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Citations for BioLegend :
601 - 650 of 9381 citations for T Cell Surface Glycoprotein CD3 Gamma Chain CD3G Antibody Biotin since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... Primary antibodies used were as follows: purified Armenian Hamster anti-mouse T cell receptor γ/δ (BioLegend, 118101), purified rat anti-mouse MHC class II or MHC class II Alexa 488 (BioLegend ...
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bioRxiv - Bioengineering 2022Quote: ... CD8 T cells were >99% pure and assessed by flow cytometry staining with CD8-Pacific Blue antibody (Biolegend). Total CD8 T cells were activated using anti-CD3/CD28 coated beads (Invitrogen ...
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bioRxiv - Microbiology 2023Quote: ... An early activation marker of T cells was also monitored using PE-Conjugated CD-69 antibody (Biolegend, 310906). Analysis was performed on FlowJo software ...
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bioRxiv - Cell Biology 2024Quote: ... or rabbit polyclonal antibody against non-muscle myosin II heavy chain A (BioLegend; catalog #PRB-440P)) diluted in 5% bovine serum albumin (BSA ...
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bioRxiv - Immunology 2023Quote: Activation consisted of 10μg/mL plate-bound anti-human CD3 antibody (300437) and 1μg/mL soluble anti-human CD28 antibody (302902) purchased from BioLegend, USA ...
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bioRxiv - Microbiology 2019Quote: ... as assessed by measuring the percentage of CD3+/CD4+ cells via flow cytometry with FITC-anti-CD3 (Biolegend, San Diego, CA, catalogue #317306) and APC-anti-CD4 (Biolegend ...
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bioRxiv - Neuroscience 2019Quote: ... Red blood cells were osmotically lysed and CD4+ T-cells were isolated using a MojoSort Mouse CD4+ T Cell Isolation Kit (Biolegend, San Diego, CA). Purified CD4s were labeled with Cell Proliferation Dye eFluor450 (ThermoFisher ...
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bioRxiv - Immunology 2021Quote: ... and donor-derived CD4 T cells were analyzed by flow cytometry following magnetic bead–enrichment with MojoSort Mouse CD4 T Cell Isolation Kit (BioLegend).
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bioRxiv - Immunology 2021Quote: ... CD4 T cells from the spleen and peripheral lymph nodes of C57BL/6 mice on 22-24 dpi with LCMV-c13 were harvested and enriched for CD4 T cells using a MojoSort Mouse CD4 T Cell Isolation Kit (BioLegend) prior to surface staining ...
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bioRxiv - Immunology 2022Quote: ... Naïve CD4+ T cells were isolated using the Mojosort Mouse CD4 Naïve T Cell Isolation Kit (Biolegend, San Diego, CA) using LS columns on a QuadroMACS magnet (Miltenyi Biotec ...
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bioRxiv - Microbiology 2021Quote: ... Resting CD4+ T cells were isolated from total PBMCs by negative selection using the MojoSort Human CD4+ T Cell Isolation kit (Biolegend) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... CD8+ T cells were then isolated from the spleens using the MojoSort™ Mouse CD8 T Cell Isolation Kit (BioLegend), following the manufacturer’s guidelines ...
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bioRxiv - Bioengineering 2023Quote: ... binding of T cell-secreted SiRPα monomer on T cell CD47 was assessed indirectly by staining with anti-human CD47 (CC2C6, Biolegend) at 4°C for 30min ...
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bioRxiv - Immunology 2023Quote: ... CD45.1+ GFP+ OT-II CD4+ T cells were purified from mouse spleens using magnetic beads (CD4 T cell MojoSort isolation kit, Biolegend) and activated with 10μg/ml recombinant mouse IL-2 IS (Miltenyi ...
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bioRxiv - Immunology 2020Quote: ... cells were stained with APC/Cyanine7 anti-human CD3 (Clone SK7, Biolegend), PE/Dazzle™ 594 anti-human CD4 (Clone RPA-T4 ...
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bioRxiv - Genetics 2020Quote: ... We stimulated Jurkat cells with 5 μg/ml anti-CD3 (Biolegend-317315) and 100 ng/ml phorbol 12-myristate 13-acetate (PMA ...
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bioRxiv - Immunology 2020Quote: ... Cells were activated using 1ug/ml anti-CD3 (Clone: 145-2C11, Biolegend) and anti-CD28 (Clone ...
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bioRxiv - Immunology 2021Quote: ... Cells were activated with 1 μg/mL anti-CD3 (clone HIT3α, BioLegend) and 1 μg/mL anti-CD28 (clone CD28.2 ...
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bioRxiv - Immunology 2023Quote: ... Cells were activated using 1ug/ml anti-CD3 (Clone: 145-2C11, Biolegend) and anti-CD28 (Clone ...
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bioRxiv - Immunology 2023Quote: ... cells were incubated with mouse anti-human CD3 (clone UCHT1, Biolegend #300402), anti-human CD4 (clone RPA-T4 ...
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bioRxiv - Immunology 2020Quote: ... Biotin-labeled antibodies were visualized with fluorochrome-conjugated streptavidin (Thermo Fisher, or BioLegend). LIVE/DEAD™ Fixable Aqua Dead Cell Stain Kit ...
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bioRxiv - Immunology 2021Quote: ... with addition of a biotin anti-mouse CD25 antibody (0.5 μg/mL; BioLegend). Magnetically-enriched CD4+ T cells were cultured in (DMEM ...
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bioRxiv - Immunology 2021Quote: ... hamster sera with biotin goat anti-hamster (Syrian) IgG antibody (BioLegend, Cat: 405601) followed by incubation with HRP-Streptavidin (BD ...
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bioRxiv - Cell Biology 2022Quote: ... Biotin-conjugated antibodies were then stained with Pacific blue-conjugated streptavidin (Biolegend 405225). DAPI (BD Pharmigen 564907 ...
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bioRxiv - Immunology 2021Quote: ... The cells were stimulated with 20μg/ml of a purified F(ab’)2 goat anti-mouse IgM (μ chain) antibody (BioLegend, Clone Poly21571, Cat# 157102) at 37°C for 10 seconds ...
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bioRxiv - Immunology 2020Quote: ... For investigating the formation of iBALT B cell and T cells were stained in lungs using primary antibody cocktail containing rat anti-mouse CD45R/B220 (BioLegend, Cat#103202) and Hamster anti mouse CD3 (BD ...
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bioRxiv - Immunology 2022Quote: ... and the MojoSort Human CD8 T cell (BioLegend) isolation kit ...
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bioRxiv - Immunology 2023Quote: ... or MojoSortTM CD4 T cell isolation kit (BioLegend), before being purified by FACS ...
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bioRxiv - Cancer Biology 2022Quote: HLA surface expression was assessed using pan-HLA-A/B/C antibody (BioLegend, W6/32), anti-HLA-A03 (ThermoFisher Scientific ...
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bioRxiv - Immunology 2020Quote: ... The surface staining antibody master-mix included: 1:25 of anti-CD4 BV711 (OKT4, BioLegend), 1:100 of anti-CD8 APC-H7 (SK1 ...
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bioRxiv - Immunology 2019Quote: ... and then stained with fluorescent-conjugated antibodies for surface markers: Alexa700 anti-mouse CD45 (BioLegend), APC-Cy7 anti-mouse CD11b (BioLegend ...
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bioRxiv - Neuroscience 2022Quote: ... The antibodies that are specific for surface markers included CD103 Brilliant Violet 421 (121422; BioLegend), NK1.1 Pac Blue (108722 ...
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bioRxiv - Immunology 2023Quote: ... Surface antibodies used in this study were: BUV661 anti-mouse CD45 (103147, BioLegend, 1:100); PeCy7 anti-mouse CD4 (100422 ...
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bioRxiv - Immunology 2024Quote: ... we used anti-human antibodies for surface-marker staining: anti-CD45 (BV785, clone HI30 – BioLegend), anti-CD3 (Alexa700 ...
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bioRxiv - Cancer Biology 2022Quote: ... Antibodies used for removal of mouse stromal cells included: anti-mouse H-2Kd-biotin (BioLegend, 116604, clone SF1.1, dilution 1:20) and anti-mouse Cd45-PE/Cyanine5 (BioLegend 103110 ...
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bioRxiv - Cell Biology 2022Quote: ... The remaining cells were incubated with Biotin anti-mouse Ly-6A/E (Sca-1) antibody (Biolegend; Cat. No. 122504; 1:75) at 4°C for 20 minutes then 10 µl Streptavidin beads (1:30 ...
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bioRxiv - Molecular Biology 2019Quote: ... CD25 surface expression were further confirmed by staining the transduced HEK293T cells with anti-CD25 Brilliant Violet 605 antibody (BioLegend, Clone BC96, #302632). Fluorescence was monitored by flow cytometry and was compared to non-transduced HEK293T cells (data not shown) ...
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bioRxiv - Immunology 2022Quote: ... Antibodies used for sorting were: anti human CD3-BV510 (Biolegend, San Diego, California, United States), CD4-FITC (eBioscience ...
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bioRxiv - Immunology 2019Quote: ... at RT in the dark using the following antibodies: anti-CD3–PerCP (clone OKT3; BioLegend), anti-CD19– Brilliant Violet 510 (clone HIB19 ...
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bioRxiv - Cancer Biology 2021Quote: ... They were then activated by incubating them with anti-human CD3 antibody (5μg/ml) (Biolegend) and anti-human CD28 antibody (5μg/ml ...
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bioRxiv - Cancer Biology 2022Quote: ... 96-well plates were coated in anti-CD3 antibody (1 μg/ml Biolegend, cat #100340), and splenocytes from CD-1 nude mice were added as antigen presenting cells (APCs) ...
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bioRxiv - Microbiology 2020Quote: ... for 30 min on ice with an APC-labelled antibody to CD3 (Biolegend; clone UCHT1), phycoerythrin (PE)-Cy7-labelled antibody to CD4 (Biolegend ...
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bioRxiv - Immunology 2020Quote: ... The second antibody master mix used included 1:50 of anti-CD3 BV650 (OKT3, BioLegend), 1:50 of anti-CD4 BV711 (OKT4 ...
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bioRxiv - Immunology 2020Quote: ... Read out of proliferation is performed with the following antibodies: CD3-PerCP/Cy5.5 (UCHT1; Biolegend), CD4-FITC (RPA-T4 ...
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bioRxiv - Cancer Biology 2022Quote: ... Alexa Fluor 647 anti-human CD3 Antibody (#300322, Mouse IgG2a κ, dilution 1:100, Biolegend); APC Rat IgG2a κ Isotype Ctrl (#400512 ...
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bioRxiv - Cell Biology 2023Quote: ... The mixture was then stained with Alexa Fluor 488 anti-human CD3 antibody (BioLegend, 300320), PE anti-human CD25 antibody (BioLegend ...
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bioRxiv - Immunology 2023Quote: Antibodies targeting the following molecules were used in this study: CD3 (clone:HIT3a; PerCP/Cy5.5 BioLegend, clone:UCHT1 ...
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bioRxiv - Immunology 2023Quote: ... and stained for 5 min at 4°C with biotinylated anti-CD3 antibody (17A2; BioLegend) to exclude CD3-positive T cells in the cell suspension using anti-biotin microbeads (Miltenyi Biotec ...
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bioRxiv - Systems Biology 2023Quote: ... Plates were coated with 3.33 μg/mL α-CD3 (HITa3) and α-CD28 antibodies (Biolegend), in 100 μL PBS at 4°C overnight ...
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bioRxiv - Microbiology 2023Quote: ... PBMC samples were stained by the following antibodies: CD3-Brilliant Violet 605 (clone OKT3, BioLegend), CD4-PerCP-Cy5.5 (clone OKT4 ...