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Citations for BioLegend :
801 - 850 of 1006 citations for Recombinant Rat Crp His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... Cells were suspended in PBS + 2% HI-FBS + 2mM EDTA in the presence of Fc receptor blocking antibody (BioLegend, 101302, 1:400) and stained with antibodies at a 1:400 dilution against the following mouse markers ...
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bioRxiv - Cancer Biology 2022Quote: ... or TFH mimicking signals (including 0.02 μg/mL recombinant human IL-4, Peprotech; 0.05 μg/ml recombinant human IL-21, Peprotech, and 5 μg/mL anti-human CD40, Biolegend). Complete RPMI medium contained RPMI 1640 (Gibco ...
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bioRxiv - Immunology 2022Quote: ... neutrophils were incubated with autologous activated platelets (1:10 ratio) (65) for 2.5h and human recombinant CCL28 (50 nM) (BioLegend), the CCR3 antagonist SB328437 (10 mM ...
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bioRxiv - Pathology 2022Quote: ... or B cell supernatant pre-treated with a recombinant antibody to deplete IL-10 (IgG2b, clone JES5-16E3, Biolegend). As controls ...
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bioRxiv - Genetics 2022Quote: ... two-week C57BL/6 c-Kit+ bone marrow cells were cultured in media with recombinant mouse interferon alpha (Biolegend) as indicated with media changes every 2-3 days ...
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bioRxiv - Cancer Biology 2022Quote: Cells cultured in 2D were seeded at 5 × 105 cells/well in 6-well plates and incubated with recombinant human interferon gamma (IFNγ) (0.5 ng/mL) (Biolegend) and/or recombinant tumour necrosis factor alpha (TNFα ...
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bioRxiv - Immunology 2023Quote: ... Human Th17 differentiation assays were performed similarly except the concentrations of recombinant human IL-6 (30 ng/ml, BioLegend), TGF- β1 (2.25ng/mL) ...
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bioRxiv - Microbiology 2023Quote: ... MBL) or H-2q-restricted LCMV NP118 peptide (MBL) and 50 U/mL of recombinant mouse IL-2 (BioLegend) for 1 h ...
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bioRxiv - Immunology 2023Quote: ... NOD female mice were injected intraperitoneally (i.p.) with recombinant mouse IL-33 (0.5-1ug/mice/day, in 100uL of PBS; BioLegend: 580508) or vehicle only (PBS ...
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bioRxiv - Immunology 2023Quote: ... Enriched CellTrace Violet-labeled OT-I CD8+ T cells were resuspended at 20,000 cells/100uL in complete RPMI + 10ng/mL of recombinant mouse IL-2 (Biolegend). As positive control ...
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bioRxiv - Immunology 2023Quote: ... CD73- CD4+ CD25- Foxp3- newly generated CD4SP thymocytes were sorted from Foxp3eGFP-Cre-ERT2 reporter mice and reconstituted at a concentration of 20,000 cells/100uL in complete RPMI + 100ng/mL recombinant mouse IL-7 (Biolegend). CD4SP thymocytes and DC subsets were co-cultured at a 2:1 ratio in round-bottom 96-well plates for 4 days and then analyzed by flow cytometry on day 5 to evaluate the percentage of Tregs generated in vitro.
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bioRxiv - Immunology 2024Quote: ... which is RPMI-1640-based and supplemented with 5 μg/mL recombinant mouse IL-3 (#575506 or #575508, BioLegend) as described in previous studies (6 ...
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bioRxiv - Biochemistry 2020Quote: ... all cell suspensions were Fc blocked with rat anti-mouse CD16/32 (clone 93, BioLegend, USA) and then stained with 10 μg/mL AlexaFluor647 Armenian hamster anti-mouse CD3ε (clone 145-2C11 ...
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bioRxiv - Microbiology 2021Quote: ... rat anti-mouse IL-17-PB (clone TC11-18H10.1, 1:200, cat#506918, BioLegend California, USA), rat anti-mouse TNF-PErCPCy5.5 (clone MP6-XT22 ...
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bioRxiv - Cancer Biology 2021Quote: ... Control mice were injected intraperitoneally with 200 μg of isotype control (Ultra-LEAF rat IgG2b, BioLegend). Mice were injected once with GR1-depleting antibody or isotype control on day 12 post C26 cancer cell subcutaneous injection.
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bioRxiv - Immunology 2022Quote: Deparaffinized lung sections were stained with a purified rat anti-mouse Ly6G antibody (clone 1A8, BioLegend) according to standard immunohistochemical procedures ...
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bioRxiv - Immunology 2022Quote: ... enriched NK cells were stained with surface antibody and biotin-rat-anti mouse NKp46 (29A1.4, Biolegend) as described above ...
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bioRxiv - Immunology 2020Quote: ... 116212) and T cell (PE rat anti-mouse CD4, Biolegend, 557308; FITC anti-mouse CD8a, Biolegend, 100706 ...
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bioRxiv - Cancer Biology 2021Quote: ... followed by overnight staining at 4°C with rat anti-mouse CD45 primary antibody (103102, Biolegend) diluted 1:500 in 1x D-PBS ...
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bioRxiv - Immunology 2022Quote: ... followed by rat anti-human Fc antibody conjugated to APC (Cat: 410712, BioLegend; 1:100 dilution) for 15 min on ice in the dark for secondary detection ...
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bioRxiv - Cancer Biology 2020Quote: ... and then stained with rat a mAb to EEA-1 (1G11; eBioscience) or TLR3 (11F8 ; Biolegend), followed by Alexa Flor 488-conjugated donkey anti-mouse IgG or Alexa Flor 546-conjugated goat anti-rat IgG (Invitrogen) ...
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bioRxiv - Cell Biology 2020Quote: ... BMDCs were stained using rat anti-mouse CD45-AF647 (clone 30-F11, 1:250, Biolegend, 103123), and Factin and cell nuclei were visualized using respectively phalloidin-TRITC (P1951-1MG ...
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bioRxiv - Immunology 2021Quote: ... anti-rat CD2 (OX-34) and biotin anti-phycoerythrin (PE)(PE001) antibodies were purchased from BioLegend. Rat IgG2b κ Isotype Control (eB149/10H5 ...
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bioRxiv - Immunology 2019Quote: ... Frozen spleen sections were stained with F4/80 (PE-rat anti-mouse #123019 (Clone BM8, BioLegend) or CD206-(Alexa Fluor-647 rat anti-mouse #321116 (Clone 15-2 ...
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bioRxiv - Developmental Biology 2020Quote: ... Guinea pig anti-Cytokeratin 8+18 antibody and FITC rat anti-mouse/human CD45R/B220 (BioLegend) were used for fluorescence immuno-histochemistry ...
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bioRxiv - Neuroscience 2021Quote: ... sections were co-stained with a primary monoclonal rat antibody against CD31 (1:500, BioLegend (102502)) ...
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bioRxiv - Immunology 2020Quote: ... which included AF647-conjugated rat anti-mouse IgD (clone 11-26 c.2a, Biolegend; 1:200), FITC-conjugated rat anti-mouse Ki67 (clone SolA15 ...
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bioRxiv - Microbiology 2022Quote: ... GC B cells stained using rat anti-GL7 antibody (FITC; BioLegend, catalog no. 144604, 1:250), Tfh cells stained using anti-CD4 antibody (BioLegend ...
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bioRxiv - Immunology 2022Quote: ... slices were incubated with 20 μg/ml anti-CD45 (Pac-Blue-αCD45 Rat IgG2a, κ, BioLegend). Slices were washed 3x with 1X PBS for 10 min per wash.
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bioRxiv - Neuroscience 2023Quote: ... The Cells were then sorted using ICAM2 (rat anti-mouse CD102, Clone 3C4, Catalog #105602, BioLegend) and purified (Dynabeads™ Protein G 10003D).
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bioRxiv - Immunology 2023Quote: ... rat anti-mouse Lineage cocktail (conjugated with Pacific blue, cat#133310, Biolegend, Inc, San Diego, CA) consisting of rat anti-mouse CD3 (clone ...
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bioRxiv - Cell Biology 2023Quote: ... Secondary antibodies were allophycocyanin (APC)-conjugated monoclonal rat anti-mouse IgG1 RMG1–1 (Biolegend 1:50) or goat anti mouse IgG poly4053 (Biolegend 1:50) ...
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bioRxiv - Immunology 2023Quote: ... 100 ug/mouse or purified rat IgG2a,k isotype ctrl antibody (clone RTK2758, BioLegend Cat#400502) 100 ug/mouse ...
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bioRxiv - Immunology 2024Quote: ... KO1, KO2) and treatment conditions (no IFN, IFN-β, IFN-λ) were incubated with separate oligonucleotide-tagged antibodies (HTO) for multiplexing (Biolegend TotalSeq, A0301 - A0309). Groups were counted and pooled in equal abundance ...
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bioRxiv - Immunology 2022Quote: ... This is followed by secondary staining for 30 min at 4°C with APC-conjugated mouse anti-His antibody (BioLegend, #362605, 1:50) or isotype control ...
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bioRxiv - Immunology 2021Quote: ... in culture medium supplemented with 0.5 µg/mL soluble anti-mouse CD28 (eBioscience) and 10 ng/mL (∼50 U/mL) recombinant murine Interleukin-2 (IL-2, BioLegend), and activated for up to 72h ...
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bioRxiv - Microbiology 2021Quote: ... 1 U/ml penicillin/streptomycin) with 3 ng/ml recombinant mouse GM-CSF (rGM-CSF; R&D Systems or BioLegend)) at a concentration of 2.5 x 105 cells/ml in 150 x 15 mm petri plates at 37°C with 5% CO2 ...
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bioRxiv - Immunology 2022Quote: ... Frozen PBMC of post-CA patients were thawed and incubated in media (RPMI1640 + 2% FBS) with recombinant human Nectin-2 (Biolegend) or isotype control (ENZO ...
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bioRxiv - Immunology 2021Quote: ... The antigenicity and the immunogenicity of the purified recombinant spike protein was validated (Supplementary Fig 1) against verified available recombinant spike protein (BEI Resources, NR52397) and anti-SARS-CoV-2 spike antibody clone A20085C (BioLegend).
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bioRxiv - Immunology 2022Quote: Bone marrow cells from femurs and tibias were cultured in presence of 20 ng/ml mouse recombinant GM-CSF (Biolegend) for 8 days ...
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bioRxiv - Neuroscience 2022Quote: ... neuronal activity was recorded for 15– 30 min daily before and after treatment with recombinant CXCL11 (200 nM, BioLegend #573606) using the Maestro Pro MEA System (Axion BioSystems) ...
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bioRxiv - Immunology 2022Quote: ... 5×104 B cells were first incubated with 5 µg/ml anti-IgM complexes and 1 µg/ml recombinant Human CD40L (Biolegend) for 30 mins and then 1×104 Tfr cells were added ...
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bioRxiv - Immunology 2023Quote: ... with recombinant IL-15/IL-15RA complex (100 ng/ml; Thermo Fischer, Waltham, MA) or recombinant mouse IL-6 (50 ng/ml, BioLegend), TGF- β1 (1ng/mL) ...
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bioRxiv - Physiology 2023Quote: ... zoledronic acid (Novartis Pharmaceuticals) and/or 50 μg/kg (i.p.) recombinant human granulocyte-colony stimulating factor (G-CSF; carrier-free, BioLegend #578604) for three days ...
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bioRxiv - Immunology 2023Quote: ... Cells were incubated at 37°C and 5% CO2 for one hour to settle and then stimulated with 20 ng/mL recombinant IFNg (BioLegend), 100 ng/mL LPS-EB Ultrapure (Invivogen ...
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bioRxiv - Cancer Biology 2023Quote: ... T cells were incubated for 48h with OVAp (10 ng/ml) and mouse recombinant IL2 (50 U/ml) (Cat# 575402, Biolegend), was added for a period of five additional days ...
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bioRxiv - Cancer Biology 2024Quote: ... T cells were activated with αCD3/αCD28 Dynabeads as specified by manufacturer’s instructions in the presence of 100 IU/mL recombinant mouse (rm) IL-2 and 10 ng/mL rmIL-7 (BioLegend) and cultured in RPMI1640 supplemented with 10% FBS ...
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bioRxiv - Immunology 2023Quote: ... 200 μl for 96-well plates) supplemented with 1 μg/ml of recombinant human B cell-activating factor (BAFF, BioLegend). 1 µg/ml rHA from H1N1 (PR8 ...
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bioRxiv - Immunology 2024Quote: ... were cultured for 3 days in 96-well U bottom plates coated with 2 μg/mL anti-CD3 (eBioscience) and 1 μg/mL anti-CD28 (eBioscience) supplemented with 10ng/mL of recombinant mIL-2 (Biolegend) to allow Treg survival ...
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bioRxiv - Cancer Biology 2019Quote: ... was from BioXCell (Lebanon, NH), and rat anti-murine CD3 (Cat no 100331, LEAF purified) from Biolegend (San Diego, CA).