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Citations for BioLegend :
7151 - 7200 of 10000+ citations for Rabbit Anti Human IgG Fc Biotin since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... and then incubated for 1 hr with either anti-RpoS or anti-RpoD antibodies (BioLegend, San Diego, CA, USA) in PBS-T containing 0.3% nonfat milk or ECL PrimeTM blocking agent ...
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bioRxiv - Immunology 2023Quote: ... Immunostaining was performed using the following primary antibodies: rat anti-mouse CD8α (clone 53-6.7, eBioscience) and Armenian hamster anti-mouse CD11c (clone N418, BioLegend). CD8 primary staining signal was further amplified using an anti-rat IgG secondary antibody conjugated with Alexa Fluor (AF ...
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bioRxiv - Cancer Biology 2023Quote: ... BV650 anti-CD4 (clone RM4-5, 1:600), AlexaFluor700 anti-CD8 (clone 53-6.7, 1:800) were purchased from BioLegend. e506 Fixable Viability Dye (1:1000) ...
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bioRxiv - Biochemistry 2023Quote: ... monoclonal mouse anti-HA 11 epitope (catalogue number: 901502) and mouse anti-Tubulinβ3 (catalogue number: 657402) were from BioLegend. Polyclonal rabbit anti-IKKα/β (H-470) ...
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bioRxiv - Immunology 2023Quote: ... Activation consisted of 5μg/mL plate-bound anti-mouse CD3ε antibody (100340) and 5μg/mL soluble anti-mouse CD28 antibody (102116) purchased from BioLegend, USA ...
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bioRxiv - Immunology 2024Quote: ... and the endothelial cells were separated using Dynabeads coupled to anti-CD31 antibody and anti-ICAM2 antibody (Biolegend, USA) according to the manufacturer’s instructions (Thermo fisher scientific ...
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bioRxiv - Cell Biology 2020Quote: ... containing 1% BSA and incubated with rabbit monoclonal CTR1 antibody (ab129067) or an isotype control antibody (Biolegend, clone Poly29108) for 1 hour on ice ...
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bioRxiv - Developmental Biology 2021Quote: ... Pacific Blue anti-mouse CD31 (rat, clone 390), anti-mouse CD45 (rat, clone 30-F11), anti-mouse Ter119 (rat, clone TER-119) were from BioLegend; APC anti-mouse CD326 or EpCAM (rat ...
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bioRxiv - Molecular Biology 2020Quote: ... and c-Kit on mast cells were analyzed by Flow cytometry using fluorochrome labelled antibodies (PE-CY7–conjugated anti-FcεRIα, MAR-1 Biolegend #134317; allophycocyanin-CY7–conjugated anti–c-Kit, 2B8, Biolegend, #105825). The YFP+ cells were FACS-sorted and deletion of the Gata2 gene was determined by PCR to be greater than 99%.
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bioRxiv - Immunology 2019Quote: ... mice were injected intraperitoneally with 100μg anti-TNFα (Ultra-LEAF™ Purified anti-mouse TNFα Antibody, clone MP6-XT22 Biolegend) or 200μg intraperitoneally (ip ...
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bioRxiv - Immunology 2019Quote: ... the membrane expression of BTLA and HVEM were assessed using respectively an APC conjugated anti-BTLA and a PE conjugated anti-HVEM (Biolegend). Cells were analyzed on a LSRII (BD Biosciences ...
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bioRxiv - Immunology 2021Quote: ... Undifferentiated donor 2395 iPSC were stained with anti-SSEA-4-APC and anti-Oct-4-PE (BioLegend, San Diego, CA) using the Fixation/Permeabilization Solution kit from BD Biosciences (San Jose ...
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bioRxiv - Bioengineering 2021Quote: ... Bcl-2 anti-apoptotic protein expression was measured by intracellular staining of BMMs with PE-Cy7 anti-Bcl-2 antibody (Biolegend). AlexaFluor488 anti-Bcl-2 and BV711 anti-CD11b antibodies (Biolegend ...
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bioRxiv - Bioengineering 2020Quote: ... Staining buffer used was a 1:1000 dilution of stock antibody solution (APC anti-mouse CD45; Alexa Fluor 488 anti-mouse Ly6G, Biolegend) into FACS buffer ...
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bioRxiv - Bioengineering 2020Quote: ... cells were first incubated in a cocktail of PE anti-mouse CD169 and PE anti-mouse F4/80 antibodies (BioLegend). After washing ...
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bioRxiv - Immunology 2021Quote: Staining for tumor-associated neutrophils was performed in processed tumor/biopsies samples with anti-CD45-PercP and anti-CD15-PE (Biolegend) for 15 min ...
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bioRxiv - Cancer Biology 2020Quote: ... and 200 μl of primary antibody (1:200 anti-αSMA antibody, eBioscience, clone 1A4, efluor 570; 1:200 anti-CD31 antibody, Biolegend, clone MEC13.3 ...
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bioRxiv - Cell Biology 2021Quote: ... dilution 1/250), anti-mCD71 mAb (RI7217, dilution 1/250) and anti-mTNFα mAb (MP6-XT722, dilution 1/250) from Biolegend; anti-mCD69 mAb (H1.2F3 ...
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bioRxiv - Immunology 2022Quote: ... The following antibodies were used to identify MKs: 1:100 anti-mouse CD41-FITC+ and anti-mouse CD42d-APC+ (BioLegend); and MKPs ...
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bioRxiv - Neuroscience 2022Quote: ... sections were incubated with primary antibodies (anti-GFAP, DAKO, z-0334, 1:1000; SMI-99 [anti-myelin basic protein] Biolegend; 808401 ...
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bioRxiv - Bioengineering 2022Quote: ... and then stained with the fluorescently labeled antibody that detects the corresponding cell surface receptor (anti-EGFR-BV421, Biolegend, Cat: 352911; anti-HER2-BV421, Biolegend, Cat ...
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bioRxiv - Immunology 2021Quote: ... fixed with 4% PFA for 15 minutes and subsequently stained for 30 minutes with anti-CD4-APC and anti-CD8-BV605 (Biolegend) in FACS buffer ...
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bioRxiv - Cancer Biology 2021Quote: ... cells were activated for 24h using 2 μg/ml of anti-Cd28 and anti-Cd3ε antibodies (102116 and 100340, BioLegend).
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bioRxiv - Microbiology 2020Quote: ... Cells were then stained for 30 minutes on ice with anti-CD11b-APC/Fire 750 and anti-F4/80-PE for macrophages or anti-CD11c-Alexa Fluor 647 for dendritic cells (BioLegend). Samples were washed twice and then run on Cytoflex (Beckman Coulter) ...
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bioRxiv - Cell Biology 2021Quote: ... for 10 min at 4°C and stained with fluorophore conjugated antibodies for 20 min at 4°C (anti-Pdpn PE-Cy7, Biolegend 127411; anti-Thy1 A647, Biolegend 105318 ...
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bioRxiv - Microbiology 2021Quote: ... the murine vasculature was labelled with 15 μg Dylight488-conjugated anti-mouse CD31 (LEAF purified anti-mouse CD31, clone 390, Biolegend, #102412 + DyLight488 Antibody Labelling Kit ...
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bioRxiv - Cancer Biology 2020Quote: ... Tumor sections were then incubated with fluorochrome-conjugated antibody (1:100, Alexa Fluor 488 anti-mouse F4/80, #123120, BioLegend; or PE anti-mouse CD40, #124610, BioLegend; or PE anti-iNOS ...
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bioRxiv - Immunology 2020Quote: ... and other epithelial cells markers (anti-mouse CD326 Brilliant Violet 605, 1:200 Biolegend 118227 and PerCP/Cy5.5 anti-mouse CD24, 1:200 Biolegend 101824) for 20 minutes at 4°C ...
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bioRxiv - Immunology 2020Quote: ... the cremaster muscles were stained by using primary antibodies including rabbit anti-mouse laminin α5 (antibody 405) or anti-mouse laminin α4 (antibody 377) and Brilliant Violet 411-conjugated rat anti-mouse MHC II (Biolegend); monocytes were identified by CX3CR1GFP expression ...
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bioRxiv - Immunology 2020Quote: ... CD4+ T cell purity was assessed by flow cytometry using anti-CD4 Alexa 700 (eBioscience) and anti-CD3 Brilliant Violet (BV) 785 (BioLegend) antibody staining ...
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bioRxiv - Immunology 2021Quote: ... REA159), Brilliant Violet 421 (BV421)-conjugated anti-pSTAT3 (pY705, 13A3-1) or BV421-conjugated anti-pAKT (pS473, M89-61) (Biolegend) Abs.
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bioRxiv - Immunology 2020Quote: ... Cells were then stained with 2 μg/mL of Alexa Fluor 647 labelled anti-CD5 antibody or anti-CD180 antibody (BioLegend) and 2 μg/mL of Alexa Fluor 488 labelled anti-IgM Fab fragment (Jackson ImmunoResearch ...
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bioRxiv - Immunology 2019Quote: ... Pre-enrichment for antigen presenting cells prior to sorting was done with biotinylated anti-CD3 (clone 145-2C11) and anti-CD19 (clone 6D5) both from Biolegend. Cell suspensions were incubated with antibodies for 10 minutes at room temperature at a dilution of 1:200 ...
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bioRxiv - Bioengineering 2020Quote: Purification of plant produced N and RBD (glycosylated and deglycosylated) variants were performed by anti-FLAG affinity chromatography using anti-DYKDDDDK affinity gel (cat. no. 651503, BioLegend) as described previously21 ...
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bioRxiv - Immunology 2021Quote: For FACS analysis the following antibodies were used: anti-CD11c APC and anti-I-A/I-E APC-Cy7 (Biolegend), anti-CD40 PeCy7 ...
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bioRxiv - Molecular Biology 2021Quote: ... CD4+ or CD8+ cells were stained with an APC-Cy7-labeled anti-CD4 and FITC-labeled anti-CD8 antibody (Biolegend), respectively ...
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bioRxiv - Immunology 2020Quote: ... Anti-CD69 (H1.2F3, PE/Cy7, B253212, 104512), Anti-CD3 (17A2, APC/Cyanine7, B283940, 100222) antibodies were purchased from BioLegend (Bxxxxxx) or BD Biosciences (xxxxxxx).
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bioRxiv - Immunology 2020Quote: ... buffer containing 1:100 dilution of target-specific antibodies (anti PD1:cat≠329905; Biolegend, and anti-PDL1: Cat≠329705; Biolegend) and incubated for 30 minutes at 4°C in the dark ...
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bioRxiv - Immunology 2020Quote: ... total thymocytes were activated with 1 μg/mL plate bound anti-CD3 and soluble 1 μg/mL anti-CD28 in the presence of monensin (BioLegend). After 4hrs ...
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bioRxiv - Immunology 2020Quote: ... PerCP-conjugated anti-CD11c (N418) and Pacific-Blue-conjugated anti-CD19 (6D5) antibodies were obtained from Biolegend (San Diego, USA). The APC-conjugated anti-CD154 antibody was obtained from Miltenyi Biotec ...
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bioRxiv - Immunology 2020Quote: ... Cells were then stained with anti-CD3-FITC (clone 145-2C11; 1:100; eBioscience) and anti-TCRδ (clone GL3; 1:100; Biolegend) for 30 min on ice ...
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bioRxiv - Immunology 2021Quote: ... Cells were then washed in 1x eBioscience Permeabilization Buffer twice and incubated with indicated intracellular antibodies including anti-TNF-PE (MAb1) and anti-IFN-γ-APC (4S.B3) from BioLegend at room temperature for 1 hour ...
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bioRxiv - Immunology 2021Quote: ... washed twice in buffer (PBS + 0.5% BSA) and stained with the anti-FLAG-phycoerythrin (PE) antibody (anti-DYKDDDDK Tag, BioLegend 637310) for confirmation of scFv expression and RSV-F protein conjugated with Alexa Fluor 647 (AF647 ...
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Convection and extracellular matrix binding control interstitial transport of extracellular vesiclesbioRxiv - Bioengineering 2022Quote: ... Fluorescently conjugated integrin antibodies (1:400 AF488 anti-integrin β1, clone-TS2/16, BioLegend; 1:200 PE anti-integrin β1, clone-TS2/16, BioLegend; 1:400 AF647 anti-integrin β1 ...
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bioRxiv - Cancer Biology 2022Quote: ... and activated using plate-bound anti-CD3 (1μg/ml; clone-145-2C11) and anti-CD28 (5μg/ml; clone-37.51) antibodies (Biolegend; San Diego, CA) for 4 days.
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bioRxiv - Microbiology 2022Quote: ... They were then washed once with the permeabilisation buffer of the staining buffer set and stained with anti-Tax-AF647 (clone LT-4) and either anti-Ki-67-PE (BioLegend) or anti-γ-H2AX-PE (clone 2F3 ...
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bioRxiv - Immunology 2022Quote: ... resuspended in 50 μl FACS-buffer containing the respective fluorochrome-coupled Abs (anti-TACI/CD267 PE, clone eBio8F10-3, eBioscience, anti CD138 PECy7, clone 281-2, Biolegend, anti CD19 BV510 ...
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bioRxiv - Bioengineering 2022Quote: ... PE dazzle 594 conjugated anti-CD235a (Clone: HI264; 349119) and BV605 conjugated anti-CD31 (Clone: WM59; 303121; all from Biolegend). EVs were then resuspended in 500 μL particle free PBS and analyzed using a BD FACSAria Flow cytometer (Becton Dickenson ...
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bioRxiv - Genomics 2024Quote: ... NTC and Rosa26 cell viability was assessed and surface expression of CD3 and B2M was compared to NTC and Rosa26 using fluorescence-labelled antibodies (anti-CD3 BV421, UCTH1 clone; anti-B2M FITC, 2M2 clone; both BioLegend) and flow cytometry (IntelliCyt iQue Screener Plus ...
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bioRxiv - Immunology 2024Quote: ... were cultured for 3 days in 96-well U bottom plates coated with 2 μg/mL anti-CD3 (eBioscience) and 1 μg/mL anti-CD28 (eBioscience) supplemented with 10ng/mL of recombinant mIL-2 (Biolegend) to allow Treg survival ...