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Citations for BioLegend :
901 - 950 of 1498 citations for 6 chloro 4 methyl benzo b thiophene 3 o since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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Mice generated with induced pluripotent stem cells derived from mucosal-associated invariant T cellsbioRxiv - Immunology 2023Quote: ... CD3+ T cells were purified from C57BL/6 spleen cells with MojoSort mouse CD3 T cell isolation kit (Biolegend, USA), while CD8+T cells were isolated by adding biotin-labeled anti-CD4 antibody into MojoSort mouse CD3 T cell isolation kit ...
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bioRxiv - Physiology 2023Quote: ... IL-6 and IL-10 in the homogenates were measured using LEGENDplexTM Mouse Custom Panel (BioLegend, San Diego, CA, USA). Assay was performed in the Attune NxT flow cytometer (Thermo Fisher) ...
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bioRxiv - Cancer Biology 2023Quote: 1 × 106 target cells (B16-F10 and Hepa1-6) were suspended in 1ml DPBS containing 5 μM Calcein-AM (BioLegend) and incubated at 37℃ for 30 minutes in the dark ...
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bioRxiv - Immunology 2024Quote: ... Flow cytometry analysis of CTLA4-mediated trans-endocytosis was performed as previously described [6] using the True-NuclearTM Transcription Factor Buffer Set (BioLegend) and the following intracellular antibody panel ...
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bioRxiv - Immunology 2021Quote: ... were independently stained with TotalSeq™-B0303 anti-mouse Hashtag 3 Antibody (Biolegend, cat: 155835) according to the manufacturer’s instructions and with fluorophore-conjugated antibodies for cell sorting (anti-CD45.1-PB ...
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bioRxiv - Immunology 2019Quote: ... anti-mouse CD86 (GL1) and anti-mouse CD40 (Clone 3/23) were all from Biolegend.
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bioRxiv - Cancer Biology 2019Quote: ... filtered and resuspended in MEMα medium or FACS buffer containing 3 μg/ml DAPI (BioLegend). Cells were sorted on BD FACSAria1 or FACSAria2 machines and collected in 150 μl Arcturus PicoPure Extraction Buffer ...
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bioRxiv - Immunology 2021Quote: ... 3) mouse anti-human CD196 prelabeled with Alexa Fluor 488 (CCR6 marker; clone G034E3, BioLegend) and 4 ...
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bioRxiv - Immunology 2021Quote: ... αCD366 (Tim-3) BV711(RMT3-23) and αTIGIT PE-Dazzle594 (2G9) were purchased from BioLegend, San Diego ...
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bioRxiv - Immunology 2021Quote: Macrophages (~3 x 106) were stained with anti-F4/80 (rat monoclonal FITC-conjugated, BioLegend) in a 1:200 dilution in FACS buffer for 20 minutes on ice ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were incubated with Brilliant Violet 421 anti-CD56 (clone HCD56, Biolegend, 3 μg/ml), APC anti-NKp30 (clone P30-15 ...
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bioRxiv - Cell Biology 2021Quote: ... were taken on day 1 and 3 and analyzed for TGFβ using ELISA assay (BioLegend) and IFNγ and TNFα using a multiplex-bead based assay (Meso Scale Discovery ...
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bioRxiv - Cancer Biology 2020Quote: Samples were stained with 10X Chromium 3’ mRNA capture compatible TotalSeq-A antibodies (Biolegend, USA). Staining was performed as previously described by Stoeckius et ...
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bioRxiv - Immunology 2021Quote: ... and for 3 h at 37°C with Brefaldin A (10 μg mL-1; Biolegend). Cell surfaces were stained and cells fixed as described above ...
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bioRxiv - Microbiology 2023Quote: ... lysed in 1X red blood cell lysis buffer for 3 minutes (ref no. 420301, BioLegend), and passed successively through 70μm and 30μm strainers (Miltenyi Biotec) ...
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bioRxiv - Pathology 2023Quote: ... and resuspended in 3 ml red blood cell lysis buffer (Biolegend, San Diego, CA, USA) for 90 s to remove the remaining red blood cells and diluted in 9 mL PBS after incubation ...
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bioRxiv - Pathology 2023Quote: ... and suspended in 3 ml red blood cell lysis buffer (Biolegend, San Diego, CA, USA) for 90 s to remove the remaining red blood cells and diluted in 9 mL PBS after incubation ...
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bioRxiv - Cell Biology 2023Quote: ... the cells were incubated with anti-SSEA-3 rat IgM antibody (1:1000, BioLegend, 330302) at 4°C for 1 h following incubation with fluorescein (FITC ...
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bioRxiv - Neuroscience 2021Quote: ... Primary antibodies were applied overnight at 4°C and included SMI31 (Biolegend, 801601), IBA1 (Wako ...
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bioRxiv - Neuroscience 2019Quote: ... were incubated with a combination of 4 µg Tau12 (Biolegend, cat no 8065), 4 µg Tau 5 (Abcam ...
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bioRxiv - Immunology 2019Quote: Neutrophils obtained after 4-day differentiation were washed and labeled using CFSE (BioLegend). Delta T dishes (Bioptech ...
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bioRxiv - Immunology 2021Quote: ... MDM were suspended in 100μL of 4°C annexin V binding buffer (BioLegend) and the two pHrodo loaded sets were stained with 2μL of FITC Annexin V (BioLegend) ...
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bioRxiv - Neuroscience 2021Quote: ... Cells were then fixed in 4% paraformaldehyde and permeabilized using Permeabilization buffer (Biolegend). Intracellular proteins were stained with antibodies against TNF (MP6-XT22 ...
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bioRxiv - Cancer Biology 2020Quote: ... Samples were incubated overnight at 4°C in primary antibody mixtures (HIF1α, Biolegend; EZH2 ...
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bioRxiv - Cancer Biology 2022Quote: ... and incubation with Human TruStain FxC (10 minutes at 4°C; Biolegend, #422302) to block Fc receptor-mediated binding ...
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bioRxiv - Immunology 2020Quote: ... and cultured for 4 days +/- αIL10 antibody +/- isotype control (5μg/ml, both Biolegend). Assessment of the direct effect of IL-10 on human CD4 T cells was performed by plate bound αCD3/28 (1μg/ml ...
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bioRxiv - Neuroscience 2020Quote: ... Remaining volume was incubated at 4°C with anti-HA antibody (Biolegend, #901513) at 1:150 dilution for 4hrs on a gentle spinner ...
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bioRxiv - Immunology 2020Quote: ... cells were fixed for 10 min at 4°C using IC fix (Biolegend) and cells were then incubated in for 20 min at RT in Permeabilization buffer (biolegend) ...
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bioRxiv - Cancer Biology 2022Quote: ... we added 4 μg/ml PE-labeled anti-CD63 antibody (BioLegend, clone H5C6). We imaged the nanowell using Zeiss Axio Observer Z1 microscope equipped with 20x/0.8 NA objectives and a Hamamatsu Orca Flash v2 camera.
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bioRxiv - Developmental Biology 2023Quote: ... additional staining with anti-CD34 (4 µL CD34 APC/Cy7, clone: 581, Biolegend) and anti-CD14 antibodies (3 µL CD14 PE-CF594 ...
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bioRxiv - Immunology 2023Quote: ... 4 µL of cocktail containing PMA/ionomycin + Brefeldin A (Biolegend, San Diego, CA) was added ...
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bioRxiv - Immunology 2024Quote: ... or Th2-polarizing condition (10 ng/mL recombinant mouse IL-4 (#574304, BioLegend), and 10 μg/mL anti-mouse IL-12/23p40 antibody (clone C17.8 ...
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bioRxiv - Immunology 2023Quote: ... Cells were then washed and stained with CTLA-4-PE-Dazzle 594 (Biolegend), Ki67-PerCP-eFluor 710 (Invitrogen) ...
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bioRxiv - Neuroscience 2024Quote: ... at 4°C for 48 hrs: goat anti-mouse Alexa Fluor 488 (Biolegend), goat anti-rabbit Alexa Fluor 594 (Abcam) ...
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bioRxiv - Developmental Biology 2023Quote: ... Embryos were then incubated overnight at 4 °C with Epcam (cat#118202, Biolegend) (1:100 ...
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bioRxiv - Cancer Biology 2019Quote: ... at concentration 2×10^6/ml in IMDM containing 10% FBS and 100U/ml of recombinant mouse IL-2 (BioLegend, CA). Cells were cultured for up to 7 days and used for functional experiments in vitro and in vivo.
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bioRxiv - Microbiology 2021Quote: ... and resuspended in RPMI with 10% FBS medium and were stimulated for 6 h with a cell activation cocktail containing PMA and ionomycin (Biolegend, # 423302) in the presence of protein transport inhibitor ...
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bioRxiv - Immunology 2020Quote: Supernatants of stimulated T cells were harvested on day 6 of culture and analyzed using the LEGENDplex™ human Thelper 1/2/9/17 multiplex kit (BioLegend). Beads were acquired on a BD Canto II (BD Bioscience ...
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bioRxiv - Developmental Biology 2021Quote: ... Surface staining was done by adding the flow cytometry antibody cocktail outlined in Extended Data Table 6 containing F4/80 (BioLegend, 123120), CD11b (BioLegend ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were induced to differentiate by culturing in the absence of β-Estradiol for 6 days before cells were stained for CD11b (Biolegend) and analyzed on the LSRII flow cytometer (BD Biosciences) ...
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bioRxiv - Microbiology 2023Quote: ... and subsequently with biotinylated α- CADM1 antibody (MBL, Cat. No. CM004-6) and streptavidin-conjugated secondary antibody (BioLegend, Cat. No. 405226). Cells were resuspended in 4◦C PBS 2% FCS before cell sorting.
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bioRxiv - Cancer Biology 2023Quote: For inflammatory activation colon fibroblasts were plated in 6-wells and after 24 hours treated with 1 ng/ml murine recombinant IL-1α (Biolegend, 575002) in PBS for the indicated time periods ...
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bioRxiv - Cancer Biology 2023Quote: Plasma Ig were analysed using the LEGENDplex Mouse Immunoglobulin Isotyping panel (6-plex) by flow cytometry (Cat #740493, BioLegend, CA, USA). Plasma sample and standards was prepared using assay buffer as per the manufacturer’s instruction ...
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bioRxiv - Physiology 2023Quote: The levels of TNF-α and IL-6 in the serum and the supernatant of the crushed muscle tissues were measured using a commercial kit (mouse TNF-α and IL-6 ELISA max standard set, BioLegend). The values of TA and GA muscles were revised to the protein concentration measured by BCA kit and expressed as pg/mg.
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bioRxiv - Immunology 2023Quote: ... CD3+ T cells were isolated from spleens of 6-to-8-week-old OT-II mice by MojoSort mouse CD3+ T cell isolation kit (Biolegend 480024). Stimulated FLS were washed with PBS and co-cultured with CD3+ T cells at a concentration of 5-10×105 cells/mL in 10% RPMI for 5 days with either the cognate OVA323-339 antigen (2μg/mL ...
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bioRxiv - Immunology 2020Quote: ... BMDCs were harvested 3 days later and stained with antibodies specific for CD11c (clone N418, BioLegend) and MHC-I (H-2Kb ...
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bioRxiv - Immunology 2021Quote: ... Live bone marrow cells were subject to FcR-receptor blocking (1:3; BioLegend; Cat. No. 101319) and viability staining (1:500 ...
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bioRxiv - Physiology 2020Quote: ... Live bone marrow cells were subject to FcR-receptor blocking (1:3; BioLegend; Cat. No. 101319) and viability staining (1:500 ...
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bioRxiv - Immunology 2019Quote: ... 3 sections per well) with Alexa647-conjugated anti-EpCAM (1/160 dilution; clone G8.8, 118212, Biolegend), eFluor660-conjugated anti-E-cadherin (1/200 dilution ...
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bioRxiv - Genomics 2019Quote: ... Stained cells were washed thrice with Flow buffer and 7-AAD viability dye (3-5µl, BioLegend) was added before quantification ...