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Citations for BioLegend :
6851 - 6900 of 7947 citations for Rat Caprin 1 CAPRIN1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: ... and myeloid cells were stained with Brilliant Violet 421-conjugated anti-CD11b antibody (1:100; Biolegend) for 1 hour in the dark with gentle agitation ...
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bioRxiv - Immunology 2024Quote: ... Supplementary Table 1) and 1ul/staining volume of hashtag TotalSeqC antibody mastermix (Biolegend, Supplementary Table 2) was added to each sample and cells were incubated for 30 minutes at 4°C ...
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bioRxiv - Cell Biology 2024Quote: ... live CD45+ cells were stained with the anti-mouse lineage negative cocktail (1:200; 133303 Biolegend), CD25 (1:250 ...
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bioRxiv - Neuroscience 2024Quote: ... anti-hyperphosphorylated neurofilament heavy chain (p-NF, mouse monoclonal, clone SMI 31P, from Biolegend, 1:100), Purified anti-Neurofilament H (NF-H) ...
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bioRxiv - Cancer Biology 2024Quote: ... incubated in FC-blocking mix (PBS containing human anti-CD16/CD32/CD64, 1:100 dilution, BioLegend) for 10 min at 4 °C and subsequently stained for surface markers in antibody mix in PBS for 30 min at 4 °C in the dark ...
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bioRxiv - Immunology 2024Quote: ... The following antibodies were used for staining: PerCP anti-mouse CD45 (Biolegend, 30-F11, 1:100) or BUV496 anti-mouse CD45 (BD ...
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bioRxiv - Bioengineering 2024Quote: Hydrogel full antibody stain included anti-I-A/I-E (1:800 dilution; FITC; BioLegend, 107605), anti-CD45 (1:800 ...
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bioRxiv - Immunology 2024Quote: ... for 30 mins and permeabilized with 1 × Intracellular Staining Permeabilization Wash Buffer (Cat No: 421002 Biolegend). The optimized concentrations of directly conjugated primary antibodies for intracellular antigen detection were added to samples and incubated for 30 minutes at 4 °C in the dark ...
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bioRxiv - Immunology 2024Quote: ... each knock-out was stained with a unique TotalSeqTM-A hashtag oligo (HTO) 1-10 (BioLegend) combination ...
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bioRxiv - Immunology 2024Quote: ... or FITC-labeled mouse anti human CD14 fluorescent antibody (cat. no. 301804; 1:1,000; BioLegend, Inc.) was added into each tube ...
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bioRxiv - Neuroscience 2024Quote: ... and then incubated in a primary antibody cocktail containing anti-SMI312 (Biolegend, 837904, 1:500 dilution), anti-MAP2 (Cell Signaling Technology ...
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bioRxiv - Immunology 2024Quote: ... Single-cell suspensions were then incubated with anti-mouse CD16/32 (1:100; clone 93; BioLegend) diluted in 2 mM EDTA/PBS to block Fc receptors at 4°C for 10 minutes ...
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bioRxiv - Immunology 2024Quote: ... 1 mM EDTA and FC block (Purified anti-mouse CD16/32, clone 93, BioLegend, Cat Nr101302) at 1:200 dilution at room temperature ...
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bioRxiv - Immunology 2021Quote: ... were determined using bead based immunoassays (CBA kit, Cat. 560484, BD PharMingen, San Diego, CA, USA; and LEGENDplex, Cat. 740003, BioLegend, San Diego, CA, USA). Log-transformed data were used to construct standard curves fitted to 10 discrete points using a 4-parameter logistic model ...
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bioRxiv - Cancer Biology 2021Quote: ... Further preparation for FACS measurement was following the protocol of the BioLegend APC Annexin V Apoptosis Detection Kit and DNA-staining with DAPI Reagent (25 μg/mL) (Biolegend, San Diego, CA, USA). 20 000 cells were assayed using a flow cytometer FACSCantoII (Becton Dickinson ...
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bioRxiv - Immunology 2021Quote: ... Naïve CD8+ T cells were then negatively-selected (CD8+CD44- purity > 90%) from the resulting single-cell splenocyte suspension using the MojoSort™ Mouse CD8 Naïve T Cell Isolation Kit (BioLegend, San Diego, CA, USA). Two modifications were introduced relative to the manufacturer’s guidelines to increase purity ...
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bioRxiv - Immunology 2024Quote: ... Single cell suspensions were labelled with antibodies against cell surface antigens as described above and then prepared using a Cyto-Fast Fix/Perm kit (BioLegend, San Diego, CA, US), according to the manufacturer’s instructions ...
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The loss-of-function of SOCS2 increases the inflammatory response to Staphylococcus aureus infectionbioRxiv - Immunology 2023Quote: ... Individual cytokine detection kits were also used to quantify mouse IL-6 and TNF-α (Bio-techne, France) and GM-CSF (Biolegend, San Diego, USA).
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bioRxiv - Bioengineering 2022Quote: ... cells were washed with DPBS and harvested with trypsin-EDTA and the viability analyzed by flow cytometry using the Apoptosis Detection Kit with propidium iodide (PI, BioLegend, San Diego, CA, USA) in a Cytoflex flow cytometer (Beckman Coulter ...
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bioRxiv - Cancer Biology 2023Quote: Flow cytometry viability staining was conducted by suspending murine spleen and tumor single cell suspensions in Zombie Violet fixable viability kit (Cat # 423114, BioLegend, San Diego, CA, USA) according to manufacturer instructions for 30 minutes at room temperature ...
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bioRxiv - Biophysics 2023Quote: ... Transduced T cells were positively selected with a PE selection kit (EasySep, ref: 17684) and an anti-HA PE-conjugated antibody (Biolegend, ref: 901518, clone: 16B12) at a 1:50 dilution to enrich transduced T cells ...
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bioRxiv - Immunology 2021Quote: ... 1-5 * 106 cells were stained with antibodies specific for surface markers Thy1.2 (clone 30-H12, BioLegend), Thy1.1 (clone OX-7 ...
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bioRxiv - Developmental Biology 2020Quote: ... Juvenile islet cells from SIX3-Flag pseudoislets were stained with anti-FLAG antibody-555 (1:100; Biolegend). Labeled cells were sorted on a special order 5-laser FACS Aria II (BD Biosciences ...
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bioRxiv - Neuroscience 2021Quote: ... mouse monoclonal anti-nonphosphorylated neurofilament H (clone SMI 32, Cat. # 801701, 1:1,000, BioLegend, San Diego, CA), and goat anti-choline acetyltransferase antibody (Cat ...
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bioRxiv - Molecular Biology 2020Quote: ... The specific antibodies used were anti-CD63 conjugated with FITC (CD63-FITC; 1:150, Cat. #: 353006, Biolegend); CD9-PE (1:1500 ...
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bioRxiv - Immunology 2022Quote: ... media was supplemented with 2 μg/ml of mouse IgG1 K anti-human PD-1 antibody (Biolegend) either alone or in combination with 1 μg/ml mouse IgG2 B anti-human TIGIT (R&D systems ...
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bioRxiv - Immunology 2021Quote: ... Alexa Fluor® 700 anti-human CD4 (clone RPA-T4; dilution 1:200; Biolegend; Cat no 344808), FITC anti-human CD8a (clone RPA-T8 ...
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bioRxiv - Cancer Biology 2019Quote: ... The cells were stained labeled with CD45 PE (Leukocytes) to minimize immune contamination (Biolegend #BL304008, 1:200), EPCAM/CD326 APC (Biolegend #324208 ...
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bioRxiv - Microbiology 2019Quote: ... Sputum samples were also stained with 1 µg/ml of PE anti-human CD45RO in PBS (BioLegend) for 30 min to stain activated and memory T cells ...
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bioRxiv - Physiology 2019Quote: ... and stained with biotinylated antibodies against CD11b to identify myeloid cells (1:1000, catalog number 101204, BioLegend), CD3 to identify T cells (1:250 ...
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bioRxiv - Immunology 2019Quote: ... and the following day were restimulated with either 1 μg/ml each of anti-CD3 (HIT3α, Biolegend) or PMA and ionomycin (Biolegend ...
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bioRxiv - Immunology 2021Quote: ... IFN-β and IFN-γ were determined using the LEGENDplex mouse type 1/2 interferon panel (BioLegend) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: ... resuspended them in staining solution containing 1:200 PE goat anti-mouse IgG secondary antibody (#405307, BioLegend) and 1:1000 Hoechst 33342 (Thermo Fisher ...
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bioRxiv - Bioengineering 2021Quote: ... which were incubated overnight at 4 °C with primary antibodies diluted 1:5,000 in blocking buffer (BioLegend Way ...
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bioRxiv - Microbiology 2020Quote: ... Immunostaining was performed with primary antibodies for one hour at room temperature for HA (1:1000, BioLegend) or rabbit anti-IFITM3 (Cat ...
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bioRxiv - Genomics 2021Quote: ... Cells were stained on ice with anti-murine-CD45-PE (mCD45)(clone 30-F11; BioLegend; 1:20) in the dark for 30 mins ...
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bioRxiv - Microbiology 2021Quote: ... and stained intracellularly in 100 μl/well of anti-mouse IFN-γ (clone XMG1.2, 1:200, Biolegend) plus anti-mouse TNF-α (clone MP6-XT22 ...
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bioRxiv - Cell Biology 2020Quote: Erythroid progression was followed by immunostaining with phycoerythrin (PE)–conjugated anti-human CD235a (1:100) (116207, BioLegend) and Allophycocyanin (APC)–conjugated anti-CD71 (1:100 ...
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bioRxiv - Cancer Biology 2021Quote: ... before proceeding with intra-cellular cytokine staining using antibodies specific for INF-γ (4S.B3, 1:40) (Biolegend) IL-2 (MQ1-17H12 ...
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bioRxiv - Neuroscience 2022Quote: ... RGCs were fixed and immunostained with neuronal marker βIII-Tubulin (1:500; mouse IgG2a, MMS-435P, BioLegend) and DAPI (1:5000 ...
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bioRxiv - Immunology 2022Quote: Cells were stained with Annexin V-Alexa 647 or Annexin V-Pacific Blue (both 1:30, BioLegend) in Annexin binding buffer (10 mM HEPES ...
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bioRxiv - Neuroscience 2022Quote: ... cell suspensions were incubated with the following antibody cocktail: CD45-BV650 (BioLegend, San Diego, CA; 1:200), Ly6G-PECY7 (BioLegend ...
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bioRxiv - Molecular Biology 2022Quote: ... The following conjugated antibodies were used: FITC-conjugated anti- mouse Ly6d (1:100, BioLegend, clone 49-H4). All protocols and primary ...
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bioRxiv - Cancer Biology 2022Quote: ... Biotin-conjugated antibodies were visualized by secondary staining with streptavidin PE/Cyanine5 (BioLegend 405205, dilution 1:200). Antibodies used for staining of human tumor cells included ...
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bioRxiv - Cancer Biology 2022Quote: ... Approximately 1×106 cells were aliquoted in 5 ml tubes and incubated with Fc Blocking antibody (Biolegend). Antibodies used for immune profiling are detailed in Supplementary Table 1 ...
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bioRxiv - Developmental Biology 2022Quote: ... followed by the addition of antibodies for 30-minutes on ice: VEGFR2-PECy7 (Biolegend, #136414, 1:100), CD45-APC (Biolegend ...
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bioRxiv - Cancer Biology 2022Quote: ... and the blood was further diluted at 1:100 ratio in the cell staining buffer (Biolegend, 420201). The cells were stained with CD45 flow antibody and proceeded for flow cytometry to analyze the frequency of CD45+ immune cells.
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bioRxiv - Developmental Biology 2021Quote: ... the cells were incubated with APC-conjugated anti-SUSD2 antibody (1:20, clone W5C5, Cat. 327401, BioLegend) for 30 min in culture medium ...
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bioRxiv - Biochemistry 2021Quote: ... Cells were resuspended in 25 μL block solution (wash plus 1 in 40 Human TruStain FcX – Biolegend) and incubated for 5 min ...
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bioRxiv - Microbiology 2020Quote: ... the cells were stained for 1 h with 0.01 mg/ml goat anti-mouse Dylight 488 (Biolegend), 0.01 mg/ml donkey anti-rabbit AF555 (Biolegend) ...