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Citations for BioLegend :
901 - 950 of 984 citations for PB Cadherin Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: ... FACS buffer recipe was D-PBS + 5% FBS + 2% BSA + 5 mM EDTA + 50 µg/ml DNase-I with Human Trustain (Biolegend, 422302). All the compensation was done using UltraComp eBeads™ Compensation Beads (Invitrogen ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were permeabilized for intra-cellular staining with a saponin-based buffer (1% saponin, 10% BSA in PBS) and stained with an antibody for Cytochrome C AlexaFluor 647 (Biolegend, 612310) used at 1:2000 dilution and DAPI ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were permeabilized for intra-cellular staining with a saponin-based buffer (1% saponin, 10% BSA in PBS) and stained with an antibody for Cytochrome C AlexaFluor 647 (Biolegend, 612310) used at 1:2000 dilution and DAPI ...
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bioRxiv - Immunology 2022Quote: ... Cells were washed with PBS supplemented with 2% FBS (PBS+) and then stained with 50µl of an antibody cocktail containing 1:50 diluted anti-human CD45-AF700 (BioLegend, clone HI30), 1:50 diluted anti-human CD3-BV605 (BioLegend ...
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bioRxiv - Genomics 2022Quote: ... Cell pellets were resuspended in FACS buffer (PBS with 1% FBS and 1% penicillin-streptomycin) then stained with the following antibodies: PE anti-CD45 (BioLegend #103105); APC anti-CD31 (Invitrogen #17-0311-80) ...
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bioRxiv - Neuroscience 2022Quote: ... Then the live microglial cells were incubated with PBS supplemented with 5% BSA + HLA-DR mouse monoclonal antibodies pre-conjugated with FITC (1:100, BioLegend, Inc.) + Hoechst 33342 (1:5000 ...
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bioRxiv - Immunology 2023Quote: Lung cells prepared as described above were counted and washed with cold PBS twice before staining with 1:200 Zombie Aqua Fixable Viability Dye (BioLegend, 423101) for 15 min at 4 °C ...
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bioRxiv - Microbiology 2023Quote: ... cells were incubated in 100 µl PBS 1X containing 1.5 μL Zombie NIR Fixable Viability kit (BioLegend, San Diego, CA, USA) at 4°C for 30 min ...
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bioRxiv - Microbiology 2023Quote: ... Each well was blocked using 50 μL of FACS buffer (PBS + 5% FBS) + 2.5 μL Fc receptor (BioLegend, Cat No. 422302) block per well and then 150 μL FACS buffer was added and spun down ...
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bioRxiv - Cell Biology 2022Quote: ... The digested tissue was resuspended in 100 μL of 4% FBS in PBS containing an FITC antibody to CD11b (1:100; BioLegend, 101206) and PE antibody to CD45(1:100 ...
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bioRxiv - Immunology 2023Quote: ... resuspended in 1 ml of PBS containing 1 µl live/dead stain (Zombie UV Fixable Viability kit (Biolegend, cat. no. 423107)) and incubated on ice for 30 min ...
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bioRxiv - Cancer Biology 2023Quote: ... single cell suspensions of samples incubated with myeloid antibody cocktail were washed and resuspended in PBS for flow cytometry run whereas lymphoid antibody cocktail-stained cells were incubated overnight with 1 mL perm buffer (Biolegend; 421402) containing 10 μL of anti-FoxP3 (Biolegend ...
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bioRxiv - Microbiology 2023Quote: ... opsonized bacteria were re-suspended in sterile PBS with 2% fetal bovine serum and stained with APC-conjugated anti-human C3b (Biolegend 846106) and FITC-conjugated anti-human C5b-C9 (LSBio C210266 ...
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Fine tuning of CpG spatial distribution with DNA origami for improved therapeutic cancer vaccinationbioRxiv - Synthetic Biology 2023Quote: BMDC or T cell suspensions collected as described above were washed with PBS and stained with Zombie UV viability dye (BioLegend, #423108) and then washed with cell staining buffer (BioLegend ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were washed in 1X PBS and stained with Alexa Fluor 700 anti-human B2M antibody (BioLegend, 395708; 1:20 dilution) for 20 minutes ...
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bioRxiv - Immunology 2023Quote: ... buffer (PBS containing 2% FBS and 2 mM EDTA) and then incubated with human Fc receptor block (1:100 dilution, BioLegend, 422302) for 15 minutes on ice ...
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bioRxiv - Microbiology 2023Quote: ... 1.5 million cells were washed twice with PBS prior to 15 min of staining with the Zombie NIRTM Fixable Viability dye (BioLegend – 423105), followed by 2 washes with FACS Buffer (PBS ...
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bioRxiv - Immunology 2024Quote: ... Cells were then washed with FACS buffer (PBS+ 2% (v/v) FBS with 1mM EDTA) and incubated with a 1:500 dilution of TruStain FCX (Biolegend, 101320) for 5 minutes ...
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bioRxiv - Immunology 2024Quote: ... Cultured Raji cells and MV-4-11 cells were washed twice with PBS and stained with FITC-conjugated mouse anti-human CD19 (Biolegend, 302205) for 30 mins ...
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bioRxiv - Systems Biology 2023Quote: ... Cells were washed twice with PBS and then incubated with fluorophore-conjugated antibodies (CD81 antibody BioLegend 349501 or Cd44 antibody Biolegend 103023) for 1 hour followed by a PBS wash ...
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bioRxiv - Bioengineering 2024Quote: ... samples were washed twice and blocked for 30 minutes with 50 uL FACS buffer (0.5% BSA in PBS) with TruStain FcX solution (0.5 uL/sample) (Cat # 422301, Biolegend, San Diego, CA). Antibodies were then added to 100 uL of BD Brilliant Stain Buffer (Cat # 659611 ...
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bioRxiv - Developmental Biology 2024Quote: ... blocked in 3% BSA/ 5% normal goat serum in PBS for 1h and incubated overnight at 4°C with primary antibodies (β-III tubulin/anti-tuj-1, 1/1000, Biolegend n°801202 ...
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bioRxiv - Immunology 2021Quote: ... Cells were washed with 2% FBS PBS before incubating with a panel of previously titrated 14 barcoded oligo-conjugated antibodies (BioLegend TotalSeq-C), including FITC-anti-CD45 ...
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bioRxiv - Immunology 2021Quote: Single-cell suspensions prepared from lungs were washed twice with PBS prior to incubating with Zombie UV™ Fixable Viability Dye and TruStain FcX™ (clone 93; both from BioLegend) for 15 minutes at room temperature ...
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bioRxiv - Genomics 2019Quote: ... clones that survived after 3-4 months of culture were washed with PBS and stained with FITC anti-HLA-DQ (Biolegend, Clone: HLADQ1) for 30 minutes on ice and cell surface expression assessed by flow cytometry ...
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bioRxiv - Systems Biology 2020Quote: ... up to 1×106 EnSC/100 µL of Magnetic Bead Buffer (0.5% BSA in PBS) were combined with 5 µL/1×106 cells phycoerythrin (PE) conjugated anti-human SUSD2 antibody (BioLegend, London, UK). After 20 min on ice ...
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bioRxiv - Cancer Biology 2020Quote: ... catalog number L100-L115-01) in PBS with 1% BSA and incubated the beads with 3.5 µg/ml biotinylated anti-CD81 antibody (BioLegend, clone Eat-2) at the room temperature for 40 minutes ...
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bioRxiv - Immunology 2022Quote: ... anti-human CD57-PB (BioLegend, Cat#322316], anti-human CD16-V500 [BD Biosciences, Cat#561314], anti-human CD69-PE-Cy7 [BioLegend, Cat#310190]). 5×105 to 1×106 cells were re-suspended in residual buffer and antibody panels added and incubated on ice for 20 to 30 minutes ...
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bioRxiv - Cell Biology 2021Quote: ... and then resuspended in 10% FCS in PBS (100μl/well) containing TruStain FcX™ (anti-mouse CD16/32) antibody (Biolegend; 1:200) and incubated on ice for 20mins ...
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bioRxiv - Immunology 2022Quote: ... Cells were successively stained for 30 minutes on ice with the cocktail of fluorescently conjugated antibodies in 200 μl staining buffer (1X PBS + 2% FBS): 2 μg/ml biotinylated-RBD-Streptavidin-PE and FITC-anti-human IgG antibody (Biolegend, clone: M1310G05) and DAPI ...
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bioRxiv - Immunology 2020Quote: ... the cells were washed with 1 ml of PBS and finally resuspended in 100 µl of FACS buffer (1% BSA in PBS with Ca2+/Mg2+) with anti-HMGB1 antibody (10µg/ml, BioLegend, catalogue number-651402). The cells were incubated at 4 °C for 30 minutes on ice and were washed with 1 ml PBS (containing Ca2+/Mg2+) ...
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bioRxiv - Immunology 2020Quote: ... Unbound antibodies were removed by washing with PBS were added at 5 × 104 cells/well in the presence of 4 μg/mL brefeldin A (Biolegend, Cat # 420601), 5 μg/mL GolgiStop (BD Biosciences ...
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bioRxiv - Immunology 2020Quote: ... Cells were then permeabilised with Saponin 2% in PBS for 20 min at room temperature and then stained in Saponin 2% in PBS with the appropriate antibodies: Th-1 (anti-CD3-BV510 (1:100, Biolegend, Cat#300448), anti-CD4-PE (1:100 ...
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bioRxiv - Cancer Biology 2021Quote: ... 10 μL of CytoC stain buffer (2% Tween20, 10% BSA (w/v) in PBS) + 1:400 Alexa Fluor 647 anti-cytochrome c antibody (Biolegend, cat#612310) + 1:100 DAPI (1 mg/mL ...
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bioRxiv - Immunology 2020Quote: ... Harvested lymphocytes were washed three times with FACS buffer (5% FBS in PBS) and stained for surface markers using: CD19-PE (363003, Biolegend, RRID: AB_2564125), IgD-FITC (348206 ...
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bioRxiv - Bioengineering 2020Quote: ... cells were washed 3x with PBS and incubated with a cocktail of primary antibodies to intracellular compartment markers - clathrin (1:500, Biolegend MMS-423P), caveolin (1:200 ...
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bioRxiv - Immunology 2021Quote: ... Cell suspensions were resuspended in FACS buffer (PBS, 1% FBS, 1 mM EDTA, 0.02% NaN3) and treated with anti-CD16/CD32 (clone 93, Biolegend 101302, 1:100) for 20 min on ice before staining.
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bioRxiv - Immunology 2020Quote: Cells were first incubated in 100 μL of PBS with 2mM EDTA for 15 min with 5μL Fc Block (Human TruStain FcX™, Biolegend, cat #422301) and a 1:500 dilution of Zombie UV viability dye (Biolegend ...
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bioRxiv - Bioengineering 2022Quote: ... Cells were stained for 30min in flow buffer (PBS, 1mM EDTA, 2% FBS) with antibodies against CD8α-FITC (53-6.7; Biolegend unless otherwise noted), CD19-PerCP/Cy5.5 (1D3) ...
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bioRxiv - Cell Biology 2023Quote: ... the pellet was resuspended in PBS and incubated for 30 min at 4 °C with rat FITC-conjugated mAb against CD11b (1:200, BioLegend, CA, USA). After incubation ...
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bioRxiv - Immunology 2023Quote: ... Cells were pelleted down at 1,500 rpm at 4°C for 5 min and cell pellets were resuspended in 100 uL FACS buffer (PBS + 4% FBS +0.5 mM EDTA) containing Fc block (BioLegend, 101302, 1:200). After 15 min incubation at 4°C ...
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bioRxiv - Cancer Biology 2023Quote: Mice were sedated with ketamine and xylazine and then were injected with 2 ug of APC anti-CD45 (2 ug per mouse diluted in 100 uL PBS, Biolegend 30-F11) retro-orbitally ...
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bioRxiv - Microbiology 2023Quote: ... Tissue was rinsed 3 times in 1X PBS and then incubated with 1:5000 dilution rabbit polyclonal anti-Keratin-14 antibody (Biolegend, Clone Poly19053) for 1 hour and then detected with 1:10000 dilution goat anti-Rabbit IgG Secondary Antibody conjugated with Alexa Fluor™ 488 (ThermoFisher Scientific ...
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bioRxiv - Immunology 2023Quote: Single-cell suspensions prepared from lungs were washed twice with PBS prior to incubating with Zombie UV™ Fixable Viability Dye and TruStain FcX™ (clone 93; both from BioLegend) for 15 minutes at room temperature ...
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bioRxiv - Bioengineering 2023Quote: ... and stained with the following antibodies in FACS staining buffer (PBS with 2% Fetal Bovine Serum, 1% Penicillin-Streptomycin): anti-human CD31 (BioLegend, WM59, FITC), anti-human CD45 (BioLegend ...
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bioRxiv - Immunology 2023Quote: ... then were resuspended in FACS (PBS + 3% FBS + 1mM EDTA + 10mM HEPES) supplemented with TruStain FcX (1:50; BioLegend; San Diego, CA) and TruStain Monocyte Blocker (1:20 ...
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bioRxiv - Microbiology 2023Quote: ... Primary antibodies were resuspended in 0.1% Tween-20 in PBS supplemented with 5% BSA at the following dilutions: TUJ1 (BioLegend, 801202, 1:400), SOX2 (Invitrogen ...
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bioRxiv - Immunology 2023Quote: ... cells were resuspended in 90 μl of 1% BSA in PBS and Fc blocked with 10 μl Human Trustain FcX (Biolegend cat. 422302) for 10 minutes on ice then stained on ice for 45 minutes with a pool of 268 antibodies in 100 μl ...
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bioRxiv - Immunology 2023Quote: ... Cells were resuspended in PBS and stained at 4°C with 100 µL 1:500 Zombie Aqua viability dye (BioLegend, Cat# 423102) and 1:200 mouse FC block (TruStain FcX ...
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bioRxiv - Developmental Biology 2023Quote: ... XER mice were injected intraperitoneally at 1000h and 1800h on GD3 with either PBS or 10µg recombinant Lif (554006, BioLegend, San Diego, CA) followed by blue dye injection and dissection at GD4 1200h ...