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Citations for BioLegend :
801 - 850 of 7633 citations for 6 BROMO 4 4 DIMETHYL 1H BENZO D 1 3 OXAZINE 2 4H THIONE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2024Quote: ... for 10 min at room temperature and then stained for 30 min at 4°C with the following antibody cocktail: anti-mouse CD45.1 (Biolegend, cat.: 110735), anti-mouse TER-119 (eBiosciences ...
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bioRxiv - Immunology 2024Quote: ... for 4 h and stained for intracellular expression of either IL-4 or FOXP3 after fixation and permeabilization using the Fixation Buffer (BioLegend, # 420801), the Intracellular Staining Perm Wash Buffer (BioLegend ...
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bioRxiv - Immunology 2022Quote: ... All samples were also stained with a unique anti-mouse TotalSeq-A Hashtag antibody (1-6; BioLegend) to allow up to 6 populations to be multiplexed in a single lane on a 10X Genomics Chromium Next GEM Chip96 ...
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bioRxiv - Cell Biology 2022Quote: ... and IL-6 (MP5-20F3, Biolegend) were used where indicated.
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bioRxiv - Microbiology 2021Quote: Anti-IL-6 ELISA (BioLegend 430504) was performed according to manufacturer’s protocols ...
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bioRxiv - Immunology 2020Quote: ... 100ng/ml IL-6 (Biolegend #570802), 30ng/ml IL-1β (Biolegend #479402) ...
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bioRxiv - Immunology 2020Quote: ... BCL-6 APC (IG191E/A8; Biolegend) and Ki67 BUV395 (B56 ...
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bioRxiv - Bioengineering 2022Quote: ... IL-6 (Biolegend, San Diego, CA) at 650 μg/mL ...
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bioRxiv - Immunology 2023Quote: ... 50 ng/mL IL-6 (Biolegend), and 50 ng/mL stem cell factor (Biolegend) ...
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bioRxiv - Immunology 2023Quote: ... rat anti-IL-6 (Biolegend 504501); rabbit anti-COL14A1 (Novus Biologicals NBP2-15940) ...
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bioRxiv - Immunology 2022Quote: ... tissue sections were blocked with 3% BSA and Human TruStain FcX (1:100, BioLegend) in PBST for 1 hour at room temperature ...
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bioRxiv - Immunology 2020Quote: ... Cells were fixed with 3% paraformaldehyde and stained with anti-LFA-1 (BioLegend, 301202) and phalloidin-AF 488 (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2023Quote: ... cells were incubated with 200 µg/mL recombinant Galectin-1 and Galectin-3 (Biolegend). Galectin binding was competitively inhibited by adding 50 mM lactose during the staining procedure.
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bioRxiv - Bioengineering 2022Quote: ... For immune cells detections the tumoroids sections also were incubated overnight at 4°C with anti-B220 (Biolegend 103224, APC-cy7 conjugated) and anti-cd11b (Biolegend 101208 ...
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Single cell analysis of the cellular heterogeneity and interactions in the injured mouse spinal cordbioRxiv - Neuroscience 2020Quote: ... supernatant was aspirated and the cells were incubated in blocking buffer mixture for 5min at 4°C (1uL anti-mouse CD16/32 TruStain Fcx (RRID: AB-1877271 AB-1574973, Biolegend Cat #: 101320) and 99uL FACS buffer (1% BSA + 0.05% sodium azide in HBSS w/o Ca2+/Mg2+)) ...
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bioRxiv - Cell Biology 2021Quote: ... the cells were collected by centrifugation (1500rpm; 5mins; 4°C) and washed twice with Annexin V binding buffer (Biolegend; 1ml per wash). The cell pellets were resuspended in of binding buffer (100μl ...
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bioRxiv - Immunology 2020Quote: ... Unbound antibodies were removed by washing with PBS were added at 5 × 104 cells/well in the presence of 4 μg/mL brefeldin A (Biolegend, Cat # 420601), 5 μg/mL GolgiStop (BD Biosciences ...
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bioRxiv - Immunology 2020Quote: ... Then these PBMCs was incubated with mixed antibodies cocktail at 4 °C for 30 min (the antibodies cocktail including FITC-anti-human CD19 antibody (Biolegend, clone: SJ25C1), BV421-anti-human IgD antibody (Biolegend ...
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bioRxiv - Genetics 2021Quote: ... The cells were then incubated for 20 min at 4°C with the TotalSeq-C antibody mix: TotalSeq - C0048 anti-human CD45 Antibody (Biolegend; Cat# 368545), TotalSeq - C0103 anti-mouse/human CD45R/B220 (Biolegend ...
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bioRxiv - Immunology 2021Quote: ... Cells were washed and stained in PermWash buffer (eBioscience) for 30 min at 4°C with Abs (purchased from BioLegend unless noted) against Bcl2 (BCL/10C4) ...
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bioRxiv - Immunology 2021Quote: ... Cell surface proteins were stained for 20 min at 4 °C with the following antibodies: CD45.1 (BioLegend, 110714, Clone ID: A20, RRID:AB_313503), CD45.2 (BioLegend ...
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bioRxiv - Immunology 2021Quote: 4*106 peripheral blood mononuclear cells (PBMCs) per patient were first incubated with Human TruStain FcX™ blocking reagent (Biolegend, Cat# 422302) for 10 minutes at 4°C ...
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bioRxiv - Immunology 2022Quote: ... Staining for surface markers of T cells was carried out in the dark at 4°C for 15 – 30 minutes using the following antibody panel: BV510-CD45 (clone 30F11; Biolegend, CA, USA), PE-Cy7-TCRβ (clone H57-597 ...
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bioRxiv - Neuroscience 2022Quote: ... Cell suspensions were treated with anti-mouse-CD16/32 to block Fc receptors (BioLegend, 5 μg/mL, 20 min at 4 °C) before washing and transferring to PBS and treating with Zombie NIR (BioLegend ...
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bioRxiv - Immunology 2022Quote: ... Cells were incubated with Fixable Viability Stain 510 for 15 minutes at 4 °C followed by anti-Fc blocking reagent (Biolegend, Cat# 101320) for 10 minutes prior to surface staining ...
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bioRxiv - Immunology 2023Quote: ... in FACS buffer for 30 minutes at 4°C prior to being stained with fluorescently conjugated antibodies to CD3e (Biolegend, clone 17A2), CD44 (Biolegend ...
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bioRxiv - Immunology 2023Quote: ... Sections were stained using a three-colour protocol with 1:100 of each of the following antibodies in IF blocking buffer at 4 °C O/N: Alexa Fluor 488® anti-mouse GL-7 (GL-7; Biolegend, #144612), PE anti-mouse CD3 (145-2C11 ...
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bioRxiv - Immunology 2023Quote: ... for 20 minutes at 4°C and stained in perm wash buffer with anti-IFNg PE or BV785 (ThermoFisher or BioLegend, clone: XMG1.2) for one hour at room temperature.
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bioRxiv - Cell Biology 2024Quote: S6K phosphorylation was measured by intracellular staining in murine CD8+ T cells activated for 24 h with plate-bound anti-CD3 (coated at 5 µg / ml for 14 h at 4 °C in PBS; BioLegend, Cat. # 100331) and 1 µg / ml soluble anti-CD28 (BioLegend ...
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bioRxiv - Microbiology 2024Quote: ... and 100 ng/ml Escherichia coli lipopolysaccharides (LPS) for 24 hrs to induce M1 polarization or 25 ng/ml IL-4 (BioLegend catalog #766202) and 25 ng/ml IL-13 (BioLegend catalog #571102 ...
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bioRxiv - Immunology 2024Quote: Staining for surface markers of immune cells was carried out in the dark at 4°C for 15 – 30 minutes using the following antibody panel: BV510-CD45 (clone 30F11; Biolegend, CA, USA), PE-Cy7-TCRβ (clone H57-597 ...
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bioRxiv - Immunology 2024Quote: ... cells were washed with 1x Perm buffer (Tonbo) and stained for intracellular antigens for 30 mins at 4°C with the following antibodies: GranzymeB: AF647 (Biolegend, Clone: GB11), IL-2 ...
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bioRxiv - Immunology 2023Quote: ... 7-aminoactinomycin-D (7-AAD; BioLegend), FAS-PEcy7 (DX2 ...
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bioRxiv - Developmental Biology 2023Quote: ... 7-aminoactinomycin D (7-AAD) (Biolegend) was used as death cell marker and added 15’ before the analysis ...
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bioRxiv - Immunology 2022Quote: ... 1:50 diluted anti-human CD32-APC (BioLegend, clone FUN-2), and 1:50 diluted anti-human CD64-PE/DAZZLE594 (BioLegend ...
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bioRxiv - Immunology 2022Quote: ... PE/Cyanine7 anti-mouse CD138 (Syndecan-1) (Clone 281-2, BioLegend), and the same anti-CD19 ...
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bioRxiv - Immunology 2023Quote: ... and 1 U/mL of recombinant human IL-2 (BioLegend, #589102) in 200 μL of complete culture medium (see above for media components) ...
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bioRxiv - Neuroscience 2024Quote: ... 2) anti-Aβ17-24 4G8 mouse monoclonal (Biolegend #800709 1:1000) and anti-tau (phospho-S262 ...
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bioRxiv - Cell Biology 2023Quote: ... Single cells were pelleted and re-suspended in a staining solution of DMEM containing 1% FBS and anti-Ki-67 Alex Fluor 647 (1:250 dilution in 3% BSA, Cat. No. 652407; BioLegend) for 1 hour on ice ...
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bioRxiv - Bioengineering 2022Quote: ... The next day samples were washed and incubated for 1h on shaker with DyLight™ 488 Donkey anti-rabbit IgG (minimal x-reactivity) Antibody (BioLegend 406404). After the incubation period ...
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bioRxiv - Immunology 2021Quote: ... P815 cells were used as target cells for human CTLs and pulsed for 1h at 37°C with 1µg/ml anti-CD3 antibody (clone UCHT1, Biolegend, Cat no. 300438). They were washed and resuspended at 1x105 cells/ml in killing assay media (RPMI without phenol red + 2% FCS ...
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bioRxiv - Cell Biology 2021Quote: ... were taken on day 1 and 3 and analyzed for TGFβ using ELISA assay (BioLegend) and IFNγ and TNFα using a multiplex-bead based assay (Meso Scale Discovery ...
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bioRxiv - Immunology 2021Quote: ... and for 3 h at 37°C with Brefaldin A (10 μg mL-1; Biolegend). Cell surfaces were stained and cells fixed as described above ...
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bioRxiv - Cell Biology 2023Quote: ... the cells were incubated with anti-SSEA-3 rat IgM antibody (1:1000, BioLegend, 330302) at 4°C for 1 h following incubation with fluorescein (FITC ...
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bioRxiv - Immunology 2022Quote: ... rIL-6 (20 ng/ml; Biolegend, #575706), rIL-23 (20 ng/ml ...
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bioRxiv - Immunology 2020Quote: ... rIL-6 (20 ng/ml; Biolegend, #575706), rIL-23 (20 ng/ml ...
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bioRxiv - Immunology 2022Quote: ... anti-IL-6-FITC (Biolegend, Cat# 501103), anti-IFN-γ-Alexa Fluor 700 (Biolegend ...
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bioRxiv - Immunology 2023Quote: ... anti-IL-6 (clone MP5-20F3, BioLegend), and isotype control (#RTK2071 ...
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bioRxiv - Microbiology 2024Quote: ... HLA-DR (G46-6; BioLegend, Cat# 562304), CD80 (2D10 ...
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bioRxiv - Cancer Biology 2023Quote: ... Hepatocytes were resuspended in 2 mL FACS buffer (2 mM EDTA, 0.5% BSA in PBS) and stained with Zombie Violet (1:500) (Biolegend #423113), TruStain FcX™ (anti-mouse CD16/32 ...