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Citations for BioLegend :
701 - 750 of 6828 citations for Fc Receptor Like Protein 3 FCRL3 Antibody since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... Proteins were detected by western blotting using an anti-RNAP β subunit antibody [clone 8RB13] (BioLegend), anti-σ70 antibody [clone 2G10] (BioLegend) ...
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bioRxiv - Immunology 2024Quote: ... rmIL-3 (Biolegend) was added to media (1 ng/ml) ...
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bioRxiv - Immunology 2023Quote: ... The CD16/CD32 receptors were then blocked by incubating with a 1/50 dilution of TruStain FcX™ (BioLegend) in PBS at room temperature for 15 min ...
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bioRxiv - Immunology 2024Quote: ... for the clone that binds to the high affinity IL-2 receptor (CD25): we incubated 0.5ugr mouse Il-2 (PepreoTech) to 5ugr of an IL-2 mAb (JES6-1A12, Biolegend) for 30 minutes on ice ...
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bioRxiv - Cancer Biology 2021Quote: ... Cell lines or expanded primary NK cells were stimulated with 3 ug/mL CD28 activating antibody (Biolegend, cat#302933) for 24 hours.
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bioRxiv - Genomics 2021Quote: ... for RNA library construction while modifications for CITE-seq ADT and cell hashing HTO library preparation were performed according to the TotalSeq™-A Antibodies and Cell Hashing with 10x Single Cell 3’ Reagent Kit v3 or v3.1 single index protocol (BioLegend). The modifications include the addition of ADT and HTO additive primers to a final concentration of 0.2 uM in the cDNA amplification reaction (step 2.2) ...
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bioRxiv - Immunology 2020Quote: ... These cells were stimulated with 10μg/ml of anti-CD3 (OKT-3) and anti-CD28 (28.2) antibodies (Ultra-LEAF™, Biolegend) in R10-50-15 for 48 hours ...
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bioRxiv - Bioengineering 2020Quote: ... dilutions of the serum were mixed with a constant 3 nM of an HRP conjugated anti-OVA antibody (BioLegend) and incubated for 2 h at room temperature ...
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bioRxiv - Immunology 2021Quote: ... and seeded in the antibody-coated flask at 3–5×105 cells/cm2 together with soluble 1 µg/mL anti-CD28 antibodies (clone 37.51; 102116, BioLegend). Cells were cultured for 48 hr at 37°C in a humidified 5% CO2 atmosphere ...
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bioRxiv - Synthetic Biology 2024Quote: ... 50,000 CAR-T cells were co-cultured with 100,000 target cells for 5h in the presence of CD107a antibody (Supp. Table 3) and 1x Brefeldin A (Biolegend). Following co-culture ...
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bioRxiv - Immunology 2024Quote: ... intestine and skin (n = 3, PCW 9.5) were stained with the following antibodies (clone, fluorophore, company): CD3 (UCHT1, FITC, Biolegend), CD7 (M-T701 ...
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bioRxiv - Immunology 2024Quote: Single cell suspensions from 3 B16-OVA WT tumours were labelled with TotalSeq(TM)-C0301 antibody (Biolegend, Cat. 155861), and 3 IFNγRKO tumours were labelled with TotalSeq(TM)-C0302 (Biolegend ...
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bioRxiv - Immunology 2024Quote: ... coupled with the protocol TotalSeq™-A Antibodies and Cell Hashing with 10x Single Cell 3’ Reagent Kit v3 or v3.1 (Single Index) from BioLegend.
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... For the HashTag library TotalSeq™-B0303 anti-mouse Hashtag 3 Antibody (Clone M1/42; 30-F11; 155835; BioLegend) was used to mark single cells of DMSO treated mice and TotalSeq™-B0304 anti-mouse Hashtag 4 Antibody (Clone M1/42 ...
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bioRxiv - Immunology 2022Quote: ... MEL624 was pre-treated with hIgG1-Fc (10ug/ml) (Biolegend, San Diego, CA) or Siglec-5-Fc chimeric protein (10ug/ml ...
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bioRxiv - Cancer Biology 2022Quote: ... resuspended in PBS/1% FCS and stained with anti-B220 (RA3-6B2, Biolegend), anti-Igµ (II/41 ...
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bioRxiv - Cell Biology 2022Quote: ... The single-cell suspension was resuspended in Fc-Block (Human TruStain FcX, BioLegend) and incubated with CD31 microbeads (Miltenyi Biosciences ...
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bioRxiv - Neuroscience 2022Quote: ... washed and incubated for 10 min with human Fc TruStain FcX (Biolegend #101320). Cells were stained for surface antigens ...
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bioRxiv - Immunology 2021Quote: ... and incubated with Fc block (1:100; Biolegend TruStain fcX, cat no. 101320) and fluorophore-conjugated antibodies at the appropriate concentrations (Table 3 ...
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bioRxiv - Immunology 2021Quote: ... cells were stained separately with Fc blocker (concentration 1:20) (TruStain FcX, Biolegend), rested for 15 minutes ...
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bioRxiv - Immunology 2021Quote: ... added with 5 µl of Human TruStain FcX™ Fc Blocking reagent (Biolegend). Cell suspensions were then incubated at 4°C for 10 mins ...
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bioRxiv - Immunology 2020Quote: ... cell suspensions were Fc-blocked using anti-CD16/32 mAb (BioLegend, clone 93) combined to murine serum 4% (Thermo Fischer) ...
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bioRxiv - Immunology 2021Quote: ... Cells were stained with 5 µL Fc-block (Human TruStain FcX, Biolegend 422302) and 1.5 µL of S167-180 HLA class II PE-conjugated tetramer for 30 minutes on ice ...
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bioRxiv - Immunology 2021Quote: ... resuspended in 50 µL FACS containing 5 µL human Fc-block (Biolegend, 422302), and blocked for 15 min at RT ...
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bioRxiv - Immunology 2024Quote: ... washed twice HBSS containing 2% FCS and incubated with Human Trustain Fcx (Biolegend) for 20 min on ice ...
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bioRxiv - Microbiology 2022Quote: ... cells were washed and stained with Fc block (Clone 93; Cat: 101320; BioLegend), CD45 (BUV395 ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... or 1:1000 (v/v) Alexafluor 647 conjugated Anti-Human IgG Fc (Biolegend) (for AT118-L Fc Fusion proteins ...
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bioRxiv - Immunology 2023Quote: ... All samples were then incubated with Fc block (5µg/mL CD16/CD32 (Biolegend) and 0.1% mouse serum (Sigma ...
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bioRxiv - Bioengineering 2022Quote: ... Cells were preincubated in FC Blocking solution (TruStain FcX™ PLUS, Biolegend, 156603) and Super Bright Staining Buffer (Invitrogen ...
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bioRxiv - Genomics 2022Quote: ... After the Fc blocking with 10 μl of FcX (BioLegend, cat# 422301/ 101319) for 10 minutes on ice ...
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bioRxiv - Cell Biology 2023Quote: ... 50,000 cells were used and blocked with Human TruStain FcX (Fc block, BioLegend) in FACS buffer (0.5% BSA (Sigma ...
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bioRxiv - Genomics 2023Quote: ... Cells were then resuspended in 100 mL/well of FC-block (Biolegend, 422302) (1:100 in Perm buffer ...
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bioRxiv - Immunology 2024Quote: ... Fc block was performed by incubating cells for 10min with TruStain FcX (Biolegend) diluted 1:25 in MACS buffer ...
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bioRxiv - Immunology 2024Quote: ... After incubation with Fc block (anti-CD16/32, 1:100, Clone S17011E, BioLegend), cells were stained with surface antibody mix (Supplementary Table S1) ...
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bioRxiv - Molecular Biology 2024Quote: ... 0.01M NaN3 solution and incubated with Human TruStain FcX™ (Fc blocker, Biolegend) for 15 min to block Fc receptors and reduce nonspecific binding ...
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bioRxiv - Immunology 2024Quote: ... and incubated with Fc block (TruStain FcX PLUS [anti-mouse CD16/32], BioLegend) for 10 minutes at 4°C ...
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bioRxiv - Immunology 2022Quote: ... and detection of indicated proteins was carried out using the following antibodies: Eos (W15169A; BioLegend, 1:500), pY-STAT5 (BD Biosciences ...
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bioRxiv - Cancer Biology 2024Quote: Oligonucleotides were conjugated to two antibodies against HGSOC cell surface proteins β2-Microglobulin (β2M) and CD298 (BioLegend) by iEDDA-click chemistry according to CITE-seq antibody-oligo conjugation protocol (https://cite-seq.com) ...
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bioRxiv - Immunology 2023Quote: ... Blocking antibodies specific for the following proteins were used: 2.5 μg/ml CD11b (M1/70, BioLegend, #101224), 2.5 μg/ml CD11c (N418 ...
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bioRxiv - Neuroscience 2023Quote: Primary antibodies to the following proteins were used at the indicated concentrations: ubiquitin (1:1000, P4G7, BioLegend; 1:200 Cell Signaling ...
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bioRxiv - Immunology 2023Quote: ... Intracellular proteins were stained using the following antibodies: anti-human T-bet PE (1:20, 4B10, BioLegend), and anti-human Ki-67 BUV395 (1:50 ...
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bioRxiv - Synthetic Biology 2021Quote: ... The knock-out of the T cell receptor (TCR) was assessed with the absence of signal after staining 1:200 with APC-labeled anti-CD3ε (Biolegend). Each CAR construct contained a Strep tag which allowed for a two-step staining to validate successful knock-in ...
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bioRxiv - Cell Biology 2020Quote: ... Primary antibodies targeting the various surface ligands and receptors as well as the corresponding control include: FITC anti-human HLA-A,B,C (Clone W6/32, Biolegend), FITC anti-human ICAM-1/CD54 (Clone DX2 ...
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bioRxiv - Immunology 2024Quote: ... Cells were then stimulated by cross-linking the receptors with mAb and streptavidin in a manner similar to phosphorylation kinetic assays (BioLegend). Cells were analyzed on the flow cytometer with UV fluorescence channel detectors by acquiring the first 30 seconds of baseline calcium levels and then measuring calcium influx levels upon the addition of streptavidin (500 ug/ml ...
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bioRxiv - Systems Biology 2021Quote: ... deeply anesthetized mice (5% isoflurane) were retro-orbitally injected with 3 μg of anti-CD45 antibody (PerCP, clone 30-F11, Biolegend) 3 min prior to euthanasia [46,81] ...
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bioRxiv - Immunology 2020Quote: ... Enriched cells were then stained for the indicated cell surface antigens (Supplementary Table 3) in conjunction with TotalSeq-A oligonucleotide-conjugated antibodies (BioLegend) to allow for barcoding and pooling of different TEC subpopulations and subsequently sorted into 4 subpopulations ...
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bioRxiv - Neuroscience 2021Quote: ... The sections were washed again 3 × 5 minutes in PBS and incubated overnight with rat anti-phospho-TDP primary antibody (1:100, 829901, BioLegend), in a dilution buffer (0.05% Tween ...
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bioRxiv - Immunology 2020Quote: ... Cocktail 2 was used to identify DE cells and contained 3 μl of anti-human CD5-APCeF780 antibody (clone L17F12, Biolegend), 2 μl of anti-human CD19-PECy7 antibody (clone HIB19 ...
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bioRxiv - Immunology 2020Quote: ... Cocktail 1 was used to identify CD3+CD14+ cells and contained 3 μl of anti-human CD3-AF700 antibody (clone UCHT1, Biolegend) and 1 μl of anti-human CD14-PE antibody (clone 61D3 ...
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bioRxiv - Genomics 2020Quote: Cell and nuclei labelling were performed according to an adapted Biolegend cell hashing protocol (TotalSeq™-A Antibodies and Cell Hashing with 10x Single Cell 3’ Reagent Kit v3 3.1 Protocol, Biolegend). Briefly ...