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Citations for BioLegend :
901 - 950 of 2141 citations for 6 FLUORO 2 METHYL 3 4 PIPERIDINE 1H INDOLE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... B cells were stimulated in vitro with anti-CD40 antibody (clone HM40-3, Biolegend) overnight to fully activate HEL+ B cells ...
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bioRxiv - Immunology 2023Quote: ... cells were incubated with 200 µg/mL recombinant Galectin-1 and Galectin-3 (Biolegend). Galectin binding was competitively inhibited by adding 50 mM lactose during the staining procedure.
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bioRxiv - Neuroscience 2023Quote: ... and Purified anti-Tubulin β 3 (TUBB3) Antibody (#801201, RRID:AB_2313773; BioLegend, San Diego, CA).
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bioRxiv - Immunology 2019Quote: ... Plasma IL-6 levels were determined by sandwich enzyme-linked immunosorbent assay (ELISA) (Biolegend ELISA MAX Deluxe, London UK). Values below the blank were reported at the limit of detection for statistical purposes ...
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bioRxiv - Microbiology 2020Quote: ... cells were counted and plated on 6 well plates in RPMI + 5% FBS + 20 ng/ml GM-CSF (BioLegend). Cells were maintained at 37° C 5% CO2 and supplemented on day 3 ...
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bioRxiv - Genetics 2022Quote: ... two-week C57BL/6 c-Kit+ bone marrow cells were cultured in media with recombinant mouse interferon alpha (Biolegend) as indicated with media changes every 2-3 days ...
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bioRxiv - Immunology 2019Quote: TNF-α and IL-6 were measured in the media of activated BMDMs using ELISA kits purchased from Biolegend per manufacturer’s protocol ...
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bioRxiv - Immunology 2021Quote: ... according to the manufacturer’s instructions and stained with PE anti-IL-6 and PE-Cy7 anti-IL-10 (JES3-9D7) Abs (Biolegend).
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bioRxiv - Immunology 2021Quote: T cells were enriched from single cell suspensions of splenocytes isolated from naïve C57BL/6 mice as for flow cytometry using a mouse CD3 T cell negative selection kit (Biolegend). Enriched CD3+ T cells were cell trace stained and cultured at 1x106 cells/mL and stimulated with mouse T-activator anti-CD3/CD28 Dynabeads (ThermoFisher ...
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bioRxiv - Immunology 2021Quote: Human and mouse IL-6 and TNF-α protein were measured by an enzyme-linked immunosorbent assay (ELISA) (BioLegend) according to the manufacturer’s instructions.
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bioRxiv - Immunology 2022Quote: ... For intracellular staining cells were either permeabilized with 0.5% Saponin or 0.1% Triton and stained for TNF-α and/or IL-6 (BioLegend). Cells were washed with 2% serum supplemented 1X PBS and subsequently fixed with 1% PFA ...
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bioRxiv - Cancer Biology 2022Quote: Cells cultured in 2D were seeded at 5 × 105 cells/well in 6-well plates and incubated with recombinant human interferon gamma (IFNγ) (0.5 ng/mL) (Biolegend) and/or recombinant tumour necrosis factor alpha (TNFα ...
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bioRxiv - Immunology 2022Quote: ... male mice were isolated 6 dpi with NP-OVA/alum and stained with anti-CD4 Alexa Fluor 647 (GK1.5, BioLegend), anti-TCRβ PE-Cy7 (H57-597 ...
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bioRxiv - Biochemistry 2024Quote: ... the BMMs were seeded into 6-well plates and incubated with macrophage colony-stimulating factor (M-CSF) (20_ng/ml, Biolegend) and Receptor activator of nuclear factor kappa-Β ligand (RANKL ...
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bioRxiv - Immunology 2023Quote: ... Human Th17 differentiation assays were performed similarly except the concentrations of recombinant human IL-6 (30 ng/ml, BioLegend), TGF- β1 (2.25ng/mL) ...
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bioRxiv - Immunology 2023Quote: Appropriate ELISA kits were used to quantify human or murine IL-6 and/or TNF concentrations according to manufacturer’s recommendations (Biolegend). The 450 nm absorbance was measured by a SpectraMax® plate reader.
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bioRxiv - Immunology 2023Quote: IL-6 secretion was quantified using an ELISA MAX Deluxe Set Mouse IL-6 according to the manufacturer’s instructions (431304, Biolegend). Total and NP-specific antibody levels and IL-2 secretion were measured by ELISA on half-area 96-well plates (Greiner Bio-One ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were then fixed in 3.7% formaldehyde for 6 min and PM HA-GLUT4-GFP detected by an anti-HA antibody (901503, BioLegend). HA staining was visualized by Cy3-goat anti-mouse secondary antibody (115-165-062 ...
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bioRxiv - Immunology 2023Quote: ... both endometriosis and sham mice were treated with intraperitoneal (i.p.) injections of 1μg of rmIL-23 (n=6/group; 589006, BioLegend, USA), or PBS for controls (n=6/group) ...
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bioRxiv - Cancer Biology 2023Quote: ... Cat #126404), and PE anti-mouse H2-Kb/H2Db antibody (28-8-6, cat#114607) were purchased from BioLegend. Anti-VHL (VHL40 ...
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bioRxiv - Immunology 2021Quote: ... fixed with 4% paraformaldehyde fixation buffer (BioLegend 420801, San Diego, CA, USA) for 20 minutes at room temperature and washed twice more with MACS buffer ...
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bioRxiv - Cell Biology 2021Quote: ... Membranes were then incubated at 4°C overnight with ATF4 antibody (BioLegend) at 1:1000 ...
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bioRxiv - Immunology 2022Quote: ... for surface staining and BV605-anti-IL-4 (504125, BioLegend Europe BV), APC-anti-IL-5 (504306 ...
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bioRxiv - Immunology 2022Quote: ... cells were fixed for 20 min with 4% paraformaldehyde (Fixation buffer; BioLegend). When intracellular staining was performed ...
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bioRxiv - Neuroscience 2021Quote: ... and CD284 (TLR-4, clone SA15-21, PE, 2.5 µg/ml Biolegend). After staining ...
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Sterilizing immunity against SARS-CoV-2 in hamsters conferred by a novel recombinant subunit vaccinebioRxiv - Microbiology 2020Quote: ... and further stained with BV421-conjugated anti-mouse IL-4 (Biolegend, 504127) and ...
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bioRxiv - Immunology 2020Quote: ... Cells were stained at 4 °C with Zombie Violet staining (BioLegend, 423113) to exclude dead cells ...
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bioRxiv - Microbiology 2021Quote: ... cells were fixed with 4% paraformaldhyde and permeabilised with CytoPerm buffer (Biolegend). Cells were analysed on BD LSR Fortessa X-20 cytometer ...
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bioRxiv - Cancer Biology 2020Quote: ... rotating at 4°C with either μ5g IRF3 antibody (BioLegend, clone:12A4A35) or equivalent isotype control conjugated with protein A/G magnetic beads ...
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bioRxiv - Immunology 2023Quote: ... and stained for 30mins at 4°C with: TruStain FcX (Biolegend #422302), CD45-AF647 (clone QA17A19 ...
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bioRxiv - Neuroscience 2024Quote: ... The primary incubation was performed overnight at 4°C (phospho-Ser129, BioLegend Cat # 825701 ...
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bioRxiv - Immunology 2024Quote: ... CD4 (clone RM4-4) and CD8 (clone 53-6.7) were from Biolegend. Cleaved caspase 3-AF647 (Clone D3E9 ...
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bioRxiv - Immunology 2024Quote: ... and 10 μg/mL anti-mouse IL-4 antibody (clone 11B11, BioLegend)) or Th2-polarizing condition (10 ng/mL recombinant mouse IL-4 (#574304 ...
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bioRxiv - Immunology 2023Quote: ... and 10 µg/mL anti-IL-4 (BioLegend, Cat no. 504-102) to achieve Th1 polarization ...
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bioRxiv - Cancer Biology 2022Quote: ... and stained (20 min, 4°C) with B220-PerCP/Cyanine5.5 (#103235; BioLegend) and CD138-PE (#142504 ...
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bioRxiv - Cancer Biology 2023Quote: ... PerCP/Cyanine5.5 anti-mouse CD4 Antibody (BioLegend, catalog #116011, clone RM4-4), PE/Cyanine7 anti-mouse CD8α Antibody (BioLegend ...
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bioRxiv - Immunology 2023Quote: ... and stained for 30mins at 4°C with: TruStain FcX (Biolegend #422302), CD45-BV421 (clone HI30 ...
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bioRxiv - Immunology 2024Quote: ... Standards for both IL-4 and IL-13 were purchased from BioLegend. IL-10 ELISA kit was purchased from BioLegend.
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bioRxiv - Microbiology 2020Quote: ... CD19+ and CD4− CD8− CD19− cells were resuspended in R-10 media (RPMI + 2 mM L-glutamine + 10% FBS + Penicillin/streptomycin) + 20 U/ml IL-2 (Biolegend, https://www.biolegend.com). Primary monocytes were resuspended in R-10 media ...
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bioRxiv - Immunology 2020Quote: ... Culture supernatants thereof were removed and analyzed for IL-2 production as a correlate for T-cell activation using an ELISA MAX mouse IL-2 set (BioLegend, SanDiego, USA).
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bioRxiv - Immunology 2022Quote: ... Cells were successively stained for 30 minutes on ice with the cocktail of fluorescently conjugated antibodies in 200 μl staining buffer (1X PBS + 2% FBS): 2 μg/ml biotinylated-RBD-Streptavidin-PE and FITC-anti-human IgG antibody (Biolegend, clone: M1310G05) and DAPI ...
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bioRxiv - Bioengineering 2024Quote: ... Blocking occurred using 5% BSA in TBST and antibody probing using 2% BSA in TBST at the indicated dilutions: α-pERK1/2 (Biolegend 369502) 5000x ...
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bioRxiv - Cancer Biology 2023Quote: Mice were sedated with ketamine and xylazine and then were injected with 2 ug of APC anti-CD45 (2 ug per mouse diluted in 100 uL PBS, Biolegend 30-F11) retro-orbitally ...
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bioRxiv - Immunology 2023Quote: ... Co-culture supernatant was collected following completion of culture and analyzed for IL-2 using the ELISA MAX™ Deluxe Set for human IL-2 (Biolegend, 431804) at a dilution of 1:5.
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bioRxiv - Immunology 2024Quote: ... cells were sensitized with mouse IgG2a cell-type specific antibodies (HUVEC and THP-1: CD31, HEC7, Invitrogen, 2 μg; Fibroblasts: HLA-A,B,C, W6/32, Biolegend, 2 μg) or a mouse IgG2a isotype control (MG2a-53 ...
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bioRxiv - Cell Biology 2020Quote: ... APC anti-human Nectin-2/CD112 (Clone TX31, Biolegend), APC anti-human PVR/CD155 (Clone SKII.4 ...
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bioRxiv - Immunology 2021Quote: ... mouse anti-human IgD-BV421 (Clone IA6-2, Biolegend) and mouse anti-human PD1-APC (Clone EH1202H7 ...
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bioRxiv - Immunology 2019Quote: ... Recombinant mouse CSF-2 (carrier free) was from BioLegend. L-α-phosphatidylcholine (PC ...
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bioRxiv - Genomics 2021Quote: ... anti-IL-2 (MQ1-17H12; PE Cy-7; Biolegend), and anti-MIP-1β (D21-1351 ...
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bioRxiv - Cell Biology 2021Quote: ... and anti-CD45.1/2 (clones A20 and 104, BioLegend) antibodies for 30 min (PB ...