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Citations for BioLegend :
801 - 850 of 7858 citations for 1 2 Bis Pentabromophenyl Ethane Unlabeled 25 Ug Ml In Toluene since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: Mice were injected retro-orbitally with 25 µg of a fluorophore-conjugated antibody raised again VE-cadherin (BV13; Biolegend) 15 minutes prior to sacrifice ...
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bioRxiv - Cell Biology 2020Quote: ... mice were injected retro-orbitally with 25 µg of a fluorophore-conjugated antibody raised again VE-cadherin (BV13; Biolegend) 15 minutes prior to sacrifice ...
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bioRxiv - Cancer Biology 2022Quote: Mice were injected intravenously (i.v.) with 250 μl of a solution containing 25 μg anti-CD107a PE (ID4B, Biolegend) and 10 μg monensin (Biolegend ...
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bioRxiv - Immunology 2019Quote: ... OVA presentation was confirmed by flow cytometry using a PE-labeled antibody against surface SIINFEKL bound to H-2Kb (clone 25-D1.16, BioLegend).
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bioRxiv - Immunology 2020Quote: ... OVA presentation was confirmed by flow cytometry using a PE-labeled antibody against surface SIINFEKL bound to H-2Kb (clone 25-D1.16, BioLegend).
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bioRxiv - Microbiology 2023Quote: ... for 1h at 25°C and then transferred to TBST supplemented with purified anti-HA antibody clone 16B12 (Biolegend) at a 1:2000 dilution for 1h at 25°C ...
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bioRxiv - Cell Biology 2021Quote: ... the concentration of IL-2 in the supernatant was measured using an IL-2 ELISA kit (Biolegend).
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bioRxiv - Immunology 2022Quote: ... IL-2 concentration in harvested supernatant was then analyzed using Human IL-2 ELISA Max Kit (BioLegend) per provided instruction ...
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bioRxiv - Immunology 2023Quote: ... and interleukin-2 (IL-2) and interferon-ψ concentrations were determined by enzyme-linked immunosorbent assay (BioLegend). Triplicate wells were plated for each condition.
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bioRxiv - Immunology 2019Quote: PBMCs were seeded in a 24 well plate at 2 × 106 cells/well in RPMI-1640 with anti-CD107a antibody (Biolegend, 1:200). Then ...
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bioRxiv - Cancer Biology 2021Quote: ... 10 μL of CytoC stain buffer (2% Tween20, 10% BSA (w/v) in PBS) + 1:400 Alexa Fluor 647 anti-cytochrome c antibody (Biolegend, cat#612310) + 1:100 DAPI (1 mg/mL ...
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bioRxiv - Immunology 2022Quote: ... lymph node FNA samples collected 2 or 8 weeks post-boost were stained for 30 min on ice with purified CD16 (3G8, BioLegend, 1:100), CD32 (FUN-2 ...
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bioRxiv - Bioengineering 2023Quote: ... and stained with the following antibodies in FACS staining buffer (PBS with 2% Fetal Bovine Serum, 1% Penicillin-Streptomycin): anti-human CD31 (BioLegend, WM59, FITC), anti-human CD45 (BioLegend ...
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bioRxiv - Immunology 2023Quote: ... Cells were suspended in PBS + 2% HI-FBS + 2mM EDTA in the presence of Fc receptor blocking antibody (BioLegend, 101302, 1:400) and stained with antibodies at a 1:400 dilution against the following mouse markers ...
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bioRxiv - Bioengineering 2023Quote: ... The slides were then incubated in the primary antibodies (rat anti-PECAM-1, clone 390, EMD Millipore; rabbit anti-NG-2 chondroitin sulfate, EMD Millipore; rabbit anti-Keratin-14, BioLegend) diluted 1:100 in the blocking solution overnight at 4 °C ...
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bioRxiv - Immunology 2023Quote: ... Cells were washed and incubated in FACS buffer (1xPBS, 2%FCS) with the following anti-human antibodies: CD19-BV785 (BioLegend, 1:10), CD3-APC-eFluor 780 (Invitrogen ...
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bioRxiv - Immunology 2022Quote: ... activated with SIINFEKL (63ng/ml - 500ng/ml) or plate-bound anti-CD3 (1μg ml, clone 145-2C11, Biolegend) and anti-CD28 (1μg/ml ...
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bioRxiv - Immunology 2023Quote: ... 0.5µg/mL) and ionomycin (1µg/mL) and for 3h at 37°C with brefaldin A (10µg/mL; Biolegend). Cell surfaces were stained ...
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bioRxiv - Neuroscience 2019Quote: ... Comparable amounts of enzymatically active γ-secretase and mature γ-secretase between different assay conditions were confirmed using mouse monoclonal anti-presenilin 1 (Biolegend, SIG-39194, 1 µg/ml) and rabbit polyclonal anti-nicastrin (Sigma ...
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bioRxiv - Molecular Biology 2021Quote: CH12 cells were stimulated to undergo CSR to IgA by treatment with 1-5 μg/mL α CD40 (BioLegend), 5 ng/ml TGF (R&D Systems ...
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bioRxiv - Cancer Biology 2019Quote: ... Purified cells were plated at 1×105 cells per well on a 96 well plate coated with 10μg/ml αCD3 (Biolegend), and cultured with 2μg/ml soluble αCD28 (Biolegend) ...
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bioRxiv - Cell Biology 2021Quote: 1×106 (for immunoblot) or 1.8×107 (for immunoprecipitation) Jurkat Cells were incubated with anti-CD3 (1 μg/ml, OKT3, 317325, BioLegend) and anti-CD28 (1 μg/ml ...
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bioRxiv - Immunology 2019Quote: ... OVA-specific IgG1 was detected using biotinylated anti-mouse IgG1 monoclonal antibody (0.02µg/mL RMG1-1, Biolegend – CA, USA) followed by SA-HRP (1:2000 dilution #P030701-2 ...
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bioRxiv - Cell Biology 2020Quote: ... and DAPI/conjugated PECAM1 antibody (0.04 µg/ml DAPI, Sigma, 10236276001; 1:250 Alexa647-anti-human PECAM1, Biolegend, 303112) in blocking buffer for 1 hr each ...
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bioRxiv - Immunology 2021Quote: ... and seeded in the antibody-coated flask at 3–5×105 cells/cm2 together with soluble 1 µg/mL anti-CD28 antibodies (clone 37.51; 102116, BioLegend). Cells were cultured for 48 hr at 37°C in a humidified 5% CO2 atmosphere ...
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bioRxiv - Cell Biology 2022Quote: ... poly-l-lysine coated glass slides were coated by overnight incubation with ICAM-1 (5 μg/mL, Biolegend 552906) or with ICAM-1 and anti-CD28 (5 μg/mL ...
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bioRxiv - Immunology 2022Quote: ... and then stained for surface antibodies at .1 μg/mL unless indicated: NK1.1 PerCP-Cy5.5 (clone PK136, BioLegend 10727), F4/80 AF594 (clone BM8 ...
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bioRxiv - Immunology 2022Quote: ... Followed by ACE2-biotin at 1 μg/mL (RnD Systems) incubation for 30 minutes and then SA-APC (Biolegend) incubation for 1 hour ...
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bioRxiv - Immunology 2023Quote: ... They were cultured in Th1-polarising media consisting of R10 with 1 μg/mL anti-mouse CD28 (102101, BioLegend), 5 μg/mL anti-mouse IL-4 (504102 ...
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bioRxiv - Immunology 2023Quote: ... were coated with 1 μg/mL capture antibodies (IFNγ: clone R4-6A2, BD Pharmingen; or IL-17A: clone TC11-18H10.1, Biolegend) diluted in carbonate buffer ...
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bioRxiv - Biochemistry 2023Quote: ... Naïve CD4+ T cells were then activated with anti-CD3 (Biolegend, Cat#100340, 1:500 dilution to 5μg/mL), anti-CD28 (Biolegend ...
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bioRxiv - Immunology 2023Quote: ... were given by tail-vein intravenous (i.v.) injection in 200 μL of PBS with 1 μg/mL of anti-CD3 antibody (OKT3, Biolegend). Monthly post HPC transplant ...
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bioRxiv - Neuroscience 2024Quote: ... Several positive controls were utilised: (1) polyclonal T-cell simulation with soluble αCD3 (0.5 μg/ml; clone OKT3; Biolegend) and α-CD28 (1μg/ml ...
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bioRxiv - Cancer Biology 2024Quote: ... Cells were washed three times with Cell Staining Buffer and stained with Alexa Fluor 647 anti-human Ki-67 antibody (1:100) and DAPI (0.5 µg/ml) respectively in 1x Intracellular Staining Permeabilization Wash Buffer (BioLegend). The cells were resuspended in cell staining buffer (BioLegend ...
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bioRxiv - Cancer Biology 2024Quote: ... cells were washed with FACS-wash (FW-1% FBS in PBS) in 96-well V-shape plates and stained with DAPI (0.1 µg/ml) (Biolegend) for 5 minutes ...
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bioRxiv - Immunology 2022Quote: ... 20 ng/ml IL-4 + 20 ng/ml IL-13 (Biolegend) for polarization to M2 ...
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Cell therapy with IL-10-producing group 2 innate lymphoid cells suppresses Graft-versus-Host diseasebioRxiv - Immunology 2023Quote: ... 20ng/mL IL-7 and 20 ng/mL IL-33(BioLegend).
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bioRxiv - Immunology 2022Quote: ... in the presence of plate-bound anti-CD3 (1 μg/ml) and soluble anti-CD28 (0.5 μg/ml) supplemented or not with IL-27 (30 ng/ml, BioLegend). Proliferation of Tconv cells was determined by CellTrace Violet Dye dilution in FACS analysis ...
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bioRxiv - Biophysics 2021Quote: ... DC were stained with PE CD45 (30-F11) and APC CD11c (N418) for 25 minutes at 4°C (all Biolegend). Excess antibodies were removed by washing with HBSS + 0.1% BSA ...
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bioRxiv - Systems Biology 2020Quote: ... The cells were incubated with each antibody for 45 min at −22–25°C after FcR blocking using Fc blocker (Human TruStain FcX, Biolegend) or anti-mouse CD16/CD32 antibody ...
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bioRxiv - Microbiology 2021Quote: ... Plates were washed with water three times before applying 25 μL of secondary antibody (goat anti-mouse IgG Fc, BioLegend) at a dilution of 1:4,000 in blocking solution ...
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bioRxiv - Genomics 2020Quote: ... 1.25 × 10^6 PBMCs were spun down and resuspended in 25 μl CITEseq antibody cocktail (TotalSeq™-A Antibodies, BioLegend) containing among others one hashing antibody per each sample ...
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bioRxiv - Cell Biology 2020Quote: ... C57BL6 mice were injected retro-orbitally with 25 µg of a fluorophore-conjugated antibody raised again VE-cadherin (BV13; Biolegend) 15 minutes prior to sacrifice ...
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bioRxiv - Immunology 2023Quote: ... CD8+ T cells were activated in 96-wells culture plates (25 x103 cells/well) coated with anti-CD3 and anti-CD28 (Biolegend) or by the addition of the DPP-8/9 inhibitor Talabostat (Val-boroPro ...
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bioRxiv - Cancer Biology 2023Quote: ... Viability of BMNC (0.25 x106) was assessed by Annexin V and 7-AAD staining (APC Annexin V Apoptosis Detection Kit 7-AAD, Biolegend) using FCM.
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bioRxiv - Immunology 2023Quote: ... were stained on ice for 25 minutes with the following panel of lineage-specific antibodies: Biotin-labeled TER-119 (TER-119, BioLegend), Biotin-labeled GR-1 (RB6-8C5 ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Cells were counted and then incubated at 4 °C for 25 min with FITC anti-mouse CD45 (Biolegend, 103107, USA), Brilliant Violet 650 Ly6G (Biolegend ...
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bioRxiv - Immunology 2021Quote: ... anti-IgD-APC-Cy7 (Biolegend, IA6-2), anti-CD38-PE-Cy7 (Biolegend ...
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bioRxiv - Immunology 2022Quote: ... anti-CD32-PE-Cy7 (FUN-2, Biolegend), anti-HLA-DR-BV711 (L243 ...
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bioRxiv - Immunology 2022Quote: ... anti-CD32-PE-Cy7 (FUN-2, Biolegend) and ...