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Citations for BioLegend :
7251 - 7300 of 7947 citations for Rat Caprin 1 CAPRIN1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... The CD16/CD32 receptors were then blocked by incubating with a 1/50 dilution of TruStain FcX™ (BioLegend) in PBS at room temperature for 15 min ...
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bioRxiv - Immunology 2023Quote: ... Cells were then incubated with FITC, and PE-conjugated antibodies (1:100, IFNr or FoxP3, GZMB) purchased from Biolegend. Aqua LIVE/DEAD used for cell viability was purchased from Invitrogen ...
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bioRxiv - Immunology 2023Quote: ... The cells were then stained with Alexa Fluor® 700 anti-mouse CD206 (MMR) antibody (1:200; Biolegend, C068C2) for 30 min at room temperature ...
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bioRxiv - Cell Biology 2023Quote: ... for 20 min at 4°C in the dark with annexin V-FITC or - pacific blue (1:100, Biolegend), near-IR fixable viability dye (1:1000 ...
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bioRxiv - Bioengineering 2023Quote: ... The following antibodies were mixed together and added directly to the cell suspension: BV605 CD45 (1:500, Biolegend, 103139), AF700 CD11b (1:300 ...
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bioRxiv - Cancer Biology 2023Quote: ... were coated with 50 µL per well of Purified anti-human IFNγ Antibody (clone MD-1; #507502, BioLegend, USA) diluted 1:200 in 1X PBS (#5460-0023 ...
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bioRxiv - Neuroscience 2023Quote: ... Primary antibodies used for immunofluorescence on these tissues include: mouse anti-myelin basic protein (MBP) (1:1000; #808401 BioLegend), rabbit anti-MBP (1:200 ...
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bioRxiv - Immunology 2023Quote: ... 1ug/ml) and plate bound anti-CD3 (500 ug/ml) in the presence of Brefeldin A (1:1000, Biolegend) for 4 hours ...
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bioRxiv - Immunology 2023Quote: ... cells were stimulated with PMA/ionomycin with Brefeldin A (1:500 or equivalent to 1.3 μM ionomycin and 81 nM PMA, Cell Activation Cocktail, Biolegend) for 4 hours ...
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bioRxiv - Immunology 2023Quote: ... Primary and conjugated antibodies used in this study were: Armenian hamster anti-CD11c pacific blue (Biolegend, 1:100, #117321), goat anti-HIF-1α (R&D Systems ...
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bioRxiv - Systems Biology 2022Quote: ... Each tube was made up to 100μL with cell staining buffer and TotalSeq Hashtag (HTO 1-8) reagents (BioLegend) were added to each sample for 20min on ice ...
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bioRxiv - Cancer Biology 2022Quote: ... about 1-2e6 mononuclear cell suspension was stained with a panel of Total-seq B antibodies (1ug/each, Biolegend) and fluorochrome conjugated flow antibodies for CD45 (BD Biosciences) ...
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bioRxiv - Immunology 2023Quote: ... and stained for 45 min on ice in the dark with an APC anti-IFNγ (1:100 dilution, clone XMG1.2, Biolegend) intracellular marker ...
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bioRxiv - Cell Biology 2023Quote: ... Osaka University) were pre-incubated with anti-human-Fc secondary antibodies fused to APC (BioLegend, #410712, dilution 1:100) for 1 h in 1% BSA in PBS ...
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bioRxiv - Immunology 2023Quote: ... were coated with 1 μg/mL capture antibodies (IFNγ: clone R4-6A2, BD Pharmingen; or IL-17A: clone TC11-18H10.1, Biolegend) diluted in carbonate buffer ...
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bioRxiv - Immunology 2023Quote: ... The samples were incubated for 1 hour with fixation buffer from True-Nuclear™ Transcription Factor Buffer Set (Biolegend) and washed with permeabilization buffer from the same set ...
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bioRxiv - Cancer Biology 2023Quote: ... The resulting single cells were resuspended in PBS and stained with 1:1000 Zombie Violet™ dye (Biolegend, 423113) for 15 minutes to exclude dead cells ...
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bioRxiv - Synthetic Biology 2023Quote: ... resuspended in 50 µl LIVE/DEAD staining solution (PBS with 1:500 Zombie Violet Dye, Biolegend cat. no. 423113) and incubated for 20 min at 4 °C in the dark ...
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bioRxiv - Neuroscience 2023Quote: ... Samples were resuspended in 50 μl of ice-cold flow buffer (0.5% BSA, 1 mM EDTA, in 1x PBS, Sterile Filtered) containing antibodies targeting Cd11b (BV510, clone ICRF44, Biolegend), CD45 (APC/Cy7 ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were washed with PBS and intracellularly stained with PE anti-phospho-p38-MAPK (Thr180/Tyr182; 1:20; BioLegend) ab or PE mouse isotype control IgG1,κ (1:20 ...
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bioRxiv - Immunology 2023Quote: ... then fixed and permeabilized for intranuclear staining for 1 h at 25 °C with True Nuclear fixation buffer (BioLegend), washed twice with permeabilization/wash buffer ...
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bioRxiv - Developmental Biology 2023Quote: ... Primary antibody incubation was done overnight at 4οC with the following antibodies diluted in blocking buffer: PAX6 (1:300, 901301 Biolegend), P63 (1:100 ...
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bioRxiv - Neuroscience 2023Quote: Primary antibodies and dilutions used for immunofluorescence were: mouse Anti-Aβ (6E10 clone, 1:1,000, Biolegend, Cat# 803001, RRID:AB_2564653), Rabbit Anti-Aβ (6E10 clone ...
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bioRxiv - Neuroscience 2023Quote: ... and then incubated overnight at 4 °C with primary antibodies directed against amino acids 6-18 (Tau 12, 1:1,000 dilution, ref: 806501, Biolegend), 159–163 (Tau HT7 ...
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bioRxiv - Biochemistry 2023Quote: ... Naïve CD4+ T cells were then activated with anti-CD3 (Biolegend, Cat#100340, 1:500 dilution to 5μg/mL), anti-CD28 (Biolegend ...
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bioRxiv - Biochemistry 2023Quote: ... The purity was estimated to be >90% as measured by PE anti-CD4 (Biolegend, Cat#100408, 1:1000 dilution) staining and FACS analysis ...
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bioRxiv - Cell Biology 2023Quote: ... The slides were incubated with a primary rabbit anti-MMP2 antibody (cat# 87809, BioLegend, San Diego, CA; 1:100) overnight ...
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bioRxiv - Immunology 2023Quote: ... Antibody staining was performed on ice for 1 hour with APC/Cy7 anti-mouse CD45 (clone 30-F11; BioLegend), rat monoclonal FITC anti-mouse Ly6B.2 (clone 7/4 ...
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bioRxiv - Immunology 2023Quote: Single-cell suspensions from the tumors and draining lymph nodes were first stained with 1 μL Zombie Aqua (BioLegend) to exclude dead cells ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were resuspended in 1mL of PBS and live/dead dye used at 1:500 (Zombie NIR, BioLegend #423106). Cells were washed with 5mL of FACS buffer ...
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bioRxiv - Cancer Biology 2023Quote: ... Two million viable cells were resuspended in 100µl FACS buffer with 1 µl of Fc Block (anti-mouse Fc block CD16/32, Cat# 101302, Biolegend) for 20 minutes at 4°C ...
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bioRxiv - Cancer Biology 2023Quote: ... C57BL/6J mice bearing orthotopic KPC tumors were treated with 10 μg g−1 anti-TNF-α antibody (BioLegend) in combination with 100 mg/kg gemcitabine (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were washed with ice cold PBS and surface stain with primary anti-PD-L1 (1:50) antibody (BioLegend) on ice for 90 min ...
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bioRxiv - Cancer Biology 2023Quote: ... immunohistochemistry (IHC) and flow-cytometry (FC): Pacific Blue anti-mouse Ly-6A/E (Biolegend, Cat#108120, 1:50 FC), Sca-1/Ly6A/E (E13 161-7 ...
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bioRxiv - Cell Biology 2023Quote: ... Primary antibodies were dissolved in 5% BSA (Biofroxx, 4240GR005) and the dilutions were: Streptavidin-Cy3 (1:200, BioLegend, 405215), RL2 (1:200 ...
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bioRxiv - Bioengineering 2023Quote: ... Successful Differentiation was confirmed by flow cytometry using CD41 (APC, clone HIP8, Biolegend Cat. No. 303710, Dilution 1:200) and CD42b (PE ...
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bioRxiv - Genomics 2023Quote: ... Cells were stained on ice in the dark for 30 mins with CD34-APC (clone 581; Biolegend; 1:100), CD38-PE/Cy7 (clone HB7 ...
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The spindle protein CKAP2 regulates microtubule dynamics and ensures faithful chromosome segregationbioRxiv - Cell Biology 2023Quote: ... Primary incubation was done overnight at 4 °C with rabbit anti-CKAP2 1:2000 or mouse anti-GAPDH (BioLegend, 649201 ...
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bioRxiv - Immunology 2023Quote: ... were given by tail-vein intravenous (i.v.) injection in 200 μL of PBS with 1 μg/mL of anti-CD3 antibody (OKT3, Biolegend). Monthly post HPC transplant ...
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bioRxiv - Cancer Biology 2023Quote: ... FACS gates were set based on CD45 (Alexa Fluor® 594 anti-CD45, 1:20, Biolegend, #368520, clone 2D1), SYTOX Blue and DRAQ5 intensities to isolate live CD45+ and CD45-populations as shown in Supplementary Fig ...
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bioRxiv - Cell Biology 2024Quote: ... The sliced sections were incubated with an anti-HA monoclonal antibody (1:1000; BioLegend, San Diego, CA, USA, #901514), followed by anti-mouse Alex Fluor 488-conjugated immunoglobulin (1:100 ...
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bioRxiv - Cancer Biology 2024Quote: ... Cells were resuspended in cell staining buffer (PBS + 5 mM EDTA + 1% BSA + 1.6 mM NaOH + 0.01% sodium azide) and incubated in 1:100 diluted Zombie UV viability dye (Biolegend, 423107) or Zombie Violet viability dye (Biolegend ...
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bioRxiv - Cell Biology 2024Quote: The Chemokine concentrations in cell culture media were assessed using the 13-plex LEGENDplex Human Inflammation Panel 1 (Biolegend), according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: ... Retinas were then placed in primary antibody solution containing 1:1000 mouse anti-SMI-32 (BioLegend, Catalog #: 801701, RRID:AB_509997) in 3% normal donkey serum in 0.3% Triton PBS for 2-4 days at 4°C ...
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bioRxiv - Cancer Biology 2024Quote: MDA-MB-436 cells were resuspended in cell staining buffer (PBS + 5 mM EDTA + 1% BSA + 1.6 mM NaOH + 0.01% sodium azide) and incubated in 1:100 diluted Zombie Violet viability dye (Biolegend, 423113) for 5 minutes on ice ...
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bioRxiv - Cancer Biology 2024Quote: ... Samples were first incubated with True Stain fcXTM antibody (anti-mouse CD16/32, BioLegend, #101319, clone 93, 1:100) in PBS containing 0.5% BSA and 2 mM EDTA before staining with antibodies directly conjugated to fluorophores for flow cytometry ...
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bioRxiv - Neuroscience 2024Quote: ... The membrane was blocked with 5% milk in Tris-buffered saline with 0.1% Tween (TBST) and incubated overnight with primary antibodies: mouse anti-STAT1 (1:800, Cat# 603702, Biolegend), mouse anti-STAT1 phospho (Ser727 ...
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bioRxiv - Immunology 2024Quote: ... 500ul of fresh cell culture media for each respective cell type were combined with 10 µl of 0.5ug/µl purified anti-human ICAM-1 blocking antibody (Clone HA58, BioLegend), added to the wells ...
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bioRxiv - Molecular Biology 2024Quote: ... Membranes were blocked in 5% BSA in Tris-buffered saline/0.05% Tween-20 and proteins were detected by specific primary antibodies against MBP (#SMI 99, 1:1000; Biolegend), MOBP (bs-11184R ...
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bioRxiv - Developmental Biology 2024Quote: ... and with biotinylated antibodies against a panel of lineage markers (CD19, CD11b, CD11c, NK.1.1, Ter119, CD3ε, Gr-1 and B220 (BioLegend)) ...