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Citations for BioLegend :
901 - 950 of 7138 citations for Primary Human Melanocyte Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2024Quote: ... Human recombinant NRG1 was purchased from BioLegend. We reconstituted these growth factors & cytokines in sterile 0.1% bovine serum albumin/ phosphate-buffered saline (FGF1-5mM sodium phosphate and FGF2 - 5mM sodium chloride ...
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bioRxiv - Microbiology 2024Quote: ... PacificBlue-conjugated anti-human CD107a (Biolegend, 328623), FITC-conjugated CD56 (Biolegend ...
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bioRxiv - Immunology 2019Quote: ... 0.2% BSA in PBS with 0.02% sodium azide), blocked with human (humans, NHPs) or mouse (mice) TruStain FcX block (Biolegend) for 10 minutes at room temperature with shaking ...
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bioRxiv - Cancer Biology 2022Quote: ... or TFH mimicking signals (including 0.02 μg/mL recombinant human IL-4, Peprotech; 0.05 μg/ml recombinant human IL-21, Peprotech, and 5 μg/mL anti-human CD40, Biolegend). Complete RPMI medium contained RPMI 1640 (Gibco ...
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bioRxiv - Immunology 2020Quote: ... Monoclonal mouse IgG1 anti-human CD64 FITC-conjugated (clone 10.1) and monoclonal mouse IgG2a anti-human CD59 PE-conjugated were from BioLegend, San Diego ...
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bioRxiv - Bioengineering 2022Quote: ... 100 IU/mL recombinant human IL-2 (Proleukin, Prometheus Laboratories) and 5 ng/mL recombinant human IL-7 (BioLegend) at 37C ...
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bioRxiv - Developmental Biology 2024Quote: ... Cells were subsequently stained with CD47-PerCPCy5.5 and CD26-PE antibodies (Anti-human CD47 PerCP/Cy5.5 conjugate, Biolegend, Cat#323110, 1:200; Anti-human CD26 PE conjugate, Biolegend, Cat#302705 ...
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bioRxiv - Immunology 2023Quote: ... Human Th17 differentiation assays were performed similarly except the concentrations of recombinant human IL-6 (30 ng/ml, BioLegend), TGF- β1 (2.25ng/mL) ...
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bioRxiv - Immunology 2023Quote: Activation consisted of 10μg/mL plate-bound anti-human CD3 antibody (300437) and 1μg/mL soluble anti-human CD28 antibody (302902) purchased from BioLegend, USA ...
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bioRxiv - Neuroscience 2020Quote: ... The cells were incubated with primary antibodies diluted in 1 % BSA/0.3 % Triton X- 100/DPBS (mouse anti-HA (BioLegend, 901501; 1:1000), mouse anti-myc (NEB/Cell Signaling ...
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bioRxiv - Immunology 2023Quote: ... cells were incubated at 4degC with the following fluorochrome conjugated primary anti-mouse antibodies for 45 minutes (all Biolegend, San Diego, CA): anti-CD45 PE-CF594 (clone 30-F11) ...
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bioRxiv - Cell Biology 2022Quote: ... Human cell subpopulations were detected in the bone marrow of transplanted mice using the following antibodies: anti-human CD45-APC (Biolegend, 368512), anti-human CD3-Pacific Blue (Biolegend ...
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bioRxiv - Immunology 2021Quote: ... The cells were then stained with PE-conjugated anti-Human IgG Fc antibody (clone M1310G05, BioLegend 410720, San Diego, CA, USA) to detect bound anti-CTLA-4 and with fluorescein isothiocyanate (FITC)-conjugated anti-CD27 (clone O323,
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bioRxiv - Cancer Biology 2021Quote: ... Cells were then incubated with APC-conjugated anti-human HLA antibody (clone W6-32; cat. no. 311410; BioLegend, San Diego, CA). Cells were subsequently analyzed by flow cytometry for GFP cells/DsRed cells (sensitive/resistant ...
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bioRxiv - Cancer Biology 2021Quote: Wildtype RMC219 and RMC2C cells were harvested and 1 x 106 cells were resuspended in buffer A (PBS 1X, EDTA 2mM, inactivated FCS 1%) and 5uL of Human TruStain FcX (Biolegend, 422301) was added for 10 min at room temperature ...
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bioRxiv - Immunology 2022Quote: ... Every 10^6 B cells in 100 μL solution were then stained with 3 μL FITC anti-human CD20 antibody (BioLegend, 302304), 3.5 μL Brilliant Violet 421 anti-human CD27 antibody (BioLegend ...
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bioRxiv - Microbiology 2020Quote: ... Annexin V-APC (e-biosciences, San Diego, CA) and with cell surface markers α-human CD4 PE (Biolegend, San Diego, CA), α-human CD3 PerCP (BD Biosciences ...
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bioRxiv - Immunology 2022Quote: The concentration of IFN-γ was determined in the supernatant of 6×106 cells from each donor (6 healthy donors) using the Human IFN-γ ELISA deluxe set (BioLegend) according to the manufacturer’s protocol.
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bioRxiv - Biophysics 2019Quote: ... siRNA mediated knockdown was further confirmed by imaging cells labelled with Alexa Fluor 488 anti-human FcƐRIɑ antibody AER-37 (CRA-1) (BioLegend Products, Singapore).
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bioRxiv - Immunology 2020Quote: ... TnT cells and Jurkat-derived cell lines were stimulated overnight with either 10 μg ml−1 plate-bound anti-human CD3e antibody (clone OKT3, BioLegend, #317326) or 1X eBioscience Cell Stimulation Cocktail (81 nM PMA ...
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bioRxiv - Cancer Biology 2019Quote: ... Circulating leukemia cells were detected on day 8 by flow cytometry using a human-specific CD45 antibody (#404012, Biolegend, San Diego). Mice were sacrificed upon signs of morbidity.
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bioRxiv - Genetics 2021Quote: ... A antibody panel was used to identify and sort the CD3+CD8+ T cell population: Human TrueStain FcX (Biolegend; Cat# 422302), PE anti-human CD45 (Biolegend ...
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bioRxiv - Immunology 2021Quote: 600 million cells from human tonsils were resuspended in Miltenyi magnetic-activated cell sorting (MACS) buffer and stained with biotinylated anti-human CD19 (clone: HIB19; BioLegend, #555411) on ice for 30 min ...
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bioRxiv - Cell Biology 2021Quote: Analysis of CAR expression on transduced NK92 cells was done by staining with APC-conjugated anti-human CD314 (NKG2D) mAbs and anti-Flag Tag mAbs (Biolegend, USA). NKG2DLs expression in each cancer cell line was stained with recombinant human NKG2D Fc chimera (R&D Systems ...
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bioRxiv - Immunology 2022Quote: ... and of soluble triggering receptor expressed on myeloid cells 2 (sTREM2) and brain-derived neurotrophic factor (BDNF) were assessed using the Human LEGENDplex Multiplex Assay (BioLegend®) was used following manufacturer’s instructions (Lehmann et al ...
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bioRxiv - Immunology 2022Quote: ... CD8 T cells were stimulated in presence of self-made human IL-2 on α-CD3 (5 µg/ml) (145-2C11, BioLegend), α-CD28 (5 µg/ml ...
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bioRxiv - Microbiology 2022Quote: The level of TNF-α protein expression was evaluated after cell transduction with TPA-TNFα in the cultured medium using human TNFα ELISA kit (Biolegend, UK). First ...
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bioRxiv - Immunology 2024Quote: ... Cultured Raji cells and MV-4-11 cells were washed twice with PBS and stained with FITC-conjugated mouse anti-human CD19 (Biolegend, 302205) for 30 mins ...
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bioRxiv - Immunology 2022Quote: ... 200,000 cells were plated in a 96-well round bottom plate and blocked with 1:1000 Human TruStain FcX™ (Biolegend) for 10 minutes ...
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bioRxiv - Microbiology 2023Quote: ... the cells were incubated with Alexa Fluor® 488-labeled anti-human CD69 antibody or an isotype control (1 µg/ml, BioLegend) for 45 min ...
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bioRxiv - Immunology 2022Quote: ... K562 cells were settled with vehicle-treated or Verbenalin-treated NK cells in the presence of Brilliant Violet 421™ anti-human CD107a (LAMP1) antibody (Biolegend) and GolgiStop™ (BD Biosciences) ...
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bioRxiv - Cell Biology 2023Quote: ... cell suspensions were incubated for 20 min on ice with TotalSeq™-C anti-human Hashtag oligos (HTOs) (1:500, Biolegend, 394661 ...
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bioRxiv - Immunology 2023Quote: Antibody levels in the supernatants from B cells that had been stimulated for 6 days were measured using the LEGENDplex™ Human Immunoglobulin Isotyping Panel (6-plex) (Biolegend), a bead-base immunoassay ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were washed in 1X PBS and stained with Alexa Fluor 700 anti-human B2M antibody (BioLegend, 395708; 1:20 dilution) for 20 minutes ...
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bioRxiv - Cell Biology 2023Quote: ... Remaining chemokine levels in the supernatants of cultured cells were measured in triplicate using the commercially available colorimetric LEGEND MAXTM human CCL5 ELISA kit (BioLegend, 440807) according to the manufacturer’s instructions and read with a SpectraMax M5 plate reader (Molecular Devices ...
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bioRxiv - Cell Biology 2023Quote: Lymph nodes were digested as described above and enriched for CD45- stromal cells by negative selection using MojoSort™ Human CD45 Nanobeads (Biolegend). CD45- cell enrichment was performed according to manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2024Quote: Output of CD34+ cells following hematopoietic differentiation of hiPSCs was determined by staining with PE/Dazzle594-labeled anti-human CD34 antibodies (BioLegend, #343534). Prior to addition of antibodies ...
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Cellular sialoglycans are differentially required for endosomal and cell-surface entry of SARS-CoV-2bioRxiv - Microbiology 2024Quote: ... washed cells were resuspended in 100 μL of FACS buffer with 5 μL of PE anti-human TMPRSS2 antibody (BioLegend #378403) per million cells and incubated for 30 min at 4 °C in the dark ...
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bioRxiv - Immunology 2024Quote: ... These cells were stained with a cocktail of mAb conjugated with DNA-barcoded oligonucleotide that are specific for immune cell lineages (TotalSeq™-C Human TBNK Cocktail, BioLegend) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2020Quote: For analysis of LILRB2 expression on marmoset and human monocyte derived macrophages were first blocked with human TruStain FcX Fc blocking solution (Biolegend), then labelled with and without anti-human LILRB2 (5 µg/mL ...
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bioRxiv - Bioengineering 2021Quote: ... Naïve T cell marker staining was performed with APC/Cy7 mouse anti-human CD45RA (#304128) and BV510 mouse anti-human CD45RO (#304232) antibodies from Biolegend.
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bioRxiv - Immunology 2022Quote: ... PBMCs were surface stained with series of antibodies (anti-human CD158 [KIR2DL1/S1/S3/S5]-FITC [BioLegend, Cat#339504], anti-human CD158b [KIR2DL2/L3, NKAT2]-FITC [BioLegend, Cat#312604] ...
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bioRxiv - Immunology 2022Quote: ... PBMC derived T cells were stimulated for five days at 37°C with 1μg/mL anti-CD3 and anti-CD28 and 10ng/mL of recombinant human IL-2 and recombinant human IL-12p70 (BioLegend). On day four of stimulation ...
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bioRxiv - Biophysics 2022Quote: ... Monoclonal antibodies against human Glycophorin AB (CD235ab) (HIR2; catalog number: 306602) and human CD47 (CC2C6; catalog number: 323102) were purchased from BioLegend.
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bioRxiv - Immunology 2021Quote: ... containing anti-CD9 (human HI9a, mouse MZ3), anti-CD63 (human H5C6, mouse NVG-2), anti-CD81 antibodies (human 5a6, mouse Eat-2) from Biolegend, and anti-human ACE2 (R&D Biosystems cat no ...
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bioRxiv - Microbiology 2021Quote: ... Mouse IgG and human IgG were detected with separate secondary antibodies (goat anti-human IgG Fc, goat anti-mouse IgG Fc, BioLegend) at a dilution of 1:4,000 in blocking buffer and PNPP as described above.
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bioRxiv - Immunology 2021Quote: ... fluorochrome-conjugated antibodies (anti-mouse CD45-BioLegend Cat. No. 103126, anti-human CD45-BioLegend Cat. No. 304014, anti-human CD3-BioLegend Cat ...
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bioRxiv - Immunology 2020Quote: ... human Interlukin-6 (IL-6) (100 ng/ml) and human IL-6Rα (100 ng/ml) (BioLegend, San Diego, CA, USA).
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bioRxiv - Cell Biology 2022Quote: ... Cells were incubated with antibodies directly conjugated to fluorescent dyes (anti-human BAFF-R conjugated to FITC, BioLegend, Cat. #: 316904; anti-human BCMA conjugated to APC, BioLegend, Cat ...
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bioRxiv - Microbiology 2024Quote: CD14+ monocytes were isolated from PMBCs of healthy human blood using the MojosortTM Human CD14 Selection Kit (BioLegend, Cat# 480026) To generate monocyte derived dendritic cells (moDCs) ...