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Citations for BioLegend :
851 - 900 of 985 citations for DL Lysine 2 15N DiHCl since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... LAMP-1 was stained as described previously for LAMP-2 but using a rat-IgG anti-mouse LAMP-1 and anti-rat-IgG-AlexaFluor647 (Biolegend) [56] ...
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bioRxiv - Immunology 2024Quote: ... and APC anti-ligand (B7-H6: R&D, clone 875001; CD48: Biolegend, clone; BJ40; Nectin-2: Biolegend, clone TX31; LFA-3: Biolegend, cloneTS2/9 ...
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bioRxiv - Immunology 2024Quote: ... IL-2 (anti-human IL-2, PE-Cy7, clone MQ1-17H12), and CD154 (anti-human CD154, APC, clone 24-31) all from Biolegend in Perm wash buffer (PBS + 0.02% NaN3 + 0.5% bovine serum albumin (BSA ...
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bioRxiv - Genomics 2024Quote: ... A Zombie Violet viability staining was performed by resuspending the cells in 1 mL PBS and adding 2 µl of Zombie Violet (1:1000 dilution, Biolegend). The cells were then kept for 10 minutes in the dark ...
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bioRxiv - Immunology 2024Quote: ... samples were stained on ice for 45 minutes in PBS with 2% Newborn Calf Serum and True-Stain Monocyte Blocker (BioLegend) using a combination of the following antibodies ...
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bioRxiv - Immunology 2024Quote: ... samples were incubated overnight at 4°C with combinations of the following antibodies: PE-Cy7-labeled CD63 (NVG-2, BioLegend), AF532-labeled iNOS (CXNFT ...
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bioRxiv - Immunology 2024Quote: ... Alexa Fluor 594 conjugated anti-hCD19 (mouse IgG1, R & D Systems) and Alexa Fluor 647 conjugated anti-human CD340 (erbB2/HER-2, Biolegend) antibodies were used for CEACAM5 ...
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bioRxiv - Immunology 2024Quote: ... 4×106 cells from spleen were plated in a 96-well plate and incubated with SIINFEKL peptide and anti-CD107a for 2 hours at 37°C before Brefeldin A (5.0 mg/mL: Biolegend, 420601) and Monensin protein transport inhibitors (4 pL/6E6 cells ...
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bioRxiv - Immunology 2024Quote: PBMCs (2×107) of HFRS patients were stained with PE-Cy7-labeled anti-human CD3 mAb (Clone 17A2, BioLegend, USA), APC-Cy7-labeled anti-human CD56 mAb (Clone 5.1H11 ...
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bioRxiv - Immunology 2024Quote: Surface staining of lung single cell suspensions was performed in PBS with 2% Newborn Calf Serum on ice for 30 minutes with the following antibodies: TruStain FcX PLUS (S17011E, BioLegend), APC-labeled Ly6G (1A8 ...
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bioRxiv - Neuroscience 2024Quote: ... The cell pellets were resuspended in 100 µL of staining mix (10% FBS in FACS buffer) and 2 µL of allophycocyanin (APC) anti-HA.11 [clone 16B12] (BioLegend). This amount of antibody was empirically tested to yield the highest stain index ...
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bioRxiv - Microbiology 2024Quote: ... in the presence of 10 ng/mL recombinant human IL-2 and 10 ng/mL of recombinant human IL-15 (BioLegend) for 3 days ...
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bioRxiv - Immunology 2024Quote: ... Autologous CD8+ T cells were isolated from PBMCs using EasySep human CD8+ T cell isolation kit (TEMCELL, Cat# 17953) and stained with 2 μM CFSE (BioLegend) in PBS at r.t ...
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bioRxiv - Immunology 2024Quote: ... Specific IgG and IgM antibody levels were measured in culture supernatants by ELISA using LEGEND MAXTM SARS-CoV-2 Spike S1 Human IgG ELISA kit (BioLegend) and LEGEND MAXTM SARS-CoV-2 Spike S1 Human IgM ELISA kit (BioLegend) ...
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bioRxiv - Immunology 2024Quote: ... and fluorochrome-conjugated anti-CD86 and anti-CD32 antibodies in FACS buffer (1%BSA and 2 mM EDTA in PBS) supplemented with True Stain Monocyte Blocker solution (Biolegend) to prevent aspecific binding of fluorochrome-conjugated antibodies to myeloid cells ...
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bioRxiv - Immunology 2024Quote: Cells were collected and washed in FACS buffer (phosphate-buffered saline supplemented with 2% fetal bovine serum) and subsequently stained with the following antibodies purchased from BioLegend ...
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bioRxiv - Molecular Biology 2024Quote: ... The cells were washed with PBS containing 2 mM EDTA and stained with antibodies against CD59 (1:100, anti-human CD59 PE, Biolegend) and C3c (1:25 ...
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bioRxiv - Cell Biology 2024Quote: ... and the supernatant was used for ELISA analysis of IL-2 and latent TGF-beta secretion levels by the kits from Biolegend according to manufacturer’s instructions.
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bioRxiv - Immunology 2020Quote: ... cells were re-stimulated with plate-bound CD3 and soluble CD28 antibodies with the presence of 20 ng/μl IL-2 (Biolegend #589106) for 3 days before infection with HIV-1 reporter viruses ...
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bioRxiv - Immunology 2022Quote: ... and SCT Dd or SCT Dd-CD4 cell surface expression was regularly assessed by flow cytometry (clone 34-5-85 and 34-2-12, Abcam and Biolegend, respectively). Transfectants were FACS-sorted (FACSDiva and FACSARIAIIIU cell sorter ...
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bioRxiv - Immunology 2020Quote: ... Cells were then incubated for 16 hours at 37°C in the presence of 2 uM monensin (Biolegend; San Diego, CA), 5 μg/mL Brefeldin A (Biolegend ...
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bioRxiv - Immunology 2021Quote: ... permeabilized with saponin 0.2% for 20 min and stained with PE-Cy7-anti-IFN-γ mAb and/or PE-anti-IL-10 mAbs (Biolegend). For TF and GzB/PRF staining ...
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bioRxiv - Immunology 2022Quote: ... 0.0032 μg SARS-CoV-2 WT biotinylated RBD protein (His & AVI Tag) conjugated with Brilliant Violet 605 Streptavidin and TotalSeq-C0973 Streptavidin (BioLegend, 405275) and TotalSeq-C0974 Streptavidin(BioLegend ...
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bioRxiv - Neuroscience 2022Quote: ... 2 mice were used whose cells were labelled with Hashtags oligos (HTO) using the Cell Hashing protocol from Biolegend (TotalSeq-A). All the sorted cells were pooled on the same tube and processed in the Chromium Next GEM Chip G using Chromium Next GEM Single Cell 3’ v3.1.
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bioRxiv - Immunology 2022Quote: ... 0.0032 μg SARS-CoV-2 biotinylated RBD protein (His & AVI Tag) (SinoBiological, 40592-V27H-B) conjugated with APC-streptavidin (BioLegend, 405207), and 5 μL 7-AAD (Invitrogen ...
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bioRxiv - Immunology 2022Quote: ... for 30min at 4 °C and intracellular markers (BV421-labeled anti-mouse IFN-γ, Cat# 505830, BioLegend; FITC-labeled anti-mouse IL-2, Cat# 503806, BioLegend; BV711-labeled anti-mouse TNF-α ...
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bioRxiv - Cancer Biology 2022Quote: ... 0.01% 55 mM 2-mercaptoethanol) supplemented with rmGM-CSF (50 ng/mL) and rmFlt3-L (200ng/mL) (Biolegend, San Diego CA). The culture medium was changed on day 9 of culture ...
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bioRxiv - Immunology 2020Quote: ... were added to the tumor cells and incubated for 2 hours with CD20-TCB (200 ng/ml) and +/- αLFA1 antibody (Clone: TS 1/18, 10 μg/ml, BioLegend, 302116). Cells were then gently washed ...
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bioRxiv - Immunology 2020Quote: Supernatants of stimulated T cells were harvested on day 6 of culture and analyzed using the LEGENDplex™ human Thelper 1/2/9/17 multiplex kit (BioLegend). Beads were acquired on a BD Canto II (BD Bioscience ...
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bioRxiv - Immunology 2020Quote: ... CD19-CAR moiety was labeled by AF647-conjugated anti-mouse F(ab’)2 antibody or biotin-conjugated rhCD19-Fc (ACRO Biosystems, USA) with AF647-conjugated streptavidin (Biolegend, USA). ΔLNGFR was labeled with APC-conjugated LNGFR antibody (clone ME20.4-1.H4 ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells were centrifuged at 1800 rpm for 5 mins and then incubated in 2 μg/mL solution of anti-CD11b antibody (Biolegend 101257) and anti-GR1 antibody (Biolegend 108422 ...
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bioRxiv - Immunology 2021Quote: ... Approximately 2 million cells per experimental condition were stained with 1 μg of TotalSeq™ B0301-B0308 anti-mouse Hashtag antibodies (BioLegend) according to the manufacturer’s instructions and pooled together at equal cell numbers ...
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bioRxiv - Developmental Biology 2021Quote: ... and transferred to 2 ml tubes for incubation with the following antibodies diluted in media containing FBS: CD45-PE/Cy7 (BioLegend, 103114), ICAM2-A647 (Life tech ...
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bioRxiv - Cancer Biology 2022Quote: ... FACS buffer recipe was D-PBS + 5% FBS + 2% BSA + 5 mM EDTA + 50 µg/ml DNase-I with Human Trustain (Biolegend, 422302). All the compensation was done using UltraComp eBeads™ Compensation Beads (Invitrogen ...
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bioRxiv - Genomics 2022Quote: ... 0.2×106 naïve CD4+ T cells were cultured in presence of 10 ng/mL of recombinant IL-12 (BioLegend, Cat#577002), 1 ng/mL of recombinant IL-2 (BioLegend ...
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bioRxiv - Immunology 2022Quote: ... and of soluble triggering receptor expressed on myeloid cells 2 (sTREM2) and brain-derived neurotrophic factor (BDNF) were assessed using the Human LEGENDplex Multiplex Assay (BioLegend®) was used following manufacturer’s instructions (Lehmann et al ...
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bioRxiv - Immunology 2022Quote: ... CD8 T cells were stimulated in presence of self-made human IL-2 on α-CD3 (5 µg/ml) (145-2C11, BioLegend), α-CD28 (5 µg/ml ...
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bioRxiv - Immunology 2022Quote: ... Sixteen micron sections were cut and blocked in .01% Triton X 100 with 2% of FBS and donkey serum including TrueStain Monocyte Blocker (BioLegend 426101) for 1h and then stained overnight with the following directly conjugated mAbs ...
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bioRxiv - Immunology 2024Quote: ... Cells were then washed with FACS buffer (PBS+ 2% (v/v) FBS with 1mM EDTA) and incubated with a 1:500 dilution of TruStain FCX (Biolegend, 101320) for 5 minutes ...
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bioRxiv - Immunology 2024Quote: ... the harvested cells were restimulated using a pool of overlapping Wuhan Spike peptides (Miltenyi, 130-126-701) at 2 µg/mL for 16 hours in the presence of brefeldin A (Biolegend, #400602) at 5 µg/mL ...
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bioRxiv - Cell Biology 2024Quote: ... and the resulting suspension was plated at 1-2×106 cells/ml in complete medium containing 20% FCS and 20 ng/ml M-CSF (BioLegend, 574804). Cells were incubated for 6 days with a medium replacement at day 3 ...
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bioRxiv - Immunology 2023Quote: ... Cells were pelleted at 500xg for 5 minutes and then resuspended in 50uL of PBS+ 2% HI-FBS + 2mM EDTA in the presence of Fc receptor blocking antibody (BioLegend, 101302). Cells were labelled with antibodies as described below ...
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bioRxiv - Immunology 2023Quote: ... Cells were stained with Zombie-violet or Zombie-NIR in PBS at 1:2000 dilution for 5 minutes at room temperature prior to resuspending in PBS + 2% HI-FBS + 2mM EDTA in the presence of Fc receptor blocking antibody (BioLegend, 101302) for blocking ...
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bioRxiv - Immunology 2023Quote: ... Skin immune cells were subsequently added to each fibroblasts-placed well and co-cultured in the presence of 20 ng/ml human IL-2 recombinant and 20 ng/ml human IL-15 recombinant (BioLegend, 570304) in 24 well plates (Ratio of 50:1 immune cell-to-fibroblast) ...
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bioRxiv - Cancer Biology 2023Quote: ... and IL-2 were investigated with an enzyme-linked immunosorbent assay (ELISA MAXTM Deluxe Set, Biolegend®, San Diego, CA, USA) according to the manufacturer’s instruction ...
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bioRxiv - Bioengineering 2023Quote: THP-1 cells were incubated with 2 µg/ml of anti-CD44 antibodies (clone: C44Mab-5; Biolegend, San Diego, CA, USA) for 20 minutes under standard cell culture conditions (95% humidity and 5% CO2 at 37°C ...
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bioRxiv - Immunology 2023Quote: Levels of CXCL9 and CXCL10 were measured in plasma (diluted 2-fold) using the mouse cytokine release syndrome panel LEGENDplex system (BioLegend, UK), according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: Splenocytes from intramuscularly vaccinated mice were incubated in culture with a pool of 253 overlapping 15-mer SARS-CoV-2 S peptides(25) for 12 h at 37°C before a 4 h treatment with brefeldin A (BioLegend, 420601). Following blocking with an anti-FcψR antibody (BioLegend ...
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bioRxiv - Bioengineering 2023Quote: ... Nanovials were reconstituted at a ten-fold dilution in Washing Buffer containing 2 μL of 100 μg/mL anti-HLA-A2 FITC antibody (Biolegend, 343304) and incubated for 30 minutes at 37°C ...
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bioRxiv - Immunology 2023Quote: ... we incubated sequential de-paraffinized sections with anti-2’,3’-cyclic-nucleotide 3’-phosphodiesterase (CNPase) (mouse CNPase: 1:200 dilution; Biolegend, Inc.), anti-CD3 (rabbit ...