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Citations for BioLegend :
701 - 750 of 2680 citations for HSPB11 Human His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... 0.0032 μg biotinylated SARS-CoV-2 BA.1 protein (His & AVI Tag) (SinoBiological, 40592-V49H7-B) conjugated with PE-streptavidin or APC-streptavidin (TotalSeq-C0971 Streptavidin, BioLegend, 405271 and TotalSeq-C0972 Streptavidin ...
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bioRxiv - Immunology 2020Quote: Alexa fluor 488 or Alexa fluor 594 or FITC or Alexa fluor 648 labeled anti-His-tag (MBL or Biolegend),
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bioRxiv - Immunology 2023Quote: ... Soluble His6-tagged M6G at varying concentrations was incubated for 10 min with HRP-conjugated mouse anti-His antibody (BioLegend) diluted 1:2000 in 0.1% BSA/TBST ...
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bioRxiv - Immunology 2023Quote: ... Wells were then incubated with 100 μl HRP-conjugated mouse anti-His antibody (1:2000 dilution in 1% BSA/TBST, BioLegend).
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bioRxiv - Immunology 2023Quote: ... Purified proteins were then verified by SDS-PAGE as well as by capturing proteins on streptavidin beads and immunocytometry with anti-CD40L and anti-His tag antibodies (Biolegend), and compared to commercial soluble CD40L (ACROBiosystems).
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bioRxiv - Immunology 2021Quote: ... Monocyte isolation was determined with the following flow cytometry antibody panel after 10 min incubation with anti-human CD32 (StemCell – 18520): APC anti-human CD14 (Biolegend – 301808), PE-Cy7 anti-human CD3 (Biolegend – 344186) ...
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bioRxiv - Immunology 2021Quote: ... The following flow cytometry antibody panel was used to determine monocyte differentiation and M1 polarization after 10 min incubation with anti-human CD32: APC anti-human CD14 (Biolegend – 344186) BV421 anti-human CD80 (Biolegend – 305222) ...
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bioRxiv - Bioengineering 2024Quote: ... supernatant was removed for analysis with ELISA MAX Deluxe Set Human IL-2 and ELISA MAX Deluxe Set Human IFN-γ (BioLegend). Cytotoxicity at the various indicated timepoints was tested using a luciferase-based assay ...
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bioRxiv - Bioengineering 2024Quote: ... separate tubes were designated for FITC anti-human CD80 Antibody and APC/Cyanine7 anti-human CD206 (MMR) Antibody (BioLegend Europe B.V.) staining with an incubation time of 30 min ...
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bioRxiv - Immunology 2024Quote: ... cells were co-incubated with human CD16-expressing NK cells (NK92humCD16) for 4 hours at 37°C and the 421™ anti-human CD107a (Biolegend) antibody as a degranulation marker ...
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bioRxiv - Immunology 2024Quote: ... Alexa Fluor® 594 anti-mouse/human Ki-67 and Alexa Fluor® 647 anti-mouse/human CD324 (E-cadherin) antibodies (#801203, # 151213 and #147308, BioLegend). Each batch of anti-tubulin β3 antibody was titrated before use to minimize background staining ...
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bioRxiv - Immunology 2024Quote: ... B cells were isolated using the EasySep Human B Cell Isolation Kit (StemCell #17954) or the MojoSort Human Pan B Cell Isolation Kit (BioLegend #480082) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... PDX tumors were digested to a single cell suspension in Collagenase digest buffer and sorted for human cells using anti human HLA-ABC (Biolegend, 555555), DNA and RNA were extracted using an AllPrep Kit (Qiagen ...
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bioRxiv - Immunology 2023Quote: ... Skin immune cells were subsequently added to each fibroblasts-placed well and co-cultured in the presence of 20 ng/ml human IL-2 recombinant and 20 ng/ml human IL-15 recombinant (BioLegend, 570304) in 24 well plates (Ratio of 50:1 immune cell-to-fibroblast) ...
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bioRxiv - Cancer Biology 2023Quote: IGSF3-Fc or normal human IgG (250 ng) and APC-conjugated anti-human IgG-Fc antibody (BioLegend, San Diego, CA, USA) was added to 1 × 105 of MS1 cells suspended in 100 µL of FACS buffer (0.5% BSA/HBSS ...
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bioRxiv - Immunology 2024Quote: ... B cells were isolated by negative selection using the EasySep Human B Cell Isolation Kit (StemCell, #17954) or the MojoSort Human Pan B Cell Isolation Kit (BioLegend, # 480082) according to the manufacturer’s instructions.
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bioRxiv - Immunology 2019Quote: ... human T cells were directly enriched from PBMC by negative selection using MojoSort™ Human CD3 T Cell Isolation Kit (Biolegend, CA) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... approximately 2-10 million total human lung cells were resuspended in MACS buffer and blocked with Human TruStain FcX (Biolegend cat# 422032) for 15min at 4°C followed by HTII-280 antibody (Terrace Biotech ...
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bioRxiv - Cancer Biology 2019Quote: ... The TDEs were then washed and stained for intracellular cytokines using the PE/Cy7 anti-human IL-10 antibody (clone JES3-9D7) and Brilliant Violet 421™ anti-human LAP (TGF-β1) antibody (TW4-2F8) (BioLegend, CA). TDEs isolated from mice serum were stained with APC anti-human CD63 Antibody (Clone H5C6 ...
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bioRxiv - Bioengineering 2024Quote: ... The levels of cytokines IL-2 and IFN-γ were quantified using ELISA MAX Deluxe Set Human IL-2 and ELISA MAX Deluxe Set Human IFN-γ (BioLegend).
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bioRxiv - Microbiology 2021Quote: ... followed by washing and incubated with a solution of AlexaFluor® 647 conjugated anti-His antibody (BioLegend #652513, 10 µg/mL). After washing and drying ...
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bioRxiv - Immunology 2022Quote: ... 0.0032 μg SARS-CoV-2 WT biotinylated RBD protein (His & AVI Tag) conjugated with Brilliant Violet 605 Streptavidin and TotalSeq-C0973 Streptavidin (BioLegend, 405275) and TotalSeq-C0974 Streptavidin(BioLegend ...
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bioRxiv - Immunology 2022Quote: ... 0.0032 μg SARS-CoV-2 biotinylated RBD protein (His & AVI Tag) (SinoBiological, 40592-V27H-B) conjugated with APC-streptavidin (BioLegend, 405207), and 5 μL 7-AAD (Invitrogen ...
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bioRxiv - Immunology 2022Quote: ... 0.0032 μg SARS-CoV-1 biotinylated RBD protein (His & AVI Tag) (SinoBiological, 40634-V27H-B) conjugated with PE-streptavidin (BioLegend, 405204), 0.0032 μg SARS-CoV-2 biotinylated RBD protein (His & AVI Tag ...
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bioRxiv - Immunology 2023Quote: ... Cells were pelleted at 500xg for 5 minutes and then resuspended in 50uL of PBS+ 2% HI-FBS + 2mM EDTA in the presence of Fc receptor blocking antibody (BioLegend, 101302). Cells were labelled with antibodies as described below ...
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bioRxiv - Immunology 2023Quote: ... Cells were stained with Zombie-violet or Zombie-NIR in PBS at 1:2000 dilution for 5 minutes at room temperature prior to resuspending in PBS + 2% HI-FBS + 2mM EDTA in the presence of Fc receptor blocking antibody (BioLegend, 101302) for blocking ...
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bioRxiv - Cancer Biology 2021Quote: ... cells were incubated with human Fc receptor block (BioLegend) and stained with cell surface antibodies (Extended Data Table 4 ...
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bioRxiv - Cancer Biology 2021Quote: ... followed by incubation with human Fc receptor block (BioLegend) for 10 min at 4°C ...
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bioRxiv - Cancer Biology 2021Quote: ... cells were incubated with human Fc receptor block (BioLegend) and stained with cell surface antibodies (1:20 anti-CD3-Am Cyan [clone SK7 ...
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bioRxiv - Cell Biology 2020Quote: ... APC anti-human PVR/CD155 (Clone SKII.4, Biolegend), PE anti-human MICA (Clone 159227 ...
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bioRxiv - Cell Biology 2020Quote: ... APC anti-human Nectin-2/CD112 (Clone TX31, Biolegend), APC anti-human PVR/CD155 (Clone SKII.4 ...
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bioRxiv - Cell Biology 2020Quote: ... FITC anti-human ICAM-1/CD54 (Clone DX2, Biolegend), FITC anti-human Fas/CD95 (Clone DX2 ...
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bioRxiv - Immunology 2021Quote: ... BV421-conjugated mouse anti-human CD34 (Biolegend, CA, USA), PE-conjugated mouse anti-human CD270 (ThermoFisher Scientific ...
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bioRxiv - Microbiology 2021Quote: ... and Pacific Blue™ anti-human IgD (BioLegend, 348224). After 20 min ...
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bioRxiv - Developmental Biology 2021Quote: ... anti-human CD140a (PDGFRα) (Biolegend, clone 16A1; 1:50); anti-human CD309 (VEGFR2 ...
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bioRxiv - Developmental Biology 2021Quote: ... anti-human CD56 (NCAM) (Biolegend, clone HCD56; 1:100); anti-human CD184 (CXCR4 ...
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bioRxiv - Developmental Biology 2021Quote: ... anti-human CD184 (CXCR4) (Biolegend, clone 12G5; 1:100); anti-human CD117 (c-kit ...
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bioRxiv - Developmental Biology 2020Quote: ... anti-human CD140a (PDGFRα) (Biolegend, clone 16A1; 1:250); anti-human CD309 (KDR/VEG-FR2 ...
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bioRxiv - Immunology 2022Quote: PBMCs were firstly incubated with Human TruStain FcX (Biolegend) at 4 °C for 30 min and then stained with biotin-conjugated SARS-CoV-2 RBD protein (Sino Biological ...
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bioRxiv - Immunology 2021Quote: ... Fc receptors were blocked using human TruStain FcX (BioLegend), and cells were then subsequently stained for surface markers anti-CD11c-FITC (Bu15 ...
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bioRxiv - Immunology 2021Quote: ... mouse anti-human IgD-BV421 (Clone IA6-2, Biolegend) and mouse anti-human PD1-APC (Clone EH1202H7 ...
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bioRxiv - Immunology 2021Quote: ... and mouse anti-human PD1-APC (Clone EH1202H7, Biolegend) all diluted 1:100 ...
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bioRxiv - Immunology 2021Quote: ... mouse anti-human CD161-AF488 (Clone NKR-P1A, Biolegend), mouse anti-human IgD-BV421 (Clone IA6-2 ...
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bioRxiv - Immunology 2019Quote: ... The following anti-human antibodies were purchased from Biolegend, CD62L (DREG-56) ...
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bioRxiv - Cancer Biology 2019Quote: ... anti-human/mouse E-Cadherin-AlexaFluor 647 (147304; BioLegend) and DAPI for 15 minutes ...
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bioRxiv - Cancer Biology 2019Quote: ... and anti-human CD326 (EPCAM)-AlexaFluor 647 (324212; BioLegend), anti-human/mouse E-Cadherin-AlexaFluor 647 (147304 ...
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bioRxiv - Neuroscience 2019Quote: ... iMGLs were stimulated with human BMP9 (20ng/ml; Biolegend), Pam3csk (100 ng/ml ...
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bioRxiv - Cancer Biology 2021Quote: ... and consisted of anti-human CD3 APC-Cy7 (BioLegend), anti-human CD4 Brilliant Violet 786 (BioLegend) ...
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bioRxiv - Immunology 2021Quote: ... using both human and mouse Fc blocking reagents (BioLegend). Cellular suspensions were loaded on a Chromium Single-Cell Instrument (10x Genomics ...
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bioRxiv - Immunology 2021Quote: ... Human TruStain FcX Fc receptor blocking reagent (BioLegend #422302) was used at a 1:100 dilution in PBS with 2.5 g bovine serum albumin (Sigma Aldrich #A3059 ...