Labshake search
Citations for BioLegend :
901 - 950 of 4936 citations for Hepatitis E Virus Capsid Protein ORF2 Mouse Fc Tag since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cancer Biology 2022Quote: ... 2% FCS and 0.09% NaN3) and stained with fluorophore-conjugated antibodies (CD146-APC, SHM-57 or P1H12, BioLegend), (EPCAM-FITC ...
-
bioRxiv - Cancer Biology 2023Quote: ... Resuspended cells were then pooled into a single tube and incubated with Tru-Stain Fc blocking solution (Biolegend) for 10 mins at RT ...
-
bioRxiv - Immunology 2023Quote: ... Individual samples were then combined and stained with intracellular antibodies in CyTOF buffer containing Fc receptor blocker (BioLegend) overnight at 4C ...
-
bioRxiv - Immunology 2023Quote: ... Cells were next treated with human 5µl/tube TruStain FcX™ (Fc receptor blocking solution, Cat# 422302, BioLegend) and incubated for 30 min at RT ...
-
bioRxiv - Immunology 2023Quote: ... The cells were washed with cold PBS and PE-labeled anti-human IgG Fc secondary antibody (Biolegend, 41070) was added and incubated for 30 min at 4°C ...
-
bioRxiv - Microbiology 2023Quote: ... 107 cells per sample were then incubated with 10 μl of Fc-blocker (anti-CD16/CD32 antibody, Biolegend) for 30 min ...
-
bioRxiv - Immunology 2023Quote: ... P008_108)52 for 1h at 4°C followed by PE-rat anti-human IgG Fc (1:200, Biolegend) for 45 min at 4°C ...
-
bioRxiv - Immunology 2024Quote: ... Cells were blocked for 15 min with 10 µL of human Fc receptor blocking solution (TruStain FcXTM; BioLegend). To minimize the interaction of polymeric dyes and improve discrete fluorochrome readout ...
-
bioRxiv - Biochemistry 2023Quote: ... Fc-receptors were first neutralized by incubating cells on ice for 5-10 minutes with TruStain FcX (BioLegend) at 2 μg/mL in 100 μL of blocking buffer ...
-
bioRxiv - Immunology 2023Quote: ... to block Fc receptors followed by staining with fluorochrome-conjugated anti–CD11b (1:200 dilution, BioLegend, no. 101226), anti–Ly6C (1:200 dilution ...
-
bioRxiv - Immunology 2023Quote: ... Cells were incubated for 10 min at 4 °C with Fc receptor block (TruStain FcX, BioLegend, 1:10). Cells were washed and incubated in FACS buffer (1xPBS ...
-
bioRxiv - Cancer Biology 2024Quote: ... The pooled barcoded sample was stained for 10 minutes at room temperature with Fc receptor blocking solution (BioLegend) followed by 1h incubation with the antibody staining cocktail at room temprature ...
-
bioRxiv - Immunology 2021Quote: ... then stained with the I-A/E-APC antibody (MHCII) (1:1,200) (eBioscience) and positively selected for I-A/E-positive cells using anti-APC Microbeads (Biolegend). The obtained final cell fraction consisted of total mTECs (CD45-EpCAM+Ly51-I-A/E+).
-
bioRxiv - Biophysics 2020Quote: ... The membrane was probed with an anti-Aβ antibody targeted to residues 1-16 (1:1000, #E 610, Biolegend) and a secondary anti-mouse IgG HRP-conjugated antibody (1:1000 ...
-
bioRxiv - Immunology 2021Quote: ... anti-mouse mouse MHC H-2 hashtag antibodies (Biolegend TotalSeq™) were used to label separate FACS-purified subsets which were washed ...
-
bioRxiv - Microbiology 2021Quote: ... mouse monoclonal α-GFP and/or mouse monoclonal α-RpoA (BioLegend) primary antibodies ...
-
bioRxiv - Immunology 2021Quote: ... and mouse recombinant PE anti-human/mouse TLR2 (clone QA16A01; Biolegend) were used.
-
bioRxiv - Immunology 2021Quote: ... LEGENDPlex assays (Mouse Proinflammatory Chemokine Panel and Mouse Inflammation Panel; BioLegend) were used ...
-
bioRxiv - Cancer Biology 2022Quote: ... a mouse biotinilated anti-mouse CD45 antibody (clone 30-F11, Biolegend) was used ...
-
bioRxiv - Immunology 2024Quote: ... into the mouse tail vein (5µg/25g mouse, Clone 1A8, Biolegend). 48 hours after the first FITC anti-mouse Ly-6G antibody injection ...
-
bioRxiv - Cancer Biology 2023Quote: ... Mouse primary cells were treated with anti-mouse CD16/32 (Biolegend) and stained with fluorescent antibodies ...
-
bioRxiv - Neuroscience 2021Quote: ... Primary antibody incubation (VMAT2, 1:50, EB06558, Everest Biotech, Ramona, USA, HA-tag: 1:50, 682404, Biolegend, San Diego, USA) was performed for 60 min ...
-
bioRxiv - Biophysics 2022Quote: ... Equivalent surface expression level was verified using an Alexa Fluor 488-conjugated anti-HA-tag mAb (clone 16B12, 1:1000 Biolegend). Flow cytometry data were analyzed using FlowJo V10 software.
-
bioRxiv - Microbiology 2021Quote: ... Binding of His-tagged S1-RBD was detected with Alexa Fluor 488 anti-his tag antibody (clone J095G46, Biolegend, UK) 1:5000 in 25 μL PBST + 0.1% BSA per well ...
-
bioRxiv - Immunology 2020Quote: Alexa fluor 488 or Alexa fluor 594 or FITC or Alexa fluor 648 labeled anti-His-tag (MBL or Biolegend),
-
bioRxiv - Genomics 2021Quote: ... Sample from each donor is also stained with unique hash-tag barcoded antibody (Biolegend totalseq-C, cat# 394661, cat# 39466310). After two washes ...
-
bioRxiv - Immunology 2021Quote: ... washed twice in buffer (PBS + 0.5% BSA) and stained with the anti-FLAG-phycoerythrin (PE) antibody (anti-DYKDDDDK Tag, BioLegend 637310) for confirmation of scFv expression and RSV-F protein conjugated with Alexa Fluor 647 (AF647 ...
-
bioRxiv - Immunology 2022Quote: ... Single-cell libraries were prepared using the single Cell 3’ Kit v 3.1 following a modified 3’ v3.1 assay protocol (User Guide CG000206) to concurrently prepare gene expression and TotalSeq A antibody derived tag (ADT) libraries as recommended by BioLegend. 1 ul of 0.2uM ADT Additive Primer (CCTTGGCACCCGAGAATT*C*C ...
-
bioRxiv - Immunology 2023Quote: Single-cell suspensions from the spleen were prepared as described above and splenocytes were exposed to 2-μM Tag-it VioletTM Proliferation and Cell Tracking Dye (Biolegend) diluted in PBS supplemented with 0.1% bovine serum albumin (BSA ...
-
bioRxiv - Immunology 2023Quote: VLP-producing Expi293F cells were extracellularly and intracellularly stained with Allophycocyanin (APC) anti-Flag (DYKDDDDK) tag antibody (1:500) (Biolegend) and intracellularly stained with the Fluorescein isothiocyanate-labeled KC57 (anti-HIV-1 p24 ...
-
bioRxiv - Immunology 2023Quote: ... The hCMV-peptide pool stimulated cells from each donor were washed and individually stained with Cell-hashtag (HTO:Hash Tag Oligo) TotalSeq C antibody (0.5 µg/condition; Biolegend, Table S2B) as per manufacturer’s recommendations for 30 minutes on ice ...
-
bioRxiv - Cell Biology 2023Quote: Apical supernatant from cells at each harvest time point was assessed using LEGENDPlex Human Anti-Virus Response Panel (BioLegend, San Diego, CA, USA), as per the manufacturer’s instructions and using a FACSCanto II flow cytometer (BD Biosciences ...
-
bioRxiv - Immunology 2021Quote: ... containing anti-CD9 (human HI9a, mouse MZ3), anti-CD63 (human H5C6, mouse NVG-2), anti-CD81 antibodies (human 5a6, mouse Eat-2) from Biolegend, and anti-human ACE2 (R&D Biosystems cat no ...
-
bioRxiv - Cell Biology 2020Quote: ... Cells were incubated with Live/Dead Yellow (1:1000) followed by Fc block (1:200; BioLegend, San Diego, CA), and stained with antibody cocktails for 30 minutes on ice ...
-
bioRxiv - Immunology 2020Quote: ... Non-specific binding was blocked by incubating cells with human anti-Fc Receptor antibodies (TrueStain, Biolegend, Cat. No. 101320) or anti-mouse CD16/32 Fc block (Biolegend ...
-
bioRxiv - Immunology 2021Quote: ... Cells were incubated at 4°C for 15 minutes with an Fc receptor blocker (BioLegend TruStain FcX, cat # 422302) according to manufacturer instructions before cocktails of surface antibodies were added for 30 minutes at 4°C ...
-
bioRxiv - Cancer Biology 2021Quote: ... Red blood cells were lysed (15mM NH4Cl + 10mM KHCO3 for 5 min at R.T.) and CD16/32 antibody was used to block the endogenous FC (#101301, Biolegend). Cells were analysed using a BD FACS LSR Fortessa ...
-
bioRxiv - Immunology 2021Quote: ... Siglec-Fc were used at approximately 20 μg/mL and AF647 Goat anti-hIgG (Clone: HP6017, 0.4 μg/mL, Biolegend) were mixed together in a 1:1 volume ratio right before staining ...
-
bioRxiv - Bioengineering 2022Quote: ... low endotoxin (Super affinity) (Acro) followed by a secondary staining with APC conjugated anti-human IgG Fc Antibody (Biolegend) and RFP expression ...
-
bioRxiv - Immunology 2020Quote: ... Blocking of Fc receptors in T2 cells was performed prior to staining using the TruStain FcX reagent (BioLegend, #422301). Staining with peptide-MHC dextramers was performed for 10 min at room temperature (RT) ...
-
BCR analysis of single-sorted, putative IgE+ memory B cells in food allergy: an ephemeral existence?bioRxiv - Immunology 2019Quote: ... In all assays 1 x 106 cells were first incubated with Human TruStain FcX (Fc Receptor Blocking Solution, Biolegend) or anti-human CD32 (FcγRII Blocker ...
-
bioRxiv - Genomics 2021Quote: ... B cells were incubated with viruses for 2 hr and then stained with Human TruStain FcXTM (Fc Block, BioLegend), CD19-APC (clone HIB19 ...
-
bioRxiv - Immunology 2022Quote: All surface staining was done in the presence of Human Fc block and Monocyte Blocker (Biolegend, San Diego, CA). Following staining ...
-
bioRxiv - Immunology 2022Quote: ... Samples were washed in PBS and were incubated at 4°C with CD16/32 Fc block (cat#101302, BioLegend) in PBS for 10 minutes before staining with antibodies (Supplementary Table 1 ...
-
bioRxiv - Immunology 2022Quote: ... low endotoxin (Super affinity) (Aero) followed by a secondary staining with APC conjugated anti-human IgG Fc Antibody (Biolegend). For cytotoxicity assay analysis ...
-
bioRxiv - Immunology 2022Quote: ... before leukocytes were separated using 30 and 70% Percoll gradients and subsequently stained for 30 minutes in FACS buffer (PBS/2.5%FCS/10mM EDTA/0.01% NaN3) with the following primary antibodies: CD45-PacificBlue (Biolegend, 103126), CD19-biotin (Biolegend ...
-
bioRxiv - Immunology 2023Quote: ... CD141 BV605(Clone:M80 Cat:344117) in the presence of human anti-Fc Receptor antibodies (TrueStain; Biolegend; cat. no. 101320). cDCs1 were FACS-sorted as CD45+Lin- (Lin:CD3 ...
-
bioRxiv - Immunology 2023Quote: ... Cells were washed with LunaStain cell staining buffer (Polaris Biology, China) and first stained with Fc block (Biolegend, USA) for 10 min at room temperature ...
-
bioRxiv - Cell Biology 2023Quote: ... approximately 200,000 cells per sample were resuspended in staining buffer (PBS, 2% BSA, and 0.01% Tween) and incubated for 10 minutes with Fc block (TruStain FcX (BioLegend) and FcR blocking reagent (Miltenyi) ...
-
bioRxiv - Immunology 2023Quote: ... cells were stained for sorting in PBS containing 2% FCS at 4 °C with the following antibodies: CD271-PE (1:200; ME20.4, BioLegend), CD235a-PECy7 (1:20 ...