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Citations for BioLegend :
6651 - 6700 of 6837 citations for TGFB3 Human Mouse Rat HEK293 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: Fc block was performed using TruStain FcX™ CD16/32 antibody (anti-mouse) to avoid non-specific binding (101320 Biolegend, UK) according to the supplier protocols The fluorochrome-conjugated antibodies used in the panel were obtained from Biolegend ...
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bioRxiv - Immunology 2020Quote: ... was incubated with 1-2 spike+ cells for 1 hour at 4°C before adding of 0.02 ug/ml ACE2-mFc followed by FITC conjugated anti-mouse IgG (Biolegend, cat#405305) and the MFI of FITC and positive percentage were collected.
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bioRxiv - Immunology 2020Quote: ... Plates were washed three times with PBS-T (PBS with 0.1% Tween-20) and 50μl of mouse IgG-specific secondary antibody (BioLegend #405306, 1:10,000) diluted in dilution solution added to each well ...
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bioRxiv - Immunology 2021Quote: ... and surface TCR expression was confirmed via flow cytometry on BD Fortessa using FACSDiva software using antibodies against mouse TCRβ constant region (APC-Fire750-conjugated, Biolegend 109246), clone H57-597 ...
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bioRxiv - Immunology 2022Quote: ... FcBlock was added for 10 min prior to fluorescent antibody staining for surface proteins for 30 min at 4°C using the following antibodies: BV510 anti-mouse CD45 (Biolegend 103137), FITC anti-mouse CD11b (eBioscience 11-0112-86) ...
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bioRxiv - Cell Biology 2022Quote: ... The remaining cells were incubated with Biotin anti-mouse Ly-6A/E (Sca-1) antibody (Biolegend; Cat. No. 122504; 1:75) at 4°C for 20 minutes then 10 µl Streptavidin beads (1:30 ...
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bioRxiv - Cancer Biology 2022Quote: ... The washed samples were stained for 20 min on ice with Brilliant Violet 510™ anti-mouse CD45 Antibody (1:200, Clone: 30-F11, BioLegend), PE/Cyanine7 anti-mouse/human CD11b Antibody (1:200 ...
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bioRxiv - Bioengineering 2022Quote: ... fluorescence minus one (FMO) + isotype control antibody staining was performed using the following isotypes: PE/Cy7 Mouse IgG2a κ (cat # 400232, BioLegend), BV-421 Mouse IgG1 κ (cat # 400158 ...
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bioRxiv - Immunology 2022Quote: ... for 15 minutes and subsequently stained with a combination of fluorophore-conjugated primary antibodies against mouse CD45 (BioLegend clone 30-F11), CD11b (BioLegend clone M1/70) ...
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bioRxiv - Immunology 2022Quote: ... T cells at 1×106/ml were kept in culture withdraw of cytokines for 8 hours before stimulated with 1µg/ml plate-coated anti-mouse CD3e (Biolegend, Cat.100340) and 1µg/ml soluble anti-mouse CD28 (Biolegend ...
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bioRxiv - Immunology 2022Quote: The following protocol was used for all tumor experiments: after the Fc receptor of the cells was blocked with anti-mouse CD16/32 (Biolegend, 101320), extracellular staining was performed in FACS buffer for 30 minutes ...
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bioRxiv - Microbiology 2022Quote: The amount of TNFα secreted in the supernatants from cell culture and cell lysate was determined by ELISA MAX™ Deluxe Set Mouse TNFα kit (BioLegend) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... Cells were then stained with fluorescent antibodies for intracellular proteins for 30 min at 4°C using the following antibodies: PE anti-mouse TNF (Biolegend 506306) and APC anti-mouse IL-6 (Biolegend 503810) ...
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bioRxiv - Neuroscience 2023Quote: ... Membranes were incubated in primary antibodies diluted in 5% skim milk in TBST: mouse anti-brevican (1:1000, BioLegend, cat# 820101), sheep anti-neurocan (1:1000 ...
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bioRxiv - Immunology 2022Quote: ... samples were pre-incubated for 5 minutes at 4°C with TruStain FcX™ (anti-mouse CD16/32) Fc block (clone 93; BioLegend). Samples were then stained for extracellular markers in the presence of Fc block for 30 minutes at 4°C protected from light using the following antibodies ...
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bioRxiv - Neuroscience 2022Quote: ... Then the live microglial cells were incubated with PBS supplemented with 5% BSA + HLA-DR mouse monoclonal antibodies pre-conjugated with FITC (1:100, BioLegend, Inc.) + Hoechst 33342 (1:5000 ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: OVA-specific IgE levels were measured using a LEGEND MAX™ Mouse OVA Specific IgE ELISA Kit (BioLegend, San Diego, CA). The plate was washed 4 times with wash buffer followed by addition of Matrix A to standard wells and Assay Buffer to sample wells ...
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bioRxiv - Cancer Biology 2022Quote: ... Tissue sections were stained with monoclonal mouse anti-βIII-tubulin primary antibody (Biolegend, Cat. 801202; 1:100 overnight at 4 °C) and goat anti-mouse AF647 (abcam ...
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bioRxiv - Cancer Biology 2022Quote: ... then incubated with their respective primary antibodies diluted in 5% skim milk in PBST at 4 °C overnight: purified mouse anti-βIII-tubulin (1:1,000) (BioLegend, cat. 801202), monoclonal mouse anti-GAPDH (1:50,000 ...
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bioRxiv - Cell Biology 2024Quote: ... The cytokine capture bead for the infectious disease workflow was generated by conjugating an anti-mouse TNFα antibody (#6B8, BioLegend, UK) to 6.7 μm carboxyl polystyrene beads according to Spherotech guidelines.
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bioRxiv - Immunology 2023Quote: ... CD4 T cells were isolated from the spleen and LNs of indicated naïve OT-II strains with Mouse CD4 T Cell Isolation Kit (STEMCELL Technologies 19852 or BioLegend 480033) and labeled with 1.0 µM CFSE (Thermo Fisher ...
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bioRxiv - Molecular Biology 2024Quote: ... Western blotting was performed using an anti-HA mouse monoclonal antibody (Alexa Fluor® 488 anti-HA.11 Epitope Tag Antibody, BioLegend) as primary antibody diluted 1:1000 in TBS-T (20 mM Tris ...
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bioRxiv - Genetics 2024Quote: ... was collected in EDTA-coated capillary and was stained for 15 min in the dark at room temperature with APC labelled anti-mouse CD45.1 antibody (Biolegend, 1:20) and PE labelled anti-mouse CD45.2 antibody (Biolegend ...
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bioRxiv - Immunology 2024Quote: ... Cells were next stained for 30 mins on ice with a 1:300 dilution of antibodies raised against mouse CD206 (Biolegend,141710), CD301(Biorad ...
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bioRxiv - Immunology 2024Quote: ... CORSET8 T cells were purified from the TCR Tg donor mice with the MojoSort® Mouse CD8 T Cell Isolation Kit (Biolegend) and labelled with Cell Proliferation Dye eFluor 450 (0.01 mM ...
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bioRxiv - Immunology 2024Quote: ... The LEGENDplex™ Mouse Immunoglobulin Isotyping Panel catalog #740493 with the V-bottom plate was used according to manufacturer’s instructions (BioLegend, CA). Briefly ...
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bioRxiv - Immunology 2024Quote: ... Lymph node cells were harvested from TCR Tg donor mice and CD8+ T cells were purified with MojoSort® Mouse CD8 T Cell Isolation Kit (Biolegend) and labelled with Cell Proliferation Dye eFluor 450 (0.01 mM ...
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bioRxiv - Neuroscience 2023Quote: ... brains were incubated with 1:1000 chicken anti-GFP antibody (Aves GFP-1020) and 1:1000 mouse anti-tyrosine hydroxylase antibody (BioLegend 818001), with boosters over 5 days for a final concentration of 1:200 ...
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bioRxiv - Bioengineering 2024Quote: ... for 10 min at room temperature and then stained for 30 min at 4°C with the following antibody cocktail: anti-mouse CD45.1 (Biolegend, cat.: 110735), anti-mouse TER-119 (eBiosciences ...
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bioRxiv - Microbiology 2023Quote: ... The membrane was incubated in 1% milk-PBS-T containing rabbit anti-LpxC antibody (a generous gift from the Doerrler lab) or mouse anti-RpoA (anti-E.coli RNA polymerase alpha from Biolegend, cat# 663104) diluted 1:10,000 ...
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bioRxiv - Immunology 2023Quote: ... The samples were washed with Cell Staining Buffer and stained with anti-mouse TCR β chain-Alexa Fluor® 647 (Biolegend) at a concentration of 0.02μg/μl ...
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bioRxiv - Microbiology 2023Quote: Lung single-cell suspensions were incubated with TruStain FcX™ (anti-mouse CD16/32) antibody (Cat: 101320; BioLegend, San Diego, CA) in eBioscience™ Flow Cytometry Staining Buffer (Cat ...
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bioRxiv - Cancer Biology 2023Quote: Plasma Ig were analysed using the LEGENDplex Mouse Immunoglobulin Isotyping panel (6-plex) by flow cytometry (Cat #740493, BioLegend, CA, USA). Plasma sample and standards was prepared using assay buffer as per the manufacturer’s instruction ...
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bioRxiv - Cancer Biology 2023Quote: ... centrifuged and 500,000 cells were stained in 200 µl 1x PBS plus 0.5 µg of indicated antibody for 30 min in the dark: APC anti-mouse CD274 (B7-H1, PD-L1, Biolegend #124311) or appropriate IgG isotype ...
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bioRxiv - Physiology 2023Quote: The levels of TNF-α and IL-6 in the serum and the supernatant of the crushed muscle tissues were measured using a commercial kit (mouse TNF-α and IL-6 ELISA max standard set, BioLegend). The values of TA and GA muscles were revised to the protein concentration measured by BCA kit and expressed as pg/mg.
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bioRxiv - Neuroscience 2023Quote: ... Primary antibodies were using 1:500 rabbit anti Abeta 1:500 (IBL, CAT # 18584) and 1:500 mouse anti SMI312 (Biolegend 837904).
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bioRxiv - Neuroscience 2023Quote: ... Membranes were incubated with primary anti-HA antibodies diluted in PBS-tween 0.2% blocking medium (mouse anti-HA, 1:5000, Biolegend cat. # 901513) over night at 4°C under agitation ...
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bioRxiv - Immunology 2023Quote: Levels of CXCL9 and CXCL10 were measured in plasma (diluted 2-fold) using the mouse cytokine release syndrome panel LEGENDplex system (BioLegend, UK), according to manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... Single-cell suspensions were generated by passing through 70 μm filters and sequentially stained with APC anti-mouse CD45 (Biolegend #103111) and propidium iodide (Sigma ...
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Mice generated with induced pluripotent stem cells derived from mucosal-associated invariant T cellsbioRxiv - Immunology 2023Quote: The quantification of cytokines was performed with the LegendPlex mouse Th cytokine panel according to the protocol provided by the manufacturer (Biolegend, USA). The amounts of cytokine per cell were calculated as follows ...
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bioRxiv - Immunology 2023Quote: ... we incubated sequential de-paraffinized sections with anti-2’,3’-cyclic-nucleotide 3’-phosphodiesterase (CNPase) (mouse CNPase: 1:200 dilution; Biolegend, Inc.), anti-CD3 (rabbit ...
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bioRxiv - Microbiology 2023Quote: ... Supernatants were at 8 h post-infection and used fresh or stored at -20°C for up to 1 week and TNF was quantified using mouse TNF-α ELISA MAX kit (BioLegend) following manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... Tyrosine hydroxylase was visualized using an Alexa Fluor® 594 conjugated mouse monoclonal anti-TH antibody at a 1:500 concentration (818004; Biolegend). Glutamine synthetase was visualized using a mouse monoclonal anti-GS antibody at a 1:100 concentration (MA5-27750 ...
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bioRxiv - Developmental Biology 2023Quote: ... anti-Neurod1 (1/500, mouse monoclonal, hybridoma) (Kani et al., 2010), anti-paired box 2 [Pax2] (1/700, rabbit polyclonal) (BioLegend, 901001), anti-Foxp1b ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... was used to mark single cells of DMSO treated mice and TotalSeq™-B0304 anti-mouse Hashtag 4 Antibody (Clone M1/42; 30-F11; 155837; BioLegend) was used for aortic cells of TRAF-STOP treated mice ...
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bioRxiv - Immunology 2023Quote: The following list of antibodies and their concentration against mouse proteins were employed: αCD45 (1:400; BioLegend Cat# 103147, RRID:AB 2564383), αCD3ε (1:300 ...
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bioRxiv - Developmental Biology 2023Quote: ... Then cells were again washed and followed by an incubation with anti-mouse IgG coupled to PE-Cy7 (Biolegend ref 406613) at a 1/200 dilution in BD wash buffer during 20 min at room temperature ...
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bioRxiv - Microbiology 2023Quote: ... The membranes were then incubated in 1×PBST with either rabbit anti-flagellin (kindly given by Dr Monica Rolando) or mouse anti-RNAPα (BioLegend #663104) diluted 1/5,000 for 1h at room temperature with agitation ...
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bioRxiv - Immunology 2023Quote: ... and cellular surface markers were stained with the following fluorophore-conjugated anti-mouse antibodies: Alexa Fluor 488 CD3 (BioLegend, Cat# 100210), Alexa Fluor 488 CD19 (BioLegend ...
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bioRxiv - Bioengineering 2023Quote: Secreted cytokine levels into the collected IKKβCA- and Control-CMs were measured using 9-Plex LEGENDPlex mouse inflammation panel (BioLegend, Cat #740446) according to manufacturer’s protocol ...