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Citations for BioLegend :
851 - 900 of 5212 citations for Dengue Virus Serotype 2 NS1 Protein Insect Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pathology 2022Quote: ... Cells were then washed 3 times with cell staining buffer (#420201, BioLegend) followed by resuspension of the cells in DAPI containing FACS buffer ...
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bioRxiv - Immunology 2022Quote: ... cells after cell surface staining were fixed with Fixation Buffer (Biolegend, USA), permeabilised using 1X Click-iT saponin-based permeabilisation and wash reagent (Invitrogen™ ThermoFisher Scientific ...
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bioRxiv - Immunology 2023Quote: ... DLD-1 cells were labeled with CFSE Cell Division Tracker Kit (BioLegend) according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... Isolated cells were incubated with labeled antibodies in Cell Staining Buffer (Biolegend). Dead cells were excluded based on staining with Live/Dead Fixable Viability Dye (FVD-eFluor780 ...
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bioRxiv - Cancer Biology 2024Quote: ... The cells were resuspended in cell staining buffer (BioLegend, Cat. No. 420201) prior to flow cytometry using a MACSQuant Analyzer (Miltenyi Biotec).
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bioRxiv - Cancer Biology 2024Quote: MM.1S cells were washed three times with Cell Staining Buffer (BioLegend) and then fixed in 1x Fixation Buffer (4% paraformaldehyde ...
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bioRxiv - Bioengineering 2021Quote: ... cells were blocked (Biolegend FACS buffer with 0.05% anti-mouse CD16/32 Biolegend ...
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bioRxiv - Immunology 2023Quote: ... Cell counting beads (Biolegend) were added to all samples for absolute cell number measurement by Aria II cell sorter (BD).
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bioRxiv - Immunology 2023Quote: ... Cell counting beads (Biolegend) were added to all samples for absolute cell number measurement ...
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bioRxiv - Cancer Biology 2022Quote: Oligo-labeled antibody staining was done on both melanoblasts and melanom cells using the TotalSeq™-A Antibodies and Cell Hashing with 10× Single Cell 3′ Reagent Kit (Biolegend). The HashTag Oligonucleotidess used for the analysis are mHTO01 ...
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bioRxiv - Cell Biology 2023Quote: ... breast cancer cells and myeloma cells were treated with recombinant hTRAIL (B294007, Biolegend, 1 to 300 ng/ml for different cells) for 3 h in the presence or absence of either HL-III (5 milliunits/ml) ...
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bioRxiv - Molecular Biology 2021Quote: ... and the hybrid protein in the brain was done using these antibodies: α-Aβ (Biolegend clone 6E10 #803017), α-MAP2 (ThermoFisher #MA5-12826) ...
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bioRxiv - Biochemistry 2020Quote: ... Protein bands were either visualized by Comassie stain or by standard Western blot procedure (anti-ubiquitin antibody BioLegend, Cat Number ...
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bioRxiv - Biochemistry 2020Quote: ... bound proteins were stained for 30 minutes with anti-human IgG-APC (clone HP6017, 1/250 dilution; BioLegend) and anti-HIS-FITC (chicken polyclonal ...
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bioRxiv - Immunology 2020Quote: Antibodies and clones reactive against the following proteins were used: CD2 (TS1/8, BV421 or Pacific Blue, Biolegend), CD3 (UCHT1 ...
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bioRxiv - Microbiology 2020Quote: ... rpoS protein was probed by anti-mouse IgG anti-E.coli RNA sigmaS clone:1RS1 antibody (Biolegend; batch-B274469). Protein gel was cut at 50KDa ladder mark ...
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bioRxiv - Immunology 2022Quote: Biotinylated RBD protein (Acro Biosystems, cat# SPD-C82E9) was complexed with fluorochrome-conjugated streptavidin APC (BioLegend, cat# 405207). Lymph node cells from FNA were incubated with the RBD-fluorochrome complex and subsequently stained with aqua live/dead dye (Invitrogen ...
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bioRxiv - Developmental Biology 2021Quote: The following antibodies and dilutions were used: rabbit anti-transformation related protein 63 (P63, 1:250, 619002, Biolegend), rabbit anti-red fluorescent protein (tdT ...
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bioRxiv - Genetics 2023Quote: ... proteins were transferred onto nitrocellulose membranes and probed with a 1:2,000 dilution of anti-RpoS antibody (Biolegend). For secondary antibody ...
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bioRxiv - Cancer Biology 2023Quote: ... and supplemented with sCTLA-4 conditioned media or murine CTLA-4-Fc fusion protein (10 µg/mL, BioLegend). After 4 days ...
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bioRxiv - Immunology 2021Quote: ... Mouse IL-2 was measured in the supernatants by enzyme-linked immunosorbent assay (BioLegend, BD Biosciences).
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bioRxiv - Microbiology 2021Quote: ... and mouse anti-human BST-2 (clone RS38E, PE-Cy7-conjugated; Biolegend, San Diego, CA, USA) were also used as primary antibodies for cell-surface staining ...
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bioRxiv - Cancer Biology 2022Quote: ... TGFß (1 ng/ml; cat#763102) and IL-2 (5 ng/ml, cat# 575402; All Biolegend) were added along with T cell media (RPMI ...
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bioRxiv - Immunology 2020Quote: ... for 30min followed by staining with αLFA1-Alexa Fluor647 (Clone: CBR LFA 1/2, BioLegend, 366311) for 30 minutes ...
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bioRxiv - Immunology 2021Quote: ... the following probes were generated: SARS-CoV-2 RBD-APC/Cy7 (streptavidin-APC/Cy7 from BioLegend), SARS-CoV-2 spike-StreptTactin PE (StrepTactin PE from IBA Lifesciences) ...
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bioRxiv - Immunology 2021Quote: ... the following probes were generated: SARS-CoV-2 RBD-APC/Cy7 (streptavidin-APC/Cy7 from BioLegend), SARS-CoV-2 spike-StreptTactin PE (StrepTactin PE from IBA Lifesciences) ...
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bioRxiv - Immunology 2022Quote: ... the fixed samples were stained with the following antibodies: PE-Cy7-labeled CD63 (NVG-2, BioLegend), Percp-eFluor 710-labeled iNOS (CXNFT ...
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bioRxiv - Molecular Biology 2022Quote: ... Splenocytes were stimulated for 42 hours at 37°C before adding 2 mM monensin (Biolegend #420701) for an additional 6 hours ...
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bioRxiv - Immunology 2020Quote: ... antibody was purchased from BD Biosciences (San Jose, Calif.) and the anti-CD206 antibody (clone 15-2) was purchased from Biolegend. Briefly ...
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bioRxiv - Immunology 2020Quote: ... and IL-2 concentrations were analyzed using the respective LEGEND MAX Human ELISA Kits (BioLegend, USA).
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bioRxiv - Cancer Biology 2021Quote: ... then fixed in 2% PFA and permeabilized using 1X Intracellular Staining Perm Wash Buffer (BioLegend; 421002) prior to intracellular staining with Vimentin (BD Pharmingen ...
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bioRxiv - Immunology 2021Quote: ... anti-CD11c (clone N418, 25-0144-82, eBioscience) and anti-CD18 (clone M18/2, 101407, BioLegend), -CD29 (clone HMβ1-1 ...
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bioRxiv - Immunology 2022Quote: ... were coated with 1 µg/ml anti-CD3 and 2 µg/ml anti-CD28 antibodies (BioLegend) for 2 hours and seeded with 50.000 T cells/well ...
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bioRxiv - Cancer Biology 2022Quote: ... using ELISA MAX Deluxe Set human (IFNγ #430104, IL-2 # 431804, and IL-6 #430504, BioLegend) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... coated flat bottomed 96 well plates with soluble anti-CD28 (2 μg/mL) (ULTRA-LEAF Biolegend), and with or without human recombinant soluble BAFF at 500 ng/mL ...
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bioRxiv - Synthetic Biology 2022Quote: ... mixed to a final concentration of 2 µg/mL or 60 kDa FITC-labeled streptavidin (Biolegend) to a final concentration of 50 µg/mL ...
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bioRxiv - Cell Biology 2023Quote: ... the samples were incubated with 4’,6-diamidino-2-phenylindole (DAPI) (422801, BioLegend, San Diego, CA) for 2 minutes at room temperature ...
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bioRxiv - Immunology 2023Quote: ... and the following antibodies: rabbit monoclonal anti-mouse/human Mac-2 (clone M3/M8; Biolegend #125401), rabbit monoclonal anti-mouse CD4 (clone EPR19514 ...
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bioRxiv - Neuroscience 2023Quote: ... followed by incubation with 2 ug/ml PE/Cyanine7-conjugated anti-mouse TLR4 antibodies (BioLegend, 145407) or 2 ug/ml PE-conjugated anti-mouse TLR2 antibodies (BioLegend ...
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bioRxiv - Microbiology 2023Quote: ... A total of 2 × 105 donor-matched PBMCs or ToMCs were labeled with CFSE (BioLegend #423801), pre-treated with different inhibitors for 30 minutes ...
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bioRxiv - Biochemistry 2023Quote: ... immunoprecipitation was conducted for the supernantant as described above using 2 µg of M3.2 antibody (Biolegend) and 50 µl Protein G sepharose.
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bioRxiv - Immunology 2023Quote: IL-2 concentrations in the supernatant were measured by enzyme-linked immunosorbent assay (ELISA) from BioLegend. For the CRISPR screen ...
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bioRxiv - Microbiology 2023Quote: ... 2 million PBMC of each sample were first blocked with Human TruStain FcX (BioLegend; catalog #422301), then incubated with 40ng of various anti-human hashtag antibodies carrying unique cell barcodes and 12-100ng of various CITE-seq antibodies ...
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bioRxiv - Immunology 2021Quote: ... cells were incubated before cell surface staining with MitoSpy™ Red CMXRos (BioLegend) at a final concentration of 50 nM in RPMI medium at 37°C for 30 min ...
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bioRxiv - Molecular Biology 2020Quote: ... and cell pellets were resuspended in 0.5 ml of cell staining buffer (BioLegend) supplemented with 5 µl of 7AAD per million cells ...
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bioRxiv - Immunology 2019Quote: ... CD4+ cells were first enriched using Mouse CD4 T Cell Isolation Kit (Biolegend) before electronic sorting ...
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bioRxiv - Physiology 2019Quote: ... red blood cells were lysed with 1x red blood cell lysis buffer (BioLegend). For organ harvest ...
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bioRxiv - Cancer Biology 2020Quote: ... single cell suspensions were dissolved in Cell Staining buffer (Biolegend, San Diego, California) and incubated at 4°C for 10 min prior antibody staining.
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bioRxiv - Molecular Biology 2021Quote: ... Cells lysis was stopped by adding 400 μL Cell Staining Buffer (BioLegend, USA). Cells were then centrifuge for 2 min at 2,000 g for 2 min and re-suspend in Cell Staining Buffer ...
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bioRxiv - Immunology 2022Quote: Cells were analyzed for cell-surface markers using fluorophore-conjugated antibodies (BioLegend, eBioscience). Cell surface staining was performed in FACS Buffer (2% FBS and 2 mM EDTA in PBS ...