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Citations for BioLegend :
7601 - 7650 of 8552 citations for 5 Pyrimidinecarbonitrile 1 2R 2 3 dihydroxypropyl 1 2 3 4 tetrahydro 3 methyl 2 4 dioxo 9CI since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... magnetic activated cell sorting (MACS) was applied using the mouse IgM BG-1 antibody (BioLegend, San Diego, CA) coupled to α-mouse IgM microbeads (Miltenyi Biotec ...
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bioRxiv - Immunology 2023Quote: ... Cells were pelleted again and resuspended in 100 μl of 1:10 dilution of Human TruStain FcX (Biolegend) in MACS buffer ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were labeled with an antibody cocktail containing Anti-CD14-PE/Cy7 (Clone 63D3, 367111, BioLegend, 1:100), Anti-CD80-APC (Clone 2D10 ...
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bioRxiv - Bioengineering 2023Quote: ... the cells were washed with PBS containing 1% FBS and incubated with APC anti-His Tag Antibody (BioLegend) for 20 minutes on ice ...
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bioRxiv - Bioengineering 2023Quote: ... The following antibodies were mixed and added directly to the cell suspension: BV421 CD86 (1:200, Biolegend, 105031), PacBlue Ly6G (1:150 ...
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bioRxiv - Immunology 2023Quote: ... cells were washed with FACS buffer and stained with goat-anti-mouse IgG-Alexa Fluor647 (1:400, Biolegend) in FACS buffer for 20 minutes at 4°C ...
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bioRxiv - Immunology 2023Quote: ... Surface markers were subsequently stained on ice for 20 minutes: anti-mouse CD3-PE (1:200, 17A2, Biolegend), anti-mouse CD4-APC (1:200 ...
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bioRxiv - Genetics 2023Quote: ... proteins were transferred onto nitrocellulose membranes and probed with a 1:2,000 dilution of anti-RpoS antibody (Biolegend). For secondary antibody ...
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bioRxiv - Biochemistry 2023Quote: ... the membrane was blocked with 0.7% Na-caseinate pH 7.4-7.6 and 1% BSA and probed with the following primary antibodies: O-GlcNAc (clone RL2, BioLegend), OGT (ab96718 ...
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bioRxiv - Cell Biology 2023Quote: ... 0.5% BSA and 0.07% NaN3 in PBS) with additional Zombie Aqua™ (1:1,000 Fixable Viability Kit, BioLegend) to stain for vital cells ...
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bioRxiv - Developmental Biology 2023Quote: ... then for 1 hour at room temperature (RT) with biotinylated secondary antibodies and finally with fluorochrome-labeled (BioLegend) or peroxidase-labeled streptavidin (Beckman Coulter) ...
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bioRxiv - Immunology 2023Quote: ... stained with cell surface antibodies at 1:1200 (anti-CD8: PerCP-Cyanine5.5, eBioscience, # 45-0081-82, or Biolegend, #100734 ...
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bioRxiv - Cell Biology 2023Quote: ... neurons were incubated live with 10 µg/ml of β1-integrin antibody clone HM β1-1 (102202, Biolegend) for 15 min at 37 °C ...
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bioRxiv - Bioengineering 2022Quote: ... 25 μL of fluorescent counting beads (1 million/mL, 424902, Precision Count Beads, BioLegend, San Diego, California USA) were mixed with 100 μL of cell suspension for each condition ...
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bioRxiv - Neuroscience 2023Quote: ... and SMI-32 (using mouse Purified anti-Neurofilament H nonphosphorylated antibodies, 1:12000 dilution; Biolegend, San Diego, CA) immunohistochemistry (note that only sections adjacent to every 3rd Nissl-stained sections were processed for calbindin or SMI-32 ...
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bioRxiv - Developmental Biology 2023Quote: ... 1 million cells were incubated for 20 min at room temperature with Zombie viability dye (Biolegend ref 77143). Then cells were incubated in PBS + 10% FBS to block Fc receptors ...
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bioRxiv - Cancer Biology 2023Quote: ... The primary antibodies that were used in this study included rabbit anti- human CD271 (1:100, HPA004765, Biolegend), mice anti-human CXCL12 (1:50 ...
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bioRxiv - Immunology 2022Quote: ... The c-kit+ fraction was stained with antibodies against CD117 (c-kit APC, clone 2B8, Biolegend, 1/100), Sca-1 (Pacific Blue ...
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bioRxiv - Microbiology 2023Quote: ... gonorrhoeae (1 x 108 CFU) was incubated with 1.5 µl Tag-IT® Violet (TIV) (Biolegend, Catalog # 425101) in 100 µl 1X PBS + 5 mM MgSO4 ...
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bioRxiv - Immunology 2024Quote: ... sections were stained for 3h at room temperature with anti-CD3 PE-Dazzle594 (Biolegend, Cat: 100245, 1:50), anti-CD4 PE (Biolegend ...
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bioRxiv - Physiology 2024Quote: ... followed by 30-min incubation with the following diluted antibodies: BV605-anti-mouse CD45 (BioLegend #103139, 1:200), BV421-anti-mouse Ly6G (BioLegend #127627 ...
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bioRxiv - Immunology 2023Quote: ... and 1:20 dilution mouse anti-human CD27-PE (stock 50 ug/mL, BioLegend, San Diego, CA, USA), which has been reported as a marker for juvenile B cells ...
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bioRxiv - Immunology 2023Quote: ... anti-mouse CD279 (PD-1) (clone: RMP1-30, PE, Biolegend, cat# 109104; clone: RMP1-30, PE-Cy7, Biolegend, cat# 109110 ...
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bioRxiv - Biochemistry 2023Quote: ... using the following reagents: anti-HA (HA.11 Clone 16B12 Monoclonal antibody, BioLegend 901514, used at 1:1,000), Alexa Fluor 680 donkey anti-rabbit IgG(H+L ...
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bioRxiv - Bioengineering 2023Quote: ... were prepared in 1× Annexin V Binding Buffer (100 mM HEPES, 25 mM CaCl2, 1.4 M NaCl; BioLegend). To analyze cells ...
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bioRxiv - Cancer Biology 2023Quote: ... The single-cell suspensions were incubated in mouse Fc block (anti-CD16/32) antibody (Biolegend, 101335, 1:300) and then stained with the appropriate antibodies in FACS Buffer as described previously [33] ...
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bioRxiv - Immunology 2023Quote: ... kidneys were washed three times for 1 hr at room temperature in Ce3D™ Wash Buffer (427710, Biolegend), then cut in half and transferred to Ce3D™ Permeabilization/Blocking Buffer (48 hr ...
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bioRxiv - Cancer Biology 2023Quote: ... A far-red BST2 antibody was used to label BST2-expressing cells (diluted 1:40, BioLegend, cat. 348416). Cell viability was determined by Sytox Blue (diluted 1:1000 ...
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bioRxiv - Immunology 2023Quote: ... sorted TN and TEMRA cells were stimulated with plate-bound anti-human CD3 (OKT3; 1 μg/ml; BioLegend) and anti-human CD28 (CD28.2 ...
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bioRxiv - Developmental Biology 2023Quote: ... The grids were incubated with mouse anti-HA.11 epitope tag antibody (BioLegend, 901501, 1:1000, RRID: AB_2565006) for 30 min at RT ...
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bioRxiv - Neuroscience 2023Quote: ... Viability staining was performed using Zombie NIR for 10 minutes at room temperature (1:500 in PBS, BioLegend). Cells were pelleted and resuspended in anti-CD16/32 antibody (1:200 ...
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bioRxiv - Immunology 2023Quote: ... to block Fc receptors followed by staining with fluorochrome-conjugated anti–CD11b (1:200 dilution, BioLegend, no. 101226), anti–Ly6C (1:200 dilution ...
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bioRxiv - Immunology 2023Quote: ... approximately 1.0×106 PBMCs were firstly incubated with LIVE/Dead Zombie Aqua Dye (77143, BioLegend; 1 μL/well) for 15min at 4°C ...
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bioRxiv - Immunology 2023Quote: ... Cells were washed with PBS prior to staining with Zombie NIR Fixable live/dead dye (1:500) (Biolegend) for 20 min at 4°C ...
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bioRxiv - Biochemistry 2023Quote: ... The level of CTLA4 was measure by intracellular staining using anti-CTLA4 (Biolegend, Cat#106311, 1:1000 dilution) and the Transcription Factor Staining Buffer Set (eBioscience ...
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bioRxiv - Microbiology 2023Quote: ... resuspended in 100 μL Zombie NIR (1:1000 in PBS, Zombie NIR™ Fixable Viability Kit, Biolegend, #423106), transferred into a 96-well plate and incubated for 20 min at 4 °C in the dark ...
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bioRxiv - Neuroscience 2024Quote: ... Primary antibodies included a combination of the following: rat anti-CD4 antibody (Biolegend, UK, cat# 100505, 1:500), guinea pig anti-IBA1 antibody (Synaptic Systems ...
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bioRxiv - Developmental Biology 2024Quote: ... Cells were incubated for 30min with the following antibodies in 1:100 concentration: APC anti-mouse CD45 (Biolegend), APC anti-mouse Ter119 (Biolegend) ...
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bioRxiv - Immunology 2024Quote: ... Activated total PBMCs or isolated activated CD4+ and CD8+ T cells were treated with anti-PD-1 (BioLegend, Ultra LEAF purified human anti-PD-1 antibody ...
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bioRxiv - Neuroscience 2024Quote: ... Brain and spleen cells were stained with 1 µL from each FITC anti-mouse CD45 (Biolegend, Cat. 157214), PE anti-mouse CD11b (Biolegend ...
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bioRxiv - Neuroscience 2024Quote: ... and SMI-32 (using mouse Purified anti-Neurofilament H nonphosphorylated antibodies, 1:12000 dilution; Biolegend, San Diego, CA) immunohistochemistry.
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bioRxiv - Microbiology 2024Quote: ... for 10 min at RT and incubated with anti-mCherry developed in mouse (diluted 1:1000, Biolegend, 677702) and anti-TRAP (diluted 1:200 ...
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bioRxiv - Microbiology 2024Quote: ... Primary antibodies against the α-subunit of RNA polymerase were used at 1:5,000 (BioLegend, Lot No. B376827) with fluorophore conjugated antibodies against mice (Li-Cor ...
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bioRxiv - Immunology 2021Quote: ... and unique 5’ hashing antibody (TotalSeq™-C0251, C0254, C0256, and C0260, Biolegend) for an additional 30 minutes at 4C ...
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bioRxiv - Bioengineering 2020Quote: ... Jurkat cells were resuspended in 5 µM carboxyfluorescein diacetate succinimidyl ester (CFSE, Biolegend) in PBS for 20 min at room temperature in order to identify Jurkats specifically during cell sorting ...
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bioRxiv - Microbiology 2021Quote: ... APC mouse anti-mouse CD64 (Clone X54-5/7.1, Biolegend, San Diego, CA), APC-Cy7 rat anti-mouse CD11b (Clone M1/70 ...
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bioRxiv - Cancer Biology 2022Quote: ... AnnexinV/propidium iodide (PI) staining was performed using 5 µL AnnexinV (640905, Biolegend) per 500 µL HBSS ...
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bioRxiv - Cell Biology 2021Quote: ... and stained with E-Cadherin-AF647 (clone 67A4, 5 μg/ 100 μl, Biolegend) and CD44-PE (clone IM7 ...
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bioRxiv - Cancer Biology 2020Quote: ... Pelleted cells were re-suspend in 5 ml RBC lysis buffer (BioLegend 420301) for 10 min in RT and washed with 20 ml MACS buffer ...
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bioRxiv - Immunology 2021Quote: ... added with 5 µl of Human TruStain FcX™ Fc Blocking reagent (Biolegend). Cell suspensions were then incubated at 4°C for 10 mins ...