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Citations for BioLegend :
851 - 900 of 1305 citations for 6 chloro 5 methoxy 3 Pyridinecarboxylicacid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... resuspended in 50 µL FACS containing 5 µL human Fc-block (Biolegend, 422302), and blocked for 15 min at RT ...
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bioRxiv - Immunology 2023Quote: ... 5 μg of fluorescently conjugated anti-CD31 (clone 390, BioLegend; either Alexa 594) and anti-CD49b antibodies (clone HMa2 ...
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bioRxiv - Immunology 2023Quote: ... and stained with a viability dye (NIR Fixable Viability Kit (42301/5, BioLegend) or LIVE/DEAD Fixable Near-IR Dead Cell Stain Kit (L34975 ...
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bioRxiv - Immunology 2023Quote: ... PE/Cyanine7 anti-mouse CD64 (FcγRI) antibody(1:200, Biolegend, X54-5/7.1), Alexa Fluor 488 or PE–conjugated rat anti-mouse CD31 [platelet endothelial cell adhesion molecule 1 (PECAM1)] antibody (1:200 ...
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bioRxiv - Microbiology 2023Quote: ... Cells were incubated for 5 min with anti-mouse CD16/32 Ab (BioLegend) to block Fc receptors ...
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bioRxiv - Cell Biology 2023Quote: ... cells were sorted for highest 5% GFP expression using Zombie Aqua (BioLegend 423101) and the MoFlo Astrios (Beckman Coulter).
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bioRxiv - Immunology 2024Quote: ... Cells were stained with BV421-conjugated anti-CD64 (BioLegend, clone X54-5/7.1) and acquired on a BD LSRII ...
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bioRxiv - Immunology 2024Quote: ... according to manufacturer instructions and labeled with 5 M Tag-it Violet (Biolegend) for 20 minutes at 37°C ...
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bioRxiv - Immunology 2024Quote: ... along with the addition of 5 μg/ml anti-CD28 antibody (Biolegend 102122) for a duration of 2 days ...
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bioRxiv - Physiology 2024Quote: ... mouse anti-mouse CD64-PE/Dazzle594 (clone X54-5/7.1, cat# 139320, BioLegend), rat anti-mouse PDGFRα-PE/Cy5 (clone APA5 ...
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bioRxiv - Cancer Biology 2023Quote: ... 1/500 dilution of anti-Keratin 5 (PRB-160P, BioLegend, Dedham, MA, USA); 1/300 dilution of anti-CC10 T-18 (sc-9772 ...
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bioRxiv - Cancer Biology 2019Quote: ... washed once with PBS at concentration 2×10^6/mL in RPMI1640 containing 10% FBS and 100U/ml of recombinant human IL-2 (BioLegend, San Diego, CA). After 72h ...
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bioRxiv - Immunology 2022Quote: Whole PBMCs were seeded at 1-2×10^6 cells per well in 48-well plates in the presence of 200ng/ml soluble anti-CD3 (OKT3 clone, Biolegend, San Diego, CA) and 10ng/ml IL-2 (Peprotech ...
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bioRxiv - Immunology 2020Quote: ... DCs were isolated from spleens and lymph nodes of C57BL/6 mice using a MojoSort™ Mouse Pan Dendritic Cell Isolation Kit (BioLegend cat. 480097). DCs were cultured with supernatants of necrotic keratinocytes generated by an overnight culture of UV-irradiated (2 J/m2/s for 40 min ...
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bioRxiv - Immunology 2020Quote: Concentrations of IL-1β and IL-6 in cell culture supernatants or blood serum were measured using cytokine-specific ELISAs (BioLegend ELISA Max kits), according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: Serum samples were collected three hours after the first STING agonist injection and analyzed for inflammatory cytokines (IFN-α, IFN-ß, TNF-α and IL-6) using a LEGENDplex Mouse Inflammation Panel (BioLegend). For cell culture supernatants ...
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bioRxiv - Cancer Biology 2022Quote: CAR-T cells were co-cultured with tumor at ratio of 2:1 for 6 hours at 37o C in CO2 incubator with CD107a antibody (Biolegend, 328620, clone-H4A3) and golgistop (BD Biosciences ...
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bioRxiv - Immunology 2023Quote: Purified and biotinylated IL-6 (504502 and 504601, respectively) and IL-10 (505002 and 504906, respectively) antibodies were purchased from Biolegend (San Diego, CA). Purified antibodies were used to coat an ELISA plate ...
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bioRxiv - Immunology 2024Quote: NK cells were enriched from splenocytes from WT or Pvrig KO C57BL/6 mice by using the MojoSort™ Mouse NK Cell Isolation Kit (Biolegend, cat. 480049). Enriched NK cells were cultured and stimulated in vitro in RPMI-1640 Medium (ATCC ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: Determination of the cytokine levels in the supernatants was carried out using commercial ELISA kits for human IL-6 and TNF (BioLegend, San Diego, US). ELISA was carried out according to the manufacturer’s instructions.
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bioRxiv - Microbiology 2020Quote: ... The blot was then washed 3 times in PBS-T and probed with goat anti-rat HRP (Biolegend) and goat anti-mouse HRP (Biolegend ...
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bioRxiv - Immunology 2022Quote: ... 96-well ELISA plates were coated with 3 μg/ml GPI-RBD-GM-CSF in coating buffer (BioLegend) overnight at 4 °C ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were washed 3 times in FACS buffer and incubated in Cyto-Fast Fix/Perm Buffer (BioLegend, 426803) for 20 minutes at room temperature ...
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bioRxiv - Immunology 2020Quote: ... frozen PBMC were thawed and stained with 3 μl of anti-human CD5-APCeF780 antibody (clone L17F12, Biolegend), 2 μl of anti-human CD19-PECy7 antibody (clone HIB19 ...
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bioRxiv - Immunology 2021Quote: ... The first surface marker panel included: BV421 and PerCP/Cy5.5 anti-TIM-3 (clones B8.2C12 and RMT3-23, BioLegend), BV510 anti-PD-1 (EH12.1) ...
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bioRxiv - Physiology 2022Quote: ... 3 106 cells were then incubated with Fc receptor blocking solution (Human Trustain FcX, Biolegend, San Diego, USA) for 5 minutes at 4°C ...
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bioRxiv - Cancer Biology 2022Quote: ... 3× before being incubated with APC-conjugated rat anti-CD83 antibody (1.25 μL/10×106 cells; Biolegend, 121510) for 30 minutes on ice ...
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bioRxiv - Cell Biology 2019Quote: Panel 3 (fresh in vivo tissue): anti-CD45-Brilliant Violet 570 (HI30 clone, 304034, BioLegend, dilution 1:50), anti-CD235a-Pacific Blue (HI264 clone ...
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bioRxiv - Neuroscience 2022Quote: ... and 3 washes were performed prior to secondary antibody staining with anti-rabbit bv421 1:500 (BioLegend 406410) with 3 additional washes after secondary antibody ...
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bioRxiv - Immunology 2022Quote: ... 3-4 × 106 splenocytes after cell surface staining were fixed with Cyto-Fast Fix/Perm buffer (BioLegend, USA) for 20 min at room temperature and washed twice with FACS staining buffer ...
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bioRxiv - Cell Biology 2022Quote: ... Both cell fractions were stained with cell hashing antibodies (1:1000, #2 for heart, #3 for blood, Biolegend) and Propidium iodide (1:100 ...
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bioRxiv - Immunology 2023Quote: ... Cells were plated in a 96-well round-bottom plate coated with 3 μg/ml anti-CD3 (Biolegend), uncoated well or with PMA (1 ng/ml ...
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bioRxiv - Cancer Biology 2023Quote: ... Wells were again washed 3 times before adding 50 µL per well of HRP Streptavidin (#405210, BioLegend, USA) diluted 1:2000 in 1% SureBlock solution and incubated 1 h at RT ...
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bioRxiv - Molecular Biology 2023Quote: ... incubated with secondary antibody diluted in 3% BSA PBS (Alexa Fluor 488 Goat anti-mouse IgG, BioLegend, 405319) in the dark for 1 hour at room temperature and washed 3x with PBST ...
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bioRxiv - Immunology 2024Quote: ... and blocked using 3% FCS or mouse serum in Facs buffer and/or Tru block monocyte blocker (Biolegend) as required ...
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bioRxiv - Synthetic Biology 2024Quote: ... and stained for the intracellular accumulation of IFNγ and TNFα (Supp. Table 3) in 1x Permeabilization Buffer (Biolegend). Samples were analyzed using a Fortessa LSR flow cytometer (BD ...
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bioRxiv - Cancer Biology 2022Quote: ... 96-well plates were coated with 5 g/ml of anti-CD28 antibody (Biolegend) o/n at 4°C to provide a co-stimulatory signal during co-culture ...
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bioRxiv - Neuroscience 2021Quote: ... The cell suspension was preincubated with TruStain FcX Plus Antibody (BioLegend, Supplementary Table 5) on ice for 10 minutes ...
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bioRxiv - Genomics 2020Quote: ... 564219) for 5 minutes and stained with directly conjugated anti-human CD45 (Biolegend 304006) and EPCAM (eBioscience 25-9326-42 ...
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bioRxiv - Immunology 2020Quote: ... PE-Anti-Mouse-CD254-(TRANCE-RANKL)-(IK22/5)-(510005) were acquired from Biolegend (USA).
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bioRxiv - Immunology 2022Quote: ... Cells were than stained with Siglec-5 Fc chimeric protein (Biolegend, San Diego, CA) pre-incubated with anti-human IgG Fc fluorescent antibody (Biolegend ...
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bioRxiv - Pathology 2021Quote: ... mice were injected with 5 μg recombinant mouse IL-10 (Biolegend, San Diego, CA) or sterile saline 24 hours after APAP challenge ...
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bioRxiv - Immunology 2022Quote: ... the following antibodies were used: CD4 PerCP-Cy5.5 (clone RM4-5, 1:100; BioLegend), CD44 APC-Cy7 (clone IM7 ...
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bioRxiv - Microbiology 2019Quote: ... the splenocytes were incubated for 2 h with 5 μg/ml brefeldin A (Biolegend) for intracellular trapping of cytokines and then stained with Zombie Aqua (1:200 ...
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bioRxiv - Cancer Biology 2020Quote: ... cytokeratin 5 (CK5) and 14 (CK14) (904801 and 905501 respectively, BioLegend, San Diego, CA). The tissue sections were counterstained ...
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bioRxiv - Immunology 2020Quote: ... IL-5 and IL-13) using the LEGENDplex mouse Th cytokine 13-plex (Biolegend) kit.
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bioRxiv - Cell Biology 2020Quote: ... Cell pellets were resuspended in 5 ml of red blood cell lysis solution (BioLegend) on ice for 5 min followed by the addition of 20 mL of complete media ...
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bioRxiv - Developmental Biology 2022Quote: ... Naïve hPSCs were passaged by 5 min incubation at 37 °C with Accutase (BioLegend). Primed hPSCs were cultured on plates pre-treated with 5 μg/ml Vitronectin (ThermoFisher Scientific ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were washed in PBS + 5% FBS and fixed in FluoroFix buffer (422101, BioLegend) prior to analysis on the BD LSR II analyser.
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bioRxiv - Cancer Biology 2022Quote: ... Brilliant Violet 421-conjugated rat anti-CCR7 (5 μL/10×106 cells; Biolegend, 120120) and Cells were incubated for 30 minutes on ice ...