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Citations for BioLegend :
8951 - 9000 of 9316 citations for 8 11 Methano 10a 3 6a 1 propanyl 3 ylidene 8H indeno 2 1 b azocine 12 14 dione 5 acetyloxy 4 benzoyloxy dodecahydro 1 3 dimethyl 9 methylene 3R 4S 5R 6aR 6bR 8S 10aR 11R 11aS 15S 9CI since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... The supernatant was used to determine the IL-2 secretion using the IL2 Elisa kit (#BioLegend), and the cells were used to determine the protein concentration using BCA assay (#Thermo ...
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bioRxiv - Immunology 2024Quote: ... and then stained 1h in PBS 2% BSA + 10µg/mL 2.4G2 with anti-vimentin AF647 (Biolegend) and counterstained using 500ng/mL DAPI dilactate (Invitrogen) ...
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bioRxiv - Immunology 2021Quote: Mouse B cells were bulk sorted from mixed chimeras into Eppendorf tubes containing cold sterile PBS 10% FCS and incubated for 20 min at 4°C with TotalSeq™ DNA-barcoded anti-mouse ‘Hashing’ antibodies (BioLegend) at a 1/100 final dilution ...
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bioRxiv - Cancer Biology 2019Quote: ... Cell surface staining was done for 30 minutes at 4 degrees with the following antibodies (all from BioLegend, San Diego, CA): CD45 (30-F11) ...
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bioRxiv - Biochemistry 2020Quote: ... cells were incubated for 30 minutes at 4°C in PBS with a fluorochrome-conjugated antibody (mouse monoclonal APC-conjugated anti-CD69 (BioLegend 10909); mouse monoclonal PE-conjugated anti-CD25 (Pharmingen 30795X ...
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bioRxiv - Pathology 2022Quote: ... Cells were washed (PBS; twice), centrifuged (300g; 5min.; 4°C) and stained (10min., RT) with Streptavidin (Alexa Fluor® 647-conjugated, Biolegend, #405237 ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... The culture supernatants of stimulated cells were collected for cytokine (i.e., IL-4 and IFN-γ) assays using ELISA kits (BioLegend, CA).
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bioRxiv - Immunology 2022Quote: ... 5ug/mL) and anti-CD28 (clone 37.51; 2ug/mL) in the presence of IL-4 neutralizing antibody (11B11, BioLegend, 5ug/mL) for 18-20 hours before the addition of cytokines as follows ...
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bioRxiv - Neuroscience 2022Quote: ... The homogenates were first blocked in PBS with 10% rat serum with gentle rotation in a cold room and then stained for 1h protected from light at 4°C with CD206-FITC (cat no. 141704, Biolegend®) + MHCII-PE (cat no ...
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bioRxiv - Immunology 2022Quote: ... prM-DENV2-treated PBMCs were treated (1h, 37 °C) in the presence or absence of anti-TNF-α antibody (4 µg/mL, BioLegend). The TNF-α depleted supernatants were used to stimulate HUVEC for 6 hours and the expression of activation markers were analyzed by flow cytometry ...
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bioRxiv - Genomics 2022Quote: ... we stained cells with CITE-seq and hashing antibodies as previously described 22 (Supplementary Table 4) along with a fluorescent HLA antibody (BioLegend 311415). The 5% lowest expressing HLA cells were selected via FACS and 40,000 cells were loaded onto one 10x 3’ V3 Chromium channel ...
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bioRxiv - Immunology 2020Quote: ... for 10 minutes at 4°C and then incubated for 30 min at room temperature with primary antibodies including CD45-BV785 (BioLegend, 103149) CD11b-PE (BioLegend ...
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bioRxiv - Immunology 2020Quote: ... was incubated with 1-2 spike+ cells for 1 hour at 4°C before adding of 0.02 ug/ml ACE2-mFc followed by FITC conjugated anti-mouse IgG (Biolegend, cat#405305) and the MFI of FITC and positive percentage were collected.
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bioRxiv - Immunology 2020Quote: ... cells were washed with PBS twice before staining with the following antibodies or reagents for another 30 min at 4°C in the dark: Streptavidin-BV650 (Biolegend, UK), IgG (H+L ...
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bioRxiv - Immunology 2021Quote: Leukocytes from whole blood and ETA samples were incubated with oligo-conjugated Ig-A/D/G/M for 10 min at 4°C followed by the addition of Human TruStain FcX™ (BioLegend) and 10 min incubation at RT ...
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bioRxiv - Immunology 2022Quote: ... IL-2 and IL-7 were removed from ILC2 culture and additionally cultured for 4 hr before restimulation with IL-33 (Biolegend, #580508) plus NMU (R&D systems ...
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bioRxiv - Immunology 2022Quote: ... FcBlock was added for 10 min prior to fluorescent antibody staining for surface proteins for 30 min at 4°C using the following antibodies: BV510 anti-mouse CD45 (Biolegend 103137), FITC anti-mouse CD11b (eBioscience 11-0112-86) ...
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bioRxiv - Immunology 2022Quote: ... Cells were then stained with fluorescent antibodies for intracellular proteins for 30 min at 4°C using the following antibodies: PE anti-mouse TNF (Biolegend 506306) and APC anti-mouse IL-6 (Biolegend 503810) ...
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bioRxiv - Immunology 2022Quote: ... Cells were fixed with 4% PFA and stained intracellularly with IFN-γ-BV421 and TNFα-PE diluted in Perm-Wash buffer (Biolegend).
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bioRxiv - Immunology 2024Quote: ... for 4 h and stained for intracellular expression of either IL-4 or FOXP3 after fixation and permeabilization using the Fixation Buffer (BioLegend, # 420801), the Intracellular Staining Perm Wash Buffer (BioLegend ...
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bioRxiv - Immunology 2024Quote: ... cells were co-incubated with human CD16-expressing NK cells (NK92humCD16) for 4 hours at 37°C and the 421™ anti-human CD107a (Biolegend) antibody as a degranulation marker ...
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bioRxiv - Cancer Biology 2024Quote: ... Splenocytes from naive or tumor-bearing AST;Cre-ERT2 mice were mixed with 2×106 C57BL/6 splenocytes and incubated in RPMI-10 for 4 hours at 37°C in the presence of brefeldin A (Biolegend 420601) and TAG peptide (SAINNYAQKL [0.5 μM] ...
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bioRxiv - Bioengineering 2024Quote: ... for 10 min at room temperature and then stained for 30 min at 4°C with the following antibody cocktail: anti-mouse CD45.1 (Biolegend, cat.: 110735), anti-mouse TER-119 (eBiosciences ...
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bioRxiv - Immunology 2023Quote: ... Reaction was stopped by centrifugation at 4°C and externalized LAMP1 (CD107a) was stained using FITC-coupled anti-LAMP1 antibody (clone 1D4B, BioLegend, #121605) for 20 minutes at 4°C in the dark ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... was used to mark single cells of DMSO treated mice and TotalSeq™-B0304 anti-mouse Hashtag 4 Antibody (Clone M1/42; 30-F11; 155837; BioLegend) was used for aortic cells of TRAF-STOP treated mice ...
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bioRxiv - Cancer Biology 2023Quote: ... 500,000 cells were resuspended in PBS + 1% FBS and spun at 300g for 10 minutes at 4°C and then resuspended in 25 μL staining buffer (BioLegend #420201). 2.5 μL human TruStain FcX (BioLegend #42230 ...
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bioRxiv - Cell Biology 2023Quote: ... Lysates were clarified by centrifugation for 10 minutes at 20,000 Xg at 4 °C and VASP was subjected to immunoprecipitation using either His6- specific epitope tag affinity matrix (Biolegend #900501) or control antibody matrix (Novagen #69026) ...
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bioRxiv - Bioengineering 2023Quote: ... staining was performed for 30 minutes at 4 C with both 70 nM AlexaFluor647-conjugated HER2 and 1ng/μL anti-DYKDDDDK (anti-FLAG) Tag Antibody-PE (Biolegend, 637310). The 5A12 library was first incubated with either 0.5 nM VEGF (Acro ...
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bioRxiv - Immunology 2021Quote: ... lymph node cell IL-1β/IL-23 plus spermidine treatment group (LN sper) with hashtag 5 (BioLegend, Cat# 155809). The five cell populations were mixed and barcoded ...
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bioRxiv - Microbiology 2022Quote: ... the cell culture media was replaced with complete Huh-7.5 media with 5 μg/mL BFA (Brefeldin A, Biolegend) or equal volumes of DMSO (vehicle control ...
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bioRxiv - Immunology 2021Quote: ... for 30 min, washed with FACS buffer (PBS, 5% BSA, 0.05% sodium azide) and stained with anti-mouse IgG (Poly4053; Biolegend), IgA (Clone 11-44-2 ...
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bioRxiv - Immunology 2019Quote: ... or with culture medium only in the presence of 5¼g/ml Brefeldin A (Biolegend, San Diego, CA, USA) before being submitted to the flow cytometry staining process.
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bioRxiv - Cancer Biology 2021Quote: ... Red blood cells were lysed (15mM NH4Cl + 10mM KHCO3 for 5 min at R.T.) and CD16/32 antibody was used to block the endogenous FC (#101301, Biolegend). Cells were analysed using a BD FACS LSR Fortessa ...
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bioRxiv - Immunology 2021Quote: ... the cells were incubated for 5 min on ice with Fc Block (TruStain fcX anti-mouse CD16/32, Biolegend) and washed with 200 μL FACS buffer ...
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bioRxiv - Microbiology 2021Quote: ... resuspended in 100 μl FACS Buffer plus 5 μl TruStain fcX™ (anti-mouse CD16/32) (Biolegend Cat#101320), and incubated for 10 min on ice ...
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bioRxiv - Microbiology 2021Quote: ... Unspecific bindings were blocked by addition of 5 μl TruStain fcX™ (anti-mouse CD16/32) (Biolegend, Cat#101320) and incubation for 10 min on ice ...
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bioRxiv - Cell Biology 2021Quote: ... NK cells were pre-incubated for 20 minutes with anti-CD56 Alexa Fluor 647 (HCD56, Biolegend, 5 μg/ml). For granule polarization assays ...
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bioRxiv - Microbiology 2020Quote: ... cells were counted and plated on 6 well plates in RPMI + 5% FBS + 20 ng/ml GM-CSF (BioLegend). Cells were maintained at 37° C 5% CO2 and supplemented on day 3 ...
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bioRxiv - Pathology 2022Quote: ... cell suspensions were centrifuged again at 300 g for 5 min and then preincubated with 2.5 µg/ml Fc blocker (156604 for mouse, 422302 for human, BioLegend) on ice for 10 min ...
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bioRxiv - Biochemistry 2022Quote: ... per reaction were incubated with 200 μl antibody binding buffer [1X PBS/ 0.02% Tween 20] and 5 μg of anti-FMRP (Biolegend) and anti-FXR1(Santa Cruz Biotechnology #sc-374148) ...
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bioRxiv - Bioengineering 2019Quote: ... Cells were washed with 1X PBS + 5% FBS + 0.1% sodium azide before being incubated for 20 minutes with Zombie Aqua viability stain (Biolegend) at room temperature ...
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bioRxiv - Immunology 2020Quote: ... and kept the remaining wells unstimulated by adding only an equal volume of 2X brefeldin (5 ug/mL, BioLegend). We incubated plates at 37°C for 4 hours.
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bioRxiv - Bioengineering 2020Quote: ... Plates were washed four times 5 min each with PBS plus 0.05% Tween-20 and then blocked with 1x Assay Diluent A (BioLegend) for 1 h at room temperature ...
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bioRxiv - Immunology 2019Quote: ... dissociated GL261 tumors (5 × 106 cells) were pre-incubated with 1.0 μg of TruStain fcX (anti-mouse CD16/32, clone 93; BioLegend) in 100 μL of FACs Buffer (DPBS with 1% BSA ...
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bioRxiv - Cell Biology 2021Quote: ... and 50 μl of conditioned complete medium containing primary antibody at concentrations of 5 μg/ml for CD59 (Biolegend) or 10 μg/ml Alexa Fluor 488–linked transferrin (Molecular Probes ...
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bioRxiv - Immunology 2022Quote: ... Single-cell suspensions were spun down and resuspended in 5 mL of 1x Red Blood Cell Lysis buffer (Biolegend) and incubated on ice for 5 minutes with occasional swirl ...
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bioRxiv - Immunology 2022Quote: Thawed 0.5×106 cells washed 3x at 400xg for 5 minutes at room temperature with 1x staining buffer (Biolegend) and kept on ice during the live dead staining using acridine orange and propidium dye to measure the cell viability before proceeding for 10x experiments ...
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bioRxiv - Immunology 2023Quote: ... 3x105 sorted naïve CD4 T cells were stimulated with plate-bound anti-CD3 (145-2C11, Cytek) (5 μg/ml) and soluble anti-CD28 (clone 37.51, Biolegend) (1 μg/ml ...
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bioRxiv - Developmental Biology 2024Quote: ... 45 µL of cell suspension was transferred to a new 1.5 mL Eppendorf tube (approximately 350,000 cells) and 5 µL of Human TruStain FcX™ Fc Blocking reagent (Biolegend) were added to block unspecific binding of the hashing antibodies ...
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bioRxiv - Cancer Biology 2024Quote: ... The solution was filtered through a 40 μm filter and then placed in 5 mL RBC lysis buffer (BioLegend) for 5 minutes ...