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Citations for BioLegend :
651 - 700 of 967 citations for Recombinant Cynomolgus IL23R Fc tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... cells were then incubated for 10 minutes at 4°C in human Fc Blocking reagent (FcX, BioLegend). Cells were incubated at 4°C for a further 30 minutes with 0.5μg of TotalSeq-A (Biolegend ...
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bioRxiv - Genomics 2022Quote: ... and staining was performed in the presence of Fc block (eBioscience/ThermoFisher, True-Stain Monocyte Blocker (Biolegend), and Brilliant Stain Buffer (BD Bioscience) ...
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bioRxiv - Cancer Biology 2022Quote: ... was used as the primary antibody and rat anti-human IgG Fc antibody conjugated to APC (BioLegend) was used as the secondary antibody ...
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bioRxiv - Cancer Biology 2022Quote: ... Approximately 1×106 cells were aliquoted in 5 ml tubes and incubated with Fc Blocking antibody (Biolegend). Antibodies used for immune profiling are detailed in Supplementary Table 1 ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were stained with Zombie-NIR followed by Fc block with anti-mouse CD16/32 antibody (Biolegend). Cells were then stained for cell surface markers as described above ...
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bioRxiv - Cell Biology 2021Quote: ... they were blocked in 1% BSA in PBS and Fc blocker (unlabeled anti-CD16/32, Biolegend 101302) for 10 min at 4°C and stained with fluorophore conjugated antibodies for 20 min at 4°C (anti-Pdpn PE-Cy7 ...
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bioRxiv - Immunology 2021Quote: ... Following a wash, cells were incubated (RT, 10 min) with Rhesus Fc Receptor Binding Polyclonal Antibody (Biolegend). Desired staining antibodies were added directly to this mixture at indicated dilutions (Supplemental Table 27 ...
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bioRxiv - Neuroscience 2022Quote: ... in a protein low-binding microcentrifuge tube separately and incubated with 5 μL Fc blocking reagent (BioLegend) for 10 min at 4°C ...
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bioRxiv - Immunology 2022Quote: ... or plate-bound α-CD3ε (2 μg/mL) and plate-bound CD155-Fc (1 μg/mL; BioLegend) for four days ...
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bioRxiv - Developmental Biology 2022Quote: ... centrifuged and 500xg for 5 min and blocked with Fc block CD16/32 (Biolegend, #101302, 1:100), for 5-minutes on ice and stained with PECAM1-BV605 (Biolegend ...
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bioRxiv - Immunology 2019Quote: ... The following proteins and concentrations were used: ICAM-1-Fc (Cat. 553006, BioLegend, at 10 μg/mL), CD3 (clone 145-2C11 ...
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bioRxiv - Immunology 2019Quote: ... Fc-Block was performed (10µg/ml purified rat anti-mouse CD16/CD32, Biolegend TruStain FcX anti-mouse), after which cells were stained with Fixable Viability Staining e780 (1:1000 ...
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bioRxiv - Cancer Biology 2020Quote: ... 100,000–500,000 single cells of breast tumours were suspended in 100μl staining buffer (2% BSA, 0.01% Tween in PBS) before adding 10µl Fc-blocking reagent (FcX, BioLegend). and incubating during 10 min on ice ...
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bioRxiv - Immunology 2022Quote: ... and then incubated with Fc block TruStain FcX (CD16/32) to block nonspecific FcR binding (#422302, Biolegend). To detect MHC-I and MHC-II ...
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bioRxiv - Microbiology 2022Quote: ... 1×106 cells were plated and then incubated with Fc block (Biolegend; cat#101320; 1:100 dilution) and surface stained for 20 min at 4°C in the dark ...
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bioRxiv - Neuroscience 2022Quote: ... Fc receptors were then blocked with an anti-CD16/CD32 antibody (BioLegend, San Diego, CA, U.S.A., #553141). Cells were washed and then incubated with conjugated antibodies (FITC-CX3CR1 ...
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bioRxiv - Immunology 2023Quote: ... Nonspecific binding was blocked by incubating cells anti-mouse CD16/32 Fc block (Biolegend; cat. no. 101310). Sytox-Green viability dye (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2023Quote: ... Cells were resuspended in washing buffer (PBS 0.04% BSA) and incubated with mouse Fc blockers (FcX, Biolegend). Each sample was then incubated with either TotalSeq anti-mouse Hashtag Antibody 1 (Biolegend #155861 ...
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bioRxiv - Molecular Biology 2023Quote: ... 0.01% NaN3 in 1X PBS) and blocked with Fc receptor antibody specific for FcyR III/II (Biolegend TruStain FcX PLUS anti-mouse CD16/32 ...
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bioRxiv - Cancer Biology 2023Quote: ... Fc-block was performed by incubating cells in a dilution of Human TruStain FcX (#422302, BioLegend, USA) and mouse TruStain FcX (#101320 ...
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bioRxiv - Immunology 2023Quote: ... Cells were resuspended in semi-facs buffer in the presence of Fc receptor blocking antibody (BioLegend, 101302) and stained with antibodies at a 1:200 dilution with the myeloid or lymphoid antibody cocktails ...
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bioRxiv - Genomics 2023Quote: ... The PBMC pellet was resuspended and cells were stained with Human TruStain FcX Fc Blocking Reagent (BioLegend) for 10 min at 4°C and then with fixable viability dye DAPI (Merck) ...
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bioRxiv - Microbiology 2023Quote: ... Cells were incubated with 2 μg/mL of phycoerythrin (PE) anti-human IgG Fc (Clone M1310G05, BioLegend). Cells were washed once ...
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bioRxiv - Immunology 2023Quote: ... the cells were blocked in 1% BSA in PBS and Fc blocker (anti-CD16/32, Biolegend 101302) for 10 min at 4°C ...
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bioRxiv - Bioengineering 2023Quote: ... CAR-M cells was prepared and the Fc receptor was blocked with anti-moCD16/32 antibody (BioLegend) for 10 minutes at room temperature ...
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bioRxiv - Immunology 2024Quote: ... Cells that were used in CITE-seq experiments were blocked with TruStain Fc block (BioLegend; Cat. 422301) for 30 minutes ...
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bioRxiv - Bioengineering 2021Quote: ... such as the recombinant human matrix metalloproteinases 12 (MMP12, 1 μl, 20 ng/ml, Biolegend, U.S.A), MMP408 (the inhibitor of MMP12 ...
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bioRxiv - Bioengineering 2022Quote: ... were coated with 100ng per well of recombinant Spike RBD protein in 1×coating buffer (Biolegend) overnight at 4C° ...
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bioRxiv - Immunology 2020Quote: ... IL-2 immune complex (IL-2ic; mouse recombinant IL-2 + anti-IL-2 (JES6-1A12, BioLegend); molar ratio 2:1 ...
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bioRxiv - Immunology 2021Quote: ... in the presence of 1 μg/mL anti-CD28 and 1 ng/mL recombinant IL7 (BioLegend) for 48 hours ...
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bioRxiv - Immunology 2022Quote: ... Monocytes were cultured in 10 ng/mL recombinant human GM-CSF or M-CSF (both Biolegend) for 7 days at 37°C ...
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bioRxiv - Immunology 2022Quote: ... Monocytes were cultured in 10 ng/mL recombinant human GM-CSF or M-CSF (both Biolegend) for 7 days at 37°C ...
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bioRxiv - Immunology 2022Quote: ... Colon cells were either untreated or treated with 25 µg/ml recombinant mouse IFN-β1 (Biolegend.) Supernatant was collected for ELISA and cells were stained for flow.
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bioRxiv - Immunology 2023Quote: ... we cultured mouse T cells in mouse RP10 with recombinant mouse TGF-β1 (Biolegend Cat # 763104), rIL-12 ...
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bioRxiv - Immunology 2023Quote: ... DC2.4 cells were then treated with 5ng/ml Recombinant Mouse IFN-ψ (Biolegend, Cat. No:575302) for 24 hours ...
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bioRxiv - Cell Biology 2023Quote: ... Animals were then given retroorbital injections of 2.75×105 IU of recombinant mouse IFN-α (BioLegend), and the final 20 min of images with the same time intervals were collected ...
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bioRxiv - Immunology 2023Quote: ... in the presence of Th17 polarizing cocktails containing recombinant mouse IL-6 (10 ng/ml, Biolegend), mouse IL-1β (10 ng/ml ...
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bioRxiv - Biophysics 2023Quote: Antibodies uptake was determined by measuring the fluorescent intensity of a fluorescently-tagged antibody (Alexa Fluor® 647 anti-Tubulin-α, 0.5 mg/mL, BioLegend 627908) at a dilution ratio of 1:2000 ...
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bioRxiv - Immunology 2021Quote: Freshly thawed decidual immune cells (1-2 10e6 cells) were incubated with Fc blocker (Human TruStain FcX, Biolegend) in PBS with 1% BSA for 10 minutes at 4C and then surface stained with CD45-FITC (HI30 ...
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bioRxiv - Immunology 2022Quote: ... cells were pre-treated with 2.5 μg/mL anti-CD16/32 (Fc Block; 2.4G2; BioLegend, San Diego, CA) for 15 minutes before continuing with surface staining ...
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bioRxiv - Immunology 2021Quote: ... 1×105-1×106 cells were stained after Fc-blocking with TruStain FcX (BioLegend, San Diego, CA, USA) and incubation with Zombie Aqua fixable viability dye (BioLegend ...
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bioRxiv - Microbiology 2021Quote: ... Supernatants were aspirated and cells were then treated with Fc block (clone 93, Ref: 101302, BioLegend, 1:1000) at 4°C for 15 min ...
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bioRxiv - Immunology 2022Quote: ... Cells were washed twice after the primary stain and incubated with PE–anti-human Fc antibody (Biolegend, 410708) in MACS buffer for 20 min on ice ...
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bioRxiv - Immunology 2022Quote: ... Cells were then incubated in 2% FCS/PBS solution containing anti-mouse CD16/32 antibody (BioLegend clone 93) for 15 minutes and subsequently stained with a combination of fluorophore-conjugated primary antibodies against mouse CD45 (BioLegend clone 30-F11) ...
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bioRxiv - Physiology 2022Quote: ... 3 106 cells were then incubated with Fc receptor blocking solution (Human Trustain FcX, Biolegend, San Diego, USA) for 5 minutes at 4°C ...
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bioRxiv - Immunology 2022Quote: ... Cells were then stained with a mix of Fc Block (anti-mouse CD16/CD32 mAb; clone 2.4G2, BioLegend) and fluorophore-conjugated anti-mouse mAbs.
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bioRxiv - Immunology 2022Quote: ... cells were resuspended in FACS buffer (PBS + 4% FBS) supplemented with Fc block (clone 93; BioLegend Cat. 101320) and incubated at 4°C for 5 minutes before staining ...
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bioRxiv - Immunology 2019Quote: ... an Fc block step was performed by resuspending cells in 50μl of Fc block prepared in PBS/5%BSA (room temperature) using 5μl of Human Trustain FcX (Biolegend) per sample i.e ...
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bioRxiv - Cancer Biology 2019Quote: ... Fc receptors were blocked by incubation with 2.5 μg/mL anti-CD16/32 antibody (clone 93; 101320, BioLegend) in staining buffer (25 mM HEPES ...
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bioRxiv - Cancer Biology 2021Quote: ... 0.02% sodium azide) and resuspended in 25 µL 1:50 mouse FC block (TruStain FcX, BioLegend Cat# 101320), and left on ice for 5 minutes ...