, SpiD3, targets chronic lymphocytic leukemia survival pathways with potent preclinical effects

  Alexandria P Eiken, et al., bioRxiv - Cancer Biology 2024

  Quote: ... Helix NIR (BioLegend) was used for live/dead discrimination prior to flow cytometry analysis ...
• MAIT Cells Modulate Innate Immune Cells and Inhibit Colon Cancer Growth

  Olivia J. Cheng, et al., bioRxiv - Cancer Biology 2024

  Quote: ... 50,000 Calcein-labeled (2 µM, Biolegend, San Diego, CA, USA) COLO 205 cells were co-cultured with 500,000 human whole leukocytes for 4 hours with or without 5-A-RU (50 nM ...
• Loss of NAT10 disrupts enhancer organization via p300 mislocalization and suppresses transcription of genes necessary for metastasis progression

  Ruhul Amin, et al., bioRxiv - Cancer Biology 2024

  Quote: ... CD127 (A7R34) from Biolegend; CD11c (N418) ...
• KDM6A Regulates Immune Response Genes in Multiple Myeloma

  Daphne Dupéré-Richer, et al., bioRxiv - Cancer Biology 2024

  Quote: ... All listed antibodies for flow cytometry are from Biolegend, unless otherwise specified and were used at 1:100 dilution for this experiment.
• An atlas of epithelial cell states and plasticity in lung adenocarcinoma

  Guangchun Han, et al., bioRxiv - Cancer Biology 2024

  Quote: ... Epcam-BV421 (118225, BioLegend), and Ecad-A488 (53-3249-80 ...
• Tamm-Horsfall protein augments neutrophil NETosis during urinary tract infection

  Vicki Mercado-Evans, et al., bioRxiv - Immunology 2024

  Quote: ... anti-MHC-II-APC-Fire750 (clone M5/114.15.2, catalog no. 107652; BioLegend) or anti-MHC-II-BV650 (clone M5/114.15.2 ...
• Tamm-Horsfall protein augments neutrophil NETosis during urinary tract infection

  Vicki Mercado-Evans, et al., bioRxiv - Immunology 2024

  Quote: ... and anti-CD45-BV510 (clone 30-F11, catalog no. 103138; BioLegend) or anti-CD45-BV605 (clone 30-F11 ...
• Tamm-Horsfall protein augments neutrophil NETosis during urinary tract infection

  Vicki Mercado-Evans, et al., bioRxiv - Immunology 2024

  Quote: ... anti-Ly6G-APC (clone 1A8, catalog no. 127614; BioLegend), Live/Dead Near IR (1:200 of stock ...
• Tamm-Horsfall protein augments neutrophil NETosis during urinary tract infection

  Vicki Mercado-Evans, et al., bioRxiv - Immunology 2024

  Quote: ... anti-Ly6G-APC (clone 1A8, catalog no. 127614; BioLegend), anti-MHC-II-APC-Fire750 (clone M5/114.15.2 ...
• Tamm-Horsfall protein augments neutrophil NETosis during urinary tract infection

  Vicki Mercado-Evans, et al., bioRxiv - Immunology 2024

  Quote: ... anti-CD11c-PerCP-Cy5.5 (clone N418, catalog no. 45-0114-82; eBioscience) or anti-CD11c-BV786 (clone N418, catalog no. 117335; BioLegend), anti-Ly6G-APC (clone 1A8 ...
• PP2A catalytic subunit alpha is critically required for CD8⁺ T cell homeostasis and anti-bacterial responses

  Xian Zhou, et al., bioRxiv - Immunology 2024

  Quote: ... with BV510-labeld anti-CD8α (clone:53-6.7; BioLegend), Super Bright 600-labeled anti-CD4 (Clone ...
• PP2A catalytic subunit alpha is critically required for CD8⁺ T cell homeostasis and anti-bacterial responses

  Xian Zhou, et al., bioRxiv - Immunology 2024

  Quote: ... APC-Cy7-labeled TCRβ (clone: H57-597; BioLegend), APC-labeled anti-CD127 (A7R34 ...
• Regulation of IFNγ production by ZFP36L2 in T cells is context-dependent

  Nordin D. Zandhuis, et al., bioRxiv - Immunology 2024

  Quote: ... in the presence of TGFB (Biolegend) and IL-2 (5ng/ml ...
• Regulation of IFNγ production by ZFP36L2 in T cells is context-dependent

  Nordin D. Zandhuis, et al., bioRxiv - Immunology 2024

  Quote: ... PD-1 blocking antibody (29F.1A12, Biolegend) was freshly added (10μg/ml ...
• Congenital T cell activation impairs transitional to follicular B cell maturation in humans

  Hugues Allard-Chamard, et al., bioRxiv - Immunology 2024

  Quote: ... CD24 (ML5, BioLegend), CD10 (HI10a ...
• Congenital T cell activation impairs transitional to follicular B cell maturation in humans

  Hugues Allard-Chamard, et al., bioRxiv - Immunology 2024

  Quote: ... Cells were fixed for 5 to 10 min at room temperature in 4% paraformaldehyde fixation buffer (BioLegend). Cells were permeabilized for 30 min at 4°C in PhosFlow Perm Buffer III (BD Biosciences) ...
• Congenital T cell activation impairs transitional to follicular B cell maturation in humans

  Hugues Allard-Chamard, et al., bioRxiv - Immunology 2024

  Quote: ... anti-GFP (FM264G; BioLegend; 1:200), CD127 (A019D5 ...
• Congenital T cell activation impairs transitional to follicular B cell maturation in humans

  Hugues Allard-Chamard, et al., bioRxiv - Immunology 2024

  Quote: ... CD127 (A019D5; BioLegend; 1:30), and FOXP3 (259D/C7 ...
• Congenital T cell activation impairs transitional to follicular B cell maturation in humans

  Hugues Allard-Chamard, et al., bioRxiv - Immunology 2024

  Quote: ... CXCR5 (J252D4; BioLegend; 1:30), CTLA4 (BNI3 ...
• Congenital T cell activation impairs transitional to follicular B cell maturation in humans

  Hugues Allard-Chamard, et al., bioRxiv - Immunology 2024

  Quote: ... CD10 (HI10a, BioLegend), CD45RB (MEM-55 ...
• Congenital T cell activation impairs transitional to follicular B cell maturation in humans

  Hugues Allard-Chamard, et al., bioRxiv - Immunology 2024

  Quote: ... recombinant human CD40L (TNFSF5; BioLegend) was added to the media ...
• Congenital T cell activation impairs transitional to follicular B cell maturation in humans

  Hugues Allard-Chamard, et al., bioRxiv - Immunology 2024

  Quote: ... Flow cytometry analysis of CTLA4-mediated trans-endocytosis was performed as previously described [6] using the True-NuclearTM Transcription Factor Buffer Set (BioLegend) and the following intracellular antibody panel ...
• A mutant ASXL1-EHMT complex contributes to heterochromatin dysfunction in clonal hematopoiesis and chronic monomyelocytic leukemia

  Zhen Dong, et al., bioRxiv - Cancer Biology 2024

  Quote: ... were used in combination with a Streptavidin-PerCP-Cy5.5 conjugate (Biolegend). Live/Dead eFluor 780 (Invitrogen ...
• A mutant ASXL1-EHMT complex contributes to heterochromatin dysfunction in clonal hematopoiesis and chronic monomyelocytic leukemia

  Zhen Dong, et al., bioRxiv - Cancer Biology 2024

  Quote: ... All antibodies were purchased from Biolegend and BD Bioscience ...
• A mutant ASXL1-EHMT complex contributes to heterochromatin dysfunction in clonal hematopoiesis and chronic monomyelocytic leukemia

  Zhen Dong, et al., bioRxiv - Cancer Biology 2024

  Quote: ... Ter-119 (Ter-119) (all from Mouse hematopoietic Lineage Biotin Panel, Biolegend) and IL-7Rα (A7R34 ...
• A mutant ASXL1-EHMT complex contributes to heterochromatin dysfunction in clonal hematopoiesis and chronic monomyelocytic leukemia

  Zhen Dong, et al., bioRxiv - Cancer Biology 2024

  Quote: ... and IL-7Rα (A7R34, Biolegend) were used in combination with a Streptavidin-PerCP-Cy5.5 conjugate (Biolegend) ...
• Tissue resident cells differentiate S. aureus from S. epidermidis via IL-1ß following barrier disruption in healthy human skin

  Julia C. Lang, et al., bioRxiv - Microbiology 2024

  Quote: ... or anti-hu HLA-DR clone LN3 (BioLegend, Cat# 327002) and the secondary antibodies Alexa Fluor™ 488 anti-rat IgG (Invitrogen ...
• Tissue resident cells differentiate S. aureus from S. epidermidis via IL-1ß following barrier disruption in healthy human skin

  Julia C. Lang, et al., bioRxiv - Microbiology 2024

  Quote: ... and IL-1β concentrations were determined with the ELISA MAX™ Deluxe Set Human IL-1β (BioLegend, Cat# 437004) in 96-well half area Costar assay plates (Corning ...
• Pediatric human nose organoids demonstrate greater susceptibility, epithelial responses, and cytotoxicity than adults during RSV infection

  Gina M. Aloisio, et al., bioRxiv - Microbiology 2024

  Quote: ... keratin 5 (1:2000, KRT5 for basal cells; BioLegend, Catalog number: 905503), SCGB1A1 (1:200 ...
• Hypoxia and TNF-alpha modulate extracellular vesicle release from human induced pluripotent stem cell-derived cardiomyocytes

  Margarida Viola, et al., bioRxiv - Cell Biology 2024

  Quote: ... and HSP70 (Biolegend, 648001, 1:500). Secondary antibodies were diluted 1:7500 in a mixture of 50% v/v of Intercept blocking buffer (LI-COR ...
• Tissue resident cells differentiate S. aureus from S. epidermidis via IL-1ß following barrier disruption in healthy human skin

  Julia C. Lang, et al., bioRxiv - Microbiology 2024

  Quote: ... anti-hu CD1a clone SK9 (BioLegend, Cat# 344902) or anti-hu HLA-DR clone LN3 (BioLegend ...
• Tissue resident cells differentiate S. aureus from S. epidermidis via IL-1ß following barrier disruption in healthy human skin

  Julia C. Lang, et al., bioRxiv - Microbiology 2024

  Quote: ... The panel of cytokines presented in Fig 5 was measured using the LEGENDplex™ Human Inflammation Panel 1 (13-plex) (BioLegend, Cat# 740809) kit ...
• Regionalized cell and gene signatures govern oesophageal epithelial homeostasis

  David Grommisch, et al., bioRxiv - Cell Biology 2024

  Quote: ... and CD104-AF647 (BioLegend, clone 346-11A, #123608) while simultaneously excluding CD45-FITC (BioLegend ...
• Regionalized cell and gene signatures govern oesophageal epithelial homeostasis

  David Grommisch, et al., bioRxiv - Cell Biology 2024

  Quote: ... Cells from the epithelial layer were sorted gating on single positive for CD326-PE/Cy7 (BioLegend, clone DECMA-1, #147310) and CD104-AF647 (BioLegend ...
• Regionalized cell and gene signatures govern oesophageal epithelial homeostasis

  David Grommisch, et al., bioRxiv - Cell Biology 2024

  Quote: ... while simultaneously excluding CD45-FITC (BioLegend, clone 30-F11, #103107) positive cells ...
• Regionalized cell and gene signatures govern oesophageal epithelial homeostasis

  David Grommisch, et al., bioRxiv - Cell Biology 2024

  Quote: Rat anti-CD3 (BioLegend, clone 17A2, #100201, 1:100), Rat anti-CD4 (BioLegend ...
• BAX and BAK expression triggers multiple IRE1 signaling outputs under ER stress

  Claudia Sepulveda, et al., bioRxiv - Cell Biology 2024

  Quote: ... anti-HA (1:2000; 901514, Biolegend) anti-IRE1alpha (1:1000 ...
• Single-Stranded DNA with Internal Base Modifications Mediates Highly Efficient Gene Insertion in Primary Cells

  Karen L. Kanke, et al., bioRxiv - Molecular Biology 2024

  Quote: ... Purified anti-TP63 (W15093A) (Biolegend, 687202). Mouse anti-ACT (Sigma-Aldrich ...