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Citations for BioLegend :
751 - 800 of 914 citations for Recombinant Rat Lgals1 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2023Quote: ... zoledronic acid (Novartis Pharmaceuticals) and/or 50 μg/kg (i.p.) recombinant human granulocyte-colony stimulating factor (G-CSF; carrier-free, BioLegend #578604) for three days ...
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bioRxiv - Immunology 2023Quote: ... Cells were incubated at 37°C and 5% CO2 for one hour to settle and then stimulated with 20 ng/mL recombinant IFNg (BioLegend), 100 ng/mL LPS-EB Ultrapure (Invivogen ...
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bioRxiv - Cancer Biology 2023Quote: ... T cells were incubated for 48h with OVAp (10 ng/ml) and mouse recombinant IL2 (50 U/ml) (Cat# 575402, Biolegend), was added for a period of five additional days ...
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bioRxiv - Cancer Biology 2024Quote: ... T cells were activated with αCD3/αCD28 Dynabeads as specified by manufacturer’s instructions in the presence of 100 IU/mL recombinant mouse (rm) IL-2 and 10 ng/mL rmIL-7 (BioLegend) and cultured in RPMI1640 supplemented with 10% FBS ...
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bioRxiv - Immunology 2023Quote: ... 200 μl for 96-well plates) supplemented with 1 μg/ml of recombinant human B cell-activating factor (BAFF, BioLegend). 1 µg/ml rHA from H1N1 (PR8 ...
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bioRxiv - Immunology 2024Quote: ... were cultured for 3 days in 96-well U bottom plates coated with 2 μg/mL anti-CD3 (eBioscience) and 1 μg/mL anti-CD28 (eBioscience) supplemented with 10ng/mL of recombinant mIL-2 (Biolegend) to allow Treg survival ...
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bioRxiv - Cancer Biology 2019Quote: ... was from BioXCell (Lebanon, NH), and rat anti-murine CD3 (Cat no 100331, LEAF purified) from Biolegend (San Diego, CA).
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bioRxiv - Developmental Biology 2021Quote: ... rat anti-mouse Ly-6A/E (Sca1) (labelled with BV510) (BioLegend – 108129; 5 µl per million cells) and rat anti-lineage cocktail (labelled with BV421 ...
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bioRxiv - Cancer Biology 2022Quote: ... was used as the primary antibody and rat anti-human IgG Fc antibody conjugated to APC (BioLegend) was used as the secondary antibody ...
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bioRxiv - Immunology 2019Quote: ... Fc-Block was performed (10µg/ml purified rat anti-mouse CD16/CD32, Biolegend TruStain FcX anti-mouse), after which cells were stained with Fixable Viability Staining e780 (1:1000 ...
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bioRxiv - Immunology 2019Quote: ... anti-rat IgG1/2a/2b/2c-Biotin combined with Streptavidin-HRP Streptavidin-HRP (all BioLegend, London, UK).
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bioRxiv - Neuroscience 2019Quote: ... and cells were stained with rat anti-mouse ICAM-1 (1:100; Biolegend YN1/1.7.4 in PerCP), rat anti-mouse CD45 (1:100 ...
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bioRxiv - Microbiology 2022Quote: ... Cells were washed with PBS5 and stained with APC-conjugated goat anti-rat IgG (Biolegend, 1:200) for 20 min ...
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bioRxiv - Developmental Biology 2021Quote: ... single-cell suspensions were incubated 20min on ice in 500µL FACS buffer containing 3.5µg/mL of rat anti-mouse EpCAM antibody conjugated with Alexafluor 647 (Biolegend) and 8µg/mL mouse anti-β2-Microglobulin monoclonal antibody conjugated with PE (Santa Cruz) ...
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bioRxiv - Molecular Biology 2021Quote: ... incubated with secondary antibody diluted in 3% BSA (Alexa Fluor 594 Goat anti-Rat IgG, BioLegend, #405422) in the dark for 1 hr at room temperature ...
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bioRxiv - Immunology 2020Quote: ... the same lysates were processed using LEGENDplex™ Mouse/Rat Free Active/Total TGF-β1 Assay (BioLegend) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... rat anti-mouse CD68 in FITC at 1:300 for 30 minutes (BioLegend®, Cat. No. 137005) using Intracellular Fixation & Permeabilization Buffer (eBioscience ...
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bioRxiv - Microbiology 2023Quote: ... GC B cells were labeled using rat anti-GL7 antibody (FITC; BioLegend, catalog no. 144604, 1:250). Tfh cells were labeled using anti-CD4 antibody (BioLegend ...
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bioRxiv - Cancer Biology 2023Quote: ... or Anti-human/mouse CD49f antibody (#313620, Biolegend) (isotype control, Pacific Blue Rat IgG2a, κ; #400527, Biolegend). For GD3 FACS analysis ...
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bioRxiv - Immunology 2023Quote: ... Alexa Fluor 647 or FITC–conjugated rat anti-mouse CD326 (Ep-CAM) antibody(1:200; Biolegend, G8.8), PE/Cyanine5 anti-mouse CD86 antibody(1:200 ...
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bioRxiv - Microbiology 2023Quote: ... samples were treated with rat anti-mouse CD16/32 antibody (1:1000, clone 93, BioLegend ref. 101302) at 4°C for 15 min to block Fc receptors ...
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bioRxiv - Developmental Biology 2023Quote: The following antibodies were used for FACS: PE/Cy7 rat anti-CD45 (CD45, 1:250, 103114, BioLegend), PE rat anti-epithelial cadherin (ECAD ...
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bioRxiv - Immunology 2023Quote: ... The following antibodies were used for labeling cells: PE rat anti-mouse CD45 (Clone 30-F11; BioLegend), FITC rat anti-mouse CD3 (Clone 17A2 ...
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bioRxiv - Immunology 2023Quote: ... for further 20 min on ice with the following antibodies: rat anti-mouse CD19 PE (Biolegend, 115507), rat anti-mouse CD138 FITC (Thermo Fisher ...
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bioRxiv - Cancer Biology 2021Quote: ... mice were anaesthetised as described above and intranasally inoculated with 100ng of carrier free recombinant protein (rIL-1α – Biolegend #575002, rIL-1β – Biolegend #575102) suspended in sterile phosphate buffered saline (PBS) ...
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bioRxiv - Molecular Biology 2020Quote: ... Organoids were cultivated in growth medium as previously described (61) with minor changes (50% mouse Wnt3a-conditioned medium, 50ng/mL mouse Wnt3a recombinant protein (Biolegend, USA), 15% mouse R-spondin1-conditioned medium ...
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bioRxiv - Cancer Biology 2019Quote: ... at concentration 2×10^6/ml in IMDM containing 10% FBS and 100U/ml of recombinant mouse IL-2 (BioLegend, CA). Cells were cultured for up to 7 days and used for functional experiments in vitro and in vivo.
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bioRxiv - Cancer Biology 2019Quote: ... The cells were washed with HBSS twice by centrifugation at 250 x g for 8 min and then suspended in BMDC culture medium (RPMI-1640 medium [Thermo Fisher Scientific] containing 10% FBS, 20 ng/ml recombinant mouse GM-CSF [BioLegend] ...
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bioRxiv - Cell Biology 2021Quote: ... non-adherent cells were moved to bacteriological Petri dishes the next day and differentiated over 1 week in cDMEM containing 20 ng/mL recombinant murine M-CSF (Biolegend, 576404). Generation of murine bone-marrow derived dendritic cells (BMDC ...
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bioRxiv - Bioengineering 2022Quote: ... and resuspended in 100 μL of the FCM buffer with 5μL of the secondary Antibody (PE-anti human IgG Fc Recombinant, Biolegend, Catalogue 366903) and incubated for 30 minutes at 4°C. ...
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bioRxiv - Microbiology 2020Quote: ... 5 × 105 cells/mL of CD14+ enriched cells were stimulated using recombinant human (rh) GM-CSF and rhIL-4 (BioLegend, USA) at a concentration of each 500 IU/mL in RPMI1640 media supplemented with 10% FCS at 37°C in 5% CO2 ...
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bioRxiv - Immunology 2020Quote: ... once a week every week with IL-15 Receptor alpha Fc Chimera (R&D; 2.5μg/mouse) and recombinant human IL-15 (Biolegend, 5 μg/mouse). For immune blockade of PD-1 ...
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bioRxiv - Genomics 2022Quote: ... 0.2×106 naïve CD4+ T cells were cultured in presence of 10 ng/mL of recombinant IL-12 (BioLegend, Cat#577002), 1 ng/mL of recombinant IL-2 (BioLegend ...
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bioRxiv - Genetics 2023Quote: ... Recombinant Anti-AKT1 + AKT2 + AKT3 (phospho S472 + S473 + S474, Purified anti-Tubulin β-3 (TUBB3) Antibody 1:1000 (Biolegend, # PRB-435P), anti-GAPDH antibody 1:1000 (Sigma Aldrich ...
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bioRxiv - Cancer Biology 2023Quote: For inflammatory activation colon fibroblasts were plated in 6-wells and after 24 hours treated with 1 ng/ml murine recombinant IL-1α (Biolegend, 575002) in PBS for the indicated time periods ...
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bioRxiv - Immunology 2023Quote: ... For the memory B cell EliSpot, PBMC were stimulated with 1 µg/ml R848 (SigmaAldrich, SML0196) and 10 ng/ml recombinant human IL-2 (Biolegend, 791904) for 5 days before harvesting and plating of 50-100.000 cells/well ...
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bioRxiv - Cell Biology 2023Quote: ... breast cancer cells and myeloma cells were treated with recombinant hTRAIL (B294007, Biolegend, 1 to 300 ng/ml for different cells) for 3 h in the presence or absence of either HL-III (5 milliunits/ml) ...
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bioRxiv - Microbiology 2020Quote: ... The blot was then washed 3 times in PBS-T and probed with goat anti-rat HRP (Biolegend) and goat anti-mouse HRP (Biolegend ...
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bioRxiv - Immunology 2019Quote: ... C57BL/6 or BALB/c mice were treated with 100μg in 200μl PBS of anti-CD20 mAb (5D2, Genentech) or isotype control (Rat IgG2a, Biolegend) i.v ...
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bioRxiv - Immunology 2019Quote: ... C57BL/6 mice were treated with 100μg in 200μl PBS of anti-CD20 mAb (5D2, Genentech) or isotype control (Rat IgG2a, Biolegend) i.v ...
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bioRxiv - Immunology 2021Quote: ... Germinal center B cells were labeled using rat anti-GL7 antibody (FITC; BioLegend, catalog no. 144604, 1:250). Tfh cells were labeled using anti-CD4 antibody (BioLegend ...
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bioRxiv - Molecular Biology 2022Quote: ... Alexa Fluor® 647-conjugated rat anti-mouse CD104 (integrin β4) antibody (1:100, BioLegend, clone 346-11A), and FITC-conjugated rat anti-mouse Ly6d (1:100 ...
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bioRxiv - Molecular Biology 2022Quote: ... and were stained with APC/Cy7-conjugated rat anti-mouse CD45 antibody (1:200, BioLegend, clone 30-F11), Alexa Fluor® 488-conjugated or PE/Cy7-conjugated rat anti-mouse CD326 (EpCAM ...
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bioRxiv - Cell Biology 2019Quote: ... F4/80+ cells were then isolated by positive selection using a rat anti-F4/80 antibody (Biolegend, 123120) and sheep anti-rat Dynabeads (ThermoFisher Scientific ...
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bioRxiv - Cancer Biology 2022Quote: ... 3× before being incubated with APC-conjugated rat anti-CD83 antibody (1.25 μL/10×106 cells; Biolegend, 121510) for 30 minutes on ice ...
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bioRxiv - Immunology 2019Quote: ... Sections were washed three times with PBS and a secondary anti-rat IgG AlexaFluor488 antibody (BioLegend, London, UK) was used for detection ...
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bioRxiv - Immunology 2020Quote: ... The following antibodies were used: Alexa Fluor 700 Rat anti-mouse CD3 (17A2, BioLegend, San Diego, CA, USA), Pacific Blue Rat anti-mouse CD4 (GK1.5 ...
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bioRxiv - Bioengineering 2022Quote: ... and then 1:200 dilution of Alexa Fluor 488 mouse anti-rat CD45 (BioLegend, San Diego, CA, USA), PE mouse anti-rat CD31 (BD biosciences ...
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bioRxiv - Immunology 2022Quote: ... The cells were then incubated for 10 minutes with purified rat anti-mouse CD16/CD32 (Biolegend, 93 clone)(only for mouse cells ...
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bioRxiv - Microbiology 2023Quote: ... Sections were washed in PBS and surface antibodies were detected with anti-Rat AlexaFluor-488 secondary antibody (BioLegend). After surface staining ...