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Citations for BioLegend :
601 - 650 of 5739 citations for Mouse VPS10 domain containing receptor SorCS1 SORCS1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: Cells were pelleted and the undiluted supernatant was tested by ELISA (BioLegend) for the monocyte-attractive ...
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bioRxiv - Neuroscience 2022Quote: ... Cells were then incubated with Brilliant Violet 421-labelled anti-human P2Y12 receptor antibody (clone S16001E, Biolegend, Cat# 392106) or with IgG2a isotype control antibody (clone MOPC-173 ...
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bioRxiv - Immunology 2020Quote: ... Non-specific binding was blocked by incubating cells with human anti-Fc Receptor antibodies (TrueStain, Biolegend, Cat. No. 101320) or anti-mouse CD16/32 Fc block (Biolegend ...
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bioRxiv - Immunology 2021Quote: ... Cells were incubated at 4°C for 15 minutes with an Fc receptor blocker (BioLegend TruStain FcX, cat # 422302) according to manufacturer instructions before cocktails of surface antibodies were added for 30 minutes at 4°C ...
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bioRxiv - Bioengineering 2022Quote: ... and then stained with the fluorescently labeled antibody that detects the corresponding cell surface receptor (anti-EGFR-BV421, Biolegend, Cat ...
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bioRxiv - Immunology 2020Quote: ... Blocking of Fc receptors in T2 cells was performed prior to staining using the TruStain FcX reagent (BioLegend, #422301). Staining with peptide-MHC dextramers was performed for 10 min at room temperature (RT) ...
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BCR analysis of single-sorted, putative IgE+ memory B cells in food allergy: an ephemeral existence?bioRxiv - Immunology 2019Quote: ... In all assays 1 x 106 cells were first incubated with Human TruStain FcX (Fc Receptor Blocking Solution, Biolegend) or anti-human CD32 (FcγRII Blocker ...
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bioRxiv - Immunology 2020Quote: ... stained for 20 min at 4 °C in PBS/2% FCS in presence of Fc receptor blocking reagent (Biolegend) with anti-murine CD326-BV605 ...
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bioRxiv - Immunology 2024Quote: ... PBMCs were thawed and staining for surface antigens was performed following Fc receptor blockade with Human TruStain FcX (BioLegend). Central/effector memory CD4+ T cells were defined as live/CD16-/CD14-/CD123-/CD56-/CD11c-/CD19-/CD4+ > excluding CD25hiCD127lo > CD45RA-/CD27+ ...
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bioRxiv - Cancer Biology 2024Quote: ... Barcoded cells were combined into a composite sample before incubation in Human TruStain FcX Fc Receptor Blocking Solution (BioLegend) for 10 min at room temperature ...
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bioRxiv - Immunology 2023Quote: ... CD141 BV605(Clone:M80 Cat:344117) in the presence of human anti-Fc Receptor antibodies (TrueStain; Biolegend; cat. no. 101320). cDCs1 were FACS-sorted as CD45+Lin- (Lin:CD3 ...
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bioRxiv - Immunology 2023Quote: ... The CD16/CD32 receptors were then blocked by incubating with a 1/50 dilution of TruStain FcX™ (BioLegend) in PBS at room temperature for 15 min ...
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bioRxiv - Bioengineering 2023Quote: ... cells were washed with FACS buffer (PBS + 2% FBS + 0.05% NaN3) and incubated with Fc receptor blocking solution (Human TruStain FcX, BioLegend) for 5 minutes ...
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bioRxiv - Immunology 2024Quote: ... and mesenteric lymph nodes were first treated with Fc receptor block (anti-CD16/CD32 clone 2.4G2) and then stained using the following fluorochrome-conjugated antibodies purchased from Biolegend, eBioscience ...
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bioRxiv - Immunology 2023Quote: CD3+ T cells were purified from BALB/c mouse spleen cells with the MojoSort mouse CD3 T cell isolation kit (Biolegend, San Diego, CA) according to the manufacturer’s instructions ...
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bioRxiv - Genetics 2020Quote: ... Following overnight incubation yeast were washed once in PBS/0.2% BSA and rotated at 18 rpm in same buffer containing 5 µg/mL mouse anti-His6 IgG (BioLegend, San Diego, CA) that had been conjugated with Alexa647 using the N-hydroxysuccinimidyl ester (Molecular Probes ...
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bioRxiv - Cell Biology 2022Quote: Whole lung single cell suspensions were prepared as above and then blocked in SB containing 1:50 TruStain FcX™ (anti-mouse CD16/32) Antibody (BioLegend, #101319) for 10 min at 37 °C ...
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bioRxiv - Cell Biology 2021Quote: ... and then resuspended in 10% FCS in PBS (100μl/well) containing TruStain FcX™ (anti-mouse CD16/32) antibody (Biolegend; 1:200) and incubated on ice for 20mins ...
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bioRxiv - Immunology 2020Quote: ... For investigating the formation of iBALT B cell and T cells were stained in lungs using primary antibody cocktail containing rat anti-mouse CD45R/B220 (BioLegend, Cat#103202) and Hamster anti mouse CD3 (BD ...
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bioRxiv - Bioengineering 2022Quote: ... 100 µl of 105/ml aAPC-BLMs containing all three ligands were stained with 5 µl of anti-mouse IgG PE (BioLegend, Cat#406607) and anti-human HLA-DR PE (BioLegend ...
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bioRxiv - Cancer Biology 2022Quote: ... Cancer cells were purified using the MojoSort Mouse CD326/EpCAM Selection Kit (BioLegend, 480141), followed by RNA extraction using RNeasy Kit (Qiagen) ...
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bioRxiv - Immunology 2021Quote: ... BM cells were enriched for neutrophils using a mouse neutrophil negative enrichment kit (Biolegend).
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bioRxiv - Immunology 2024Quote: CD4+ T cells were isolated with MojoSortTM Mouse CD4 T cell isolation kit (Biolegend) from spleens of WT mice with > 90% purity ...
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bioRxiv - Immunology 2023Quote: CD4+ T cells were isolated using MojoSort Mouse CD4 T cell isolation kit (BioLegend). Upon isolation ...
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bioRxiv - Immunology 2023Quote: ... Memory cells were obtained from spleens using the MojoSort Mouse CD45.1 selection kit (BioLegend) >40 days post infection.
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bioRxiv - Immunology 2024Quote: ... isolated to >95% purity using the MojoSort™ Mouse Neutrophil Isolation Kit (480058, BioLegend) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... TNF-α or caspase-1 by ELISA (Biolegend Inc., San Diego, CA, EUA).
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bioRxiv - Microbiology 2023Quote: ... IL-1β and IL-6 were analyzed by ELISA MAX Deluxe Sets (Biolegend) according to manufacturer’s instruction.
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bioRxiv - Neuroscience 2023Quote: ... Supernatants were collected and used for cytokine ELISA assays per manufacturer’s instructions (Biolegend). Total RNAs were prepared from collected cells using Trizol (Invitrogen ...
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bioRxiv - Immunology 2023Quote: ... plasma was tested with human IL-6 ELISA MAX Deluxe Set from Biolegend.
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bioRxiv - Neuroscience 2024Quote: ... Supernatants were collected and used for cytokine ELISA assays per manufacturer’s instructions (Biolegend). Total RNAs were prepared from collected cells using Trizol (Invitrogen ...
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bioRxiv - Synthetic Biology 2021Quote: ... The knock-out of the T cell receptor (TCR) was assessed with the absence of signal after staining 1:200 with APC-labeled anti-CD3ε (Biolegend). Each CAR construct contained a Strep tag which allowed for a two-step staining to validate successful knock-in ...
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bioRxiv - Cell Biology 2020Quote: ... Primary antibodies targeting the various surface ligands and receptors as well as the corresponding control include: FITC anti-human HLA-A,B,C (Clone W6/32, Biolegend), FITC anti-human ICAM-1/CD54 (Clone DX2 ...
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bioRxiv - Microbiology 2019Quote: ... Human B cells were incubated in human Fc receptor blocking antibody then stained with Zombie Aqua fixable viability dye (1:500 dilution) (BioLegend), according to manufacturer’s protocol ...
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bioRxiv - Neuroscience 2021Quote: ... Single Cell suspensions were incubated with an anti-FcγIII/II receptor antibody (clone 93, eBioscience) to block unspecific binding and Zombie NIR™ (Biolegend), a fixable viability dye ...
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bioRxiv - Microbiology 2021Quote: ... Cells were filtered through a 70 µm strainer and red blood cells were lysed before blocking FC receptors using anti-CD16/CD32 (BioLegend). Cell-specific proteins were labeled with the following antibodies ...
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bioRxiv - Bioengineering 2022Quote: ... and then stained with the fluorescently labeled antibody that detects the corresponding cell surface receptor (anti-EGFR-BV421, Biolegend, Cat: 352911; anti-HER2-BV421, Biolegend, Cat ...
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Cholesterol deprivation induces TGFβ signaling to promote basal differentiation in pancreatic cancerbioRxiv - Cancer Biology 2019Quote: ... To prevent antibodies from binding to Fc-receptors we treated the cell suspension with Fc-block (Biolegend #101301; 1:50) prior using other antibodies ...
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bioRxiv - Immunology 2020Quote: ... Cells were resuspended in their residual buffer before being incubated with anti-CD16/CD32 (‘Fc Block’) to reduce non-specific binding to Fc receptors (Biolegend). After 5 min ...
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bioRxiv - Immunology 2021Quote: ... Single cell suspensions were blocked with rat anti-mouse FcγIII/II receptor (CD16/CD32) blocking antibodies (“Fc-Block”) and stained with live/dead cell-exclusion dye (Zombie UV dye; Biolegend). The cells were then incubated with fluorophore-conjugated antibodies directed against cell surface antigens ...
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bioRxiv - Molecular Biology 2023Quote: ... 10 million cells were resuspended in 1 mL of FACS buffer and 50 μL of Human TruStain FcX Fc Receptor Blocking Solution (BioLegend) was added and left to bind the cells on ice for 15 minutes ...
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bioRxiv - Genomics 2023Quote: ... Cells were pre-incubated with 5 µl of Human TruStain FcX™ (Fc Receptor Blocking Solution, BioLegend Cat. No. 422301) per 100µl of cell suspension for 5–10 minutes at room temperature ...
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bioRxiv - Immunology 2023Quote: ... Cells were incubated with anti-CD16/CD32 (clone 93, eBioscience, 14-0161-86) for Fc receptor blockade and stained with Zombie Yellow (Biolegend) for 10 minutes at room temperature in the dark to distinguish live and dead cells ...
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bioRxiv - Immunology 2023Quote: ... single cell suspensions were incubated on ice for 10 minutes with Fc receptor-blocking anti-CD16/32 (BioLegend Cat# 101301). Cell suspensions were first stained for 5 minutes at room temperature with LIVE/DEAD® Fixable Red Dead Cell Stain Kit (ThermoFisher Cat# L23102) ...
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bioRxiv - Cell Biology 2024Quote: ... FBM and ABM cells were resuspended in ice-cold PBS with 5ul of Fc receptor blocker (Human TruStain FcX, Biolegend) and were incubated for 10 min at 4°C ...
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bioRxiv - Immunology 2024Quote: ... for Fc receptors blocking and then simultaneously incubated with HpARI proteins tetramers and stained for CD45 (APC-Cy7, clone 30-F11; Biolegend). Cells were then acquired on a LSR Fortressa (BD ...
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bioRxiv - Immunology 2021Quote: ... cells were washed with staining buffer (PBS containing 2% FBS) and surface stained with anti-mouse-CD4-PerCP/Cy5.5 (Biolegend Cat# 116012, RRID: AB_2563023), anti-mouse-CD8-BV785 (Biolegend Cat# 100750 ...
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bioRxiv - Immunology 2024Quote: ... antibody or 100µL of PBS containing 0.2µg TruStain FcX™ and 0.04µL Zombie AquaTM Fixable Viability Kit (BioLegend cat#: 423102) and incubated at room temperature (RT ...
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bioRxiv - Cancer Biology 2020Quote: ... and plasma were assayed for CXCL5 using a human/mouse CXCL5 immunoassay kit (BioLegend, 440904).
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bioRxiv - Developmental Biology 2019Quote: ... placental single-cell suspensions were stained with APC-conjugated anti-mouse CD117 (c-kit) (BioLegend), PerCP/Cy5.5-conjugated anti-mouse CD34 (BioLegend) ...