Labshake search
Citations for BioLegend :
801 - 837 of 837 citations for 5 Nitrofluorescein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Bioengineering 2024Quote: ... Blocking occurred using 5% BSA in TBST and antibody probing using 2% BSA in TBST at the indicated dilutions: α-pERK1/2 (Biolegend 369502) 5000x ...
-
bioRxiv - Immunology 2024Quote: ... PBMCs were stimulated as a single-cell suspension at a concentration of 4-5 x 106 cells/mL with the Cell Activation Cocktail (Biolegend, Cat # 423301) and incubated at 37°C with 5% CO2 ...
-
bioRxiv - Neuroscience 2024Quote: ... The following antibodies and channels were used to differentiate leukocyte populations CD4 BV510 (Biolegend Cat. No 100553, Clone RM4-5 0.038ng/µl), CD45 APC (Biolegend Cat ...
-
bioRxiv - Immunology 2024Quote: ... in a 5% CO2 incubator at 37 °C for 7 days with 30 ng/ml IL-15 (BioLegend, San Diego, CA, USA) and 10 ng/ml IL-12 (Peprotech ...
-
bioRxiv - Immunology 2021Quote: ... Nonspecific binding was blocked by incubation for 10 min at 4 °C with anti-CD16/CD32 antibody (Biolegend, clone 93, 5 ng/ μL) antibody and stained with the appropriate antibodies for 15 min at 4 °C ...
-
bioRxiv - Physiology 2019Quote: ... 100 μl cell suspension was transferred to the test tube and stained with 5 μl Pacific Blue Annexin V and propidium iodine (PI) solution (BioLegend, San Diego, CA) respectively for 15 min in dark ...
-
bioRxiv - Bioengineering 2022Quote: ... supernatant was removed and nanovials were reconstituted at a ten-fold dilution in Washing buffer containing 5 μl of 100 μg/mL anti-IFN-γ BV421 (Biolegend, Catalog: 502532), 25 μg/mL anti-TNF-α APC (Biolegend ...
-
bioRxiv - Bioengineering 2022Quote: ... Nanovials were pelleted at the last wash step and incubated with 5 μl of 100 μg/mL anti-IFN-γ BV421 (Biolegend, Catalog: 502532), 25 μg/mL anti-TNF-α Alexa Fluor 647 (Biolegend ...
-
bioRxiv - Developmental Biology 2022Quote: Single-cell suspensions were obtained as described above and subsequently blocked for 5-minutes on ice with Fc block CD16/32 (Biolegend, #101302, 1:100), followed by the addition of antibodies for 30-minutes on ice ...
-
bioRxiv - Immunology 2022Quote: Peripheral blood mononuclear cells from healthy donors were activated in cell culture plates precoated with CD3 (5 μg/mL, BioLegend, San Diego, CA) and CD28 (1 μg/mL ...
-
bioRxiv - Pathology 2019Quote: ... IL-9 and IL-6 determinations (Human IL-4, IL-5, IL-9 and IL-6 MAX, Biolegend, San Diego, CA 92121 USA) according to manufacturer’s protocol ...
-
bioRxiv - Immunology 2022Quote: Primary human neutrophils isolated as described were stained at room temperature for 20 minutes with 5 μM Calcein AM (BioLegend, Cat. No. 425201) and applied to the top well of a fluorescence-blocking 24-well transwell plate with 3 μm pores (Corning ...
-
bioRxiv - Immunology 2022Quote: ... Circulating maternal neutrophils were depleted at days 5 (E4.5) and 8 (E7.5) of pregnancy using a monoclonal neutralizing antibody (Biolegend, clone 1A8; 50 μg i.v.) or isotype control (Biolegend clone RTK2758 ...
-
bioRxiv - Microbiology 2024Quote: ... The panel of cytokines presented in Fig 5 was measured using the LEGENDplex™ Human Inflammation Panel 1 (13-plex) (BioLegend, Cat# 740809) kit ...
-
bioRxiv - Immunology 2024Quote: ... anti-mouse IL4 (10 µg/mL) and recombinant mouse IL2 (5 ng/mL) and IL12 (10 ng/mL) (BioLegend, San Diego, CA, US), for three days prior to use.
-
bioRxiv - Developmental Biology 2023Quote: ... 10 mg of protein G agarose beads were crosslinked to 5 μL of mouse anti-HA antibody (BioLegend, 901501, 1:1000, RRID: AB_2565006) using an effective concentration of 6.5 mg mL-1 DMP ...
-
bioRxiv - Immunology 2022Quote: ... Cells were centrifuged for 5 min at 500 x g and 4 °C and stained with Fc receptor blocker (TruStain fcX™, BioLegend, 1:100) and viability stain (Zombie Aqua™ Fixable Viability Kit ...
-
bioRxiv - Immunology 2023Quote: Biotinylated pneumococcal serotype 3 polysaccharide with a 5% biotin load was coupled with high concentration streptavidin-phycoerythrin (SA-PE) and streptavidin-allophycocyanin (SA-APC, both from BioLegend, San Diego, CA) in separate reactions ...
-
bioRxiv - Immunology 2024Quote: ... Each patient was also stained with one of TotalSeq™-C0251/2/3/4 Antibody (Biolegend, Cat: 394661/3/5/7, 1:100) for sample multiplexing ...
-
bioRxiv - Immunology 2024Quote: ... Cell pellets were resuspended in 100 µL of Fc-blocking medium (5 µg/ml Purified anti-mouse CD16/32 Antibody (Biolegend, San Diego, CA) in FACS buffer) ...
-
bioRxiv - Immunology 2022Quote: ... table 1) were diluted in blocking solution (F4/80 [Bio-Rad]: 10 µg/ml, Ly6G [abcam]: 1 µg/ml, MHC-II [BioLegend]: 5 µg/ml), CD163 [Invitrogen] ...
-
bioRxiv - Genomics 2021Quote: ... 5% normal goat serum in PBS for 1 hour and incubated at 4°C overnight with primary antibody (Biolegend, cat. 613402, dilution 1:500), then at room temperature for 3 hours with secondary antibody (Santa Cruz Biotechnology ...
-
bioRxiv - Cell Biology 2022Quote: ... half a million cells per sample were incubated for 10 min in 50 μl of PBS with 3% FSB and 5 μl of blocking buffer (BioLegend, San Diego, CA, USA) and then stained with 50x diluted TotalSeq-B cell feature barcode antibody (BioLegend ...
-
bioRxiv - Cancer Biology 2023Quote: ... 1.0 × 106 cells were incubated at room temperature for 20 min with 5 µL of APC-labeled anti-CD45 antibody (Clone: 2D1, BioLegend, San Diego, CA, USA). The cell lines were washed in PBS containing 2% FBS ...
-
bioRxiv - Cell Biology 2023Quote: ... Between 50,000 to 100,000 cells were resuspended in 10 uL of PBS supplemented with 2% FBS and 2 mM EDTA (hereafter named “FACS buffer”) together with 5 uL of TruStain FcX (BioLegend, San Diego, CA, USA). Cells were incubated with the FcX block for 10 minutes at RT ...
-
bioRxiv - Cancer Biology 2023Quote: ... Cells were stained for one hour using the following antibodies: Allophycocyanin (APC) anti-human CD274 (5 μL/test; BioLegend #329707; BioLegend, San Diego, CA, USA), APC anti-mouse CD274 (5 μL/test ...
-
bioRxiv - Microbiology 2023Quote: ... the FOXP3/Transcription Factor Staining Buffer kit (eBioscences – 00-5523-00) was used along with the following anti-mouse antibodies: BV605-conjugated anti-CD4 (Biolegend, RM4-5; 1/700), BV650-conjugated anti-B220 (BD Biosciences ...
-
bioRxiv - Cancer Biology 2024Quote: ... cells were permeabilized with 0.1% Triton X-100 in PBS (5 min at RT) and immunolabeled with anti-HA (Biolegend, 901501; 1:500 in PBS) for 1 hr at RT ...
-
bioRxiv - Genetics 2023Quote: ... The barcoded cells were then collected into a single tube and stained with a cocktail of antibodies: CD33 PECy7 (5 µL per 100 µL stain, Biolegend, cat#303434, clone WM53) and pSTAT3 AlexFlour488 (2.5 µL per 100 µL stain ...
-
bioRxiv - Systems Biology 2023Quote: ... The resulting single cells suspension was used to detect leukocyte populations (CD45 BV421 [clone: 104; Biolegend], CD4 PerCPeFluor710 [RM4-5; eBioscience], CD8a APC-Cy7 [53-6.7; Biolegend], CD19 PE-Cy7 [6D5; Biolegend]) and CD4+ Foxp3+ Treg (CD45 BV421 ...
-
bioRxiv - Neuroscience 2023Quote: C57Bl/6J or LysMGFP mice were injected intravenously with 5 µg of AF647 conjugated to anti-Ly6G antibody (127610; Biolegend, San Diego, CA USA) 3 hours before imaging ...
-
bioRxiv - Neuroscience 2024Quote: ... Supernatant was discarded and samples were blocked in 50 µl FACS buffer containing CD16/CD32 (Biolegend Cat No. 101301, Clone 93, 5 ng/µl) for 10 minutes ...
-
bioRxiv - Systems Biology 2023Quote: ... All iPSCs were stained in PBS with 5% FBS overnight at 4°C with the following antibodies and dilutions: Alexa Fluor® 647 anti-tubulin α (1:1000, Biolegend, #627908, clone 10D8), and Alexa Fluor ® 488 anti-Oct-4A (1:500 ...
-
bioRxiv - Cancer Biology 2023Quote: ... 10 ng/ml TNF-α in 5 μg/ml CHX and 20 ng/ml TNF-α in 10 μg/ml CHX of TNF-α (Biolegend, United States) for 24h treatments were performed in varying concentrations ...
-
bioRxiv - Cancer Biology 2023Quote: ... Cells were stained for one hour using the following antibodies: Allophycocyanin (APC) anti-human CD274 (5 μL/test; BioLegend #329707; BioLegend, San Diego, CA, USA), APC anti-mouse CD274 (5 μL/test ...
-
bioRxiv - Neuroscience 2023Quote: ... The blocking of nonspecific binding was induced in the cell suspensions via incubation for 10 min at 4°C with anti–CD16/CD32 antibody (cat # 101320, BioLegend, clone 93, 5 ng/μl). This was followed by staining for 30 min at 4°C with the appropriate antibodies ...
-
bioRxiv - Immunology 2024Quote: ... cells were centrifuged at 900 x g for 5 minutes at room temperature and stained with Zombie Aqua Fixable viability kit (Biolegend, 1:1000 diluted in PBS, 100µL) for 20 minutes at room temperature in the dark to enable exclusion of dead cells ...