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Citations for BioLegend :
851 - 900 of 1006 citations for 2 Pyridinesulfonic acid methyl ester since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... were derived from a cell sort that included an additional cell hashing step (TotalSeq-C0251/C0252/C0253/C0254/C0255 anti-human Hashtag 1/2/3/4/5 antibodies, cat # 394661/394663/394665/394667/394669, Biolegend) prior to B cell enrichment and staining ...
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bioRxiv - Immunology 2024Quote: ... The sections were then rinsed in a washing solution twice before nuclei were stained with 4’,6-diamidino-2-phenylidole (DAPI; BioLegend). The sections were rinsed with washing solution one final time and then mounted on slides for examination under an Olympus FV1000 laser-scanning 4-channel confocal microscope (Olympus).
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bioRxiv - Immunology 2024Quote: ... cells were washed with FACS staining buffer (PBS containing 2% fetal bovine serum [FBS] and 1 mM EDTA) and incubated with an Fc receptor blocker (TrueStain fcX; BioLegend) for 5 minutes at 4℃ ...
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bioRxiv - Microbiology 2023Quote: ... BMDMs were stimulated for 2 hours prior to infection with vehicle or 10 ng/mL of each cytokine: GM- CSF (Biolegend), M-CSF (Miltenyi) ...
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bioRxiv - Cancer Biology 2022Quote: ... protein expression (ADT) and hashtag (HTO) libraries were generated using Chromium Single Cell 3’ version 2 reagents (10x genomics and Biolegend) per manufacturers’ protocols ...
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bioRxiv - Cell Biology 2022Quote: ... or P14 bone marrow cells from 2-4 mice per genotype were c-kit selected and stained with tagged Total-Seq B antibodies (Biolegend) to CD150 ...
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bioRxiv - Genomics 2022Quote: ... flat bottom 96-well plates were coated with 2 μg/mL of anti-CD3 (Clone: 145-2C11, BioLegend, Cat#100302) in PBS overnight at 4°C or 4 hrs at 37°C ...
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bioRxiv - Immunology 2022Quote: ... 100μg/ml streptomycin and 2 mM EDTA) and stained for 20 minutes at 4 degrees with the following fluorescently conjugated antibodies (BioLegend): anti-CD11c (clone N418) ...
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bioRxiv - Immunology 2022Quote: ... mice were inoculated with CEA10-mRFP FLARE conidia and lung cells were incubated with 0.05 mg/ml of pHrodo-Zymosan at 37 °C for 2 hours and stained with Survival dye (efluor780, eBioScience) and Ly6G (Pb, BioLegend) or Ly6G (Pb ...
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bioRxiv - Immunology 2022Quote: ... CD4+ T-cells were cultured alone or co-cultured with either of the B-cell populations at a ratio of 3:1 for 60 h at 37°C and 5% CO2 on anti-CD3 (2 μg/mL) (ULTRA-LEAF Biolegend) coated flat bottomed 384 CellCarrier Ultra microplates (PerkinElmer ...
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bioRxiv - Immunology 2022Quote: ... Single cell suspensions were resuspended in PBS with 2% Newborn Calf Serum and stained with TruStain FcX PLUS (S17011E, BioLegend), APC-labeled Ly6G (1A8 ...
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bioRxiv - Immunology 2022Quote: ... resuspended in 50 μl FACS-buffer containing the respective fluorochrome-coupled Abs (anti-TACI/CD267 PE, clone eBio8F10-3, eBioscience, anti CD138 PECy7, clone 281-2, Biolegend, anti CD19 BV510 ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were then diluted with 50 μL PBS + 2% FBS and FITC anti-CD45 (BioLegend, 304005; 1:1,000 final dilution) and incubated for 20 min on ice in the dark ...
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bioRxiv - Immunology 2023Quote: ... Cells were sorted and purified cells were stained on ice for 30 min in PBS containing 1 % (w/v) BSA with hashtag antibodies (TotalSeq-B0301/B0302/B0303/B0304B0305 anti-mouse Hashtag 1/2/3/4/5, respectively, Biolegend). Cells were washed ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Cells were centrifuged at 500× g at 4 °C and resuspended in 1/3 strength artificial seawater containing 2 μg/mL calcein AM (Enzo) and 100 nM sytox blue (BioLegend) to monitor viability ...
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bioRxiv - Immunology 2023Quote: ... 0.5-1×106 bone marrow cells were stained with the following surface antibodies (2 panels) against: CD14 (Biolegend, M5E2, AF700), HLA-DR (Biolegend ...
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bioRxiv - Cancer Biology 2023Quote: ... 2×106 bone marrow cells were cultured at 37°C in 10ml bmDC culture medium (RPMI 1640 medium with 10% FBS, penicillin/streptomycin and 2-Mercaptoethanol) with 40ng/mL GM-CSF (BioLegend). At day 3 ...
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bioRxiv - Immunology 2023Quote: ... The cells were resuspended in 1 ml R10 and 1-2 million cells were plated in 1ml R10 with 1 μl Brefeldin A (BioLegend) for 4 hours ...
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bioRxiv - Immunology 2024Quote: ... and APC anti-ligand (B7-H6: R&D, clone 875001; CD48: Biolegend, clone; BJ40; Nectin-2: Biolegend, clone TX31; LFA-3: Biolegend, cloneTS2/9 ...
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bioRxiv - Immunology 2024Quote: ... samples were stained on ice for 45 minutes in PBS with 2% Newborn Calf Serum and True-Stain Monocyte Blocker (BioLegend) using a combination of the following antibodies ...
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bioRxiv - Immunology 2024Quote: ... samples were incubated overnight at 4°C with combinations of the following antibodies: PE-Cy7-labeled CD63 (NVG-2, BioLegend), AF532-labeled iNOS (CXNFT ...
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bioRxiv - Genomics 2024Quote: ... A Zombie Violet viability staining was performed by resuspending the cells in 1 mL PBS and adding 2 µl of Zombie Violet (1:1000 dilution, Biolegend). The cells were then kept for 10 minutes in the dark ...
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bioRxiv - Immunology 2024Quote: ... IL-2 (anti-human IL-2, PE-Cy7, clone MQ1-17H12), and CD154 (anti-human CD154, APC, clone 24-31) all from Biolegend in Perm wash buffer (PBS + 0.02% NaN3 + 0.5% bovine serum albumin (BSA ...
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bioRxiv - Neuroscience 2024Quote: ... The cell pellets were resuspended in 100 µL of staining mix (10% FBS in FACS buffer) and 2 µL of allophycocyanin (APC) anti-HA.11 [clone 16B12] (BioLegend). This amount of antibody was empirically tested to yield the highest stain index ...
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bioRxiv - Microbiology 2024Quote: ... in the presence of 10 ng/mL recombinant human IL-2 and 10 ng/mL of recombinant human IL-15 (BioLegend) for 3 days ...
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bioRxiv - Immunology 2024Quote: ... Alexa Fluor 594 conjugated anti-hCD19 (mouse IgG1, R & D Systems) and Alexa Fluor 647 conjugated anti-human CD340 (erbB2/HER-2, Biolegend) antibodies were used for CEACAM5 ...
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bioRxiv - Immunology 2024Quote: ... 4×106 cells from spleen were plated in a 96-well plate and incubated with SIINFEKL peptide and anti-CD107a for 2 hours at 37°C before Brefeldin A (5.0 mg/mL: Biolegend, 420601) and Monensin protein transport inhibitors (4 pL/6E6 cells ...
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bioRxiv - Immunology 2024Quote: ... Specific IgG and IgM antibody levels were measured in culture supernatants by ELISA using LEGEND MAXTM SARS-CoV-2 Spike S1 Human IgG ELISA kit (BioLegend) and LEGEND MAXTM SARS-CoV-2 Spike S1 Human IgM ELISA kit (BioLegend) ...
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bioRxiv - Immunology 2024Quote: ... Autologous CD8+ T cells were isolated from PBMCs using EasySep human CD8+ T cell isolation kit (TEMCELL, Cat# 17953) and stained with 2 μM CFSE (BioLegend) in PBS at r.t ...
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bioRxiv - Immunology 2024Quote: ... and fluorochrome-conjugated anti-CD86 and anti-CD32 antibodies in FACS buffer (1%BSA and 2 mM EDTA in PBS) supplemented with True Stain Monocyte Blocker solution (Biolegend) to prevent aspecific binding of fluorochrome-conjugated antibodies to myeloid cells ...
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bioRxiv - Immunology 2024Quote: Cells were collected and washed in FACS buffer (phosphate-buffered saline supplemented with 2% fetal bovine serum) and subsequently stained with the following antibodies purchased from BioLegend ...
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bioRxiv - Immunology 2024Quote: PBMCs (2×107) of HFRS patients were stained with PE-Cy7-labeled anti-human CD3 mAb (Clone 17A2, BioLegend, USA), APC-Cy7-labeled anti-human CD56 mAb (Clone 5.1H11 ...
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bioRxiv - Neuroscience 2024Quote: ... anti-IgD Pacific Blue (Clone IA6-2, 348224), anti-IgM BV570 (Clone MMH-88, 314517) and Zombie NIR fixable viability dye (423106, Biolegend). Cells were then washed in PBS (D8537 ...
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bioRxiv - Cancer Biology 2024Quote: ... Surface staining of the Macrophage panel consisted of the following primary antibodies diluted in FACS buffer (2% FBS in DPBS) with anti-mouse CD45-Brilliant Violet 650 (1:200, 103151, Biolegend), anti-mouse F4/80-Brilliant Violet 785 (1:200 ...
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bioRxiv - Immunology 2024Quote: ... Sections were saturated with BSA 2% PBS and then incubated overnight with an APC-conjugated anti-CD45 antibody (clone 30F11, Biolegend) at 4°C ...
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bioRxiv - Microbiology 2024Quote: ... LAMP-1 was stained as described previously for LAMP-2 but using a rat-IgG anti-mouse LAMP-1 and anti-rat-IgG-AlexaFluor647 (Biolegend) [56] ...
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bioRxiv - Immunology 2024Quote: Surface staining of lung single cell suspensions was performed in PBS with 2% Newborn Calf Serum on ice for 30 minutes with the following antibodies: TruStain FcX PLUS (S17011E, BioLegend), APC-labeled Ly6G (1A8 ...
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bioRxiv - Molecular Biology 2024Quote: ... The cells were washed with PBS containing 2 mM EDTA and stained with antibodies against CD59 (1:100, anti-human CD59 PE, Biolegend) and C3c (1:25 ...
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bioRxiv - Cell Biology 2024Quote: ... and the supernatant was used for ELISA analysis of IL-2 and latent TGF-beta secretion levels by the kits from Biolegend according to manufacturer’s instructions.
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bioRxiv - Immunology 2020Quote: ... cells were re-stimulated with plate-bound CD3 and soluble CD28 antibodies with the presence of 20 ng/μl IL-2 (Biolegend #589106) for 3 days before infection with HIV-1 reporter viruses ...
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bioRxiv - Immunology 2022Quote: ... and SCT Dd or SCT Dd-CD4 cell surface expression was regularly assessed by flow cytometry (clone 34-5-85 and 34-2-12, Abcam and Biolegend, respectively). Transfectants were FACS-sorted (FACSDiva and FACSARIAIIIU cell sorter ...
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bioRxiv - Immunology 2020Quote: ... Cells were then incubated for 16 hours at 37°C in the presence of 2 uM monensin (Biolegend; San Diego, CA), 5 μg/mL Brefeldin A (Biolegend ...
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bioRxiv - Immunology 2021Quote: ... permeabilized with saponin 0.2% for 20 min and stained with PE-Cy7-anti-IFN-γ mAb and/or PE-anti-IL-10 mAbs (Biolegend). For TF and GzB/PRF staining ...
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bioRxiv - Immunology 2022Quote: ... 0.0032 μg SARS-CoV-2 WT biotinylated RBD protein (His & AVI Tag) conjugated with Brilliant Violet 605 Streptavidin and TotalSeq-C0973 Streptavidin (BioLegend, 405275) and TotalSeq-C0974 Streptavidin(BioLegend ...
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bioRxiv - Neuroscience 2022Quote: ... 2 mice were used whose cells were labelled with Hashtags oligos (HTO) using the Cell Hashing protocol from Biolegend (TotalSeq-A). All the sorted cells were pooled on the same tube and processed in the Chromium Next GEM Chip G using Chromium Next GEM Single Cell 3’ v3.1.
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bioRxiv - Immunology 2022Quote: ... 0.0032 μg SARS-CoV-2 biotinylated RBD protein (His & AVI Tag) (SinoBiological, 40592-V27H-B) conjugated with APC-streptavidin (BioLegend, 405207), and 5 μL 7-AAD (Invitrogen ...
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bioRxiv - Immunology 2022Quote: ... for 30min at 4 °C and intracellular markers (BV421-labeled anti-mouse IFN-γ, Cat# 505830, BioLegend; FITC-labeled anti-mouse IL-2, Cat# 503806, BioLegend; BV711-labeled anti-mouse TNF-α ...
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bioRxiv - Cancer Biology 2022Quote: ... 0.01% 55 mM 2-mercaptoethanol) supplemented with rmGM-CSF (50 ng/mL) and rmFlt3-L (200ng/mL) (Biolegend, San Diego CA). The culture medium was changed on day 9 of culture ...
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bioRxiv - Immunology 2020Quote: ... were added to the tumor cells and incubated for 2 hours with CD20-TCB (200 ng/ml) and +/- αLFA1 antibody (Clone: TS 1/18, 10 μg/ml, BioLegend, 302116). Cells were then gently washed ...
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bioRxiv - Immunology 2020Quote: Supernatants of stimulated T cells were harvested on day 6 of culture and analyzed using the LEGENDplex™ human Thelper 1/2/9/17 multiplex kit (BioLegend). Beads were acquired on a BD Canto II (BD Bioscience ...