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Citations for BioLegend :
8101 - 8150 of 10000+ citations for Rabbit Anti Human IgG Fc Biotin since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2021Quote: ... PBMCs were collected and stained using APC/Fire anti-CD4 and APC anti-CD8 (Supplemental Table 1) (Biolegend, San Diego CA). PBMCs were first washed and stained with Zombie Yellow (Supplemental Table 1 ...
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bioRxiv - Cell Biology 2021Quote: NK92 and YTS cell lines were incubated for 20 mins at 37°C and 5% CO2 on #1.5 glass coverslips that had been pre-coated with anti-CD18 (clone IB4, 10 μg/ml) and 10 μg/ml anti-NKp30 (clone P30-15, Biolegend, NK92) or anti-CD28 (BD Biosciences ...
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bioRxiv - Cell Biology 2021Quote: ... WT and CD56 KO NK92 and YTS cells were incubated for 20 mins at 37°C and 5% CO2 on .5 glass coverslips that had been pre-coated with 10 μg/ml anti-CD18 (clone IB4) and anti-NKp30 (clone P30-15, Biolegend, NK92) or anti-CD28 (clone CD28.2 ...
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bioRxiv - Immunology 2021Quote: ... permeabilized with saponin 0.2% for 20 min and stained with PE-Cy7-anti-IFN-γ mAb and/or PE-anti-IL-10 mAbs (Biolegend). For TF and GzB/PRF staining ...
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bioRxiv - Cell Biology 2020Quote: ... anti-CD9 (clone VJ1/20.3.1; kindly provided by Francisco Sánchez, Madrid, Spain) and anti-CD63 (H5C6; BioLegend, San Diego, CA, USA). Band intensities were analysed using Image J.
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bioRxiv - Immunology 2022Quote: ... for 30min at 4 °C and intracellular markers (BV421-labeled anti-mouse IFN-γ, Cat# 505830, BioLegend; FITC-labeled anti-mouse IL-2, Cat# 503806, BioLegend; BV711-labeled anti-mouse TNF-α ...
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bioRxiv - Immunology 2022Quote: ... mouse anti-human CD47 antibody conjugated to APC (323123) and anti-HA Alexa Fluor® 488 anti-HA.11 Epitope Tag (901509) were purchased from Biolegend. The antibody to ORF3a was a gift from Prof ...
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bioRxiv - Immunology 2020Quote: ... anti-CD45.1 (Clone A20, Catalog # 110728), and anti-CD45.2 (Clone 104, Catalog # 109808) antibodies were obtained from Biolegend (San Diego, CA) for analysis of mouse immune profiles.
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bioRxiv - Cancer Biology 2022Quote: ... Cells staining positive for anti-CD11b-Pacific blue (Miltenyi, 130-110-802) and anti-F4/80-APC Cy7 (Biolegend. cat.123117) were sorted as macrophages ...
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bioRxiv - Cell Biology 2022Quote: ... 45-5981-82), hamster anti-mouse CD48 APC (eBioscience, 17-0481-82) and rat anti-mouse CD150 PE-Cy7 (Biolegend, 115914). To examine macrophages ...
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bioRxiv - Molecular Biology 2024Quote: ... Western blotting was performed using an anti-HA mouse monoclonal antibody (Alexa Fluor® 488 anti-HA.11 Epitope Tag Antibody, BioLegend) as primary antibody diluted 1:1000 in TBS-T (20 mM Tris ...
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CD56/NCAM mediates cell migration of human NK cells by promoting integrin-mediated adhesion turnoverbioRxiv - Cell Biology 2023Quote: ... at 37°C and 5% CO2 on #1.5 glass coverslips that had been pre-coated with 10 µg/ml anti-CD18 (clone IB4) and anti-NKp30 (clone P30-15, Biolegend; 325202) or 5 µg/ml Fc-ICAM-1 (Enzo Life Science ...
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bioRxiv - Microbiology 2023Quote: ... and 20 minute incubation with primary antibody cocktail (CD45+ immune cells: 1:50 anti-CD45-VioGreen [Miltenyi Biotec, Germany]; CD31+ endothelial cells: 1:2000 anti-CD31-PE [BioLegend, CA]/1:50 anti-CD45-VioGreen [Miltenyi Biotec ...
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bioRxiv - Microbiology 2023Quote: ... The membrane was incubated in 1% milk-PBS-T containing rabbit anti-LpxC antibody (a generous gift from the Doerrler lab) or mouse anti-RpoA (anti-E.coli RNA polymerase alpha from Biolegend, cat# 663104) diluted 1:10,000 ...
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bioRxiv - Neuroscience 2023Quote: ... Membranes were incubated with primary anti-HA antibodies diluted in PBS-tween 0.2% blocking medium (mouse anti-HA, 1:5000, Biolegend cat. # 901513) over night at 4°C under agitation ...
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bioRxiv - Genetics 2023Quote: ... Recombinant Anti-AKT1 + AKT2 + AKT3 (phospho S472 + S473 + S474, Purified anti-Tubulin β-3 (TUBB3) Antibody 1:1000 (Biolegend, # PRB-435P), anti-GAPDH antibody 1:1000 (Sigma Aldrich ...
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bioRxiv - Neuroscience 2023Quote: ... non-fat dried skimmed milk powder and then probed with ICSM35 anti-PrP antibody (D-Gen Ltd; 1:5000 for RML) or with 6D11 anti-PrP antibody (BioLegend (808004); 1:5000 for ME7 ...
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bioRxiv - Biophysics 2023Quote: ... biotinylated anti-mouse CD3e (Cat# 100304) and FITC anti-mouse TCRb antibody (Cat# 109206) were purchased from BioLegend (San Diego, CA). Azide-PEG4-NHS ester (Cat# AZ103-100 ...
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Cancer-associated fibroblasts produce matrix-bound vesicles that influence endothelial cell functionbioRxiv - Cancer Biology 2023Quote: ... cells were resuspended in FACS buffer and incubated with Alexa Fluor® 488 anti-CD31 ([390] 102414) and APC/Cyanine7 anti-CD45 ([30-F11] 103116) antibodies (1/100, BioLegend) and mouse TruStain FcXTM (1/200 ...
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bioRxiv - Bioengineering 2023Quote: ... staining was performed for 30 minutes at 4 C with both 70 nM AlexaFluor647-conjugated HER2 and 1ng/μL anti-DYKDDDDK (anti-FLAG) Tag Antibody-PE (Biolegend, 637310). The 5A12 library was first incubated with either 0.5 nM VEGF (Acro ...
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bioRxiv - Immunology 2024Quote: ... in FACS buffer and stained with anti-hCD19-PE-Cy7 and anti-hCD138-PE-Cy7 (Biolegend, #302216 and #356513, 1:100). After washing ...
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bioRxiv - Neuroscience 2023Quote: ... brains were incubated with 1:1000 chicken anti-GFP antibody (Aves GFP-1020) and 1:1000 mouse anti-tyrosine hydroxylase antibody (BioLegend 818001), with boosters over 5 days for a final concentration of 1:200 ...
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bioRxiv - Cell Biology 2024Quote: ... were from Santa Cruz Biotechnology. Mouse anti-β-tubulin (E7) was from the Hybridoma bank. Mouse anti-Nup153 (clone QE3, cat. 906201) was from Biolegend. Mouse anti-GAPDH (clone 6C5 ...
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bioRxiv - Genomics 2024Quote: ... hfCas13 knockdown activity was confirmed by flow cytometry using APC anti-CD46 and FITC anti-CD55 (BioLegend 352405 and 311306, respectively). Monoclonal doxycycline-inducible RfxCas13d-NLS HEK293FT ...
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bioRxiv - Immunology 2024Quote: ... PE-Cyanine7-conjugated anti-mouse Ly6G (E-AB-F1108H, elabscience, China) or intracellular staining for 488-conjugated anti-mouse IL-17A (506910, BioLegend, USA) was performed according to the manufacturer’s instructions.
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bioRxiv - Cell Biology 2019Quote: ... Mouse anti-β-III-tubulin antibody TUJ1 (801202) and mouse anti-neurofilament monoclonal antibody SMI312 (837904) were from Biolegend (San Diego, CA). Mouse anti-α-tubulin antibody (DM1A ...
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bioRxiv - Immunology 2019Quote: ... PD-L1 and CD80 were stained by anti-PD-L1 PE (eBioscience, catalog 14-5983-82) and anti-CD80 PE (Biolegend, catalog 305208) and their expression levels were quantified using the QUANTUM™ R-PE MESF kit (Bangs Laboratories Inc ...
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bioRxiv - Neuroscience 2021Quote: ... The exception is that secondary labeling was accomplished using anti-rat DyLight 405 (1:5000, Novus, NBPI-72983) or anti-mouse DyLight 405 (1:1000, BioLegend, Poly-24091). Fixed cells were then imaged on a Nikon Eclipse TE2000 inverted microscope using a 60x 1.49NA oil-immersion TIRF objective and 405nm and 560nm solid lasers using a CoolSNAP HQ2 CCD camera ...
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bioRxiv - Immunology 2021Quote: ... PE-conjugated anti-mouse TNF-α (clone MP6-XT22) and Pe-Cy7–conjugated anti-mouse IL-2 (clone JES6-5H4) antibodies (BioLegend, UK) for 20 min at 4°C ...
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bioRxiv - Neuroscience 2022Quote: ... mouse anti-myelin-associated glycoprotein (MAG, 1:100, SigmaAldrich MAB1567),chicken anti-glial fibrillary acidic protein (GFAP, 1:500, BioLegend PCK-591P), chicken anti-GFAP (1:500 ...
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bioRxiv - Immunology 2022Quote: ... anti-Pan-gδ PE-Vio770 (clone 11f2; Miltenyi Biotech, Bergisch Gladbach, Germany) and anti-Vδ2 BV711 (clone B6; Biolegend, San Diego, CA) for 60 minutes at room temperature ...
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bioRxiv - Pathology 2024Quote: ... then incubated with primary antibody anti-IBA1 (Wako; 011-27991; dilution 1:100) and anti-Amyloid Beta1-42 (Biolegend; 805501; 1:500) prepared in 1% BSA for overnight at 4°C ...
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bioRxiv - Immunology 2024Quote: ... PE-conjugated anti- mouse IFN-γ (XMG1.2) and PerCP-Cy5.5-conjugated anti-mouse T-bet (4B10) were purchased from BioLegend (San Diego, CA).
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bioRxiv - Synthetic Biology 2023Quote: ... OD600 of induced cultures was taken and used to determine approximate volumes of culture to stain two million cells with fluorescently labeled antibodies (anti-FLAG PE, Biolegend, cat# 637309, anti-HA Alexa 647, Biolegend, cat #682404). Cells were washed in PBS 0.5% BSA (0.5% BSA ...
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bioRxiv - Microbiology 2020Quote: ... and/or monoclonal anti-RNA polymerase (1:1,2000, Biolegend). Western blots were probed with IRDye-conjugated secondary antibodies and visualized with Odyssey CLx imagining system (Li-COR Biosciences ...
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bioRxiv - Cancer Biology 2021Quote: ... and 1 µg of anti-CD45RO (clone UCHL1, BioLegend) antibodies ...
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bioRxiv - Cancer Biology 2021Quote: ... and anti-PD-1(CD279)-PE/Cy7 (BioLegend 135216). The myeloid antibody cocktail contained ...
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bioRxiv - Cancer Biology 2021Quote: ... Anti-TNF neutralizing antibody (#506325) was obtained from Biolegend.
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bioRxiv - Cell Biology 2020Quote: ... anti-HA (mouse; clone 16B12; 901501; Biolegend;1:500), streptavidin-HRP (Pierce-21140;Thermo Scientific ...
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bioRxiv - Cell Biology 2020Quote: ... rat anti CD41 Alexa Fluor 647 conjugated (133934, Biolegend), and mouse anti IL1β (12242 ...
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bioRxiv - Cell Biology 2019Quote: ... mouse monoclonal anti-keratin 8 (BioLegend, 904801; 1:200), and mouse monoclonal anti-β-casein (Santa Cruz ...
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bioRxiv - Cell Biology 2020Quote: Mouse anti-beta-actin antibody (clone 2F1-1, Biolegend)
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bioRxiv - Immunology 2021Quote: ... Armenian hamster anti-mouse CD80 (16-10A1, 104729 Biolegend) and rabbit antimouse p-NF-KB p65 (Ser536 ...
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bioRxiv - Immunology 2021Quote: ... and stained with anti-TNFα–BV421 (clone MAb11, BioLegend) and anti-IFNγ–APC (clone 4S.B3 ...
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bioRxiv - Immunology 2021Quote: ... with either 10 μg anti-DR3 antibody (4C12, Biolegend) or 10 μg Armenian Hamster IgG control antibody (HTK888 ...
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bioRxiv - Immunology 2021Quote: ... Pellets were suspended and incubated with anti-CD62L (BioLegend), anti-CD127 (BioLegend) ...
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bioRxiv - Developmental Biology 2021Quote: ... Mouse anti-HA (1:1,000, 16B12, MMS-101P, BioLegend) and mouse anti-Myc (1:1,000 ...
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bioRxiv - Neuroscience 2021Quote: ... and incubated with mouse anti-Tuj1 (1:500; BioLegend) overnight at 4°C ...
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bioRxiv - Molecular Biology 2021Quote: ... and PE-conjugated anti-mouse IL1a antibody (clone:ALF161, BioLegend) containing 500 μl FACS buffer ...
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bioRxiv - Immunology 2022Quote: ... anti-TIM-3 (BV 711, clone F38-2E2 – BioLegend), anti-PD1 (PE-Cy7 ...