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Citations for BioLegend :
901 - 950 of 3262 citations for Recombinant Human SLAMF1 Protein His Avi tagged Biotinylated since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... Monoclonal mouse IgG1 anti-human CD64 FITC-conjugated (clone 10.1) and monoclonal mouse IgG2a anti-human CD59 PE-conjugated were from BioLegend, San Diego ...
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bioRxiv - Developmental Biology 2024Quote: ... Cells were subsequently stained with CD47-PerCPCy5.5 and CD26-PE antibodies (Anti-human CD47 PerCP/Cy5.5 conjugate, Biolegend, Cat#323110, 1:200; Anti-human CD26 PE conjugate, Biolegend, Cat#302705 ...
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bioRxiv - Immunology 2023Quote: Activation consisted of 10μg/mL plate-bound anti-human CD3 antibody (300437) and 1μg/mL soluble anti-human CD28 antibody (302902) purchased from BioLegend, USA ...
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bioRxiv - Immunology 2021Quote: ... and CD4+ CD25- and CD8+ T cells were purified using the MojoSort Mouse CD4 (supplemented with biotinylated anti-CD25) and CD8 T Cell Isolation Kits (BioLegend), respectively ...
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bioRxiv - Immunology 2022Quote: ... CD8+ T cells were enriched via negative selection using biotinylated antibodies at the concentration indicated in Table 1 and using MojoSort Streptavidin Nanobeads (BioLegend). Enrichments were completed in 1X HBSS (Ca2+ and Mg2+ free ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were incubated with 1.5% normal serum followed by incubation with the primary antibody (biotinylated anti-Aβ – 1:100, BioLegend, USA) for 1 hour ...
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bioRxiv - Cancer Biology 2022Quote: ... bone marrow was subjected to RBC lysis as above and incubated with a biotinylated anti CD133 antibody (Clone 315-2C11, Biolegend), followed by Streptavidin MicroBeads (Miltenyi) ...
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bioRxiv - Bioengineering 2022Quote: ... Streptavidin-coated nanovials were reconstituted at a five time dilution in Washing Buffer containing 140 nM (20 μg/mL) of each biotinylated antibody or cocktail of antibodies: anti-CD45 (Biolegend) and anti-IFN-γ (R&D Systems) ...
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bioRxiv - Neuroscience 2019Quote: ... Sections were incubated with 1.5% normal serum followed by incubation with the primary antibody (biotinylated anti-Aβ – 1:100, BioLegend, USA) for 1 hour ...
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bioRxiv - Immunology 2019Quote: ... Naive CD4+ and CD8+ T cells were isolated using a negative selection cocktail containing the following biotinylated mouse antibodies (Biolegend): TCR γ/δ (clone GL3) ...
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bioRxiv - Immunology 2019Quote: ... Pre-enrichment for antigen presenting cells prior to sorting was done with biotinylated anti-CD3 (clone 145-2C11) and anti-CD19 (clone 6D5) both from Biolegend. Cell suspensions were incubated with antibodies for 10 minutes at room temperature at a dilution of 1:200 ...
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bioRxiv - Immunology 2020Quote: ... biotinylated NP peptide loaded SLA monomers were freshly assembled into tetramer with streptavidin BV421 or BV650 (both from Biolegend, UK). Two million mononuclear cells were incubated with protease kinase inhibitor in PBS for 30 minutes at 37°C and tetramers added to the cells on ice for another 30 minutes ...
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bioRxiv - Immunology 2020Quote: ... RBD tetramers were assembled by combining biotinylated RBD with streptavidin-Brilliant Violet 421 or streptavidin-Brilliant Violet 650 (both BioLegend) at a molar ratio of 4:1 ...
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bioRxiv - Immunology 2020Quote: ... RBD tetramers were assembled by combining biotinylated RBD with streptavidin-Brilliant Violet 421 or streptavidin-Brilliant Violet 650 (both BioLegend) at a molar ratio of 4:1 ...
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bioRxiv - Synthetic Biology 2024Quote: ... Cells from the bone marrow were first stained with the following combination of biotinylated antibodies (lineage cocktail) for 30 minutes at 4 C: Gr-1 (RB6-8C5; Biolegend), Ter-119 (TER-119 ...
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bioRxiv - Immunology 2024Quote: ... for 40 min on ice or with biotinylated anti-MR1 antibody 26.5 (BioLegend, custom conjugation, used at 5 μg/ml) for 40 minutes on ice ...
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bioRxiv - Immunology 2023Quote: ... CD4 and CD8β cells were depleted from the T-IEL compartment using CD4 and CD8β biotinylated antibodies and magnetic streptavidin nanobeads (MojoSort) (Biolegend). CD8α+ cells were purified from CD4 and CD8β depleted T-IEL and lymph node T cells using the CD8α Positive Selection Kit (Stemcell Technologies) ...
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bioRxiv - Immunology 2023Quote: ... HLA-expressing cells were enriched with a positive magnetic sort using the biotinylated pan-HLA antibody (clone W6/32, Biolegend) in conjunction with the ThermoFisher CELLection Dynabead cell isolation kit ...
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bioRxiv - Cancer Biology 2023Quote: ... 90% of the thawed cells (>200 x 106) were stained with biotinylated antibodies against CD45 (1:50, clone HI30; Biolegend) and CD235a (1:50 ...
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bioRxiv - Immunology 2023Quote: ... A scaffold-only tetramer was also made with biotinylated S2hlx-Ex19-PShlx or FP-10-wt mixed with Streptavidin-BV421 (Biolegend). These probes did not contain the grafted epitope.
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bioRxiv - Immunology 2024Quote: ... immune cells extracted from mouse blood and tissues as described above were first stained with 40 µg/mL of biotinylated mDCIR1-ECD or mDCIR1-ΛιECD pre-conjugated with with ¼ molar equivalents of Streptavidin-Phycoerythrin (streptavidin-PE; Biolegend) in DPBS containing Ca2+ and Mg2+ (Gibco) ...
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bioRxiv - Immunology 2021Quote: ... Bound ligand was detected with a horseradish peroxidase (HRP)-conjugated anti-His antibody (652504, BioLegend, San Diego, CA, USA). After incubation for 1 hour at room temperature and washing ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... and a further 1 h incubation with anti-His HRP conjugate (BioLegend; San Diego, CA, USA; cat. no. 652504) diluted (1:2500 ...
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bioRxiv - Immunology 2020Quote: ... IL-2 immune complex (IL-2ic; mouse recombinant IL-2 + anti-IL-2 (JES6-1A12, BioLegend); molar ratio 2:1 ...
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bioRxiv - Immunology 2021Quote: ... in the presence of 1 μg/mL anti-CD28 and 1 ng/mL recombinant IL7 (BioLegend) for 48 hours ...
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bioRxiv - Immunology 2022Quote: ... Colon cells were either untreated or treated with 25 µg/ml recombinant mouse IFN-β1 (Biolegend.) Supernatant was collected for ELISA and cells were stained for flow.
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bioRxiv - Immunology 2023Quote: ... we cultured mouse T cells in mouse RP10 with recombinant mouse TGF-β1 (Biolegend Cat # 763104), rIL-12 ...
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bioRxiv - Immunology 2023Quote: ... DC2.4 cells were then treated with 5ng/ml Recombinant Mouse IFN-ψ (Biolegend, Cat. No:575302) for 24 hours ...
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bioRxiv - Cell Biology 2023Quote: ... Animals were then given retroorbital injections of 2.75×105 IU of recombinant mouse IFN-α (BioLegend), and the final 20 min of images with the same time intervals were collected ...
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bioRxiv - Immunology 2023Quote: ... in the presence of Th17 polarizing cocktails containing recombinant mouse IL-6 (10 ng/ml, Biolegend), mouse IL-1β (10 ng/ml ...
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bioRxiv - Immunology 2024Quote: The cytokine stimulation conditions were performed with commercially available recombinant IL-12 (100 ng/mL; BioLegend), recombinant IL-18 (100 ng/mL ...
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bioRxiv - Immunology 2024Quote: Bone marrow derived-DCs (BMDCs) were prepared using 10 ng/mL recombinant GM-CSF (Biolegend, #576306), as previously described [18] ...
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bioRxiv - Neuroscience 2020Quote: For analysis of LILRB2 expression on marmoset and human monocyte derived macrophages were first blocked with human TruStain FcX Fc blocking solution (Biolegend), then labelled with and without anti-human LILRB2 (5 µg/mL ...
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bioRxiv - Bioengineering 2021Quote: ... Naïve T cell marker staining was performed with APC/Cy7 mouse anti-human CD45RA (#304128) and BV510 mouse anti-human CD45RO (#304232) antibodies from Biolegend.
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bioRxiv - Immunology 2021Quote: ... Tonsillar IgD+ B cells were isolated from human tonsil cells by positive selection using biotin-anti-human IgD mAb (clone IA6-2; 348212, Biolegend) and MagniSort™ Streptavidin Positive Selection Beads (MSPB-6003-74 ...
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bioRxiv - Immunology 2022Quote: ... PBMCs were surface stained with series of antibodies (anti-human CD158 [KIR2DL1/S1/S3/S5]-FITC [BioLegend, Cat#339504], anti-human CD158b [KIR2DL2/L3, NKAT2]-FITC [BioLegend, Cat#312604] ...
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bioRxiv - Cancer Biology 2022Quote: ... Antibodies used for staining of human tumor cells included: anti-human EpCAM-FITC (BioLegend 324204, clone 9C4, dilution 1:30), anti-human/mouse CD49f-APC (BioLegend 313616 ...
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bioRxiv - Biophysics 2022Quote: ... Monoclonal antibodies against human Glycophorin AB (CD235ab) (HIR2; catalog number: 306602) and human CD47 (CC2C6; catalog number: 323102) were purchased from BioLegend.
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bioRxiv - Immunology 2019Quote: ... Supernatants were analyzed by using either the Legendplex Multi-Analyte Flow Assay Kit for human CD8/NK cells or the human Th cytokine panel (Biolegend).
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bioRxiv - Immunology 2021Quote: ... containing anti-CD9 (human HI9a, mouse MZ3), anti-CD63 (human H5C6, mouse NVG-2), anti-CD81 antibodies (human 5a6, mouse Eat-2) from Biolegend, and anti-human ACE2 (R&D Biosystems cat no ...
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bioRxiv - Microbiology 2021Quote: ... Mouse IgG and human IgG were detected with separate secondary antibodies (goat anti-human IgG Fc, goat anti-mouse IgG Fc, BioLegend) at a dilution of 1:4,000 in blocking buffer and PNPP as described above.
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bioRxiv - Immunology 2020Quote: ... For human cytokine IL-4 in the supernatant of human ILC2 cell cultures were measured by ELISA kit from BioLegend. All final reactions were developed with TMB substrate (Thermo scientific ...
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bioRxiv - Immunology 2021Quote: ... fluorochrome-conjugated antibodies (anti-mouse CD45-BioLegend Cat. No. 103126, anti-human CD45-BioLegend Cat. No. 304014, anti-human CD3-BioLegend Cat ...
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bioRxiv - Immunology 2020Quote: ... human Interlukin-6 (IL-6) (100 ng/ml) and human IL-6Rα (100 ng/ml) (BioLegend, San Diego, CA, USA).
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bioRxiv - Cell Biology 2022Quote: ... Cells were incubated with antibodies directly conjugated to fluorescent dyes (anti-human BAFF-R conjugated to FITC, BioLegend, Cat. #: 316904; anti-human BCMA conjugated to APC, BioLegend, Cat ...
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bioRxiv - Immunology 2022Quote: The untouched resting B cells were isolated from the human tonsils by depletion of other cells using MojoSort Human B Cell (CD43-) Isolation Kit (BioLegend), whereas the naïve B cells were isolated using the EasySep Human Naïve B cell isolation kit (Stem Cell Technologies) ...
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bioRxiv - Microbiology 2022Quote: ... using 1:1000 of polyclonal rabbit anti-human MCT1 IgG (Cell Signalling) and 1:1000 mouse anti-human CD147 IgG (Biolegend) followed by HRP-conjugated secondary IgG antibody of specific host type ...
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bioRxiv - Microbiology 2024Quote: CD14+ monocytes were isolated from PMBCs of healthy human blood using the MojosortTM Human CD14 Selection Kit (BioLegend, Cat# 480026) To generate monocyte derived dendritic cells (moDCs) ...
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bioRxiv - Immunology 2023Quote: ... THP-1 and SKOV-3 cell lines and primary human T cells were labeled with fluorescent antibody against human CD70 or isotype control (Biolegend) to verify antigen expression as described before35.
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bioRxiv - Immunology 2023Quote: ... Cells were analyzed and sorted for transfection efficiency via flow cytometry analysis using anti-human CD3 APC/Cy7-conjugated antibody and anti-human CD34 PE-conjugated antibody for TCR-expressing cells (BioLegend, 300426 at 10 μg/mL and BioLegend ...