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Citations for BioLegend :
851 - 900 of 1726 citations for 6 Hydroxy 2 2 pyridinyl 4 3H pyrimidinone since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... were added to the tumor cells and incubated for 2 hours with CD20-TCB (200 ng/ml) and +/- αLFA1 antibody (Clone: TS 1/18, 10 μg/ml, BioLegend, 302116). Cells were then gently washed ...
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bioRxiv - Immunology 2019Quote: ... PBMCs were then thawed and plated in 24-well plates (2-2.5 millions/1ml/well) pre-coated with anti-CD3 (2.5 ug/ml, HIT3a clone, Biolegend, San Diego, CA) for 24 hours before harvesting ...
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bioRxiv - Immunology 2019Quote: ... Antibody coated cells were mixed with resting NK cells in the presence of 1 μl BV421 anti-CD107a mAb for 2 hr at 37°C (H4A3, Biolegend 328626). Cells were then stained with mAbs to KIR2DS4 (Miltenyi Biotec ...
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bioRxiv - Immunology 2020Quote: ... CD19-CAR moiety was labeled by AF647-conjugated anti-mouse F(ab’)2 antibody or biotin-conjugated rhCD19-Fc (ACRO Biosystems, USA) with AF647-conjugated streptavidin (Biolegend, USA). ΔLNGFR was labeled with APC-conjugated LNGFR antibody (clone ME20.4-1.H4 ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells were centrifuged at 1800 rpm for 5 mins and then incubated in 2 μg/mL solution of anti-CD11b antibody (Biolegend 101257) and anti-GR1 antibody (Biolegend 108422 ...
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bioRxiv - Immunology 2019Quote: ... They were blocked for 1 hour at room temperature in 2% donkey serum and CD16/CD32 (Biolegend, Clone 93, 1:200). Samples were incubated overnight at 4°C in primary antibody diluted in blocking buffer (R&D AF771 goat anti-IL-1R ...
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bioRxiv - Immunology 2021Quote: ... Approximately 2 million cells per experimental condition were stained with 1 μg of TotalSeq™ B0301-B0308 anti-mouse Hashtag antibodies (BioLegend) according to the manufacturer’s instructions and pooled together at equal cell numbers ...
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bioRxiv - Developmental Biology 2021Quote: ... and transferred to 2 ml tubes for incubation with the following antibodies diluted in media containing FBS: CD45-PE/Cy7 (BioLegend, 103114), ICAM2-A647 (Life tech ...
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bioRxiv - Cancer Biology 2022Quote: ... FACS buffer recipe was D-PBS + 5% FBS + 2% BSA + 5 mM EDTA + 50 µg/ml DNase-I with Human Trustain (Biolegend, 422302). All the compensation was done using UltraComp eBeads™ Compensation Beads (Invitrogen ...
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bioRxiv - Genomics 2022Quote: ... 0.2×106 naïve CD4+ T cells were cultured in presence of 10 ng/mL of recombinant IL-12 (BioLegend, Cat#577002), 1 ng/mL of recombinant IL-2 (BioLegend ...
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bioRxiv - Immunology 2022Quote: ... and of soluble triggering receptor expressed on myeloid cells 2 (sTREM2) and brain-derived neurotrophic factor (BDNF) were assessed using the Human LEGENDplex Multiplex Assay (BioLegend®) was used following manufacturer’s instructions (Lehmann et al ...
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bioRxiv - Immunology 2022Quote: ... CD8 T cells were stimulated in presence of self-made human IL-2 on α-CD3 (5 µg/ml) (145-2C11, BioLegend), α-CD28 (5 µg/ml ...
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bioRxiv - Immunology 2022Quote: ... Sixteen micron sections were cut and blocked in .01% Triton X 100 with 2% of FBS and donkey serum including TrueStain Monocyte Blocker (BioLegend 426101) for 1h and then stained overnight with the following directly conjugated mAbs ...
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bioRxiv - Immunology 2023Quote: ... Skin immune cells were subsequently added to each fibroblasts-placed well and co-cultured in the presence of 20 ng/ml human IL-2 recombinant and 20 ng/ml human IL-15 recombinant (BioLegend, 570304) in 24 well plates (Ratio of 50:1 immune cell-to-fibroblast) ...
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bioRxiv - Immunology 2023Quote: ... Cells were pelleted at 500xg for 5 minutes and then resuspended in 50uL of PBS+ 2% HI-FBS + 2mM EDTA in the presence of Fc receptor blocking antibody (BioLegend, 101302). Cells were labelled with antibodies as described below ...
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bioRxiv - Immunology 2023Quote: ... Cells were stained with Zombie-violet or Zombie-NIR in PBS at 1:2000 dilution for 5 minutes at room temperature prior to resuspending in PBS + 2% HI-FBS + 2mM EDTA in the presence of Fc receptor blocking antibody (BioLegend, 101302) for blocking ...
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bioRxiv - Immunology 2023Quote: Levels of CXCL9 and CXCL10 were measured in plasma (diluted 2-fold) using the mouse cytokine release syndrome panel LEGENDplex system (BioLegend, UK), according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... and IL-2 were investigated with an enzyme-linked immunosorbent assay (ELISA MAXTM Deluxe Set, Biolegend®, San Diego, CA, USA) according to the manufacturer’s instruction ...
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bioRxiv - Bioengineering 2023Quote: THP-1 cells were incubated with 2 µg/ml of anti-CD44 antibodies (clone: C44Mab-5; Biolegend, San Diego, CA, USA) for 20 minutes under standard cell culture conditions (95% humidity and 5% CO2 at 37°C ...
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bioRxiv - Bioengineering 2023Quote: ... Nanovials were reconstituted at a ten-fold dilution in Washing Buffer containing 2 μL of 100 μg/mL anti-HLA-A2 FITC antibody (Biolegend, 343304) and incubated for 30 minutes at 37°C ...
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bioRxiv - Immunology 2023Quote: ... we incubated sequential de-paraffinized sections with anti-2’,3’-cyclic-nucleotide 3’-phosphodiesterase (CNPase) (mouse CNPase: 1:200 dilution; Biolegend, Inc.), anti-CD3 (rabbit ...
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bioRxiv - Developmental Biology 2023Quote: ... anti-Neurod1 (1/500, mouse monoclonal, hybridoma) (Kani et al., 2010), anti-paired box 2 [Pax2] (1/700, rabbit polyclonal) (BioLegend, 901001), anti-Foxp1b ...
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bioRxiv - Immunology 2023Quote: ... For the memory B cell EliSpot, PBMC were stimulated with 1 µg/ml R848 (SigmaAldrich, SML0196) and 10 ng/ml recombinant human IL-2 (Biolegend, 791904) for 5 days before harvesting and plating of 50-100.000 cells/well ...
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bioRxiv - Microbiology 2023Quote: ... opsonized bacteria were re-suspended in sterile PBS with 2% fetal bovine serum and stained with APC-conjugated anti-human C3b (Biolegend 846106) and FITC-conjugated anti-human C5b-C9 (LSBio C210266 ...
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bioRxiv - Neuroscience 2023Quote: Endothelial cells isolated from brain from SARS-CoV-2 infected mice were stained with fixable viability dye (Zombie Green, Biolegend 423111) and blocked with Fc receptor blockade (anti-mouse CD16/32 ...
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bioRxiv - Bioengineering 2024Quote: ... supernatant was removed for analysis with ELISA MAX Deluxe Set Human IL-2 and ELISA MAX Deluxe Set Human IFN-γ (BioLegend). Cytotoxicity at the various indicated timepoints was tested using a luciferase-based assay ...
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bioRxiv - Cell Biology 2024Quote: ... and the resulting suspension was plated at 1-2×106 cells/ml in complete medium containing 20% FCS and 20 ng/ml M-CSF (BioLegend, 574804). Cells were incubated for 6 days with a medium replacement at day 3 ...
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bioRxiv - Immunology 2024Quote: ... Cells were then washed with FACS buffer (PBS+ 2% (v/v) FBS with 1mM EDTA) and incubated with a 1:500 dilution of TruStain FCX (Biolegend, 101320) for 5 minutes ...
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bioRxiv - Immunology 2024Quote: ... the harvested cells were restimulated using a pool of overlapping Wuhan Spike peptides (Miltenyi, 130-126-701) at 2 µg/mL for 16 hours in the presence of brefeldin A (Biolegend, #400602) at 5 µg/mL ...
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bioRxiv - Cell Biology 2021Quote: ... Concentrations of tumor necrosis factor-alpha (TNF-α) and interleukin-6 (IL-6) were determined using ELISA kits (430907 and 431307, BioLegend, USA) according to the manufacturer’s instructions.
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bioRxiv - Immunology 2022Quote: ... and Mouse IL-6 ELISA MAXTM Deluxe Set (Biolegend) according to the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2019Quote: ... Figure 6 A: CD326-BV785 (1:100, BioLegend, 9C4), CD235a-FITC (1:100,eBioscience ...
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The tumour microenvironment shapes dendritic cell plasticity in a human organotypic melanoma culturebioRxiv - Immunology 2019Quote: ... and stained with FITC-labelled anti-IL-6 (BioLegend). Acquisition was performed on a FACSVerse flow cytometer (BD Biosciences ...
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bioRxiv - Immunology 2021Quote: ... or 6 μg/mL anti-ICOS (clone C398.4A, Biolegend) in PBS overnight at 4 °C ...
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bioRxiv - Cell Biology 2022Quote: ... clone HI9a (6 µg/mL, BioLegend, San Diego, USA); anti-CD63–APC ...
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bioRxiv - Immunology 2020Quote: ... anti-MHC class I (clone 28-8-6, BioLegend), anti-β-catenin (clone D2U8Y ...
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bioRxiv - Cell Biology 2021Quote: ... and CD309/Kdr/Flk-1 (7D4-6)-APC (BioLegend). 4’ ...
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bioRxiv - Immunology 2022Quote: ... or α-IL-6 (BioLegend, 1:100, MP5-20F3) antibodies for 30 minutes at room temperature ...
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bioRxiv - Biochemistry 2023Quote: IL-6-APC (BioLegend, 504508, MP5-20F3, 1 μL)
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bioRxiv - Bioengineering 2024Quote: ... Magnetic beads displaying recombinant IL-6 protein (BioLegend #570806) were prepared by washing 70 uL of Tosyl-activated M-280 magnetic dynabeads (ThermoFisher #14203) ...
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bioRxiv - Immunology 2023Quote: ... anti-mouse TotalSeq-A Hashtag antibody (1-6; BioLegend), PE-labeled Siglec F (S17007L ...
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bioRxiv - Immunology 2024Quote: ... and cytokine stains with IL-6 (MP5-20F3; Biolegend), IL-10 (JES5-16E3 ...
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bioRxiv - Cell Biology 2023Quote: ... then incubated for 30 minutes at 4°C with pre-chilled 4°C PBS (200 uL) containing 4 uL FITC-conjugated anti-CD63 (clone H5C6, Biolegend) and 4 uL APC conjugated anti-CD9 (clone H19a ...
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bioRxiv - Immunology 2021Quote: ... CD11a (M17/4, Biolegend), CD27 (LG-7F9) ...
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bioRxiv - Immunology 2021Quote: ... and IL-4 (BioLegend) were prepared in PBS at concentrations of 1000 μg/mL ...
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bioRxiv - Immunology 2022Quote: ... CD11a (M17/4; Biolegend), and CD69 (H1.2F3 ...
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bioRxiv - Immunology 2022Quote: ... IL-4 (BioLegend, 574004), IL-13 (BioLegend ...
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bioRxiv - Immunology 2021Quote: ... Cells were washed with 2% FBS PBS before incubating with a panel of previously titrated 14 barcoded oligo-conjugated antibodies (BioLegend TotalSeq-C), including FITC-anti-CD45 ...
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bioRxiv - Immunology 2019Quote: PBMCs were seeded in a 24 well plate at 2 × 106 cells/well in RPMI-1640 with anti-CD107a antibody (Biolegend, 1:200). Then ...
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bioRxiv - Microbiology 2020Quote: ... approximately 2-10 million total human lung cells were resuspended in MACS buffer and blocked with Human TruStain FcX (Biolegend cat# 422032) for 15min at 4°C followed by HTII-280 antibody (Terrace Biotech ...