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Citations for BioLegend :
851 - 900 of 4726 citations for Human V Set And Immunoglobulin Domain Containing Protein 2 VSIG2 CLIA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... CD4+ T cells from blood were isolated from healthy donor peripheral blood mononuclear cells (PBMCs) using the BioLegend human CD4+ T cell isolation kit (BioLegend #480010). Purified CD4+ T cells were co-stimulated with plate-bound CD3 antibody (Biolegend #300333 ...
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bioRxiv - Immunology 2021Quote: ... according to the manufacturer’s instructions and naïve CD8+ cells were obtained using the MojoSort Human CD8+ Naïve T Cell Isolation Kit (Biolegend, Cat no.480045). Purity was consistently over >75% CD8+ with no contamination of CD4+ T cells and >80% of CD8+ T cells being naive ...
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bioRxiv - Bioengineering 2021Quote: ... and IL-10 were analyzed using a human COVID-19 Cytokine Storm Panel 1 (13-plex) kit (Cat. No. 741091; BioLegend, USA) according to the manufacturer’s instructions.
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bioRxiv - Cell Biology 2023Quote: ... Remaining chemokine levels in the supernatants of cultured cells were measured in triplicate using the commercially available colorimetric LEGEND MAXTM human CCL5 ELISA kit (BioLegend, 440807) according to the manufacturer’s instructions and read with a SpectraMax M5 plate reader (Molecular Devices ...
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bioRxiv - Microbiology 2020Quote: ... IL-18 and IFN-γ from human PBMCs were measured by LEGENDplex™ Human Inflammation Panel (Biolegend) according to manufacturer’s instructions.
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bioRxiv - Cancer Biology 2022Quote: ... and stained with anti-human CD45 (PE) and anti-human CD117 (APC) (Biolegend, San Diego, CA, USA). CD45-low/CD117+ leukemia cells were FACS sorted (MA900 sorter ...
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bioRxiv - Cancer Biology 2020Quote: ... Human cells were sorted after staining with anti-human specific antibodies directed against EpCAM and CD298 (Biolegend) with simultaneous depletion of mouse cells stained with anti-mouse-specific antibodies directed against CD45 and CD31 (Biolegend).
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bioRxiv - Immunology 2023Quote: ... containing mouse TruStain FcX (BioLegend) and the mouse cell surface protein antibody panel containing 174 oligo-conjugated antibodies (Supplementary Table 1) ...
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bioRxiv - Genomics 2019Quote: ... cells were washed once in PBS and once in Flow buffer (PBS with 2% BSA) and stained with anti-human CD47-APC antibody (clone CC2C6, BioLegend, Cat. #323123/4, RRID: AB_2716202/3) in Flow buffer (20µl antibody/40×106 cells/ml ...
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bioRxiv - Immunology 2022Quote: ... TNF-α and IL-6 protein were quantified using ELISA MAX Deluxe kits (BioLegend, San Diego, CA), according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... Intracellular staining of Ki67 and p21 were carried out using FoxP3 Fix/Perm Buffer set (Biolegend #421403) following the manufacturer’s instructions.
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bioRxiv - Immunology 2020Quote: ... Intranuclear staining of Ki-67 was performed with the True-Nuclear™ Transcription Factor Buffer Set (BioLegend) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... Cells were then fixed (TrueNuclear™ TF fixation and permeabilization buffer set, BioLegend, San Diego, CA, USA) at room temperature for 60 min ...
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Podoplanin expression in fibroblasts determines lymph node architecture and adaptive immune functionbioRxiv - Immunology 2022Quote: ... Cells were permeabilised for 20 minutes at 4°C using the FOXP3 Fix/perm buffer set (Biolegend) containing purified rat IgG2b anti-mouse CD16/CD32 receptor antibody ...
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bioRxiv - Immunology 2022Quote: ... Collected supernatants were assessed for cytokine content using ELISA MAX™ Standard Set Mouse IFN-γ (Biolegend). DCs were analyzed for the expression of CD86 and MHC class II using flow cytometry ...
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bioRxiv - Immunology 2023Quote: ... cells were fixed and permeabilized with a cytokine staining buffer set (Biolegend, Cat#420801 and Cat#421002) for cytokine/chemokine staining and a Foxp3/Transcription Factor Staining Buffer Set (eBioscience/Thermo Fisher Scientific ...
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bioRxiv - Immunology 2023Quote: ... After washing cells were fixed and permeabilized using the True-Nuclear™ Transcription Factor Buffer Set (BioLegend) followed by intracellular staining for 30 minutes at room temperature ...
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bioRxiv - Immunology 2024Quote: ... cells were fixed and permeabilized using the True-Nuclear Transcription Factor Buffer Set (BioLegend Cat. No. 424401) using the manufacturer’s protocol with overnight fixation/permeabilization.
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bioRxiv - Immunology 2024Quote: ... When appropriate NK cells were fixed and permeabilised using the True-Nuclear Transcription Factor Buffer set (BioLegend) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... were stained after cell fixation and permeabilization with True-NuclearTM transcription factor buffer set per protocol (BioLegend). Data were collected on BD LSR Fortessa or Beckman CytoFLEX flow cytometer and analyzed by FlowJo (Tree Star Inc. ...
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bioRxiv - Immunology 2024Quote: ... Zombie-Green/Violet fixable viability dyes and Foxp3 buffer set were purchased from Biolegend (San Diego, CA). Lymphocytes were stained for cell surface markers ...
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bioRxiv - Cancer Biology 2020Quote: Cells were stained with fluorescent conjugates of Annexin V (BioLegend, FITC Annexin V ...
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bioRxiv - Cancer Biology 2022Quote: ... and stained using Annexin V/PI following manufacturer’s protocol (BioLegend). Cells were kept in the dark until assessment and were analyzed by flow cytometer (BDFACSymphony A3 ...
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bioRxiv - Genomics 2020Quote: ... and incubated in 50 μl annexin-V binding buffer (Biolegend) containing FITC-conjugated annexin-V (Biolegend ...
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bioRxiv - Cancer Biology 2019Quote: ... with allophycocyanin (APC)-conjugated Annexin V (1:20; BioLegend, 640920) for 30 min ...
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bioRxiv - Cell Biology 2021Quote: ... Annexin-V /7AAD staining (Biolegend, San Diego, CA, USA: #640930) and pAKTS473 (ThermoFisher ...
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bioRxiv - Microbiology 2020Quote: ... The cells were then stained with FITC annexin V (BioLegend) and PI solution (BioLegend ...
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bioRxiv - Microbiology 2020Quote: ... pH 7.4) in the presence of Annexin V-FITC (Biolegend) at a concentration of 106 cells/ml for 15 minutes ...
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Blunted Fas signaling favors RIPK1-driven neutrophil necroptosis in critically ill COVID-19 patientsbioRxiv - Immunology 2021Quote: ... The wells were washed once with Annexin V buffer (Biolegend) and cells were stained with Annexin V FITC and 7AAD (Biolegend ...
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bioRxiv - Cancer Biology 2021Quote: ... and incubated with Annexin V-APC (BioLegend; 8 ug/mL) to a final concentration of 0.7 µg/mL for 3 h at RT ...
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bioRxiv - Microbiology 2022Quote: ... followed by a wash with annexin V binding buffer (BioLegend). The cells were then stained with 0.5 µg/ml annexin V-PE (BioLegend ...
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bioRxiv - Bioengineering 2023Quote: ... cells were stained for Annexin V-BV510 (1:50, Biolegend) in Annexin V binding buffer (Biolegend ...
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bioRxiv - Cancer Biology 2023Quote: ... Pacific Blue Annexin V (1:50) were purchased from BioLegend. V500 anti-CD45 (clone 2D1 ...
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bioRxiv - Biochemistry 2023Quote: ... and stained using Annexin V/PI following manufacturer’s protocol (Biolegend). Cells were analyzed via flow cytometer (BDFACSymphony A3 ...
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bioRxiv - Cell Biology 2023Quote: ... cells were stained with PI and annexin V (Biolegend #640906) as described below ...
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bioRxiv - Immunology 2024Quote: ... cells were washed with Annexin V Binding Buffer (Biolegend, 422201) and stained for 15 min at RT with annexin V (Biolegend ...
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bioRxiv - Microbiology 2022Quote: Isolated CD4+T cells from healthy donors were incubated with sE2 or sE2F442NYT in the presence of 5µg/ml human anti-CD3 antibody and 1µg/ml human anti-CD28 antibody (BioLegend) for 2 hrs ...
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bioRxiv - Immunology 2023Quote: ... specific antibodies included anti-mouse CD3ε (145-2C11) and CD28 (37.51) or anti-human CD3 antibody (OKT3) and anti-human CD28 (CD28.2) (antibodies from Biolegend). For flow cytometry experiments ...
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bioRxiv - Immunology 2023Quote: ... with a difference that the cells were Fc blocked with both human Fc blocker (Human TruStain FcX, Biolegend) and mouse Fc blocker (TruStain FcX™ PLUS (anti-mouse CD16/32 ...
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bioRxiv - Microbiology 2022Quote: ... Quantification of cytokine and chemokine levels in culture media was performed using the LEGENDPlex kit from BioLegend (human anti-virus response panel), according to the manufacturer’s recommendations.
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bioRxiv - Immunology 2022Quote: ... Supernatants were then evaluated for IFN-γ levels using the Legend Max ELISA kit with pre-coated plates (human IFN-γ) (Biolegend). Surface phenotype of cells that had been removed from the well was determined by flow cytometry using fluorochrome conjugated antibodies specific for 4-1BB or CD69.
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bioRxiv - Immunology 2023Quote: ... we employed the LEGENDplex™ Human Inflammation Panel 1 (13-plex) flow cytometry-based assay kit (#740809, BioLegend, San Diego, CA, USA). This panel allowed us for simultaneous quantification of 13 human inflammatory cytokines and chemokines (IL-1β ...
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bioRxiv - Immunology 2023Quote: ... or phosphorylated ERK1/2 (pERK1/2; Biolegend), cells were stained with surface markers then fixed with Paraformaldehyde 1% ...
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bioRxiv - Cancer Biology 2021Quote: ... incubated with human Fc receptor block (BioLegend), and stained with cell surface antibodies (1:100 anti-CD137-APC [clone 4B4-1 ...
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bioRxiv - Cell Biology 2022Quote: ... and anti-human CD45-APC (Biolegend, 368512). Human cell subpopulations were detected in the bone marrow of transplanted mice using the following antibodies ...
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bioRxiv - Cell Biology 2022Quote: ... anti-human CD34-Alexa 488 (Biolegend, 343518) and anti-human CD235a-APC (Ebioscience ...
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bioRxiv - Cell Biology 2022Quote: ... anti-human CD3-Pacific Blue (Biolegend, 344823), anti-human CD19-PECy7 (Biolegend ...
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bioRxiv - Molecular Biology 2021Quote: ... using the LEGENDplex Human Angiogenesis Panel (BioLegend) bead-based immunoassay ...
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Single-cell analysis of skeletal muscle macrophages reveals age-associated functional subpopulationsbioRxiv - Cell Biology 2022Quote: ... PE anti-mouse/human CD11b Antibody (Biolegend, Cat# 101208 ...
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bioRxiv - Immunology 2021Quote: Cells were incubated with Human FcX (Biolegend) to prevent non-specific antibody binding ...