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Citations for BioLegend :
751 - 800 of 4666 citations for Recombinant Mouse CD19 Antigen His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2024Quote: ... followed by the staining with anti-His-APC (1:20, clone J095G46, BioLegend cat# 362605) with Fc block (1:20 ...
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bioRxiv - Microbiology 2020Quote: ... Primary detection of TRX-tagged YpTop1 was with primary murine monoclonal anti-TRX (BioLegend #658902, 1μg/ml), ...
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bioRxiv - Synthetic Biology 2020Quote: HA-tagged Gal4 TAs were stained with anti-HA.11 directly conjugated to Alexa Fluor 594 (BioLegend catalogue #901511 ...
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bioRxiv - Microbiology 2020Quote: ... lymph node and lung cells were stained with antibodies against multiple surface antigens: anti-CD45 (2µg/ml, 30-F11; Biolegend), anti-SiglecF (1µg/ml ...
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bioRxiv - Immunology 2020Quote: ... Antigen experienced CAR T cells were stained for CD69 expression using APC-conjugated anti-human CD69 antibody (Biolegend #310910). Flow cytometric data was acquired using a BD LSRFortessa with BD FACSDiva software ...
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bioRxiv - Cell Biology 2020Quote: ... Stem and progenitor cells were isolated using fluorescently labeled or biotinylated antibodies for the following antigens: cKit (2B8, Biolegend), Sca1 (D7 ...
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bioRxiv - Immunology 2022Quote: ... and extracellular antigens (1:200 CD3 BV650, clone 145-2c11, BD 564378; 1:200 NK1.1 PerCP-Cy5.5, clone PK136, BioLegend 10727) for 30 mins in PBS ...
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bioRxiv - Bioengineering 2023Quote: ... For surface antigen staining 20 min incubation with the Alexa Fluor 488 anti-human CD29 (Biolegend, clone TS2/16), PE anti-human CD184[CXCR4] (Biolegend ...
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bioRxiv - Immunology 2023Quote: ... Intracellular cytokine staining was performed according to manufacturer’s instructions for staining intracellular antigens (eBioscience) or using the True-Nuclear Transcription Factor Buffer Set (BioLegend). Flow cytometric analysis was performed using a FACS FORTESSA and FlowJo softwares (Tree Star ...
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bioRxiv - Immunology 2024Quote: ... PBMCs were thawed and staining for surface antigens was performed following Fc receptor blockade with Human TruStain FcX (BioLegend). Central/effector memory CD4+ T cells were defined as live/CD16-/CD14-/CD123-/CD56-/CD11c-/CD19-/CD4+ > excluding CD25hiCD127lo > CD45RA-/CD27+ ...
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bioRxiv - Systems Biology 2023Quote: ... The resulting single cells suspension was used to detect leukocyte populations (CD45 BV421 [clone: 104; Biolegend], CD4 PerCPeFluor710 [RM4-5; eBioscience], CD8a APC-Cy7 [53-6.7; Biolegend], CD19 PE-Cy7 [6D5; Biolegend]) and CD4+ Foxp3+ Treg (CD45 BV421 ...
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bioRxiv - Immunology 2020Quote: ... and were cultured with 50 ng/ml recombinant human GM-CSF (BioLegend #572904) and 50 ng/ml recombinant human M-CSF (BioLegend #574806 ...
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bioRxiv - Bioengineering 2021Quote: ... and 20 ng/mL recombinant human IL-3 (BioLegend; San Diego, CA, USA) to determine cellular distribution ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells were then treated with 100 ng/ml of recombinant human IFNγ (Biolegend) for 24h ...
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bioRxiv - Immunology 2021Quote: Mice were administered 0.75 µg of recombinant IL-33 (BioLegend, San Diego, CA) or PBS via intraperitoneal injection daily beginning at 3 days before amebic challenge up until 1 day before harvest.
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bioRxiv - Immunology 2022Quote: ... and 20ng/mL recombinant murine M-CSF (Shenandoah 200-08 or BioLegend 576404) in non-TC treated Petri dishes ...
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bioRxiv - Cancer Biology 2022Quote: ... “Rescue” wells were treated with 0.8 ug/mL recombinant human periostin (BioLegend 770506). Scratches were imaged at 0 hours ...
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bioRxiv - Immunology 2022Quote: ... Recombinant human SARS-CoV-2 spike S1 IgG lyophilized antibody (Clone AM009105, BioLegend) was used as a standard.
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bioRxiv - Immunology 2021Quote: ... RBD was then detected using an Alexa Fluor 488 conjugated anti-His antibody (clone J099B1, Biolegend). Cells were analyzed on a flow cytometry (BD).
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bioRxiv - Biochemistry 2022Quote: ... one slide was probed with Alexa Fluor® 647 anti-his antibody (1:250, BioLegend, 362611). Sera/plasma samples were single or serial diluted (three-fold dilutions from 1:90 ...
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bioRxiv - Cell Biology 2020Quote: ... Primary NK cells from lineage-depleted apheresis or NK92 cells were incubated on cells or cell-free matrix for 30 minutes in the presence of directly conjugated antibodies to surface antigens CD56 (1:50, clone HCD56, Biolegend) and CD29 (1:50 ...
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bioRxiv - Immunology 2021Quote: ... The resultant cell suspension was stained with the following antibodies directed against cell surface antigens for 30 minutes at 4°C: CD45::BV421 (H130, BioLegend) and HLA-DR::PE-Cy7 (L243 ...
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bioRxiv - Immunology 2020Quote: ... Enriched cells were then stained for the indicated cell surface antigens (Supplementary Table 3) in conjunction with TotalSeq-A oligonucleotide-conjugated antibodies (BioLegend) to allow for barcoding and pooling of different TEC subpopulations and subsequently sorted into 4 subpopulations ...
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bioRxiv - Immunology 2020Quote: ... The following surface antigens were detected by the indicated mAb clone: B220 (RA3-6B2-BV785 and RA3-6B2-BV421, BioLegend), CD21 (7EG-PerCP-Cy5.5 ...
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bioRxiv - Neuroscience 2021Quote: ... Except for immunohistochemistry for SOX-9 in which a β-gal staining was performed at 37°C for 3h followed by fixation with PFA 4% for 30 min at RT and incubation with unmasking antigen solution (927901; Biolegend). Sections were blocked and permeabilized in a permeabilization solution for 2h and incubated in goat polyclonal antibodies against SOX-9 (1:40 ...
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bioRxiv - Genomics 2023Quote: ... Fluorophore conjugated antibodies against the following antigens were used for input and day 3 cells (All Biolegend unless otherwise noted): panel 1 ...
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bioRxiv - Immunology 2023Quote: Biotinylated protein antigens were individually multimerized with fluorescently labeled streptavidin (BB515, BD Biosciences; BUV615, BD Biosciences; AF647, Biolegend; BV421, Biolegend) as described previously (25) ...
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bioRxiv - Immunology 2023Quote: Biotinylated protein antigens were individually multimerized with fluorescently labeled streptavidin (BB515, BD Biosciences; BUV615, BD Biosciences; AF647, Biolegend; BV421, Biolegend) as described previously (25) ...
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bioRxiv - Genomics 2024Quote: ... Antibodies were added to stain surface markers for 30 min at 4°C (CD3 PE, Life Technologies; CD8 FITC, Life Technologies; CD11b AF700, Life Technologies CD19 PerCP-Cy5.5, BioLegend; C33 BV605, BioLegend; CD56 BV711, BD). After 30min ...
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bioRxiv - Cancer Biology 2022Quote: ... The “rescue” wells were treated with 0.8 ug/mL recombinant human periostin (BioLegend 770506). After 24 hours ...
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bioRxiv - Biochemistry 2022Quote: ... and cells were cultured overnight with 0.5 μg/mL recombinant IL-2 (Biolegend 589106) in RPMI + 10% heat-inactivated FBS until use.
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bioRxiv - Developmental Biology 2022Quote: ... Plates were coated with recombinant human E-cadherin-Fc chimera (BioLegend #BLG-779904-25ug) to reduce colony formation and thereby limit the potential impact of paracrine and direct cell signaling in regulating gene expression of related cells 50 ...
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bioRxiv - Immunology 2023Quote: ... and 2 ng/ml human interleukin (IL)-2 recombinant (BioLegend, San Diego, CA, 589104).
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bioRxiv - Bioengineering 2023Quote: ... THP-1 cells were supplemented with 100 U/ml recombinant human IFN-γ (BioLegend) for 3 days to induce PD-L1 expression ...
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bioRxiv - Immunology 2023Quote: ... cells were incubated with 200 µg/mL recombinant Galectin-1 and Galectin-3 (Biolegend). Galectin binding was competitively inhibited by adding 50 mM lactose during the staining procedure.
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bioRxiv - Cell Biology 2023Quote: MAD-MB-453 cells were treated with recombinant hTRAIL (30 ng/ml, B294007, Biolegend) for 6 h ...
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bioRxiv - Cancer Biology 2024Quote: ... cells were treated with different combinations of 400 ng/mL human recombinant sFasL (Biolegend), 40 μM Fasudil HCl (SellekChem) ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... and a further 1 h incubation with anti-His HRP conjugate (652504; BioLegend, San Diego, CA, USA) diluted (1:20000 ...
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bioRxiv - Bioengineering 2023Quote: The antibodies used for flow cytometry were as follows: anti-His Tag APC (BioLegend, USA, Cat: #362605), anti-human IgG (G18-145 ...
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bioRxiv - Genomics 2020Quote: ... Cells were then washed once with full staining buffer (3% FBS in PBS with 2 mM EDTA) and surface antigens were stained for 30 min on ice using the following antibodies: gdTCR-BV421 (B1, Biolegend, 331218), CD45-Pacific Blue (HI30 ...
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bioRxiv - Immunology 2022Quote: ... Cells were then stained with rat polyclonal T antigen antibody (1:500) followed by an anti-rat secondary (Biolegend, 1:200). For measuring sialic acid binding dependence ...
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bioRxiv - Neuroscience 2021Quote: ... the cells were live-stained for two hours using a conjugated antibody targeted against an OVA257-264 peptide antigen (BioLegend, 141605). The cells were then fixed with 4% paraformaldehyde and imaged using the PerkinElmer Operetta CLS High-Content Analysis System ...
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bioRxiv - Immunology 2020Quote: ... YU2-gp140 fold-on, and hepatitis B surface antigen (HBs Ag) (Protein Specialists, hbs-875) conjugated to streptavidin Alexa Fluor 647 (Biolegend, 405237), streptavidin PE (BD biosciences ...
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bioRxiv - Biochemistry 2023Quote: ... FoxP3-transduced CD4+ T cells were stained with antibodies targeting the cell surface antigens CD25 (Biolegend, Cat#102022, 1:1000 dilution) and Thy1.1 (Biolegend ...
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bioRxiv - Cell Biology 2021Quote: ... purified mouse anti mouse/human CD207 (Biolegend, 144202), rabbit anti-mouse/human Ki67 (abcam ...
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bioRxiv - Cell Biology 2020Quote: ... mouse monoclonal against mouse β-III-tubulin (Biolegend); mouse monoclonal against HA (12CA5 ...
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bioRxiv - Immunology 2022Quote: Mouse cytokine (LEGENDplex™ Mouse Inflammation Panel, Biolegend) and chemokine (LEGENDplex™ Mouse Proinflammatory Chemokine Panel ...
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bioRxiv - Immunology 2023Quote: ... AF700-conjugated mouse anti-mouse CD45.2 (104; BioLegend), PE-Cy5-conjugated mouse anti-mouse CD45.1 (A20 ...
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bioRxiv - Cancer Biology 2020Quote: ... Cell sorting for GFP-positive CD298+MHC-I− PDX cells with fluorescently tagged antibodies for CD298 (Biolegend, cat. no. 341704) and MHC-I (eBioscience ...
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bioRxiv - Cell Biology 2022Quote: ... or P14 bone marrow cells from 2-4 mice per genotype were c-kit selected and stained with tagged Total-Seq B antibodies (Biolegend) to CD150 ...