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Citations for BioLegend :
651 - 700 of 9294 citations for 4 4 Fluoro 2 hydroxyphenyl methyl 2 6 bis 1 methylethyl 5 propyl 3 pyridinemethanol since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... for the clone that binds to the high affinity IL-2 receptor (CD25): we incubated 0.5ugr mouse Il-2 (PepreoTech) to 5ugr of an IL-2 mAb (JES6-1A12, Biolegend) for 30 minutes on ice ...
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bioRxiv - Immunology 2024Quote: ... The amount of IL-2 in the culture medium was measured using a mouse IL-2 ELISA kit (Biolegend).
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bioRxiv - Bioengineering 2024Quote: ... APC- CD81 (clone Eat-2; 2 µg/mL) detection antibodies or isotypic controls (all antibodies were purchased from BioLegend). Flow cytometry was performed on a MACSQuant 10 instrument (Miltenyi Biotech ...
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bioRxiv - Immunology 2021Quote: ... and seeded in the antibody-coated flask at 3–5×105 cells/cm2 together with soluble 1 µg/mL anti-CD28 antibodies (clone 37.51; 102116, BioLegend). Cells were cultured for 48 hr at 37°C in a humidified 5% CO2 atmosphere ...
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bioRxiv - Developmental Biology 2021Quote: ... E14 cells were collected from culture dishes and incubated for 30 min at 4 °C with CITE-seq cell hashing62 antibody Ab_CD15 (1:200) (Biolegend). XEN-eGFP were collected from culture plates and incubated for 30 min at 4 °C with CITE-seq cell hashing antibody Ab_CD140 (1:200 ...
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bioRxiv - Developmental Biology 2021Quote: ... XEN-eGFP were collected from culture plates and incubated for 30 min at 4 °C with CITE-seq cell hashing antibody Ab_CD140 (1:200) (Biolegend). In the gastruloid sample ...
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bioRxiv - Immunology 2021Quote: ... The depleted cell fraction was then stained for 20 min at 4 °C with the CD45-PerCPCy5.5 antibody (1:50) (Biolegend) and the EpCAM-FITC antibody (G8.8 clone ...
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bioRxiv - Immunology 2021Quote: ... The depleted cell fraction was then stained for 20 min at 4 °C with a cocktail of fluorophore-labeled antibodies CD45-PerCPCy5.5 (1:50) (Biolegend), Ly51-PE (1:800 ...
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bioRxiv - Neuroscience 2021Quote: ... the membranes were incubated overnight at 4°C with anti-Aβ primary antibody (6E10 Biolegend, SIG-39320, 1:1000) following incubation with goat anti-mouse secondary antibody (Thermo Scientific ...
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bioRxiv - Immunology 2021Quote: ... about 4 × 106 cells were stained with 1 μl mouse Fc-block (anti-CD16/32; BioLegend, San Diego, CA) in 50 μl FACS buffer (PBS containing 2% FBS ...
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bioRxiv - Neuroscience 2022Quote: ... and incubated at 4°C overnight with the following primary antibodies: mouse anti-C3 (1:200 dilution, 846302, Biolegend), rabbit anti-CD31 (1:200 dilution ...
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bioRxiv - Immunology 2024Quote: ... cells were harvested and stained for 30min at 4°C with anti-CD25 APC (Biolegend, Cat: 102012, 1:50), and ZombieUV (Biolegend ...
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bioRxiv - Immunology 2024Quote: ... Stained spleen or LN samples were fixed with 1-4% phosphate-buffered paraformaldehyde solution (FluoroFix or Fixation Buffer, Biolegend). To detect transcription factors and certain intracellular proteins ...
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bioRxiv - Immunology 2023Quote: ... Slides were then blocked and incubated overnight at 4°C with anti-Lysozyme antibody diluted 1:400 (Biolegend, #860001). Slides were washed and incubated with anti-rabbit Alexa Fluor 555 diluted 1:500 (Invitrogen ...
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bioRxiv - Cell Biology 2023Quote: ... for 20 min at 4°C in the dark with annexin V-FITC or - pacific blue (1:100, Biolegend), near-IR fixable viability dye (1:1000 ...
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The spindle protein CKAP2 regulates microtubule dynamics and ensures faithful chromosome segregationbioRxiv - Cell Biology 2023Quote: ... Primary incubation was done overnight at 4 °C with rabbit anti-CKAP2 1:2000 or mouse anti-GAPDH (BioLegend, 649201 ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells were stained for 30 min at +4°C with APC or PE-conjugated anti-CD133 antibodies (diluted at 1/200 or 1/400 respectively; Biolegend, San Diego, USA) or corresponding control Immunoglobulin G1 (IgG1 ...
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bioRxiv - Molecular Biology 2024Quote: ... according to manufacturer’s recommendations and stained for 1 hour at 4°C with the following antibodies: NGFR (345110; 1:600, BioLegend, San Diego, CA, USA), α-tubulin (NB100-69AF405 ...
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bioRxiv - Immunology 2021Quote: ... 18 μL of PHA (1 μg/μL stock) and 2 μL of Cell activation cocktail (Biolegend) are added to the tube ...
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bioRxiv - Cancer Biology 2022Quote: 1–2 × 106 NB4 treated cells were harvested and labelled with FITC-conjugated Annexin-V (Biolegend) for 15 min according to the manufacturer’s instruction ...
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bioRxiv - Immunology 2020Quote: ... for 30min followed by staining with αLFA1-Alexa Fluor647 (Clone: CBR LFA 1/2, BioLegend, 366311) for 30 minutes ...
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bioRxiv - Immunology 2022Quote: ... were coated with 1 µg/ml anti-CD3 and 2 µg/ml anti-CD28 antibodies (BioLegend) for 2 hours and seeded with 50.000 T cells/well ...
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bioRxiv - Immunology 2023Quote: ... BA.1 or BA.2 biotinylated RBD protein conjugated with TotalSeq™-C0971 Streptavidin (BioLegend, 405271) and TotalSeq™-C0972 Streptavidin (BioLegend ...
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bioRxiv - Immunology 2024Quote: ... Supplementary Table 1) and 1ul/staining volume of hashtag TotalSeqC antibody mastermix (Biolegend, Supplementary Table 2) was added to each sample and cells were incubated for 30 minutes at 4°C ...
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bioRxiv - Neuroscience 2023Quote: ... The blocking of nonspecific binding was induced in the cell suspensions via incubation for 10 min at 4°C with anti–CD16/CD32 antibody (cat # 101320, BioLegend, clone 93, 5 ng/μl). This was followed by staining for 30 min at 4°C with the appropriate antibodies ...
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bioRxiv - Immunology 2021Quote: ... plates were coated with anti-mouse IL-4 antibody (11B11, Biolegend) then blocked as above ...
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bioRxiv - Immunology 2022Quote: ... and 4 μL of anti-CD193-PerCP/Cy5.5 (clone 5E8, BioLegend), and incubated the sample for ~18 minutes on ice ...
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bioRxiv - Immunology 2022Quote: ... 20 ng/ml IL-4 + 20 ng/ml IL-13 (Biolegend) for polarization to M2 ...
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bioRxiv - Immunology 2020Quote: ... Bcl6 PE/Dazzle (clone 7D1, BioLegend, cat# 358510, dilution 4:100). Data was recorded on a FACSymphonyTM flow cytometer (BD Biosciences ...
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bioRxiv - Immunology 2020Quote: ... IL-4 (11B11) and CD124 (I015F8) mAbs were purchased from BioLegend. Anti-CD44 (IM-7) ...
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bioRxiv - Immunology 2020Quote: ... Plates were washed 4 times with washing buffer (BioLegend; Cat: 4211601) and then incubated for 1 h at RT with a 1:2000 dilution of secondary antibody of horse radish peroxidase (HRP)-conjugated rabbit anti-mouse IgM (μ chain) ...
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bioRxiv - Immunology 2020Quote: ... or 40 ng/mL IL-4 (Biolegend, San Diego, CA, USA) in duplicates respectively for 24 hours in X-VIVO 15 serum free medium ...
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bioRxiv - Immunology 2022Quote: ... and 10 μg/mL anti-IL-4 Ab (clone 11B11, BioLegend) were added to the culture media ...
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bioRxiv - Cancer Biology 2022Quote: ... we stained the cells with 4 μg/ml PE-streptavidin (BioLegend) at the 37 °C for 45 min ...
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bioRxiv - Immunology 2022Quote: Mouse splenocytes were stained with 4 μg/ml LAG3 BV785 (BioLegend) and 4 μg/ml CD49b PeCy7 (BioLegend ...
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bioRxiv - Immunology 2024Quote: ... anti-IL-4 (#500808) was from BioLegend (San Diego, CA, USA). Cells detached from culture plates were filtered through 70-μm cell strainers ...
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bioRxiv - Pathology 2023Quote: ... Neutrophils were maintained 4 hours in culture with Brefeldin A (Biolegend) to stop exocytosis.
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bioRxiv - Bioengineering 2023Quote: ... cells were fixed with 4% paraformaldehyde (Biolegend, San Diego, CA, USA) for 10 minutes and subsequently permeabilized with 0.1% Triton X100 (Merck KGaA ...
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bioRxiv - Cancer Biology 2023Quote: ... 0.1% Triton-X and 4 µg/mL DAPI (#422801, BioLegend, USA) for 1 h at RT and stained with two staining panels ...
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bioRxiv - Microbiology 2023Quote: ... PE/Cyanine7 anti-mouse IL-4 antibody (BioLegend, catalog no. 504118), and Brilliant Violet 711 anti-mouse IL-17A Antibody (BioLegend ...
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bioRxiv - Immunology 2023Quote: ... after stimulating cells for 4 hours with Brefeldin A (Biolegend #420601). Absolute cell counts were determined using counting beads (Biolegend #424902) ...
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bioRxiv - Immunology 2023Quote: ... after stimulating cells for 4 hours with Brefeldin A (Biolegend #420601). Absolute cell counts were determined using counting beads (Biolegend #424902) ...
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bioRxiv - Cell Biology 2023Quote: ... TotalSeq™-A0304 anti-mouse Hashtag 4 Antibody) (BioLegend, TotalseqA antibodies). The 4 biological replicates of each time point were stained with one of the 4 unique hash antibodies ...
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bioRxiv - Cancer Biology 2023Quote: ... FITC anti-mouse CD4 Antibody (BioLegend, catalog #116003, clone RM4-4), PerCP/Cyanine5.5 anti-mouse CD4 Antibody (BioLegend ...
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bioRxiv - Immunology 2024Quote: ... KO iv-: TotalSeq-A0304 anti-mouse Hashtag 4 (all from Biolegend). Equal number of cells from each population were pooled and then subjected to 10X single cell RNA seq library preparation targeting 1×104 total cells ...
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bioRxiv - Immunology 2021Quote: ... Supernatants were harvested 24 hours later and IL-2 concentrations were determined using serially diluted supernatants and the human IL-2 ELISA MAX Deluxe kit (BioLegend) adapted to 384-well plate format using one quarter of the recommended reaction volumes ...
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bioRxiv - Immunology 2021Quote: ... in culture medium supplemented with 0.5 µg/mL soluble anti-mouse CD28 (eBioscience) and 10 ng/mL (∼50 U/mL) recombinant murine Interleukin-2 (IL-2, BioLegend), and activated for up to 72h ...
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bioRxiv - Bioengineering 2021Quote: ... Bcl-2 anti-apoptotic protein expression was measured by intracellular staining of BMMs with PE-Cy7 anti-Bcl-2 antibody (Biolegend). AlexaFluor488 anti-Bcl-2 and BV711 anti-CD11b antibodies (Biolegend ...
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bioRxiv - Immunology 2022Quote: ... Frozen PBMC of post-CA patients were thawed and incubated in media (RPMI1640 + 2% FBS) with recombinant human Nectin-2 (Biolegend) or isotype control (ENZO ...
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bioRxiv - Immunology 2020Quote: ... Culture supernatants were removed and analyzed for IL-2 production as a correlate for T cell activation using an ELISA MAX mouse IL-2 set (BioLegend). IL-2 values measured in supernatants from the wells containing BMDCs pulsed with proteins for different time periods were transformed into µM peptide equivalents by interpolating in a standard curve generated from the values obtained from hybridomas co-cultured with BMDCs at indicated peptide concentrations.