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Citations for BioLegend :
751 - 800 of 1102 citations for Onion Yellow Dwarf Virus OYDV PCR Kits since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... Sera were assayed for cytokines using a 13-plex LEGENDplex Mouse Inflammation Panel kit (Biolegend, San Diego, CA) following the manufacturer’s instructions using an Accuri C6 flow cytometer (BD Bioscience ...
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bioRxiv - Immunology 2020Quote: ... Cell death was analysed 18h later by FITC Annexin V/PI Apoptosis Detection Kit with PI (640914; Biolegend).
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bioRxiv - Immunology 2021Quote: ... CD3+ cell enrichment was achieved by MojoSort negative selection using MojoSort mouse CD3+ T-cell Isolation Kit (BioLegend) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... the isolated B cells were incubated with 1 μl Zombie UV Fixable Viability kit (Biolegend, cat. no. 423107) per 1 ml cell suspension for 30 min on ice ...
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bioRxiv - Immunology 2022Quote: ... The concentration of CXCL10 in the media was determined using a Human CXCL10 ELISA MAX kit (BioLegend 439904).
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bioRxiv - Immunology 2022Quote: CD4+ T cells (naïve or total) were enriched with naïve or total CD4+ T cell negative enrichment kits (Stemcell, Biolegend) from the spleens of female ...
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bioRxiv - Systems Biology 2023Quote: ... Dead cells were stained with Fixable Viability Dye eFluor 780 (eBioscience) or Zombie Violet Fixable Viability Kit (BioLegend). Cells were stained with the following fluorochrome-conjugated anti-human Abs ...
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bioRxiv - Immunology 2022Quote: ... Naïve CD4 T cells were presorted with the MojoSort™ Mouse CD4 Naïve T cell Isolation kit (BioLegend) based on provided directions ...
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bioRxiv - Immunology 2022Quote: ... Twelve days post-treatment tumors were dissociated and subjected to CD45.1 positive selection (MojoSort mouse CD45.1 selection kit, Biolegend) per the manufacterer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: Cell suspensions were first stained for viability using Zombie NIR™ Fixable Viability Kit (BioLegend; San Diego, CA) for 20 min at room temperature in phosphate buffer solution (pH 7.2S ...
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bioRxiv - Immunology 2023Quote: TNFα and CCL5/RANTES were quantified in cell culture supernatants using mouse ELISA kits (BioLegend, R&D Systems) according to manufacturers’ instructions ...
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bioRxiv - Immunology 2024Quote: ... and IL-6 were measured by LEGENDplex flow-based 13-plex mouse inflammation panel kit from Biolegend (740446) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... Release of IL-6 and IL-8 were measured using commercial ELISA kits (Biolegend, San Diego, CA, USA) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... mast cells were stained using CD117 (c-Kit)-APC or APC/Cy7 and FcεRIα-PE (all from Biolegend) at 1:200 dilution for 20 min on ice ...
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bioRxiv - Immunology 2023Quote: ... Supernatant were collected and measured by ELISA kits according to the manufacturer’s instructions (catalog 438707, 440507, 431414; BioLegend). Absorbance was measured at 450 nm with a microplate reader (Molecular Devices).
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bioRxiv - Cell Biology 2023Quote: ... and T cells were isolated from the spleen using the MojoSortTM Mouse CD3 T cell Isolation Kit (Biolegend). Experiments where purified T cells were cultured for longer than 24 hours ...
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bioRxiv - Microbiology 2023Quote: ... Blood or tonsil CD4+ T cells were isolated using a human CD4+ T cell isolation kit (BioLegend #480010). Purified CD4+ T cells were used without stimulation or co-stimulated with plate-bound CD3 and soluble CD28 antibodies in the presence of 20 ng/ml IL-2 for three days ...
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bioRxiv - Immunology 2022Quote: ... naïve CD4 T cells were magnetically isolated from the spleen and LNs of Rag2-/- TCR7B8 Tg or TCR1A2 Tg mice on CD45.2/CD45.2 background using the MojoSort™ Mouse CD4 Naïve T cell Isolation kit (BioLegend). Then ...
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bioRxiv - Immunology 2023Quote: ... The amount of secreted IFN-γ was determined the LEGEND MAX™ Human IFN-γ ELISA Kit (BioLegend) according to the manufacturer’s instructions.
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bioRxiv - Molecular Biology 2023Quote: ... cells were subjected to a live/dead staining with the Zombie NIR™ Fixable Viability Kit (423105; BioLegend) and incubated in the dark for 10 min ...
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bioRxiv - Immunology 2023Quote: ... CD8+ T cells were isolated by negative selection using the MojoSort Mouse CD8+ T cell isolation kit (BioLegend) and cultivated as described above ...
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bioRxiv - Cell Biology 2023Quote: ... 0.5% BSA and 0.07% NaN3 in PBS) with additional Zombie Aqua™ (1:1,000 Fixable Viability Kit, BioLegend) to stain for vital cells ...
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bioRxiv - Immunology 2023Quote: ... Supernatants were collected after 24h and extracellular IL-2 was measured using the Biolegend ELISAMax Deluxe Kit (Biolegend), according to manufacturer instructions.
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bioRxiv - Genomics 2023Quote: ... CD4+ T-cells were then isolated using the human CD4+ T-cell MojoSort negative magnetic selection kit (Biolegend). Enriched CD4+ T-cells were then rested overnight in a 37°C 5% CO2 incubator in complete RPMI (RPMI1640 ...
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bioRxiv - Immunology 2023Quote: ... both human and murine single cell suspensions were first stained with a viability marker (Fixable Viability Kit, Biolegend) according to manufacturer’s instructions to exclude dead cells ...
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bioRxiv - Neuroscience 2023Quote: ... Cell suspensions were first stained for viability using Zombie NIR™ Fixable Viability Kit (BioLegend, San Diego, CA) for 20 min at room temperature in PBS combined with FcR blocking antibody (1 μg ...
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bioRxiv - Molecular Biology 2023Quote: Cells were trypsinized and stained with Annexin V and 7-AAD using APC Annexin V staining kit (BioLegend) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... untreated AML patients were determined by enzyme-linked immunosorbent assay (ELISA) using an IL21 Human ELISA kit (Biolegend), according to the manufacturer’s instructions.
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Fine tuning of CpG spatial distribution with DNA origami for improved therapeutic cancer vaccinationbioRxiv - Synthetic Biology 2023Quote: ... CD8 OT-I cells and CD4 OT-II were isolated by MojoSort kit purchased from BioLegend (#480035, #480033) according to the manufacturer protocol ...
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bioRxiv - Microbiology 2023Quote: Naïve B cells were isolated from spleens by immunomagnetic depletion using MojoSort Pan B cell Isolation Kit (Biolegend) and cultured in RPMI supplemented with L-glutamine ...
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bioRxiv - Bioengineering 2023Quote: Serum interferon alpha and beta were analyzed by ELISA (LEGEND MAX™ Mouse IFN-β ELISA Kit – BioLegend) (ELISA MAX™ Deluxe Set Mouse IFN-α1 – BioLegend).
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bioRxiv - Microbiology 2023Quote: ... resuspended in 100 μL Zombie NIR (1:1000 in PBS, Zombie NIR™ Fixable Viability Kit, Biolegend, #423106), transferred into a 96-well plate and incubated for 20 min at 4 °C in the dark ...
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bioRxiv - Bioengineering 2024Quote: ... cells were washed once more with PBS and stained with the Zombie VioletTM Fixable Viability Kit (Biolegend, USA) for 15 min following manufacturer’s instructions ...
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bioRxiv - Bioengineering 2024Quote: ... and cell pellets were washed with PBS before being treated with the Zombie NIRTM Fixable Viability Kit (Biolegend) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... cells were washed with PBS and stained for dead cells using Zombie UV Fixable Viability Kit (Biolegend, 423108) or Zombie NIR Fixable Viability Kit (Biolegend ...
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Downregulation of Let-7 miRNA promotes Tc17 differentiation and emphysema via de-repression of RORγtbioRxiv - Immunology 2024Quote: CD8+ naïve T cells were isolated from spleen using Mojosort Mouse CD8 Naïve T cell isolation Kit (Biolegend) and adjusted to a concentration of 1.0×106 cells/mL ...
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bioRxiv - Immunology 2024Quote: ... Single cell suspensions were stained with the Zombie NIR™ Fixable Viability KIT (780nm of excitation; Biolegend, #423106) at a 1/250 dilution in PBS (Biolegend ...
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bioRxiv - Microbiology 2024Quote: ... IL-6 and TNF-α ELISA kits at proper dilutions according to the manufacturer’s instructions (BioLegend, CA, USA). For the detection of cytokines in serum and peritoneal fluid ...
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bioRxiv - Cell Biology 2024Quote: ... The samples were subjected to staining using the Annexin V/Propidium Iodide (PI) apoptosis assay kit (BioLegend, USA) and subsequently analyzed using a BD FACSCalibur flow cytometer (USA) ...
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bioRxiv - Physiology 2024Quote: ... using the Pacific Blue Annexin V Apoptosis Detection Kit with 7-amino-actinomycin D (7-AAD) (BioLegend, #640926), according to the manufacturer’s instructions.
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bioRxiv - Microbiology 2024Quote: Cytokine concentrations in the supernatants were measured using the human antivirus response panel (13-plex) kit (LEGENDplex BioLegend). The kit was used according to the manufacturer’s manual with an additional fixing step ...
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bioRxiv - Microbiology 2024Quote: Single-cell lung suspensions were stained with the Zombie Aqua Fixable Viability kit (1:200 dilution, BioLegend, #423101) and treated with CD16/CD32 Fc block (1:100 dilution ...
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bioRxiv - Immunology 2024Quote: ... PBMC T cells were isolated from healthy donor using a MojoSort human CD3 T cell isolation kit (BioLegend), and FACS-sorted to yield the Siglec-7/9− population for subsequent coculture with macrophages ...
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bioRxiv - Immunology 2024Quote: ... CD8 T cells were isolated using the MojoSort Mouse CD8 T Cell Isolation Kit (BioLegend, cat. no. 480035). T cells were washed with fresh PBS 3 times through centrifugation ...
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bioRxiv - Immunology 2024Quote: ... Exclusion of dead cells and determination of cell viability was performed using Zombie UV Fixable Viability Kit (BioLegend). Surface antigen staining was performed in flow cytometry staining buffer (PBS supplemented with 1% bovine serum albumin ...
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bioRxiv - Immunology 2024Quote: ... Mouse CD4+CD62L+ naïve T cells were isolated using a MojoSort Mouse CD4 T Cell Isolation Kit (BioLegend) on an autoMACS Pro Separator (Miltenyi Biotec) ...
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bioRxiv - Immunology 2024Quote: ... OVA-specific CD4+ T cells were purified from the spleen and lymph nodes of OTII TCR transgenic mice using the MojoSortTM Mouse CD4 Naïve T cell isolation Kit according to the manufacturer’s instructions (Biolegend). Cells were then stained with the CellTraceTM Violet Cell proliferation kit (ThermoFisher ...
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bioRxiv - Immunology 2024Quote: ... Concentrations of cytokines were determined using the following kits according to the manufacturer’s instructions: IL-12p70 (Biolegend, 431704), TNF (Biolegend ...
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bioRxiv - Immunology 2024Quote: Foxp3-YFP+ cells were FACS sorted from the spleens of 3-week-old Gsk3a/bΔTreg and WTΔTreg mice following CD4+ T cell enrichment (MojoSort Mouse CD4+ T cell Isolation Kit, Biolegend) by negative selection ...
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bioRxiv - Immunology 2024Quote: ... and mouse CD4+ and CD8+ T cells were isolated using MojoSort Mouse CD4 T Cell Isolation Kit (BioLegend) and MojoSort Mouse CD8 T Cell Isolation Kit (BioLegend) ...