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Citations for BioLegend :
801 - 850 of 7619 citations for 6 Iodo 2 methyl 4H 3 1 benzoxazin 4 one since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... 0.5-1×106 bone marrow cells were stained with the following surface antibodies (2 panels) against: CD14 (Biolegend, M5E2, AF700), HLA-DR (Biolegend ...
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bioRxiv - Cell Biology 2024Quote: ... 2 mM EDTA in PBS) and incubated with Fc-blocking reagent (1:200, TruStain FcX, anti-mouse CD16/32, BioLegend). After washing ...
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bioRxiv - Immunology 2024Quote: ... cells were washed with FACS staining buffer (PBS containing 2% fetal bovine serum [FBS] and 1 mM EDTA) and incubated with an Fc receptor blocker (TrueStain fcX; BioLegend) for 5 minutes at 4℃ ...
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bioRxiv - Immunology 2024Quote: ... were cultured for 3 days in 96-well U bottom plates coated with 2 μg/mL anti-CD3 (eBioscience) and 1 μg/mL anti-CD28 (eBioscience) supplemented with 10ng/mL of recombinant mIL-2 (Biolegend) to allow Treg survival ...
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bioRxiv - Developmental Biology 2024Quote: ... and the resulting cDNA was amplified using as PCR oligos the partial TruSeq read 1 adaptor (provided in the 10x kit) and an additive primer (a partial TruSeq read 2, sequence provided by Biolegend and synthesized by Integrated DNA Technologies) ...
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bioRxiv - Immunology 2024Quote: ... plates were coated per well with 100 µL of anti-IgE capture antibody (2 µg/mL in PBS, clone RME-1, BioLegend) overnight at 4⁰ C ...
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bioRxiv - Immunology 2022Quote: ... 3 μL PE/Cyanine7 anti-human IgM antibody (BioLegend, 314532), 0.0052 μg biotinylated Ovalbumin (SinoBiological ...
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bioRxiv - Immunology 2021Quote: ... FITC anti-human CD223 (LAG-3) antibody (clone 11C3C65, BioLegend), BV510 anti-human CD314 (NKG2D ...
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bioRxiv - Molecular Biology 2020Quote: ... anti-Rpb3 (Neoclone W0012, 3 μL; BioLegend 665004, 1.5 μL), anti-Flag (Sigma-Aldrich F3165 ...
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bioRxiv - Immunology 2020Quote: ... The following antibodies were used: anti-LAG-3 (11C3C65, BioLegend), anti-PD-1 (EH12.1 ...
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bioRxiv - Immunology 2020Quote: ... FITC anti-human CD223 (LAG-3) antibody (clone 11C3C65, BioLegend), BV510 anti-human CD314 (NKG2D ...
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bioRxiv - Immunology 2020Quote: ... CD127 AF488 (clone A019D5, BioLegend, cat# 351314, dilution 3:100); CD25 PE-Cy7 (clone BC96 ...
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bioRxiv - Immunology 2021Quote: ... washed for 3 times with Washing Buffer (BioLegend Cat#421601) and blocked with Dilution Buffer (0.5M Tris pH 8.0 ...
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bioRxiv - Cancer Biology 2021Quote: ... cells were incubated with PE-Galectin-3 (Biolegend cat# 126706) or matched isotype control for 15 mins and analyzed on BD Accuri C6 cytometer (BD Biosciences ...
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bioRxiv - Genomics 2023Quote: ... TotalSeq™-B0253 anti-human Hashtag 3 Antibody (394635, Biolegend), TotalSeq™-B0254 anti-human Hashtag 4 Antibody (394637 ...
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bioRxiv - Immunology 2024Quote: ... injection of 3 µg anti-CD45.2-PE (clone 104, Biolegend) antibody 3 min prior to sacrifice ...
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bioRxiv - Immunology 2024Quote: ... and anti-mouse α-CD28 (3 μg/ml, Biolegend, #102116) in RPMI medium supplemented with 10% FBS with or without dFB-CM ...
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bioRxiv - Biochemistry 2020Quote: ... Traditional antibodies based labeling procedure was done by using c-Myc Antibody (9E10, Cat # 626801, BioLegend, USA; incubation 1 h at 4°C) and Anti-Mouse IgG (Fc specific)-FITC antibody produced in goat (Cat # F4143 ...
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bioRxiv - Immunology 2022Quote: ... This is followed by secondary staining for 30 min at 4°C with APC-conjugated mouse anti-His antibody (BioLegend, #362605, 1:50) or isotype control ...
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bioRxiv - Systems Biology 2019Quote: ... We washed the samples again with 0.1% BSA-PBS and then incubated for 10 minutes at 4°C with 1:250 anti-NGFR antibody conjugated directly to PE-Cy7 (Biolegend, 400126, clone ME20.4) in 0.5% BSA-PBS with 2mM EDTA ...
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bioRxiv - Neuroscience 2019Quote: ... The triangularis sterni muscle was dissected and incubated overnight (4°C) in anti-βIII-tubulin antibody conjugated to Alexa488 (BioLegend 801203; 1:200) and Alexa594-conjugated α-bungarotoxin (Invitrogen B13423 ...
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bioRxiv - Immunology 2020Quote: ... and then incubated in the dark for 30 min at 4°C: Myeloid-lymphocyte common lineage panel – TruStain FcX (anti-CD16/32) (clone: 93; dilution: 1:100, BioLegend, San Diego, CA), CD45-Pacific Blue (clone ...
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bioRxiv - Immunology 2022Quote: ... Cells were centrifuged for 5 min at 500 x g and 4 °C and stained with Fc receptor blocker (TruStain fcX™, BioLegend, 1:100) and viability stain (Zombie Aqua™ Fixable Viability Kit ...
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bioRxiv - Cancer Biology 2023Quote: ... and stained overnight at 4°C in blocking solution with the following primary antibodies and dilutions: anti-Nestin (1:150, Biolegend, #656802, clone 10C2), anti-EGFR (1:300 ...
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bioRxiv - Bioengineering 2024Quote: ... Sections were then stained overnight at 4 °C in a humidified chamber with rat monoclonal anti-mouse CD68 (1:400, BioLegend, clone FA-11) and rabbit polyclonal anti-mouse IL-10 (1:25 ...
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bioRxiv - Bioengineering 2024Quote: ... Sections were then stained overnight at 4 °C in a humidified chamber with mouse monoclonal anti-human CD68 (hCD68, 1:400, BioLegend, clone Y1/82A) and rabbit polyclonal anti-mouse IL-10 (mIL-10 ...
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bioRxiv - Neuroscience 2020Quote: ... the neurons were fixed and stained as previously described38 using primary antibodies for β-3-Tubulin (TUJ1; 1:200; BioLegend, San Diego, CA, USA; 801201) and TH (1:300 ...
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bioRxiv - Immunology 2023Quote: ... IL-2 secretion was evaluated with the IL-2 assay ELISA kit (Cat#431801, Biolegend). The absorbance value at 450 nm/570 nm was detected by the microplate reader ...
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bioRxiv - Cell Biology 2022Quote: ... one million cells were first resuspended in 100 μL buffer containing 5 μL Fc Receptor Blocking Solution (Biolegend, #422302) and incubated for 15 minutes ...
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bioRxiv - Immunology 2021Quote: ... The splenocytes were incubated for 6 hr at 37°C in vitro with BrefeldinA (Biolegend) under three conditions ...
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bioRxiv - Neuroscience 2022Quote: Enzyme-linked immunosorbent assays were performed using the mouse IL-6 ELISA-MAX kit (Biolegend), using manufacturer’s instructions.
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bioRxiv - Cancer Biology 2022Quote: NSG mice were administrated intraperitoneally with IL-6 (50 μg /mouse) neutralizing Ab (BioLegend 504512) for two days ...
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bioRxiv - Immunology 2020Quote: ... and then stimulated with 10nM HyIL-6 or 0.1 μg/mL anti-CD3 (Biolegend #300438) and 20 ng/mL IL2 (Novartis #709421) ...
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bioRxiv - Immunology 2021Quote: ... and supernatants were harvested for IL-6 and IL-1β ELISA (Biolegend, San Diego, CA). BMDCs were also utilized in the MLR and in vitro T cell differentiation assays.
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bioRxiv - Immunology 2022Quote: ... and then stained with PE-conjugated anti-mouse IL-6 mAb (clone MP5-20F3, Biolegend). Stained cells were analysed using a BD LSR Fortessa cell analyser (BD Biosciences ...
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bioRxiv - Cancer Biology 2023Quote: ... and PE anti-mouse H-2Kb/H2Db antibody (Clone 28-8-6; Biolegend; Cat #114607) on ice for 20 minutes in the dark ...
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bioRxiv - Immunology 2023Quote: ... macrophages were challenged with CFSE (5-[and 6]-Carboxyfluorescein diacetate succinimidyl ester Kit, BioLegend,423801)-labeled apoptotic cells at a 4:1 (AC/Macrophage ...
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bioRxiv - Immunology 2024Quote: WT C57Bl/6 mice were injected intraperitoneally with 250 ng recombinant mouse IL-33 (Biolegend) in 100 µl PBS for three consecutive days ...
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bioRxiv - Immunology 2019Quote: ... 105 resting NK cells were mixed at 1:1 with 221 cell lines or peptide loaded target cells in presence of 1 μl BV421 anti-CD107a mAb for 2 hr at 37°C (H4A3, Biolegend 328626). NK cells were then stained with mAbs to identify subsets based on the expression of KIR2DL1/S1 (Beckman Coulter ...
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bioRxiv - Immunology 2020Quote: ... were added to the tumor cells and incubated for 2 hours with CD20-TCB (200 ng/ml) and +/- αLFA1 antibody (Clone: TS 1/18, 10 μg/ml, BioLegend, 302116). Cells were then gently washed ...
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bioRxiv - Immunology 2019Quote: ... Antibody coated cells were mixed with resting NK cells in the presence of 1 μl BV421 anti-CD107a mAb for 2 hr at 37°C (H4A3, Biolegend 328626). Cells were then stained with mAbs to KIR2DS4 (Miltenyi Biotec ...
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bioRxiv - Immunology 2021Quote: ... Approximately 2 million cells per experimental condition were stained with 1 μg of TotalSeq™ B0301-B0308 anti-mouse Hashtag antibodies (BioLegend) according to the manufacturer’s instructions and pooled together at equal cell numbers ...
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bioRxiv - Bioengineering 2023Quote: THP-1 cells were incubated with 2 µg/ml of anti-CD44 antibodies (clone: C44Mab-5; Biolegend, San Diego, CA, USA) for 20 minutes under standard cell culture conditions (95% humidity and 5% CO2 at 37°C ...
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bioRxiv - Immunology 2023Quote: ... For the memory B cell EliSpot, PBMC were stimulated with 1 µg/ml R848 (SigmaAldrich, SML0196) and 10 ng/ml recombinant human IL-2 (Biolegend, 791904) for 5 days before harvesting and plating of 50-100.000 cells/well ...
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bioRxiv - Cell Biology 2024Quote: ... and the resulting suspension was plated at 1-2×106 cells/ml in complete medium containing 20% FCS and 20 ng/ml M-CSF (BioLegend, 574804). Cells were incubated for 6 days with a medium replacement at day 3 ...
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bioRxiv - Immunology 2024Quote: ... Cells were then washed with FACS buffer (PBS+ 2% (v/v) FBS with 1mM EDTA) and incubated with a 1:500 dilution of TruStain FCX (Biolegend, 101320) for 5 minutes ...
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bioRxiv - Microbiology 2021Quote: Concentrations of cytokines and chemokines present in the serum from SARS-CoV-2 infected AGMs were quantified using a multiplex bead-based assay (1:4 dilution)-the LEGENDPlex Non-Human Primate Cytokine/Chemokines 13-plex (BioLegend, San Diego, CA USA). Analytes detected by this panel are the following ...
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bioRxiv - Cancer Biology 2022Quote: ... Human tissues were incubated for 25 min at 4 °C with the following primary antibodies: CD24-PE (BioLegend, clone ML5, Cat, #: 311105, 1:100), CD44-FITC (BioLegend ...
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bioRxiv - Cancer Biology 2022Quote: ... Tissue was incubated in primary antibody overnight at 4°C in a moist chamber diluted in blocking buffer with the following dilutions: Anti-Tubulin β3 (BioLegend, clone TUJ1, 1:500); Anti-OMP (Santa Cruz ...
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bioRxiv - Immunology 2022Quote: Antibody staining was done in the dark for 30 minutes at 4°C in 30 μL of FACS buffer with antibodies and anti-CD16/32 (TruStain FcX™ PLUS; BioLegend, #156604; 1:100). Acquisition was done on a BD FACSCanto™ II or BD LSRFortessa (both BD Biosciences) ...