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Citations for BioLegend :
801 - 850 of 3486 citations for Recombinant Human PDCD1 Protein Fc Avi His tagged Biotinylated since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... human CD8 (BioLegend #344712), human CD45 (BioLegend #304014) ...
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bioRxiv - Microbiology 2023Quote: ... Human TruStain FcX (BioLegend) was added at a 1:10 dilution ...
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bioRxiv - Microbiology 2023Quote: ... Human TruStain FcX (BioLegend) was added at a 1:10 dilution ...
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bioRxiv - Immunology 2024Quote: ... Human TruStain FcX (BioLegend) was added at 1:20 to block Fc receptors for 10 minutes ...
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bioRxiv - Immunology 2024Quote: ... human TGFβ1 (580706; BioLegend), human IL-12 (219-IL;R&D Sys-tems) ...
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bioRxiv - Immunology 2020Quote: ... The remaining cells were then labelled with biotinylated anti-CD45.2 Ab (Clone 104, Biolegend, #109804) at a 1:100 dilution for 30 min ...
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bioRxiv - Immunology 2019Quote: ... OVA and HEL were biotinylated in-house and tetramerised with streptavidin-PE (Biolegend – CA, USA), streptavidin-APC (Biolegend – CA ...
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bioRxiv - Immunology 2021Quote: ... The wells were incubated with biotinylated anti-mouse IFN-γ (clone R4-6A2, Biolegend, USA) for 16 hours at 4° C ...
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bioRxiv - Microbiology 2019Quote: ... The biotinylated secondary antibody was stained subsequently with streptavidin-PE-Cy7 (Biolegend, 1:200 dilution). After washing the cells ...
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bioRxiv - Cancer Biology 2022Quote: ... we coated the cells with 3.5 μg/ml biotinylated anti-CD81 (BioLegend, clone TAPA-1) antibody at the 37 °C for 30 min ...
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bioRxiv - Genomics 2023Quote: ... Control wells were created in the same manner by immobilising commercially biotinylated Mouse IgG2a (Biolegend) alone at a concentration of 0.5ug/ul ...
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bioRxiv - Immunology 2023Quote: MHC-I tetramers were generated using the biotinylated Flex-T™ monomer UVX platform (Biolegend) for HLA-A*02:01 and HLA-A*03:01 according to manufacturer’s protocol with the modifications detailed below ...
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bioRxiv - Immunology 2023Quote: ... and stained for 5 min at 4°C with biotinylated anti-CD3 antibody (17A2; BioLegend) to exclude CD3-positive T cells in the cell suspension using anti-biotin microbeads (Miltenyi Biotec ...
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bioRxiv - Neuroscience 2020Quote: 250 μg protein from each human brain sample lysate was used for immunoprecipitation (IP) with a combination of Tau12 (Biolegend, cat. no. 806501), Tau 5 (Abcam ...
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bioRxiv - Synthetic Biology 2021Quote: ... Anti-bodies used in this study included a monoclonal α-his-antibody (BioLegend, 652502) and polyclonal α-CsgA-antibody and α-TasA-antibody.
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bioRxiv - Bioengineering 2022Quote: ... Confirmation of successful bead conjugation was determined by staining with anti-His Tag (Biolegend) and flow cytometry analysis ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... The Western blot membranes were stained with HRP anti-His tag antibody (Biolegend, #652504) diluted 1:2,000 in 0.5% (w/v ...
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bioRxiv - Cell Biology 2021Quote: ... and an anti-FLAG AF549 conjugate (for cells expressing FLAG-tagged M; 1:1000, Biolegend, 637314) for 20 minutes at room temperature ...
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bioRxiv - Bioengineering 2021Quote: ... containing 1/100 diluted anti-human CD3 or anti-human TCRα/β (Biolegend), both targeting the TCR complex ...
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bioRxiv - Immunology 2022Quote: ... anti-human CD57-PB (BioLegend, Cat#322316], anti-human CD16-V500 [BD Biosciences, Cat#561314], anti-human CD69-PE-Cy7 [BioLegend, Cat#310190]). 5×105 to 1×106 cells were re-suspended in residual buffer and antibody panels added and incubated on ice for 20 to 30 minutes ...
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bioRxiv - Immunology 2019Quote: ... Cells were washed in FACS buffer and incubated with Fc block (Biolegend, UK) before staining with the relevant antibodies/streptavidin conjugates ...
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bioRxiv - Immunology 2021Quote: ... Single cell suspensions were incubated in Fc-receptor blocking antibody (Clone 93, Biolegend) during cell surface antigen staining ...
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bioRxiv - Immunology 2022Quote: ... MEL624 was pre-treated with hIgG1-Fc (10ug/ml) (Biolegend, San Diego, CA) or Siglec-5-Fc chimeric protein (10ug/ml ...
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bioRxiv - Cancer Biology 2022Quote: ... resuspended in PBS/1% FCS and stained with anti-B220 (RA3-6B2, Biolegend), anti-Igµ (II/41 ...
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bioRxiv - Immunology 2020Quote: ... After blocking of Fc receptors (anti-mouse CD16/CD32, TruStain fcX from BioLegend), cells were labelled with biotin-conjugated antibody cocktail containing anti-CD45.1 and anti-Lin (anti-CD3 ...
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bioRxiv - Immunology 2021Quote: ... and incubated with Fc block (1:100; Biolegend TruStain fcX, cat no. 101320) and fluorophore-conjugated antibodies at the appropriate concentrations (Table 3 ...
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bioRxiv - Immunology 2021Quote: ... cells were stained separately with Fc blocker (concentration 1:20) (TruStain FcX, Biolegend), rested for 15 minutes ...
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bioRxiv - Immunology 2020Quote: ... cell suspensions were Fc-blocked using anti-CD16/32 mAb (BioLegend, clone 93) combined to murine serum 4% (Thermo Fischer) ...
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bioRxiv - Immunology 2022Quote: ... Fc receptors were blocked with anti-mouse CD16/32 (TruStain FcX™, Biolegend). Cells were then incubated with fluorochrome-conjugated antibodies for 20 min at 4°C and washed twice with PBS ...
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bioRxiv - Microbiology 2022Quote: ... Single cell suspensions of lung digest were preincubated with Fc block antibody (Biolegend) in PBS + 2% heat-inactivated FBS + 1 mM EDTA for 20 min at 4°C ...
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bioRxiv - Microbiology 2022Quote: ... cells were washed and stained with Fc block (Clone 93; Cat: 101320; BioLegend), CD45 (BUV395 ...
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bioRxiv - Immunology 2023Quote: ... All samples were then incubated with Fc block (5µg/mL CD16/CD32 (Biolegend) and 0.1% mouse serum (Sigma ...
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bioRxiv - Bioengineering 2022Quote: ... Cells were preincubated in FC Blocking solution (TruStain FcX™ PLUS, Biolegend, 156603) and Super Bright Staining Buffer (Invitrogen ...
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bioRxiv - Genomics 2022Quote: ... After the Fc blocking with 10 μl of FcX (BioLegend, cat# 422301/ 101319) for 10 minutes on ice ...
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bioRxiv - Immunology 2023Quote: ... Fc receptors were blocked with anti-mouse CD16/32 antibodies (TruStain FcX, BioLegend) in FACS buffer for 10 minutes at 4°C ...
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bioRxiv - Microbiology 2023Quote: ... and incubated with Fc block (purified anti-mouse CD16/CD32 antibody, Biolegend 101302) at 1:200 in Cell Staining Buffer for 10 min at 4°C ...
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bioRxiv - Genomics 2023Quote: ... Cells were then resuspended in 100 mL/well of FC-block (Biolegend, 422302) (1:100 in Perm buffer ...
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bioRxiv - Cell Biology 2024Quote: ... Fc receptors were blocked with 10 µL TruStain FCX receptor block (BioLegend, 422301) for 10 minutes at room temperature ...
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bioRxiv - Cancer Biology 2021Quote: ... then treated with recombinant mouse IFNβ1 (cat. # 581302, BioLegend, San Diego, CA) at 28 ...
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bioRxiv - Immunology 2021Quote: ... injection of 8 μg/mouse of recombinant interferon-α from Biolegend (752806) was administered ...
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bioRxiv - Immunology 2019Quote: ... 10% FBS and 20 ng/ml of murine recombinant M-CSF (Biolegend) at 37°C in a humidified incubator for 7–8 days ...
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bioRxiv - Microbiology 2022Quote: ... plated in complete RPMI with 50 ng/mL recombinant M-CSF (Biolegend) and cultured for 6 days with medium change every 3 days.
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bioRxiv - Immunology 2021Quote: ... cultures were additionally supplemented with 5ng/mL recombinant mouse IL-7 (Biolegend). Scid.adh.2c2 cells were cultured in IMDM supplemented with 10% FBS (Hyclone) ...
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bioRxiv - Cancer Biology 2022Quote: ... recombinant mouse Oncostatin M and IL-6 (Biolegend, Cat #: 762802 & 575702 respectively).
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bioRxiv - Physiology 2023Quote: ... with or without EPO (35U/well) (recombinant mouse EPO carrier-free, Biolegend). The ratio of splenocytes to RBCs was 1:1 ...
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bioRxiv - Microbiology 2023Quote: ... plated in complete RPMI with 50 ng/mL recombinant M-CSF (Biolegend) and cultured for 6 days with medium change every 3 days.
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bioRxiv - Cancer Biology 2024Quote: ... in the presence of 10 ng/mL recombinant MIF (Biolegend, cat. 599404) or MDK (Biolegend ...
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bioRxiv - Immunology 2020Quote: BM neutrophils were isolated using MACS-column purification (Millentyi Biotec) with biotinylated mouse Ly6G antibodies (BioLegend) and streptavidin magnetic beads (Millentyi Biotec ...
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bioRxiv - Cell Biology 2021Quote: ... CD4+CD25- T cells (Tconv) were purified by adding a biotinylated anti-CD25 antibody (PC61; Biolegend) to the CD4+ T cell negative isolation kit to deplete Treg cells ...
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bioRxiv - Cancer Biology 2021Quote: ... then cells were stained with antibodies for 30 mins on ice: biotinylated anti-PDPN (BioLegend 127404), biotinylated anti-PDGFRα (anti-CD140a ...