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Citations for BioLegend :
601 - 650 of 5309 citations for Mouse VPS10 domain containing receptor SorCS1 SORCS1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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Cholesterol deprivation induces TGFβ signaling to promote basal differentiation in pancreatic cancerbioRxiv - Cancer Biology 2019Quote: ... To prevent antibodies from binding to Fc-receptors we treated the cell suspension with Fc-block (Biolegend #101301; 1:50) prior using other antibodies ...
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bioRxiv - Immunology 2020Quote: ... Cells were resuspended in their residual buffer before being incubated with anti-CD16/CD32 (‘Fc Block’) to reduce non-specific binding to Fc receptors (Biolegend). After 5 min ...
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bioRxiv - Immunology 2021Quote: ... Single cell suspensions were blocked with rat anti-mouse FcγIII/II receptor (CD16/CD32) blocking antibodies (“Fc-Block”) and stained with live/dead cell-exclusion dye (Zombie UV dye; Biolegend). The cells were then incubated with fluorophore-conjugated antibodies directed against cell surface antigens ...
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bioRxiv - Molecular Biology 2023Quote: ... 10 million cells were resuspended in 1 mL of FACS buffer and 50 μL of Human TruStain FcX Fc Receptor Blocking Solution (BioLegend) was added and left to bind the cells on ice for 15 minutes ...
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bioRxiv - Immunology 2023Quote: ... Cells were incubated with anti-CD16/CD32 (clone 93, eBioscience, 14-0161-86) for Fc receptor blockade and stained with Zombie Yellow (Biolegend) for 10 minutes at room temperature in the dark to distinguish live and dead cells ...
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bioRxiv - Genomics 2023Quote: ... Cells were pre-incubated with 5 µl of Human TruStain FcX™ (Fc Receptor Blocking Solution, BioLegend Cat. No. 422301) per 100µl of cell suspension for 5–10 minutes at room temperature ...
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bioRxiv - Immunology 2023Quote: ... single cell suspensions were incubated on ice for 10 minutes with Fc receptor-blocking anti-CD16/32 (BioLegend Cat# 101301). Cell suspensions were first stained for 5 minutes at room temperature with LIVE/DEAD® Fixable Red Dead Cell Stain Kit (ThermoFisher Cat# L23102) ...
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bioRxiv - Immunology 2024Quote: ... antibody or 100µL of PBS containing 0.2µg TruStain FcX™ and 0.04µL Zombie AquaTM Fixable Viability Kit (BioLegend cat#: 423102) and incubated at room temperature (RT ...
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bioRxiv - Cancer Biology 2020Quote: ... and plasma were assayed for CXCL5 using a human/mouse CXCL5 immunoassay kit (BioLegend, 440904).
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bioRxiv - Developmental Biology 2019Quote: ... placental single-cell suspensions were stained with APC-conjugated anti-mouse CD117 (c-kit) (BioLegend), PerCP/Cy5.5-conjugated anti-mouse CD34 (BioLegend) ...
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bioRxiv - Immunology 2020Quote: All MPC experiments in this protocol were performed with Biolegend LEGENDScreen Mouse PE Kit (Biolegend) and manufacturer recommendations for LEGENDScreen Plate preparation and staining were performed as indicated by the manufacturer ...
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bioRxiv - Molecular Biology 2020Quote: ... 13) were assayed using LEGEND plex Mouse Th1/Th2 panel kits (BioLegend, San Diego, USA) according to the manufacturer’s instructions.
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bioRxiv - Immunology 2023Quote: ... Neutrophils were isolated by negative selection using the Mojosort Mouse Neutrophil Isolation Kit (Biolegend 480058). Wells were pre-coated with 100 μL per well of capture anti-histone antibody contained in the Cell Death Detection ELISA kit (1:40 in 1x coating buffer ...
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bioRxiv - Immunology 2023Quote: ... Neutrophils were then sorted by negative selection using Mojosort Mouse Neutrophil Isolation Kit (Biolegend 480058) and stained with 100 nM dichlorofluorescein diacetate (DCFDA ...
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bioRxiv - Immunology 2023Quote: ... CD8+ T cells were isolated using MojoSort Mouse CD8 T Cell Isolation Kit (480035, BioLegend). CD8+ T cells were cultured and expanded in a similar fashion to γδ T cell populations ...
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bioRxiv - Immunology 2024Quote: ... The MojoSort Mouse Naïve CD4+ T cell Isolation Kit (#480040, BioLegend, San Diego, CA, USA) was used for the isolation of naïve CD4+ T cells as previously described (15) ...
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bioRxiv - Immunology 2024Quote: ... CD4+ cells were purified with the MojoSortTM mouse CD4 T cell isolation kit (Biolegend, 480006) according to manufacturer instructions ...
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bioRxiv - Cell Biology 2020Quote: ... for 10 minutes at 4°C followed by staining with antibody cocktail containing Pacific-Blue-conjugated anti-mouse CD45 (clone 30-F11; Biolegend, San Diego, CA, USA), PE-conjugated anti-mouse Ly-6G (Gr-1 ...
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bioRxiv - Neuroscience 2024Quote: ... flow cytometry staining master mix was prepared containing 1:200 dilutions of PE anti-mouse/human CD11b Antibody (BioLegend, Cat#:101208, clone M1/70), APC anti-mouse CX3CR1 (Antibody BioLegend ...
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bioRxiv - Immunology 2022Quote: ... Tissue and serum samples were loaded directly onto ELISA plates according to protocol (Biolegend 439407 Legend Max Mouse IFN-B ELISA kit) ...
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bioRxiv - Immunology 2021Quote: ... The levels of IL-17 were measured by using ELISA MAXTM Deluxe Sets (BioLegend).
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bioRxiv - Immunology 2019Quote: ... Culture supernatants were collected and analyzed for IFNg and IL-2 by ELISA (BioLegend).
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bioRxiv - Bioengineering 2021Quote: ... IFNγ concentrations in cell culture supernatants were determined after 48h by ELISA (Biolegend # 430104) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... The supernatants were collected and analyzed by murine IFNg and IL-17A ELISA (BioLegend). Spleens were processed similar to the lungs without the need of enzymatic digestion.
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bioRxiv - Immunology 2020Quote: ... Supernatants were harvested 48 hours after culture and analyzed for IFNβ by ELISA (Biolegend) according to manufacturer protocol.
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bioRxiv - Immunology 2020Quote: ... supernatants were harvested and TNF and IL-2 levels were measured by ELISA (Biolegend) according to the manufacturer’s protocol ...
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bioRxiv - Pathology 2022Quote: ... the resulting supernatant was harvested and used to quantify IL-10 by ELISA (Biolegend), or to test its capacity to block BV2 polarisation in the presence of E ...
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bioRxiv - Biochemistry 2020Quote: ... Human ucOCN measurements were performed using a commercially available human ucOCN ELISA (BioLegend, 446707) (Lacombe et al. ...
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bioRxiv - Microbiology 2021Quote: ... Secreted TNF-α was quantified in supernatants by enzyme-linked immunosorbent assay (ELISA; BioLegend).
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bioRxiv - Synthetic Biology 2022Quote: ... and IL-2 or IFNγ were measured by ELISA following the manufacturer’s protocol (BioLegend). Absorbances were measured with a Synergy H1 Hybrid Multi-Mode Reader (BioTek).
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bioRxiv - Immunology 2022Quote: ... in PBS were added to 96-well high-bound Nunc MaxiSorp ELISA plates (BioLegend) and incubated at 4°C overnight ...
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bioRxiv - Immunology 2023Quote: ... IL-2 concentration in thawed supernatant was measured by ELISA (Biolegend, IL-2 human). For experiments with KIR2DS1 reporter cells ...
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bioRxiv - Biophysics 2024Quote: ... The supernatant was used for triplicate samples in the ELISA (Biolegend, San Diego CA), carried out according to manufacturers instructions.
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bioRxiv - Immunology 2024Quote: IL-6 ELISA was performed according to the manufacturer’s instruction (Biolegend, Cat. No. 431301) with mouse serum diluted 1:50 ...
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bioRxiv - Cancer Biology 2024Quote: ... blocked for 1 hour at RT with 1x ELISA dilutant buffer (Biolegend Cat# 421203), then washed twice with 1x wash buffer ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were then stained for transferrin receptor expression by adding 0.25 μg of PE/Cy7 antihuman CD71 [CY1G4] (Biolegend, Cat# 334111) directly to the blocking solution for 30 min at room temperature ...
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bioRxiv - Cancer Biology 2021Quote: ... resuspended in 100 µl of FACS buffer and incubated in 5 µl of Human TruStain FcX™ (Fc receptor blocking solution, BioLegend) for 10 minutes at RT ...
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bioRxiv - Immunology 2021Quote: ... Cells were stained for 10 minutes at room temperature with Ghost Dye Violet 510 and Fc receptor blocking solution (Human TruStain FcX™, BioLegend) and washed once in FACS buffer ...
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bioRxiv - Immunology 2021Quote: ... cells were initially blocked with anti-mouse Fcγ III/II receptor (CD16/CD32) antibodies and dead cells excluded by staining with a fixable live/dead cell-exclusion dye (BioLegend, USA). Then ...
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bioRxiv - Genomics 2021Quote: Both iMGs and oMGs were resuspended in staining buffer and were blocked with Fc receptor blocking antibody (Human TruStain FcX, BioLegend 422302) for 10 minutes ...
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bioRxiv - Immunology 2022Quote: ... Blocking and antibody solutions were further supplemented with 1:150 Human TruStain FcX™ Fc Receptor Blocking Solution (BioLegend, London, UK), to reduce non-specific binding ...
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bioRxiv - Immunology 2023Quote: ... Cells were pelleted at 500xg for 5 minutes and then resuspended in 50uL of PBS+ 2% HI-FBS + 2mM EDTA in the presence of Fc receptor blocking antibody (BioLegend, 101302). Cells were labelled with antibodies as described below ...
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bioRxiv - Immunology 2023Quote: ... Cells were stained with Zombie-violet or Zombie-NIR in PBS at 1:2000 dilution for 5 minutes at room temperature prior to resuspending in PBS + 2% HI-FBS + 2mM EDTA in the presence of Fc receptor blocking antibody (BioLegend, 101302) for blocking ...
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bioRxiv - Microbiology 2023Quote: ... Each well was blocked using 50 μL of FACS buffer (PBS + 5% FBS) + 2.5 μL Fc receptor (BioLegend, Cat No. 422302) block per well and then 150 μL FACS buffer was added and spun down ...
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bioRxiv - Bioengineering 2023Quote: ... Cells were then stained for transferrin receptor expression by adding 0.25 μg of PE/Cy7 anti-human CD71 [CY1G4] (Biolegend, Cat# 334111) directly to the blocking solution for 30 min at room temperature ...
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bioRxiv - Immunology 2023Quote: ... Cells were rinsed with FACS buffer (PBS containing 0.5% BSA and 2mM EDTA) for 3 min at 300g and incubated with Fc Receptor Blocking solution (Human TruStain FcX™, Biolegend) for 10 minutes prior to cell surface staining with fluorescently labeled antibodies in Brilliant Stain buffer (BD Biosciences ...
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bioRxiv - Immunology 2023Quote: ... stained for 10 minutes at room temperature with Ghost Dye Violet 510 and Fc receptor blocking solution (Human TruStain FcX™, BioLegend), and washed once in FACS buffer ...
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bioRxiv - Immunology 2021Quote: ... Cell sorts were performed with MojoSort Mouse Pan B Isolation Kit II (BioLegend, San Diego, CA) for CD19+ cells and with Miltenyi’s magnetic-activated cell sorting (MACS ...
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bioRxiv - Immunology 2021Quote: ... cells were purified using negative magnetic bead selection (MojoSort Mouse Pan Dendritic Cell Isolation Kit, BioLegend). 2.5×106 BMDCs per well were cultured for 6h in 6-well plates in RPMI complete (R10 ...
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bioRxiv - Cancer Biology 2022Quote: ... MCT-treated CD11b+ cells were isolated from tumors using MojoSort Mouse CD11b Selection Kit (BioLegend, 480109). For transfecting every million primary CD11b+ cells ...