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Citations for BioLegend :
6901 - 6950 of 7730 citations for Insulin ELISA Kit 1 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: ... and double knockdown cells were treated with the IC50 value of cisplatin and viability was assessed via Annexin V and propidium iodide staining by flow cytometry (Biolegend APC Annexin V Apoptosis Detection Kit).
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bioRxiv - Cancer Biology 2021Quote: ... Tissue for sequencing was incubated for 1 hour with 0.5 μM Sytox™ Blue solution (Biolegend, catalog no. 425305) to counterstain cell nuclei ...
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bioRxiv - Developmental Biology 2021Quote: ... 0.02% Tween-20 in PBS) and incubated with 1 µg of a unique TotalSeq-A nuclear hashing antibody (Biolegend) for 30 min at 4°C ...
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bioRxiv - Cell Biology 2019Quote: ... Fixed and blocked hMPCs were incubated overnight at 4°C with an anti-BrdU antibody (1:400 dilution, Biolegend) followed by PBS washes and a 60 min incubation with an Alexa-Fluor 488 conjugated anti-mouse secondary antibody (Invitrogen) ...
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bioRxiv - Cell Biology 2020Quote: ... containing 1% BSA and incubated with rabbit monoclonal CTR1 antibody (ab129067) or an isotype control antibody (Biolegend, clone Poly29108) for 1 hour on ice ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were stained for 20-30 minutes on ice with 1:200 anti-CD11b-BV711 (Clone M1/70; Biolegend) prior to analysis by flow cytometry ...
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bioRxiv - Cell Biology 2020Quote: ... Cell pellet was further resuspended in 150ul PBS+0.5%BSA and stained with PE anti-human CD56 antibody (1:100, Biolegend) for 20min in dark ...
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bioRxiv - Immunology 2021Quote: ... in PBS) for 30 minutes with 1:400 dilution of FITC Mouse anti-Human HLA-A2 (clone BB7, Biolegend), APC anti-human HLA-A,B,C (clone W6/32 ...
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bioRxiv - Immunology 2021Quote: ... in PBS with 1% BSA for 10 minutes at 4C and then surface stained with CD45-FITC (HI30, Biolegend) for 30 minutes in the dark ...
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bioRxiv - Developmental Biology 2021Quote: ... E14 cells were collected from culture dishes and incubated for 30 min at 4 °C with CITE-seq cell hashing62 antibody Ab_CD15 (1:200) (Biolegend). XEN-eGFP were collected from culture plates and incubated for 30 min at 4 °C with CITE-seq cell hashing antibody Ab_CD140 (1:200 ...
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bioRxiv - Immunology 2021Quote: ... then stained with the I-A/E-APC antibody (MHCII) (1:1,200) (eBioscience) and positively selected for I-A/E-positive cells using anti-APC Microbeads (Biolegend). The obtained final cell fraction consisted of total mTECs (CD45-EpCAM+Ly51-I-A/E+).
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bioRxiv - Immunology 2021Quote: ... The depleted cell fraction was then stained for 20 min at 4 °C with the CD45-PerCPCy5.5 antibody (1:50) (Biolegend) and the EpCAM-FITC antibody (G8.8 clone ...
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bioRxiv - Immunology 2021Quote: ... The depleted cell fraction was then stained for 20 min at 4 °C with a cocktail of fluorophore-labeled antibodies CD45-PerCPCy5.5 (1:50) (Biolegend), Ly51-PE (1:800 ...
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bioRxiv - Molecular Biology 2021Quote: ... the cells were incubated for 1 h on ice in PE/Cy7 anti-mouse CD45.2 antibody (clone:104, BioLegend) and PE-conjugated anti-mouse IL1a antibody (clone:ALF161 ...
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bioRxiv - Molecular Biology 2021Quote: CH12 cells were stimulated to undergo CSR to IgA by treatment with 1-5 μg/mL α CD40 (BioLegend), 5 ng/ml TGF (R&D Systems ...
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bioRxiv - Developmental Biology 2021Quote: ... 500 ng/ml rAspn and 1 μg/ml of ultra-LEAF™ CD44 function blocking antibody (Clone IM7; Biolegend) or equal amount of isotype control IgG2b kappa (Clone RTK4533 ...
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bioRxiv - Immunology 2020Quote: ... washed with PBS and stained for ASC using a primary antibody against the CARD domain (Biolegend; 1:1000 dilution) followed by an Alexa Fluor conjugated secondary antibody (Life technologies ...
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bioRxiv - Neuroscience 2021Quote: ... Primary antibodies included in this study were: mouse IgG2a-anti-β-III-tubulin (Tuj1, 1:400, catalog #801202, Biolegend), chicken-anti-NF200 (1:1000 ...
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bioRxiv - Neuroscience 2021Quote: ... the membranes were incubated overnight at 4°C with anti-Aβ primary antibody (6E10 Biolegend, SIG-39320, 1:1000) following incubation with goat anti-mouse secondary antibody (Thermo Scientific ...
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bioRxiv - Genomics 2019Quote: ... The resuspended cells were incubated for 20 min at 4 °C with the fluorophore-labeled antibodies CD45-PerCPCy5.5 (1:50) (Biolegend), Ly51-PE (1:800 ...
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bioRxiv - Immunology 2021Quote: ... and stained with 1 ug/test cell-hashing antibody (TotalSeq C0251, C0254, C0256, C0260, clones LNH-95, 2M2, BioLegend) for 30 minutes at 4C ...
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bioRxiv - Immunology 2021Quote: ... about 4 × 106 cells were stained with 1 μl mouse Fc-block (anti-CD16/32; BioLegend, San Diego, CA) in 50 μl FACS buffer (PBS containing 2% FBS ...
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bioRxiv - Systems Biology 2019Quote: ... and with biotinylated antibodies against a panel of bone marrow lineage markers (CD19, CD11b, CD11c, NK.1.1, Ter119, CD3ε, Gr-1 and B220 (BioLegend)) ...
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bioRxiv - Bioengineering 2019Quote: ... the cell morphological change was examined under bright field microscope and macrophage markers CD36 (2.5 µg per 1×106 cells in 100 µL; Cat. no. 108418, BioLegend) and CD71 (2.5 µg per 1×106 cells in 100 µL ...
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bioRxiv - Neuroscience 2019Quote: ... Cells were incubated overnight at 4°C with mouse anti-HA primary antibody (1:1000, Anti-HA.11, Biolegend), washed 3× in PBS and incubated with Alexa-conjugated anti-mouse secondary antibody (1:500 ...
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bioRxiv - Systems Biology 2019Quote: ... and with biotinylated antibodies against a panel of lineage markers (CD19, CD11b, CD11c, NK.1.1, Ter119, CD3ε, Gr-1 and B220 (BioLegend)) ...
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bioRxiv - Microbiology 2019Quote: ... membranes were reprobed after stripping of previously used antibodies using a pH 2.2 glycine-HCl/SDS buffer with anti-E.coli RNA Polymerase α (1:2,000; BioLegend 663102).
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bioRxiv - Immunology 2019Quote: ... in the presence of 1 μl BV421 anti-CD107a mAb for 2 hr at 37°C (H4A3, Biolegend 328626). Streptavidin M280 Dynabeads were conjugated to biotinylated HLA-C*05:01 refolded with P2-AV or P2-AW as described below.
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bioRxiv - Immunology 2021Quote: ... The cells were washed with PBS/BSA/EDTA and stained either with anti-IgM PE (dilution 1:100, Biolegend) and anti-IgG Alexa FluorTM 647 (dilution 1:600 ...
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bioRxiv - Systems Biology 2021Quote: ... After spinning them down the cells were incubated overnight at 4C with PerCP/Cy5.5 anti-BrdU (1:30 dilution) (BioLegend) in a buffer containing 0.5% Tween 20 / 1% BSA/PBS and RNase (0.8 mg/ml) ...
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bioRxiv - Immunology 2021Quote: ... the cells were fixed for 1 h at RT using the True-Nuclear transcription Factor Buffer Set (BioLegend, 424401). Following the fixation ...
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bioRxiv - Neuroscience 2021Quote: ... for 15 min and cells were stained with fluorophore-conjugated antibodies: CD11b BV605 (1:200, clone M1/70, Biolegend), CD45 PerCP-Cy5.5 (1:200 ...
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bioRxiv - Cancer Biology 2020Quote: Single cell suspension obtained by trypsin treatment was washed and incubated with an anti-mouse CD326 (EpCAM) AlexaFluor 647 antibody (1:2000, clone G8.8, Biolegend). DAPI (10 μg/mL ...
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bioRxiv - Cell Biology 2019Quote: ... Cell surface integrins were labelled with fluorochrome-conjugated antibodies [Alexa Fluor 488 anti-integrin β1 (Clone: HMβ1-1, Biolegend), APC anti-integrin α1 (Clone ...
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bioRxiv - Biochemistry 2021Quote: The following antibodies were used in this study: Anti-ZBP1 clone RonoB19 (WB 1:500, ref. 651602, Rat, BioLegend), anti-TIAR (C-18 ...
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bioRxiv - Biochemistry 2020Quote: ... Tetramers were prepared by mixing 1 or 5 pmol of biotinylated protein monomer with 0.25 to 1.25 pmol of streptavidin-PE (Biolegend #405245) respectively and incubating for 2 hours at room temperature ...
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bioRxiv - Cell Biology 2021Quote: Whole blood or washed platelets were labelled with anti-human BV421 CD62p (P-selectin; 1/100, AK4, BioLegend, 304926), BV421 CD42b(1/300 ...
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bioRxiv - Cancer Biology 2020Quote: ... The membrane was incubated in 5% dried non-fat milk powder dissolved in TBS-Tween 0,1% (TBST) for 1 h at room temperature (RT) and subsequently incubated with primary anti-HA antibody (Biolegend) followed by incubation by secondary goat HRP-conjugated anti-mouse IgG (Promega) ...
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bioRxiv - Cell Biology 2021Quote: ... were incubated with Calbryte 630TM (2μM; Stratech) for 30 minutes at 37°C followed by staining with CD42b-BV421 (1:100; Biolegend) for 15 minutes at room temperature and supplementation with 2mM CaCl2 ...
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bioRxiv - Microbiology 2021Quote: ... cells were incubated with 100 μl/well of the fixable dead cell viability dye Zombie Aqua (1:800, Biolegend) diluted in PBS for 30 min on ice ...
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bioRxiv - Cell Biology 2021Quote: 1×106 (for immunoblot) or 1.8×107 (for immunoprecipitation) Jurkat Cells were incubated with anti-CD3 (1 μg/ml, OKT3, 317325, BioLegend) and anti-CD28 (1 μg/ml ...
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bioRxiv - Genomics 2020Quote: ... Cells were then stained with a panel of 9 further DNA-oligonucleotide conjugated antibodies (0.33 μg/ml anti-CD71: BioLegend 113802 conjugated with ADT9 [CGAAGAAGGAGT], 1 μg/ml anti-CD41: BioLegend 133919 conjugated with ADT3 [TGTCCGGCAATA] ...
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bioRxiv - Immunology 2022Quote: ... neutrophils were incubated with autologous activated platelets (1:10 ratio) (65) for 2.5h and human recombinant CCL28 (50 nM) (BioLegend), the CCR3 antagonist SB328437 (10 mM ...
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bioRxiv - Immunology 2022Quote: ... SARS-CoV-1 biotinylated RBD protein (His & AVI Tag) (SinoBiological, 40634-V27H-B) conjugated with PE-streptavidin (BioLegend, 405204), SARS-CoV-2 biotinylated RBD protein (His & AVI Tag ...
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bioRxiv - Animal Behavior and Cognition 2022Quote: ... primary antibody 4G8 was incubated overnight at a 1:1000 dilution at room temperature (RT) (Biolegend, San Diego, CA). HRP-linked secondary goat anti-mouse antibody at a 1:1000 dilution (Invitrogen ...
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bioRxiv - Cell Biology 2022Quote: ... The cell suspension was stained using allophycocyanin (APC)/Cy7-conjugated rat anti-mouse CD45 antibody (1:200, BioLegend, #101319), PE-conjugated rat anti-mouse EpCam antibody (1:500 ...
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bioRxiv - Immunology 2022Quote: ... and the cells were resuspended in 200 μL of PBS supplemented with 1% FBS and containing 2.5 μL of Alexa Fluor 647-conjugated anti-human CD127antibody (BioLegend) or Alexa Fluor 647-conjugated mouse IgG1 k isotype control (BioLegend) ...
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bioRxiv - Neuroscience 2022Quote: ... and incubated at 4°C overnight with the following primary antibodies: mouse anti-C3 (1:200 dilution, 846302, Biolegend), rabbit anti-CD31 (1:200 dilution ...
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bioRxiv - Microbiology 2022Quote: The HA epitope was detected with mouse monoclonal antibody (mAb) HA.11 (diluted 1:1000) (BioLegend, item no. 901515) or rabbit polyclonal antibody (pAb ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were stained 20 min with APC-conjugated anti-CD24 antibody (1:100 dilution, 138506, Biolegend, San Diego, CA) and FITC-conjugated anti-Epcam antibody (1:500 dilution ...